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The map label for this gene is pfkA [H]

Identifier: 110834850

GI number: 110834850

Start: 2275917

End: 2277179

Strand: Direct

Name: pfkA [H]

Synonym: ABO_1989

Alternate gene names: 110834850

Gene position: 2275917-2277179 (Clockwise)

Preceding gene: 110834848

Following gene: 110834854

Centisome position: 72.94

GC content: 55.42

Gene sequence:

>1263_bases
ATGGCGCGTAAAAACGCTTTCTATGCTCAGTCCGGCGGTGTTACTGCCGTCATCAATGCTTCTGCTGCCGGCGTTATTGA
AGCAGCACGAGAAAACAAGGATGTGATCGGCAAAGTCTACGCCGGCCGGAATGGCATCATCGGCGCACTCACTGAAGAGC
TGATCGATACAAGCAAAGAAAGCAAAAGCGCCATTGAAGCGCTCAAGCACACCCCCGGCGGGGCCTTCGGTTCTTGCCGC
TATAAGCTGAAAGACCTCAAGACCTCCCAACGCGAATACGAACGTCTAATCGAAGTCTTCAAGGCTCACAACATCGGCTA
CTTCTTCTATAACGGTGGTGGTGATTCCGCCGACACCTGCCTAAAAGTATCACAGCTTTCCGAGAAAATGGGCTACCCGA
TCCAGGCTATCCACGTGCCCAAAACCGTGGACAACGACCTGCCCTTCACCGACGTGAGCCCTGGTTTCGGTTCCGTCGCC
AAGTACACCGCTGTGAGCTGCCGTGAGGCCGCCCTGGACGTGGCCAGCATGTGCGCCACCTCCACCAAGGTGTTCGTGAT
GGAAGTCATGGGCCGTCACGCTGGCTGGATCGCCGCCTCCGCCGGGCTCGCCAAAGAGGATGCCGATGACGCTCCGCACC
TAATCTTGTTCCCGGAAATTGCCTTCGACAAAGCGGCCTTCATGAAAAAGGTGGAAGCCACCGTCAAGAAAGCGGGCTAT
TGCGTCATTGTGGTGTCCGAAGGGGCTCGCTATGAAGACGGCACGTTCTTGGCCGACGCGGGTAACACTGACGCCTTTGG
CCACAAACAGCTAGGCGGTGTGGCTCCGGTCATCGCTCAGATGGTGAAAGACGAACTCGGCTACAAATACCACTGGGCTG
TAAGCGATTACTTACAGCGTGCCGCACGCCACATAGCATCAGCCACTGACGTGGAGCAAGCCTACGCAGTGGGCCGCGCC
GCCGTTGAGTTCGCCGTGGCGGGTAAAAACGCAGTCATGCCCACCGTGGTTCGCAAGGACACAAAAAAATACAGCTGGAC
CATCGGCGAAGCGCCGCTAAAGAAAGTGGCCAATGTAGAAAAGAAAATGCCGCGTAAATACATTAGCAAAGATGGTTTCG
GCATTACCCCCGCGGCCCGAGCCTACCTAGAGCCTTTGATTGGTGGTGAATCCTACCCTCCCTACAAAAACGGGCTGCCG
CAATTTGCCAAACTAAAACTAGCCCCAGTCGAGAAAAAGTTAAACGATGCTTTTGAGGTCTAA

Upstream 100 bases:

>100_bases
TGCACACCTTGAACCTTGACACGATATGGTCCGACAACCACAGGTGTGTTTTTTGGACGCCTTCGCTAGAATGCTCACAA
ATTGAATCAAGGGGTCTCAC

Downstream 100 bases:

>100_bases
AACACTAGCATCTTGATAAAAAGCAAAAGCCTCGCAATGCGAGGCTTTTGCTTTTCACTAACGGCCAATGGCCATTCAGC
CCTGACCGTTTCCCAGAACA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MARKNAFYAQSGGVTAVINASAAGVIEAARENKDVIGKVYAGRNGIIGALTEELIDTSKESKSAIEALKHTPGGAFGSCR
YKLKDLKTSQREYERLIEVFKAHNIGYFFYNGGGDSADTCLKVSQLSEKMGYPIQAIHVPKTVDNDLPFTDVSPGFGSVA
KYTAVSCREAALDVASMCATSTKVFVMEVMGRHAGWIAASAGLAKEDADDAPHLILFPEIAFDKAAFMKKVEATVKKAGY
CVIVVSEGARYEDGTFLADAGNTDAFGHKQLGGVAPVIAQMVKDELGYKYHWAVSDYLQRAARHIASATDVEQAYAVGRA
AVEFAVAGKNAVMPTVVRKDTKKYSWTIGEAPLKKVANVEKKMPRKYISKDGFGITPAARAYLEPLIGGESYPPYKNGLP
QFAKLKLAPVEKKLNDAFEV

Sequences:

>Translated_420_residues
MARKNAFYAQSGGVTAVINASAAGVIEAARENKDVIGKVYAGRNGIIGALTEELIDTSKESKSAIEALKHTPGGAFGSCR
YKLKDLKTSQREYERLIEVFKAHNIGYFFYNGGGDSADTCLKVSQLSEKMGYPIQAIHVPKTVDNDLPFTDVSPGFGSVA
KYTAVSCREAALDVASMCATSTKVFVMEVMGRHAGWIAASAGLAKEDADDAPHLILFPEIAFDKAAFMKKVEATVKKAGY
CVIVVSEGARYEDGTFLADAGNTDAFGHKQLGGVAPVIAQMVKDELGYKYHWAVSDYLQRAARHIASATDVEQAYAVGRA
AVEFAVAGKNAVMPTVVRKDTKKYSWTIGEAPLKKVANVEKKMPRKYISKDGFGITPAARAYLEPLIGGESYPPYKNGLP
QFAKLKLAPVEKKLNDAFEV
>Mature_419_residues
ARKNAFYAQSGGVTAVINASAAGVIEAARENKDVIGKVYAGRNGIIGALTEELIDTSKESKSAIEALKHTPGGAFGSCRY
KLKDLKTSQREYERLIEVFKAHNIGYFFYNGGGDSADTCLKVSQLSEKMGYPIQAIHVPKTVDNDLPFTDVSPGFGSVAK
YTAVSCREAALDVASMCATSTKVFVMEVMGRHAGWIAASAGLAKEDADDAPHLILFPEIAFDKAAFMKKVEATVKKAGYC
VIVVSEGARYEDGTFLADAGNTDAFGHKQLGGVAPVIAQMVKDELGYKYHWAVSDYLQRAARHIASATDVEQAYAVGRAA
VEFAVAGKNAVMPTVVRKDTKKYSWTIGEAPLKKVANVEKKMPRKYISKDGFGITPAARAYLEPLIGGESYPPYKNGLPQ
FAKLKLAPVEKKLNDAFEV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=242, Percent_Identity=26.0330578512397, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 45264; Mature: 45133

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKNAFYAQSGGVTAVINASAAGVIEAARENKDVIGKVYAGRNGIIGALTEELIDTSKE
CCCCCCEEECCCCEEEEEECCHHHHHHHHHCCCHHEEEEECCCCCCHHHHHHHHHHCCHH
SKSAIEALKHTPGGAFGSCRYKLKDLKTSQREYERLIEVFKAHNIGYFFYNGGGDSADTC
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
LKVSQLSEKMGYPIQAIHVPKTVDNDLPFTDVSPGFGSVAKYTAVSCREAALDVASMCAT
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
STKVFVMEVMGRHAGWIAASAGLAKEDADDAPHLILFPEIAFDKAAFMKKVEATVKKAGY
CHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCE
CVIVVSEGARYEDGTFLADAGNTDAFGHKQLGGVAPVIAQMVKDELGYKYHWAVSDYLQR
EEEEECCCCCCCCCCEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHH
AARHIASATDVEQAYAVGRAAVEFAVAGKNAVMPTVVRKDTKKYSWTIGEAPLKKVANVE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHH
KKMPRKYISKDGFGITPAARAYLEPLIGGESYPPYKNGLPQFAKLKLAPVEKKLNDAFEV
HHCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCHHHHEEECHHHHHHHHHHCC
>Mature Secondary Structure 
ARKNAFYAQSGGVTAVINASAAGVIEAARENKDVIGKVYAGRNGIIGALTEELIDTSKE
CCCCCEEECCCCEEEEEECCHHHHHHHHHCCCHHEEEEECCCCCCHHHHHHHHHHCCHH
SKSAIEALKHTPGGAFGSCRYKLKDLKTSQREYERLIEVFKAHNIGYFFYNGGGDSADTC
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
LKVSQLSEKMGYPIQAIHVPKTVDNDLPFTDVSPGFGSVAKYTAVSCREAALDVASMCAT
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
STKVFVMEVMGRHAGWIAASAGLAKEDADDAPHLILFPEIAFDKAAFMKKVEATVKKAGY
CHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCE
CVIVVSEGARYEDGTFLADAGNTDAFGHKQLGGVAPVIAQMVKDELGYKYHWAVSDYLQR
EEEEECCCCCCCCCCEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHH
AARHIASATDVEQAYAVGRAAVEFAVAGKNAVMPTVVRKDTKKYSWTIGEAPLKKVANVE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHH
KKMPRKYISKDGFGITPAARAYLEPLIGGESYPPYKNGLPQFAKLKLAPVEKKLNDAFEV
HHCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCHHHHEEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]