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The map label for this gene is lipA

Identifier: 110834825

GI number: 110834825

Start: 2243677

End: 2244654

Strand: Direct

Name: lipA

Synonym: ABO_1964

Alternate gene names: 110834825

Gene position: 2243677-2244654 (Clockwise)

Preceding gene: 110834824

Following gene: 110834826

Centisome position: 71.91

GC content: 56.65

Gene sequence:

>978_bases
ATGAGCGAACAGGCCCAAGCCAAACGCAAAGTGCAAATCGGTGACAAACTACGTGGCGCCGACAAGGTCCGCACCATCCC
CATGGTTAATGAAGAAACCGGTTACCAACGTAAGCCGGACTGGATTCGTGTGCGTGTTCCTGCCAATGGCGAAATTCAGC
GTATCAAGTCCCTGCTACGCAAACAGAAACTGCACACCGTTTGCGAAGAGGCGGCCTGCCCCAACTTGCCGGAATGTTTT
GGCGGCGGCACCGCCACCTTCATGATCATGGGCGACATCTGTACCCGTCGTTGCGCTTTCTGCGATGTGGGTTTCGGCCG
GCCCAATGCGTTGGATGCGCAAGAGCCGTTGCACTTGGCGGAGTCTGTGGAAAACTTGGGCCTGAATTATGTGGTGATTA
CCTCCGTGGACCGGGATGATCTAGCCGACGGCGGCGCGGAACACTTTGCCGAGTGCATCCGCCAGGTGCGAGCGCTTACC
CCAGAGACCCGCATCGAAATCCTCACCCCAGACTTTCGCCCCTGCCTGGACACCGCCGTGGAGATTCTCGCTGAAACCGC
GCCGGACGTGTTTAACCACAATATTGAAACCGTTCCGGAGCTATATAAGCACATCCGCCCCGGCGCCCGTTATCAACACT
CCCTGGATTTGCTTAAACGCTACAAGGCTCTGCGCCCGGATGTGAGCACCAAAAGCGGCATCATGGTGGGGTTGGGTGAA
ACGTTCGAGCAGGTCATCAATACCATCAAAGATCTGCGAACCCATGATGTGGACATGATCACCATTGGCCAATATCTGCA
GCCCAGCAAGCACCACGCTCCGGTGGATCGCTTCGTACACCCGGACGAATTCCGGGAGTATGCCCGCGTGGCCAACGAAC
TAGGCTTCACCTCGGTTGCCTCCGGGCCCATGGTGCGCAGTTCCTACCATGCTGATTTACAGCATAAAGGGGTGGATGTT
GGCCTATACAAACCCTAA

Upstream 100 bases:

>100_bases
GATCCTGGCAGTGCTCTGACTCAACAGCCAGAGCGGCTGTAACCACAGACACCGCTGCCTGCCCCCCCTTCCGGCAGCAC
TCGACGGCAATGGAAACCCC

Downstream 100 bases:

>100_bases
TTCAAAGGCCGGGCTTACTGGCACCACCGATTAAATGTACGTTTCGTACTAGCGGCCCACTGACTCGGCTCGGGCTTTGC
ATTACGCTAGCGGTCTATGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSEQAQAKRKVQIGDKLRGADKVRTIPMVNEETGYQRKPDWIRVRVPANGEIQRIKSLLRKQKLHTVCEEAACPNLPECF
GGGTATFMIMGDICTRRCAFCDVGFGRPNALDAQEPLHLAESVENLGLNYVVITSVDRDDLADGGAEHFAECIRQVRALT
PETRIEILTPDFRPCLDTAVEILAETAPDVFNHNIETVPELYKHIRPGARYQHSLDLLKRYKALRPDVSTKSGIMVGLGE
TFEQVINTIKDLRTHDVDMITIGQYLQPSKHHAPVDRFVHPDEFREYARVANELGFTSVASGPMVRSSYHADLQHKGVDV
GLYKP

Sequences:

>Translated_325_residues
MSEQAQAKRKVQIGDKLRGADKVRTIPMVNEETGYQRKPDWIRVRVPANGEIQRIKSLLRKQKLHTVCEEAACPNLPECF
GGGTATFMIMGDICTRRCAFCDVGFGRPNALDAQEPLHLAESVENLGLNYVVITSVDRDDLADGGAEHFAECIRQVRALT
PETRIEILTPDFRPCLDTAVEILAETAPDVFNHNIETVPELYKHIRPGARYQHSLDLLKRYKALRPDVSTKSGIMVGLGE
TFEQVINTIKDLRTHDVDMITIGQYLQPSKHHAPVDRFVHPDEFREYARVANELGFTSVASGPMVRSSYHADLQHKGVDV
GLYKP
>Mature_324_residues
SEQAQAKRKVQIGDKLRGADKVRTIPMVNEETGYQRKPDWIRVRVPANGEIQRIKSLLRKQKLHTVCEEAACPNLPECFG
GGTATFMIMGDICTRRCAFCDVGFGRPNALDAQEPLHLAESVENLGLNYVVITSVDRDDLADGGAEHFAECIRQVRALTP
ETRIEILTPDFRPCLDTAVEILAETAPDVFNHNIETVPELYKHIRPGARYQHSLDLLKRYKALRPDVSTKSGIMVGLGET
FEQVINTIKDLRTHDVDMITIGQYLQPSKHHAPVDRFVHPDEFREYARVANELGFTSVASGPMVRSSYHADLQHKGVDVG
LYKP

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=49.645390070922, Blast_Score=285, Evalue=3e-77,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=49.3927125506073, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI1786846, Length=311, Percent_Identity=58.1993569131833, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32564533, Length=316, Percent_Identity=42.4050632911392, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=46.2897526501767, Blast_Score=262, Evalue=6e-71,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=47.8723404255319, Blast_Score=274, Evalue=7e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_ALCBS (Q0VN36)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693684.1
- STRING:   Q0VN36
- GeneID:   4211549
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_1964
- NMPDR:   fig|393595.12.peg.1965
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q0VN36
- ProtClustDB:   PRK05481
- BioCyc:   ABOR393595:ABO_1964-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36458; Mature: 36327

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQAQAKRKVQIGDKLRGADKVRTIPMVNEETGYQRKPDWIRVRVPANGEIQRIKSLLR
CCCHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
KQKLHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCAFCDVGFGRPNALDAQEPLHLA
HHHHHHHHHHHCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ESVENLGLNYVVITSVDRDDLADGGAEHFAECIRQVRALTPETRIEILTPDFRPCLDTAV
HHHHHCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
EILAETAPDVFNHNIETVPELYKHIRPGARYQHSLDLLKRYKALRPDVSTKSGIMVGLGE
HHHHHHCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECHH
TFEQVINTIKDLRTHDVDMITIGQYLQPSKHHAPVDRFVHPDEFREYARVANELGFTSVA
HHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCHHHC
SGPMVRSSYHADLQHKGVDVGLYKP
CCCCHHHHHHCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEQAQAKRKVQIGDKLRGADKVRTIPMVNEETGYQRKPDWIRVRVPANGEIQRIKSLLR
CCHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
KQKLHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCAFCDVGFGRPNALDAQEPLHLA
HHHHHHHHHHHCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ESVENLGLNYVVITSVDRDDLADGGAEHFAECIRQVRALTPETRIEILTPDFRPCLDTAV
HHHHHCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
EILAETAPDVFNHNIETVPELYKHIRPGARYQHSLDLLKRYKALRPDVSTKSGIMVGLGE
HHHHHHCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECHH
TFEQVINTIKDLRTHDVDMITIGQYLQPSKHHAPVDRFVHPDEFREYARVANELGFTSVA
HHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCHHHC
SGPMVRSSYHADLQHKGVDVGLYKP
CCCCHHHHHHCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA