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The map label for this gene is dacB [H]

Identifier: 110834826

GI number: 110834826

Start: 2244808

End: 2246316

Strand: Direct

Name: dacB [H]

Synonym: ABO_1965

Alternate gene names: 110834826

Gene position: 2244808-2246316 (Clockwise)

Preceding gene: 110834825

Following gene: 110834827

Centisome position: 71.95

GC content: 56.86

Gene sequence:

>1509_bases
ATGCCGAGAAGCGCAGTCCGTAAACAGCCTAACATCGTTCTTACTTCAACCCTTCTCGGCATTTTTCTGCTGCTCGGCAC
CTTGCTTGCCGGCTCCGCACAAGCGGATATTCCGGCGCCAGTCATGGCCCCAGTATTGGAAGTCGCGGAAAGCCTGGAGC
TTGCCGAAACCGACCTGGCAGTAGCTTCCATCCCCCTGGATGGACCCGGAAAAGCCCGTTTCATGAATGCGGACATACCC
CTAAACCCCGGCTCCATCATGAAGGTCATTACCACCTACGCGGCGCTAGAATTACTGGGTCCCACGTTTCAATGGCACAC
TCGCCTGTACACCGACGGTACCATTGAGGGGGAAACCCTCAGCGGTAACCTGTATTTTGTTGGCAGCGGGGACCCTAAAC
TCACCGAAGAGCGGTTATGGCTACTGCTGCGGGAGTTGCGTGCCATGGGTATCACCCACATCACCGGTGACCTGATTTTG
GATGGCAGCGTGTTCAACCTGCCCAATGGCATTACCGCCTTTAATGATGACGGTGACAACCCCAACGCACCCTTCCTGGT
GGAACCCAACGGCTTGCTCACCAACTTGAATGTGTTGCGCATCCGGGCCCGAGCAGACAACCGCGGCATTCATACTTGGA
CCGAACCGGCCCTCAATGGCATCCACATCAACAACCAACTGGTACTGCGCGAGTACGGCCATTGCCCTCGCCGCTATCAA
TTTGACTATGAACCTACCACCCGTGATGACGGCTCAATAAGCATCACGGTCACCGGCGTGCTGCCCGTAGGGTGTAGCAC
CAGCAGCTATCTGTCTCTGCTAAGCCAGCCCGCCTATACCGGTGCCCTACTCTCTAGCCTGTGGCAGCAGCTTGGCGGTA
CCCTTTCAGGAACCATCCGCGAAGGCCTGCGCCCTCGAGCAGACACAGAACTACTCGTCACCACCTCATCGCGTGATTTG
GTCACTATGGTCCGCGATATTAATAAGTGGAGTAATAATGTGATGGTGCGCCAGGTCTTCCTGACGTTGGGCGCCAAGCA
CCGTCAGCCCGGCGATAAAGACGATCTTGCCGCCGCAGAGCGTGAAATTCGCGCCTGGCTAGCCCATAAAGGTATCGACA
GCCGGAGTATGGTCTTTGAAAATGGGGCCGGCTTGTCACGCATTGAGCGCATCACCGCCCGTCAAATGGCCCAACTACTG
CAACAGGCCTGGGAAAGCCGCTTTGCCGCAGAACTAATTGCCTCCCTGCCACTGGTGGCCATGGATGGCACAATGCGTCG
CAGGCTGGGTAACGCTAACATGGCTGGCATGGGCCACATTAAAACCGGCTCTTTGCGTAATGTGCGCTCCATCGCAGGGT
TCACCCGCGATGAAGACAACACCACCTGGGCAGTGGTGGCCATGGTGAACAACCCTCGCGCGTGGCAAAGCGAGCCGGTA
CTTGATGCGTTCATCGAACAAGTGCACCTATCCACGCGCCCTAAAAGCATAAACACCGCCCGGCAATAA

Upstream 100 bases:

>100_bases
GGCCCACTGACTCGGCTCGGGCTTTGCATTACGCTAGCGGTCTATGTAACACGCTTACCCCAGCAGTTGTTGCCGTTACG
ATTTTGAAGGTCCCCTTGTC

Downstream 100 bases:

>100_bases
ATCGTCCCCCCGCAGGGTCGATGGCCCTGCTTTATTCTACTGTGCGTTTTCCCTATGCTGAGCACATAAGGAGCTTTCAG
AAGCCCCGTATCTGCCCCCC

Product: D-alanyl-D-alanine-carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPRSAVRKQPNIVLTSTLLGIFLLLGTLLAGSAQADIPAPVMAPVLEVAESLELAETDLAVASIPLDGPGKARFMNADIP
LNPGSIMKVITTYAALELLGPTFQWHTRLYTDGTIEGETLSGNLYFVGSGDPKLTEERLWLLLRELRAMGITHITGDLIL
DGSVFNLPNGITAFNDDGDNPNAPFLVEPNGLLTNLNVLRIRARADNRGIHTWTEPALNGIHINNQLVLREYGHCPRRYQ
FDYEPTTRDDGSISITVTGVLPVGCSTSSYLSLLSQPAYTGALLSSLWQQLGGTLSGTIREGLRPRADTELLVTTSSRDL
VTMVRDINKWSNNVMVRQVFLTLGAKHRQPGDKDDLAAAEREIRAWLAHKGIDSRSMVFENGAGLSRIERITARQMAQLL
QQAWESRFAAELIASLPLVAMDGTMRRRLGNANMAGMGHIKTGSLRNVRSIAGFTRDEDNTTWAVVAMVNNPRAWQSEPV
LDAFIEQVHLSTRPKSINTARQ

Sequences:

>Translated_502_residues
MPRSAVRKQPNIVLTSTLLGIFLLLGTLLAGSAQADIPAPVMAPVLEVAESLELAETDLAVASIPLDGPGKARFMNADIP
LNPGSIMKVITTYAALELLGPTFQWHTRLYTDGTIEGETLSGNLYFVGSGDPKLTEERLWLLLRELRAMGITHITGDLIL
DGSVFNLPNGITAFNDDGDNPNAPFLVEPNGLLTNLNVLRIRARADNRGIHTWTEPALNGIHINNQLVLREYGHCPRRYQ
FDYEPTTRDDGSISITVTGVLPVGCSTSSYLSLLSQPAYTGALLSSLWQQLGGTLSGTIREGLRPRADTELLVTTSSRDL
VTMVRDINKWSNNVMVRQVFLTLGAKHRQPGDKDDLAAAEREIRAWLAHKGIDSRSMVFENGAGLSRIERITARQMAQLL
QQAWESRFAAELIASLPLVAMDGTMRRRLGNANMAGMGHIKTGSLRNVRSIAGFTRDEDNTTWAVVAMVNNPRAWQSEPV
LDAFIEQVHLSTRPKSINTARQ
>Mature_501_residues
PRSAVRKQPNIVLTSTLLGIFLLLGTLLAGSAQADIPAPVMAPVLEVAESLELAETDLAVASIPLDGPGKARFMNADIPL
NPGSIMKVITTYAALELLGPTFQWHTRLYTDGTIEGETLSGNLYFVGSGDPKLTEERLWLLLRELRAMGITHITGDLILD
GSVFNLPNGITAFNDDGDNPNAPFLVEPNGLLTNLNVLRIRARADNRGIHTWTEPALNGIHINNQLVLREYGHCPRRYQF
DYEPTTRDDGSISITVTGVLPVGCSTSSYLSLLSQPAYTGALLSSLWQQLGGTLSGTIREGLRPRADTELLVTTSSRDLV
TMVRDINKWSNNVMVRQVFLTLGAKHRQPGDKDDLAAAEREIRAWLAHKGIDSRSMVFENGAGLSRIERITARQMAQLLQ
QAWESRFAAELIASLPLVAMDGTMRRRLGNANMAGMGHIKTGSLRNVRSIAGFTRDEDNTTWAVVAMVNNPRAWQSEPVL
DAFIEQVHLSTRPKSINTARQ

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=382, Percent_Identity=28.5340314136126, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 55021; Mature: 54889

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSAVRKQPNIVLTSTLLGIFLLLGTLLAGSAQADIPAPVMAPVLEVAESLELAETDLA
CCCHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEE
VASIPLDGPGKARFMNADIPLNPGSIMKVITTYAALELLGPTFQWHTRLYTDGTIEGETL
EEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEECCCEE
SGNLYFVGSGDPKLTEERLWLLLRELRAMGITHITGDLILDGSVFNLPNGITAFNDDGDN
ECEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCEEECCCEEECCCCCEEECCCCCC
PNAPFLVEPNGLLTNLNVLRIRARADNRGIHTWTEPALNGIHINNQLVLREYGHCPRRYQ
CCCCEEECCCCCEECCEEEEEEEECCCCCCCCCCCCCCCCEEECCEEEEHHHCCCCCCEE
FDYEPTTRDDGSISITVTGVLPVGCSTSSYLSLLSQPAYTGALLSSLWQQLGGTLSGTIR
CCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHH
EGLRPRADTELLVTTSSRDLVTMVRDINKWSNNVMVRQVFLTLGAKHRQPGDKDDLAAAE
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
REIRAWLAHKGIDSRSMVFENGAGLSRIERITARQMAQLLQQAWESRFAAELIASLPLVA
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
MDGTMRRRLGNANMAGMGHIKTGSLRNVRSIAGFTRDEDNTTWAVVAMVNNPRAWQSEPV
ECCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCH
LDAFIEQVHLSTRPKSINTARQ
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PRSAVRKQPNIVLTSTLLGIFLLLGTLLAGSAQADIPAPVMAPVLEVAESLELAETDLA
CCHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEE
VASIPLDGPGKARFMNADIPLNPGSIMKVITTYAALELLGPTFQWHTRLYTDGTIEGETL
EEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEECCCEE
SGNLYFVGSGDPKLTEERLWLLLRELRAMGITHITGDLILDGSVFNLPNGITAFNDDGDN
ECEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCEEECCCEEECCCCCEEECCCCCC
PNAPFLVEPNGLLTNLNVLRIRARADNRGIHTWTEPALNGIHINNQLVLREYGHCPRRYQ
CCCCEEECCCCCEECCEEEEEEEECCCCCCCCCCCCCCCCEEECCEEEEHHHCCCCCCEE
FDYEPTTRDDGSISITVTGVLPVGCSTSSYLSLLSQPAYTGALLSSLWQQLGGTLSGTIR
CCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHH
EGLRPRADTELLVTTSSRDLVTMVRDINKWSNNVMVRQVFLTLGAKHRQPGDKDDLAAAE
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
REIRAWLAHKGIDSRSMVFENGAGLSRIERITARQMAQLLQQAWESRFAAELIASLPLVA
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
MDGTMRRRLGNANMAGMGHIKTGSLRNVRSIAGFTRDEDNTTWAVVAMVNNPRAWQSEPV
ECCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCH
LDAFIEQVHLSTRPKSINTARQ
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]