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The map label for this gene is lipB

Identifier: 110834824

GI number: 110834824

Start: 2242878

End: 2243618

Strand: Direct

Name: lipB

Synonym: ABO_1963

Alternate gene names: 110834824

Gene position: 2242878-2243618 (Clockwise)

Preceding gene: 110834823

Following gene: 110834825

Centisome position: 71.88

GC content: 57.76

Gene sequence:

>741_bases
ATGCATAGCTGGCCGTATACTTCCCCCATGGACGCACTGATTCGCTATTTCCCACAAGTCGATTATTCCTCTTGCTGGCA
CGCCATGCGCGATCTCACCGATCACCGTGACGCGCAGCAGAGCGACGAGTTTTGGGTACTCGAACACCCTCCCATCTTCA
CGCTGGGCCAAGCCGGCAAGCCCGAACATGTGCTCAATGCCGGTGAGATTCCAGTAGTGAACAGCGACCGTGGTGGGCAA
GTGACTTATCACGGCCCCGGCCAGACAGTGGTCTACCTGATGCTGGACATCAAACGGCTAGGGCTGGGTAGCCGCGGGCT
GGTCTCCGCCATTGAACAAGGCATCATCGACTTTCTTGCAAGTCTGGGCATTACCGCCGTTAATCGAGATGACGCACCCG
GAGTCTATGTTCAAGGCGCCAAAATTGCGTCTCTGGGCCTGCGTATCCGCCGTGGCGCCACCTACCACGGGCTAGCCATC
AACCGGGATATGGACCTTTCCCCCTGGCATCGTATTAACCCCTGCGGACACGTGGATCAACCCATGACCACACTGAAAGC
TCAAGGGGTGGATCTGGACCGCCACAGCATGGAACAACAGCTGGTATCCTTTTTAGCCAAACGTCTTGGGCTAACGCCCC
GGACAGCGGCCCTTCCGGACTGGTACAATACCCGCCAAGAGAATGTCACCACTGGCGGCGATCCTGGCAGTGCTCTGACT
CAACAGCCAGAGCGGCTGTAA

Upstream 100 bases:

>100_bases
TTTTAGGGAACGTCAACCGCGACCCTGTAGAAAATGCGTACATCCGCGGATTTTGATTCTTCTCTATCCAATGTGCATCG
CGCAAAGCGGCTAGCCTGTC

Downstream 100 bases:

>100_bases
CCACAGACACCGCTGCCTGCCCCCCCTTCCGGCAGCACTCGACGGCAATGGAAACCCCATGAGCGAACAGGCCCAAGCCA
AACGCAAAGTGCAAATCGGT

Product: lipoyl-[acyl-carrier protein]-protein-n-lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MHSWPYTSPMDALIRYFPQVDYSSCWHAMRDLTDHRDAQQSDEFWVLEHPPIFTLGQAGKPEHVLNAGEIPVVNSDRGGQ
VTYHGPGQTVVYLMLDIKRLGLGSRGLVSAIEQGIIDFLASLGITAVNRDDAPGVYVQGAKIASLGLRIRRGATYHGLAI
NRDMDLSPWHRINPCGHVDQPMTTLKAQGVDLDRHSMEQQLVSFLAKRLGLTPRTAALPDWYNTRQENVTTGGDPGSALT
QQPERL

Sequences:

>Translated_246_residues
MHSWPYTSPMDALIRYFPQVDYSSCWHAMRDLTDHRDAQQSDEFWVLEHPPIFTLGQAGKPEHVLNAGEIPVVNSDRGGQ
VTYHGPGQTVVYLMLDIKRLGLGSRGLVSAIEQGIIDFLASLGITAVNRDDAPGVYVQGAKIASLGLRIRRGATYHGLAI
NRDMDLSPWHRINPCGHVDQPMTTLKAQGVDLDRHSMEQQLVSFLAKRLGLTPRTAALPDWYNTRQENVTTGGDPGSALT
QQPERL
>Mature_246_residues
MHSWPYTSPMDALIRYFPQVDYSSCWHAMRDLTDHRDAQQSDEFWVLEHPPIFTLGQAGKPEHVLNAGEIPVVNSDRGGQ
VTYHGPGQTVVYLMLDIKRLGLGSRGLVSAIEQGIIDFLASLGITAVNRDDAPGVYVQGAKIASLGLRIRRGATYHGLAI
NRDMDLSPWHRINPCGHVDQPMTTLKAQGVDLDRHSMEQQLVSFLAKRLGLTPRTAALPDWYNTRQENVTTGGDPGSALT
QQPERL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=173, Percent_Identity=56.0693641618497, Blast_Score=202, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323268, Length=171, Percent_Identity=34.5029239766082, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355483, Length=153, Percent_Identity=30.718954248366, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_ALCBS (Q0VN37)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693683.1
- ProteinModelPortal:   Q0VN37
- STRING:   Q0VN37
- GeneID:   4211550
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_1963
- NMPDR:   fig|393595.12.peg.1964
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   VEWINEQ
- PhylomeDB:   Q0VN37
- BioCyc:   ABOR393595:ABO_1963-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 27262; Mature: 27262

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSWPYTSPMDALIRYFPQVDYSSCWHAMRDLTDHRDAQQSDEFWVLEHPPIFTLGQAGK
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
PEHVLNAGEIPVVNSDRGGQVTYHGPGQTVVYLMLDIKRLGLGSRGLVSAIEQGIIDFLA
CHHHCCCCCCCEEECCCCCEEEEECCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SLGITAVNRDDAPGVYVQGAKIASLGLRIRRGATYHGLAINRDMDLSPWHRINPCGHVDQ
HCCCEEECCCCCCCEEEECCEEHHHCEEHCCCCEEECEEECCCCCCCCCCCCCCCCCCCC
PMTTLKAQGVDLDRHSMEQQLVSFLAKRLGLTPRTAALPDWYNTRQENVTTGGDPGSALT
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHC
QQPERL
CCCCCC
>Mature Secondary Structure
MHSWPYTSPMDALIRYFPQVDYSSCWHAMRDLTDHRDAQQSDEFWVLEHPPIFTLGQAGK
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
PEHVLNAGEIPVVNSDRGGQVTYHGPGQTVVYLMLDIKRLGLGSRGLVSAIEQGIIDFLA
CHHHCCCCCCCEEECCCCCEEEEECCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SLGITAVNRDDAPGVYVQGAKIASLGLRIRRGATYHGLAINRDMDLSPWHRINPCGHVDQ
HCCCEEECCCCCCCEEEECCEEHHHCEEHCCCCEEECEEECCCCCCCCCCCCCCCCCCCC
PMTTLKAQGVDLDRHSMEQQLVSFLAKRLGLTPRTAALPDWYNTRQENVTTGGDPGSALT
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHC
QQPERL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA