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The map label for this gene is trmB

Identifier: 110835434

GI number: 110835434

Start: 2913993

End: 2914712

Strand: Direct

Name: trmB

Synonym: ABO_2573

Alternate gene names: 110835434

Gene position: 2913993-2914712 (Clockwise)

Preceding gene: 110835433

Following gene: 110835437

Centisome position: 93.39

GC content: 57.08

Gene sequence:

>720_bases
ATGCTCGATTTCATCAACCACGATAAAGATCCGGAAACGGGGAAAGTCATGCGTAAAGTGCGCAGCTTTGTGCTGCGCGA
AGGCCGCCTGACCGCCGGACAACGCAATGCTTTGGACACCCTGTGGCCCCGCTTTGGGCTGGAGCGCGACCAAGGCATGC
TCAACCCCGAAAGCGTTTTCGGTCGCGACGCCCCCCGGGTTCTCGAAATCGGATACGGCATGGGCCAATCGCTGGCGCAA
ATGGCTGCCGCCGATCCCGATAAGGACTTTATCGGTATTGAAGTGCATCGCCCCGGCGTAGGCGCGCTGCTTATGGAGAT
TGAGCAACAGGGCCTAAGCAACCTGCGCAGCTACTGTGACGATGCGGTAGAGATTCTTGAGCTGTGCATCCCCGACAACA
GCTTGGCGCGGGTTCAACTGTATTTCCCCGATCCTTGGCACAAAAAAAAGCACCACAAGCGCCGAATCGTTCAGCCCGCT
TGGGTGGCTTTGGTGCAGCGCAAACTTCAGCCAGGAGGCATTCTGCACATGGCCACGGACTGGGAAAATTATGCAGAGCA
CATGATGGAAGTGATGGATGCGGCCGCAGGGTTTAGCAACTTGGCAGGCCCAAGCGCATTTTCTCCCCGGCCCAGCTGGC
GCCCGGAAACCAAGTTTGAACGCCGCGGCGAAAAATTGGGCCATGGGGTGTGGGATTTATTATTCGAGAAAAGGGCTTAA

Upstream 100 bases:

>100_bases
TGAAGGCCACACCCTGTGGCCCCGCTGTCGCCCTTTTTTTCAATGGACGGTTATAATCCGCCCTTGATTACTTTCGCCTC
TCATACGGTAACTCGGCACC

Downstream 100 bases:

>100_bases
CAGAAACGCATTCAGCTTAAACACGGTCATAAAAAACCGCAGCTAAGGCTGCGGTTTTTCTTTATTCACCCTTAGTAGAA
ATAGCCTTCCAAGCCAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLDFINHDKDPETGKVMRKVRSFVLREGRLTAGQRNALDTLWPRFGLERDQGMLNPESVFGRDAPRVLEIGYGMGQSLAQ
MAAADPDKDFIGIEVHRPGVGALLMEIEQQGLSNLRSYCDDAVEILELCIPDNSLARVQLYFPDPWHKKKHHKRRIVQPA
WVALVQRKLQPGGILHMATDWENYAEHMMEVMDAAAGFSNLAGPSAFSPRPSWRPETKFERRGEKLGHGVWDLLFEKRA

Sequences:

>Translated_239_residues
MLDFINHDKDPETGKVMRKVRSFVLREGRLTAGQRNALDTLWPRFGLERDQGMLNPESVFGRDAPRVLEIGYGMGQSLAQ
MAAADPDKDFIGIEVHRPGVGALLMEIEQQGLSNLRSYCDDAVEILELCIPDNSLARVQLYFPDPWHKKKHHKRRIVQPA
WVALVQRKLQPGGILHMATDWENYAEHMMEVMDAAAGFSNLAGPSAFSPRPSWRPETKFERRGEKLGHGVWDLLFEKRA
>Mature_239_residues
MLDFINHDKDPETGKVMRKVRSFVLREGRLTAGQRNALDTLWPRFGLERDQGMLNPESVFGRDAPRVLEIGYGMGQSLAQ
MAAADPDKDFIGIEVHRPGVGALLMEIEQQGLSNLRSYCDDAVEILELCIPDNSLARVQLYFPDPWHKKKHHKRRIVQPA
WVALVQRKLQPGGILHMATDWENYAEHMMEVMDAAAGFSNLAGPSAFSPRPSWRPETKFERRGEKLGHGVWDLLFEKRA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=235, Percent_Identity=57.8723404255319, Blast_Score=285, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_ALCBS (Q0VLC7)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_694293.1
- ProteinModelPortal:   Q0VLC7
- SMR:   Q0VLC7
- STRING:   Q0VLC7
- GeneID:   4213307
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2573
- NMPDR:   fig|393595.12.peg.2580
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q0VLC7
- BioCyc:   ABOR393595:ABO_2573-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27172; Mature: 27172

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 94-94 BINDING 121-121 BINDING 144-144 BINDING 148-148 BINDING 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFINHDKDPETGKVMRKVRSFVLREGRLTAGQRNALDTLWPRFGLERDQGMLNPESVF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
GRDAPRVLEIGYGMGQSLAQMAAADPDKDFIGIEVHRPGVGALLMEIEQQGLSNLRSYCD
CCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
DAVEILELCIPDNSLARVQLYFPDPWHKKKHHKRRIVQPAWVALVQRKLQPGGILHMATD
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECC
WENYAEHMMEVMDAAAGFSNLAGPSAFSPRPSWRPETKFERRGEKLGHGVWDLLFEKRA
HHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MLDFINHDKDPETGKVMRKVRSFVLREGRLTAGQRNALDTLWPRFGLERDQGMLNPESVF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
GRDAPRVLEIGYGMGQSLAQMAAADPDKDFIGIEVHRPGVGALLMEIEQQGLSNLRSYCD
CCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
DAVEILELCIPDNSLARVQLYFPDPWHKKKHHKRRIVQPAWVALVQRKLQPGGILHMATD
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECC
WENYAEHMMEVMDAAAGFSNLAGPSAFSPRPSWRPETKFERRGEKLGHGVWDLLFEKRA
HHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA