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The map label for this gene is amiB [H]

Identifier: 110834840

GI number: 110834840

Start: 2263548

End: 2264585

Strand: Reverse

Name: amiB [H]

Synonym: ABO_1979

Alternate gene names: 110834840

Gene position: 2264585-2263548 (Counterclockwise)

Preceding gene: 110834841

Following gene: 110834839

Centisome position: 72.58

GC content: 51.35

Gene sequence:

>1038_bases
GTGATGCCCTTCATCAATGAGCAGTTAGCAGCGAATCATGAGATAGCGGATCGAAAGCGAGATGCTGACCATTCTGGGAG
AATGTCCCGATTTACCTTTGATCCGGGCACGGTAATGGCAACATTACGTAAACGGATTGTTGGCCAGCCTCATGTGCTAG
ATCGTATCGACAATATGCTGCGAGTCCTCAAGGCGGATATCGCCCCGGATGACCGACCTCTGGCGGTGCAACTGTTTGTA
GGCCCCACCGGGGTCGGTAAGACTGAAACGGTGCGTTTGCTGGCGGAGGCTATTCACGGTAGCGCTGATGCTGTGTGCCG
GATAGATATGAGCACTTTGGCCCAGGAGCATTATGCCGCTTCATTAACTGGAGCGCCGCCAGGGTATGTGGGTAGCAAAG
AAGGCCAAACTCTATTTGATGTAGAGGCTATTCAGGGCAGCTTTAGCAAACCCGGTATTGTGTTGTTTGATGAACTGGAA
AAAGCCAGCCCTGAAGTGGTCAGAGCATTGCTCAATGTGCTGGATCGAGGTCGACTCAGACTGAGCGCCGGCAACCGTGA
AATCAACTTCCGCAATAGCCTAATTTTCATGACCAGTAACGCCGGGGCATCACAAGTGTCGGCCTTGAGAGGGCGGCAAC
AGAAACGCTGGTGGTACTGGAAGAATTCGAGCCGCCAGATAGAAGCGGTGGTGGATCAGGCATTGCAGCAACATTTTGAC
CCGGAATTTCTTAATCGTATTGATAGCATCATTACGTTTAATGCTCTGGATCAGGATATTAGTGAGCAATTGTTGGATAT
CGAGTTGGAAAAGCTCAATCGACGGTTACTAAGACGTAAAGTGCTGCTGAGCTTAGGTGATGGGACACGGCGTTATCTAT
GCAACACCATGGATGCCCGTTATGGGGCCAGGGAAATGGCGCGCCGGGTTCGCAATGAGCTTTCTCCGCCGCTGGCCAGA
GTGATGTTGGATTACCCTCAAGCGGATACCTTCGAGGCACAGATGCAAGCAGGAAAGCTCATCGTTATTCCTGTTTGA

Upstream 100 bases:

>100_bases
CGCAGGAGAACGTAGAAGCCATGACACGTAGCACAATAGGGGTGGCAGAGTGTCCGGTGGGTGAATTGCCTGTGGATGGT
AAAGAGAGAACGGCGGGTGA

Downstream 100 bases:

>100_bases
TTGAGTCTCGAGAGATTAACTAAACCGTCAGCCTGCTGGCGGTTTTACCGCTATCGGTGAGGTGCCGCTTGCCGTACGGA
CAGCCTTGGTGACGGCCCTT

Product: chaperone-associated ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MMPFINEQLAANHEIADRKRDADHSGRMSRFTFDPGTVMATLRKRIVGQPHVLDRIDNMLRVLKADIAPDDRPLAVQLFV
GPTGVGKTETVRLLAEAIHGSADAVCRIDMSTLAQEHYAASLTGAPPGYVGSKEGQTLFDVEAIQGSFSKPGIVLFDELE
KASPEVVRALLNVLDRGRLRLSAGNREINFRNSLIFMTSNAGASQVSALRGRQQKRWWYWKNSSRQIEAVVDQALQQHFD
PEFLNRIDSIITFNALDQDISEQLLDIELEKLNRRLLRRKVLLSLGDGTRRYLCNTMDARYGAREMARRVRNELSPPLAR
VMLDYPQADTFEAQMQAGKLIVIPV

Sequences:

>Translated_345_residues
MMPFINEQLAANHEIADRKRDADHSGRMSRFTFDPGTVMATLRKRIVGQPHVLDRIDNMLRVLKADIAPDDRPLAVQLFV
GPTGVGKTETVRLLAEAIHGSADAVCRIDMSTLAQEHYAASLTGAPPGYVGSKEGQTLFDVEAIQGSFSKPGIVLFDELE
KASPEVVRALLNVLDRGRLRLSAGNREINFRNSLIFMTSNAGASQVSALRGRQQKRWWYWKNSSRQIEAVVDQALQQHFD
PEFLNRIDSIITFNALDQDISEQLLDIELEKLNRRLLRRKVLLSLGDGTRRYLCNTMDARYGAREMARRVRNELSPPLAR
VMLDYPQADTFEAQMQAGKLIVIPV
>Mature_345_residues
MMPFINEQLAANHEIADRKRDADHSGRMSRFTFDPGTVMATLRKRIVGQPHVLDRIDNMLRVLKADIAPDDRPLAVQLFV
GPTGVGKTETVRLLAEAIHGSADAVCRIDMSTLAQEHYAASLTGAPPGYVGSKEGQTLFDVEAIQGSFSKPGIVLFDELE
KASPEVVRALLNVLDRGRLRLSAGNREINFRNSLIFMTSNAGASQVSALRGRQQKRWWYWKNSSRQIEAVVDQALQQHFD
PEFLNRIDSIITFNALDQDISEQLLDIELEKLNRRLLRRKVLLSLGDGTRRYLCNTMDARYGAREMARRVRNELSPPLAR
VMLDYPQADTFEAQMQAGKLIVIPV

Specific function: Unlikely to encode a regulatory protein. Has ATPase activity. AmiB and AmiS may act jointly into a two component ABC transporter system [H]

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=307, Percent_Identity=30.6188925081433, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1788943, Length=284, Percent_Identity=36.9718309859155, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1787109, Length=284, Percent_Identity=33.8028169014084, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320464, Length=310, Percent_Identity=35.1612903225806, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323002, Length=308, Percent_Identity=31.4935064935065, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR001270
- InterPro:   IPR019489 [H]

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 38797; Mature: 38797

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPFINEQLAANHEIADRKRDADHSGRMSRFTFDPGTVMATLRKRIVGQPHVLDRIDNML
CCCCCCHHHHCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
RVLKADIAPDDRPLAVQLFVGPTGVGKTETVRLLAEAIHGSADAVCRIDMSTLAQEHYAA
HHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
SLTGAPPGYVGSKEGQTLFDVEAIQGSFSKPGIVLFDELEKASPEVVRALLNVLDRGRLR
CCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCEE
LSAGNREINFRNSLIFMTSNAGASQVSALRGRQQKRWWYWKNSSRQIEAVVDQALQQHFD
EECCCEEEEECCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCC
PEFLNRIDSIITFNALDQDISEQLLDIELEKLNRRLLRRKVLLSLGDGTRRYLCNTMDAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YGAREMARRVRNELSPPLARVMLDYPQADTFEAQMQAGKLIVIPV
CCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHCCCCEEEEEC
>Mature Secondary Structure
MMPFINEQLAANHEIADRKRDADHSGRMSRFTFDPGTVMATLRKRIVGQPHVLDRIDNML
CCCCCCHHHHCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
RVLKADIAPDDRPLAVQLFVGPTGVGKTETVRLLAEAIHGSADAVCRIDMSTLAQEHYAA
HHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
SLTGAPPGYVGSKEGQTLFDVEAIQGSFSKPGIVLFDELEKASPEVVRALLNVLDRGRLR
CCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCEE
LSAGNREINFRNSLIFMTSNAGASQVSALRGRQQKRWWYWKNSSRQIEAVVDQALQQHFD
EECCCEEEEECCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCC
PEFLNRIDSIITFNALDQDISEQLLDIELEKLNRRLLRRKVLLSLGDGTRRYLCNTMDAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YGAREMARRVRNELSPPLARVMLDYPQADTFEAQMQAGKLIVIPV
CCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7642533; 10984043; 7539417 [H]