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The map label for this gene is amiE

Identifier: 110834841

GI number: 110834841

Start: 2264582

End: 2265628

Strand: Reverse

Name: amiE

Synonym: ABO_1980

Alternate gene names: 110834841

Gene position: 2265628-2264582 (Counterclockwise)

Preceding gene: 110834842

Following gene: 110834840

Centisome position: 72.61

GC content: 49.38

Gene sequence:

>1047_bases
ATGCGACACGGCGATATTTCCAGCAGTAACGATACCGTGGGTGTTGCGGTAGTGAATTACAAAATGCCGCGCCTGCATAG
CAAAGCTGAAGTGTTAGATAATGCCCGTAACATTGCGGAAATGATTAAGGGCATGAAAAAGGGACTGCCGGGGATGGATT
TGGTGGTTTTTCCGGAATACAGCACTATGGGCATTATGTATGACCCGGATGAAATGATGGCCACAGCGGCTACCATTCCC
GGTGATGAGACCGCTATTTTCTCCGCTGCCTGTCGTGAAGCTAATACCTGGGGTGTGTTTTCGCTGACGGGCGAACAACA
TGAAGAGCACCCTGCGAAAGCCCCTTATAACACGCTGGTGTTAATCAATAATGAAGGGGAAATTGTTCAGAAATACCGTA
AGTGTATTCCGTGGTGTCCTATTGAGGGTTGGTATCCTGGTGATACGACCTATGTAACGGAAGGTCCCAAGGGAATGAAG
ATCAGCCTCATCATTTGTGATGACGGTAACTACCCGGAAATCTGGCGTGATTGTGCCATGAAAGGTGCTGAGTTGATTGT
TCGTTGCCAGGGCTATATGTATCCGGCGAAGGAGCAGCAGGTGATGATGGCCAAGTCCATGGCCTGGGCGAATAACTGCT
ATGTGGCTGTAGCTAACGCCAGTGGTTTTGATGGCGTGTATTCGTATTTTGGGCACTCGGCACTCATCGGGTTTGATGGT
CGTACTCTGGGTGAATGTGGTGAAGAGGATATGGGTATTCAGTATGCCCAGTTATCCGTCAGTCAAATCCGTGATGCTCG
TGCTAATGATCAGTCTCAAAATCATCTGTTTAAGTTGCTGCACCGTGGTTATACCGGCATGCATGCTTCTGGTGATGGAG
ACAAGGGTATTGCGGACTGTCCTTTTGACTTTTATCGCACCTGGGTAATGGATGCTCAAAAGGCGCAGGAGAACGTAGAA
GCCATGACACGTAGCACAATAGGGGTGGCAGAGTGTCCGGTGGGTGAATTGCCTGTGGATGGTAAAGAGAGAACGGCGGG
TGAGTGA

Upstream 100 bases:

>100_bases
AAGAAAGCGGTGAGAGCATGAATCAGGAAAAAATAGCGGCATATCTTGCGGTGTGATGTGCCTGTTTGCCGATGAATTTT
AAGCAGTAAAGGAGAGAGTA

Downstream 100 bases:

>100_bases
TGCCCTTCATCAATGAGCAGTTAGCAGCGAATCATGAGATAGCGGATCGAAAGCGAGATGCTGACCATTCTGGGAGAATG
TCCCGATTTACCTTTGATCC

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRHGDISSSNDTVGVAVVNYKMPRLHSKAEVLDNARNIAEMIKGMKKGLPGMDLVVFPEYSTMGIMYDPDEMMATAATIP
GDETAIFSAACREANTWGVFSLTGEQHEEHPAKAPYNTLVLINNEGEIVQKYRKCIPWCPIEGWYPGDTTYVTEGPKGMK
ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQVMMAKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG
RTLGECGEEDMGIQYAQLSVSQIRDARANDQSQNHLFKLLHRGYTGMHASGDGDKGIADCPFDFYRTWVMDAQKAQENVE
AMTRSTIGVAECPVGELPVDGKERTAGE

Sequences:

>Translated_348_residues
MRHGDISSSNDTVGVAVVNYKMPRLHSKAEVLDNARNIAEMIKGMKKGLPGMDLVVFPEYSTMGIMYDPDEMMATAATIP
GDETAIFSAACREANTWGVFSLTGEQHEEHPAKAPYNTLVLINNEGEIVQKYRKCIPWCPIEGWYPGDTTYVTEGPKGMK
ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQVMMAKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG
RTLGECGEEDMGIQYAQLSVSQIRDARANDQSQNHLFKLLHRGYTGMHASGDGDKGIADCPFDFYRTWVMDAQKAQENVE
AMTRSTIGVAECPVGELPVDGKERTAGE
>Mature_348_residues
MRHGDISSSNDTVGVAVVNYKMPRLHSKAEVLDNARNIAEMIKGMKKGLPGMDLVVFPEYSTMGIMYDPDEMMATAATIP
GDETAIFSAACREANTWGVFSLTGEQHEEHPAKAPYNTLVLINNEGEIVQKYRKCIPWCPIEGWYPGDTTYVTEGPKGMK
ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQVMMAKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG
RTLGECGEEDMGIQYAQLSVSQIRDARANDQSQNHLFKLLHRGYTGMHASGDGDKGIADCPFDFYRTWVMDAQKAQENVE
AMTRSTIGVAECPVGELPVDGKERTAGE

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIE_ALCBS (Q0VN20)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693700.1
- STRING:   Q0VN20
- GeneID:   4211291
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_1980
- NMPDR:   fig|393595.12.peg.1981
- eggNOG:   COG0388
- HOGENOM:   HBG287568
- OMA:   QNHLFKL
- ProtClustDB:   PRK13286
- BioCyc:   ABOR393595:ABO_1980-MONOMER
- HAMAP:   MF_01242
- InterPro:   IPR003010
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: =3.5.1.4

Molecular weight: Translated: 38360; Mature: 38360

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50263 CN_HYDROLASE

Important sites: ACT_SITE 59-59 ACT_SITE 134-134 ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHGDISSSNDTVGVAVVNYKMPRLHSKAEVLDNARNIAEMIKGMKKGLPGMDLVVFPEY
CCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STMGIMYDPDEMMATAATIPGDETAIFSAACREANTWGVFSLTGEQHEEHPAKAPYNTLV
CCEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEE
LINNEGEIVQKYRKCIPWCPIEGWYPGDTTYVTEGPKGMKISLIICDDGNYPEIWRDCAM
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHH
KGAELIVRCQGYMYPAKEQQVMMAKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCC
RTLGECGEEDMGIQYAQLSVSQIRDARANDQSQNHLFKLLHRGYTGMHASGDGDKGIADC
CCHHHCCCHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PFDFYRTWVMDAQKAQENVEAMTRSTIGVAECPVGELPVDGKERTAGE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRHGDISSSNDTVGVAVVNYKMPRLHSKAEVLDNARNIAEMIKGMKKGLPGMDLVVFPEY
CCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STMGIMYDPDEMMATAATIPGDETAIFSAACREANTWGVFSLTGEQHEEHPAKAPYNTLV
CCEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEE
LINNEGEIVQKYRKCIPWCPIEGWYPGDTTYVTEGPKGMKISLIICDDGNYPEIWRDCAM
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHH
KGAELIVRCQGYMYPAKEQQVMMAKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCC
RTLGECGEEDMGIQYAQLSVSQIRDARANDQSQNHLFKLLHRGYTGMHASGDGDKGIADC
CCHHHCCCHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PFDFYRTWVMDAQKAQENVEAMTRSTIGVAECPVGELPVDGKERTAGE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA