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The map label for this gene is ligA

Identifier: 110833810

GI number: 110833810

Start: 1067810

End: 1070143

Strand: Direct

Name: ligA

Synonym: ABO_0949

Alternate gene names: 110833810

Gene position: 1067810-1070143 (Clockwise)

Preceding gene: 110833809

Following gene: 110833811

Centisome position: 34.22

GC content: 57.24

Gene sequence:

>2334_bases
GTGACGAATAACAACGATCCTTCCCTGCAAGCACAATCCCTCCGTGATCAGCTTAACGACTGGTCCTACCGTTACTATGT
TCAAGATGACCCGGCGGTGCCGGATGCGGAGTACGATCGGGTATTTCGCGCACTGAAAGACCTGGAAGCGCAATACCCTG
ATCTAGTTACCGCAGATTCGCCCACCCAGCGGGTGGGTGATGTGCCGTTGGATGCGTTTGAGCAGGTGCAGCATGAAGTG
CCGATGTTGAGCCTGGACAATGCCTTCGATGATGAGGAGCTATGGGCCTTTGATAAGCGTATTCGTGAACGCTTGGATGT
GAGCGAAATCATTGATTACGTGGCCGAGCCCAAGCTCGACGGGTTGGCGGTGTCGTTGCTTTATGAGAACGGTGAGTTGG
TGCGGGCGGCCACCCGCGGCGATGGGCAAACCGGTGAGAATATTACCGTGAATGCCAGAACTATTCGCTCGGTGCCTCTT
AAACTGCGTGGCAATGATGTGCCTAAGCGGTTGGAAGTGCGCGGTGAAGTGGTCATGCCCCACGCTGGCTTTGAGGAGCT
TAATGCTCGCCAGCAAGAGGCTGGGCTAAAGCTTTTTGCTAACCCTCGTAATGCCGCGGCAGGCAGTTTGCGGCAATTGG
ATTCTCGCATCACTGCCAGCCGCCCGCTGGAATTTTATGCCTACTCCATGGCCCAGCTGGAAGGCTGGGAGCATCCGCCT
ACCCATTCGGCCATGCTTGATGCATTACGCGACTGGGGTTTACGCGTAAACCCGGAGATTCGCGTTTGTCACGGGGTAGA
GGCCTTGCTGCGTTTTTATCAGGGCATCTTGGAAAAACGCGAGCATCTGGATTACGACATTGATGGTGTGGTGTACAAGG
TAAACCGCTTCGACTGGCAGGATGACCTGGGCTTTGTTAGCCGGGCGCCGCGCTGGGCCATCGCCCACAAATTTCCCGCC
CAGGAAGAGCTCACCGTGCTGAACGGTGTGGATTGGCAGGTAGGCCGTACCGGGGCGCTGACGCCGGTGGCGCGGTTGGA
ACCGGTGCACGTGGGCGGGGTGATCGTTAGCAATGCCACGCTACACAATATTGATGAAATTCAGCGCTTGGATATTCGCA
TTGGCGATACCGTGGTGGTGTACCGGGCCGGCGATGTGATTCCCAAGGTGGTGCGGGCACTGCCAGAGCGACGTCCGGCA
AATGCGCAAGGCATTGCTTTGCCGTCGTCGTGCCCGGTGTGTGGTAGTGAAATTCTGCGTGGCCATGATCAAGTGGTGGC
GCGCTGTACCGGCGGCTTGATCTGTGGCGATCAACGGCGTGAAGCCATCAAGCATTTTGCCTCCCGCCGGGCCATGGATA
TTGATGGGCTGGGTGACAAGCTAGTGGATGCGCTGGTGGATCAGGAGCTGATTACCACTGTGGCCGATCTGTACCGCTTG
AAAGCCGAGCAGGTGGCGGGCCTGGAGCGCATGGGGGAGAAATCGGCGGATAATCTTATTGCGGCGCTGGAAGCCTCTAA
AACCGTGGGGTTAGGCCGCTTCCTGTTCGCGCTGGGGATCCTACAGATTGGTGAAGAAACCGCCAAGAATCTGGCTGACT
GTTTTGGCGATCTGGATAGCATTCGCCATGCTCCGTTGTTGTTGTTGCTGGCGGTGCCGGATGTGGGTCTGGAAGTGGCC
AAGGCCATCGGTGCTTTCTTTGCCGAAAGTGATAACGAGGCGGTGATCGACGGTTTGTTGGCGCAGGGGGTGAGCCCACA
GGCCAGCGGAGTACCGTCTGCTGCCTTTGTGAAAAGTCTGACGTTGGCGCAATTGTTAAAGTCCGCCAAGCGATTGGGCA
TGAATCTTGAAGGGATTGGTGACAAGTCACTGGACATCTTGGGTAGCCATTTCCGCACGGTCACTGAATTGAGTGCGGCG
GCGGCTGAAGGGGCGGGCGCCGAACCCAAGGGCGTACGCTCCGGGGTTATGACACAGCTGGCCAAGGCGTTGGCGGAAAA
TGACTGGCGAGGCCGGCTGCAGGATGCGGAAAAAAAGGTTGCTGATATGGCTGCCCGTGCCCCCCAGGAAATGGAAAGTC
AGCCTTTAGAAGGGCAGACCTGGGTACTGACCGGGACGCTTGAGCAGTTTACCCGGAATCAGGCTAAACAGGCCCTACAG
CAGCTGGGCGCCAAAGTGGCTGGGTCGGTGTCTAAAAATACTCGCATGGTGGTGGCAGGTGCATCTGCGGGCTCCAAACT
GGCGAAAGCGGAATCGTTGGGCATTGAGGTATGCGATGAAGCTGCGCTACTGTCCCTATTGAACCAGCACGGTATTGATC
CGGGAGCGTTATGA

Upstream 100 bases:

>100_bases
TTTCCAATAACGAGGCGACAGCCCGTGTTTCAACGTTAAGCTTTACGCGTTCAACGCACCCCGCCTTTTCAATCACTAAA
CTCTCACTGCTGCGACCACT

Downstream 100 bases:

>100_bases
AAGTCTGGCTAACCCTGCTTTTGGTAGTACTATTTCTGAGTGGCTGTGCTGCCACACCGACGCATACAGATAATGTCTGT
GCGGTGTTTGATCAGCGGGG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MTNNNDPSLQAQSLRDQLNDWSYRYYVQDDPAVPDAEYDRVFRALKDLEAQYPDLVTADSPTQRVGDVPLDAFEQVQHEV
PMLSLDNAFDDEELWAFDKRIRERLDVSEIIDYVAEPKLDGLAVSLLYENGELVRAATRGDGQTGENITVNARTIRSVPL
KLRGNDVPKRLEVRGEVVMPHAGFEELNARQQEAGLKLFANPRNAAAGSLRQLDSRITASRPLEFYAYSMAQLEGWEHPP
THSAMLDALRDWGLRVNPEIRVCHGVEALLRFYQGILEKREHLDYDIDGVVYKVNRFDWQDDLGFVSRAPRWAIAHKFPA
QEELTVLNGVDWQVGRTGALTPVARLEPVHVGGVIVSNATLHNIDEIQRLDIRIGDTVVVYRAGDVIPKVVRALPERRPA
NAQGIALPSSCPVCGSEILRGHDQVVARCTGGLICGDQRREAIKHFASRRAMDIDGLGDKLVDALVDQELITTVADLYRL
KAEQVAGLERMGEKSADNLIAALEASKTVGLGRFLFALGILQIGEETAKNLADCFGDLDSIRHAPLLLLLAVPDVGLEVA
KAIGAFFAESDNEAVIDGLLAQGVSPQASGVPSAAFVKSLTLAQLLKSAKRLGMNLEGIGDKSLDILGSHFRTVTELSAA
AAEGAGAEPKGVRSGVMTQLAKALAENDWRGRLQDAEKKVADMAARAPQEMESQPLEGQTWVLTGTLEQFTRNQAKQALQ
QLGAKVAGSVSKNTRMVVAGASAGSKLAKAESLGIEVCDEAALLSLLNQHGIDPGAL

Sequences:

>Translated_777_residues
MTNNNDPSLQAQSLRDQLNDWSYRYYVQDDPAVPDAEYDRVFRALKDLEAQYPDLVTADSPTQRVGDVPLDAFEQVQHEV
PMLSLDNAFDDEELWAFDKRIRERLDVSEIIDYVAEPKLDGLAVSLLYENGELVRAATRGDGQTGENITVNARTIRSVPL
KLRGNDVPKRLEVRGEVVMPHAGFEELNARQQEAGLKLFANPRNAAAGSLRQLDSRITASRPLEFYAYSMAQLEGWEHPP
THSAMLDALRDWGLRVNPEIRVCHGVEALLRFYQGILEKREHLDYDIDGVVYKVNRFDWQDDLGFVSRAPRWAIAHKFPA
QEELTVLNGVDWQVGRTGALTPVARLEPVHVGGVIVSNATLHNIDEIQRLDIRIGDTVVVYRAGDVIPKVVRALPERRPA
NAQGIALPSSCPVCGSEILRGHDQVVARCTGGLICGDQRREAIKHFASRRAMDIDGLGDKLVDALVDQELITTVADLYRL
KAEQVAGLERMGEKSADNLIAALEASKTVGLGRFLFALGILQIGEETAKNLADCFGDLDSIRHAPLLLLLAVPDVGLEVA
KAIGAFFAESDNEAVIDGLLAQGVSPQASGVPSAAFVKSLTLAQLLKSAKRLGMNLEGIGDKSLDILGSHFRTVTELSAA
AAEGAGAEPKGVRSGVMTQLAKALAENDWRGRLQDAEKKVADMAARAPQEMESQPLEGQTWVLTGTLEQFTRNQAKQALQ
QLGAKVAGSVSKNTRMVVAGASAGSKLAKAESLGIEVCDEAALLSLLNQHGIDPGAL
>Mature_776_residues
TNNNDPSLQAQSLRDQLNDWSYRYYVQDDPAVPDAEYDRVFRALKDLEAQYPDLVTADSPTQRVGDVPLDAFEQVQHEVP
MLSLDNAFDDEELWAFDKRIRERLDVSEIIDYVAEPKLDGLAVSLLYENGELVRAATRGDGQTGENITVNARTIRSVPLK
LRGNDVPKRLEVRGEVVMPHAGFEELNARQQEAGLKLFANPRNAAAGSLRQLDSRITASRPLEFYAYSMAQLEGWEHPPT
HSAMLDALRDWGLRVNPEIRVCHGVEALLRFYQGILEKREHLDYDIDGVVYKVNRFDWQDDLGFVSRAPRWAIAHKFPAQ
EELTVLNGVDWQVGRTGALTPVARLEPVHVGGVIVSNATLHNIDEIQRLDIRIGDTVVVYRAGDVIPKVVRALPERRPAN
AQGIALPSSCPVCGSEILRGHDQVVARCTGGLICGDQRREAIKHFASRRAMDIDGLGDKLVDALVDQELITTVADLYRLK
AEQVAGLERMGEKSADNLIAALEASKTVGLGRFLFALGILQIGEETAKNLADCFGDLDSIRHAPLLLLLAVPDVGLEVAK
AIGAFFAESDNEAVIDGLLAQGVSPQASGVPSAAFVKSLTLAQLLKSAKRLGMNLEGIGDKSLDILGSHFRTVTELSAAA
AEGAGAEPKGVRSGVMTQLAKALAENDWRGRLQDAEKKVADMAARAPQEMESQPLEGQTWVLTGTLEQFTRNQAKQALQQ
LGAKVAGSVSKNTRMVVAGASAGSKLAKAESLGIEVCDEAALLSLLNQHGIDPGAL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=575, Percent_Identity=57.7391304347826, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=513, Percent_Identity=22.0272904483431, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ALCBS (Q0VR01)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692669.1
- ProteinModelPortal:   Q0VR01
- STRING:   Q0VR01
- GeneID:   4211547
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0949
- NMPDR:   fig|393595.12.peg.949
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q0VR01
- ProtClustDB:   PRK07956
- BioCyc:   ABOR393595:ABO_0949-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 84629; Mature: 84498

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 176-176 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNNDPSLQAQSLRDQLNDWSYRYYVQDDPAVPDAEYDRVFRALKDLEAQYPDLVTADS
CCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGDVPLDAFEQVQHEVPMLSLDNAFDDEELWAFDKRIRERLDVSEIIDYVAEPKLD
CHHHHCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GLAVSLLYENGELVRAATRGDGQTGENITVNARTIRSVPLKLRGNDVPKRLEVRGEVVMP
CEEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEHEECCEEECCCCCCCHHCCCCCEEEC
HAGFEELNARQQEAGLKLFANPRNAAAGSLRQLDSRITASRPLEFYAYSMAQLEGWEHPP
CCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
THSAMLDALRDWGLRVNPEIRVCHGVEALLRFYQGILEKREHLDYDIDGVVYKVNRFDWQ
CHHHHHHHHHHCCCEECCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
DDLGFVSRAPRWAIAHKFPAQEELTVLNGVDWQVGRTGALTPVARLEPVHVGGVIVSNAT
CCCHHHHCCCCEEHHCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHCCCEEECCEEEECCC
LHNIDEIQRLDIRIGDTVVVYRAGDVIPKVVRALPERRPANAQGIALPSSCPVCGSEILR
HHCHHHHHHHEEEECCEEEEEECCCHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHC
GHDQVVARCTGGLICGDQRREAIKHFASRRAMDIDGLGDKLVDALVDQELITTVADLYRL
CHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAEQVAGLERMGEKSADNLIAALEASKTVGLGRFLFALGILQIGEETAKNLADCFGDLDS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRHAPLLLLLAVPDVGLEVAKAIGAFFAESDNEAVIDGLLAQGVSPQASGVPSAAFVKSL
HHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TLAQLLKSAKRLGMNLEGIGDKSLDILGSHFRTVTELSAAAAEGAGAEPKGVRSGVMTQL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AKALAENDWRGRLQDAEKKVADMAARAPQEMESQPLEGQTWVLTGTLEQFTRNQAKQALQ
HHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
QLGAKVAGSVSKNTRMVVAGASAGSKLAKAESLGIEVCDEAALLSLLNQHGIDPGAL
HHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNNNDPSLQAQSLRDQLNDWSYRYYVQDDPAVPDAEYDRVFRALKDLEAQYPDLVTADS
CCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGDVPLDAFEQVQHEVPMLSLDNAFDDEELWAFDKRIRERLDVSEIIDYVAEPKLD
CHHHHCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GLAVSLLYENGELVRAATRGDGQTGENITVNARTIRSVPLKLRGNDVPKRLEVRGEVVMP
CEEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEHEECCEEECCCCCCCHHCCCCCEEEC
HAGFEELNARQQEAGLKLFANPRNAAAGSLRQLDSRITASRPLEFYAYSMAQLEGWEHPP
CCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
THSAMLDALRDWGLRVNPEIRVCHGVEALLRFYQGILEKREHLDYDIDGVVYKVNRFDWQ
CHHHHHHHHHHCCCEECCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
DDLGFVSRAPRWAIAHKFPAQEELTVLNGVDWQVGRTGALTPVARLEPVHVGGVIVSNAT
CCCHHHHCCCCEEHHCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHCCCEEECCEEEECCC
LHNIDEIQRLDIRIGDTVVVYRAGDVIPKVVRALPERRPANAQGIALPSSCPVCGSEILR
HHCHHHHHHHEEEECCEEEEEECCCHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHC
GHDQVVARCTGGLICGDQRREAIKHFASRRAMDIDGLGDKLVDALVDQELITTVADLYRL
CHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAEQVAGLERMGEKSADNLIAALEASKTVGLGRFLFALGILQIGEETAKNLADCFGDLDS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRHAPLLLLLAVPDVGLEVAKAIGAFFAESDNEAVIDGLLAQGVSPQASGVPSAAFVKSL
HHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TLAQLLKSAKRLGMNLEGIGDKSLDILGSHFRTVTELSAAAAEGAGAEPKGVRSGVMTQL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AKALAENDWRGRLQDAEKKVADMAARAPQEMESQPLEGQTWVLTGTLEQFTRNQAKQALQ
HHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
QLGAKVAGSVSKNTRMVVAGASAGSKLAKAESLGIEVCDEAALLSLLNQHGIDPGAL
HHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA