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The map label for this gene is thiG [H]

Identifier: 110835433

GI number: 110835433

Start: 2912974

End: 2913753

Strand: Direct

Name: thiG [H]

Synonym: ABO_2572

Alternate gene names: 110835433

Gene position: 2912974-2913753 (Clockwise)

Preceding gene: 110835432

Following gene: 110835434

Centisome position: 93.36

GC content: 56.67

Gene sequence:

>780_bases
ATGTCCGATCTGCTTACCATTGCCGGAAAAACCTACTCCTCCCGCCTGCTGATCGGCACCGGCAAGTACAAAGACTTTGA
CGAAACCCGCGAATCCATCGAAATCAGCGGCGCGGAAATCGTTACCGTCGCCATTCGCCGCACCAATATTGGCCAAAACG
CAGACGAACCCAATCTGCTCGATTTCGTCAGCCCCGACAAATACACCATTTTGCCTAACACCGCCGGCTGCTTCTCCGCC
GAAGATGCCGTGCGTTGCTGCAAACTGGCTCGGGAGCTGCTTGATGGCCATAGCTTGGTCAAATTGGAGGTACTCGGCGA
TCAGAAAACTCTGTACCCGAACATCACCCATACCTTGCACGCCGCCCGCGAACTGATCGACGACGGCTTCAACGTCATGG
TCTACACCAACGACGACCCCATCGTGGCGAAAGAGCTGGAAGATATGGGGTGTGTGGCAGTAATGCCGCTGGGCTCATTA
ATCGGCTCCGGGCTTGGCATCCTTAACCCTCACAACATCAAGCTCATCATGGAAGAAGCCAACGTGCCCATCATTGTCGA
TGCCGGCGTCGGCACCGCTTCCGATGCCGCACTGGCCATGGAAATGGGTTGCGATGGGGTGCTACTCAACTCTGCCGTGG
CCCATGCACAAAAGCCGGTACTAATGGCTTCCGCCATGAAGAAAGCCATTGAAGCTGGCCGCGAAGCTTTTCTGGCCGGG
CGCATGCCTCGCAAGGCCTATGCCTCTGCCTCATCGCCACTGGATGGTCTGAGCCGCTAA

Upstream 100 bases:

>100_bases
CGCCTTAGCCAATAGGTCCAGGCGCCCCCTGAGTTCCTGTGCAGGTTCCAAGGCTGCCGGAAAAACCTGATAAAAGACGT
TAATACCCTAAGAGGCCCCC

Downstream 100 bases:

>100_bases
GCCTTTCGACAGACAAAAAAACGGCGCCACTCTGGAGGGAGTTGTCGCCAAGGGGGGAAATCATCGATGCTCGTGCACAT
AAGGGCAGAGTTGCGTTCAC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSDLLTIAGKTYSSRLLIGTGKYKDFDETRESIEISGAEIVTVAIRRTNIGQNADEPNLLDFVSPDKYTILPNTAGCFSA
EDAVRCCKLARELLDGHSLVKLEVLGDQKTLYPNITHTLHAARELIDDGFNVMVYTNDDPIVAKELEDMGCVAVMPLGSL
IGSGLGILNPHNIKLIMEEANVPIIVDAGVGTASDAALAMEMGCDGVLLNSAVAHAQKPVLMASAMKKAIEAGREAFLAG
RMPRKAYASASSPLDGLSR

Sequences:

>Translated_259_residues
MSDLLTIAGKTYSSRLLIGTGKYKDFDETRESIEISGAEIVTVAIRRTNIGQNADEPNLLDFVSPDKYTILPNTAGCFSA
EDAVRCCKLARELLDGHSLVKLEVLGDQKTLYPNITHTLHAARELIDDGFNVMVYTNDDPIVAKELEDMGCVAVMPLGSL
IGSGLGILNPHNIKLIMEEANVPIIVDAGVGTASDAALAMEMGCDGVLLNSAVAHAQKPVLMASAMKKAIEAGREAFLAG
RMPRKAYASASSPLDGLSR
>Mature_258_residues
SDLLTIAGKTYSSRLLIGTGKYKDFDETRESIEISGAEIVTVAIRRTNIGQNADEPNLLDFVSPDKYTILPNTAGCFSAE
DAVRCCKLARELLDGHSLVKLEVLGDQKTLYPNITHTLHAARELIDDGFNVMVYTNDDPIVAKELEDMGCVAVMPLGSLI
GSGLGILNPHNIKLIMEEANVPIIVDAGVGTASDAALAMEMGCDGVLLNSAVAHAQKPVLMASAMKKAIEAGREAFLAGR
MPRKAYASASSPLDGLSR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=43.3070866141732, Blast_Score=200, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27605; Mature: 27474

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLTIAGKTYSSRLLIGTGKYKDFDETRESIEISGAEIVTVAIRRTNIGQNADEPNLL
CCCCEEECCCCCCCEEEEECCCCCCHHHHHHHEEECCCEEEEEEEEECCCCCCCCCCCEE
DFVSPDKYTILPNTAGCFSAEDAVRCCKLARELLDGHSLVKLEVLGDQKTLYPNITHTLH
ECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCHHHHHH
AARELIDDGFNVMVYTNDDPIVAKELEDMGCVAVMPLGSLIGSGLGILNPHNIKLIMEEA
HHHHHHHCCCEEEEEECCCCCHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCEEEEEECC
NVPIIVDAGVGTASDAALAMEMGCDGVLLNSAVAHAQKPVLMASAMKKAIEAGREAFLAG
CCCEEEECCCCCCCCHHEEECCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
RMPRKAYASASSPLDGLSR
CCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDLLTIAGKTYSSRLLIGTGKYKDFDETRESIEISGAEIVTVAIRRTNIGQNADEPNLL
CCCEEECCCCCCCEEEEECCCCCCHHHHHHHEEECCCEEEEEEEEECCCCCCCCCCCEE
DFVSPDKYTILPNTAGCFSAEDAVRCCKLARELLDGHSLVKLEVLGDQKTLYPNITHTLH
ECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCHHHHHH
AARELIDDGFNVMVYTNDDPIVAKELEDMGCVAVMPLGSLIGSGLGILNPHNIKLIMEEA
HHHHHHHCCCEEEEEECCCCCHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCEEEEEECC
NVPIIVDAGVGTASDAALAMEMGCDGVLLNSAVAHAQKPVLMASAMKKAIEAGREAFLAG
CCCEEEECCCCCCCCHHEEECCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
RMPRKAYASASSPLDGLSR
CCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA