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The map label for this gene is pleD [H]

Identifier: 110833577

GI number: 110833577

Start: 804542

End: 805645

Strand: Direct

Name: pleD [H]

Synonym: ABO_0716

Alternate gene names: 110833577

Gene position: 804542-805645 (Clockwise)

Preceding gene: 110833576

Following gene: 110833581

Centisome position: 25.79

GC content: 53.99

Gene sequence:

>1104_bases
ATGAAGCATGATTTGCATCAGGCGGAAAAGCTGAGACTGAGCGAGAAACGCTTGATGCAAAGGCATAGTCGTTCTGCCAT
GGTAATCATCGCTATGCACACCCTATTGTGCGTGGTTTATTTTCAGCTTGGCTACATGGATATCTCGCTGACGGAGGCGT
TGGTGCTGGCTTTGCTGGTCTGGGGCTCACTGTTGGCTTATCAGTTGCTGATGGCAACAGGCGTCACCTTTAGGCTGCGT
GAGCCGAGCTTATCGTTACCGTTAATCCTGTATTTTATCGGCGTGTTCATGGTGTCGGGCTATTACGTGGATGAGTTTCG
GCTCAGCGTGGTAACGCTATTTTTTTCCGCGTTATTGTTAGTGTCGTTTCGGCTCAGCGGTAAAGTCATGGTGGCAGTGG
CGTTTATGGCCAGTGCGGCCTATGCGTTGATGCTGTGGCTGGCTCTGCTGGATCGTTGGGTACACTTGAGTCTGTCAGTG
GAGCTGTTGCAATGGTTGGTATTTGCCATGATTTCGGTGAGCTTTGCCGTCACCGGTGGCAGTATCAATCGCCTTCGCGA
CGCTCTGGTCGATAAAAATCGTGACCTCGCCAATGCAGTGAAAAAGGTACGGGAAATGGCCATTCGTGATGACCTCACTG
GCCTGTTTAACCGCCGCCACTTACTGGAAATTCTGGAGCGCCAGAGGGCTTTGTTCAGCAGTAAGAGCGTGCCGTTTGCG
GTGTGTTACGTGGATCTTGACCACTTTAAGAAAATTAATGATCGTCATGGCCATGACTGGGGCGATCGAGTGCTGCGTCA
GTTTTCCGAGTTGGCGTTGTCGGGAATGCGTGATGGCGATCACTTTGGCCGCTTGGGTGGCGAAGAGTTTCTGCTGGTGT
TGCCGCAGAGTGACGCCCAAGGTGCTTTACTGGTGGCAGAACGCTTGCGCTTGCGTTGGCGCGAACAACGCTTCGATGCT
CAAGGTGGGCCGGCGATAGTCAGCCTGTCTGTGGGCGTGGCGGCTTACCAGGAGGGAGAGCGCGTGGACGAGTTGCTTAA
CCGGGCGGATCAGGGCTTATATAGGGCAAAGGCCGGGGGGCGGGACCAGAGCCGAGTTGCATGA

Upstream 100 bases:

>100_bases
CGGATGGTGAAACTCCTGCACAATTGATTAAACGAGCCGATCTCGCCTTGTATCGGGCCAAGGAAACCGGACGCGATAAA
CTGATTATCGGGGAGACCGA

Downstream 100 bases:

>100_bases
GTTGAAAGTCGAACAATGCGATCAGCGGGCTCCGCTAGACGATAAACTCCTGTGCCGCAGAGCAAGTCATTTACGATGGA
AAGGGTCTCATTGTTGTAGC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKHDLHQAEKLRLSEKRLMQRHSRSAMVIIAMHTLLCVVYFQLGYMDISLTEALVLALLVWGSLLAYQLLMATGVTFRLR
EPSLSLPLILYFIGVFMVSGYYVDEFRLSVVTLFFSALLLVSFRLSGKVMVAVAFMASAAYALMLWLALLDRWVHLSLSV
ELLQWLVFAMISVSFAVTGGSINRLRDALVDKNRDLANAVKKVREMAIRDDLTGLFNRRHLLEILERQRALFSSKSVPFA
VCYVDLDHFKKINDRHGHDWGDRVLRQFSELALSGMRDGDHFGRLGGEEFLLVLPQSDAQGALLVAERLRLRWREQRFDA
QGGPAIVSLSVGVAAYQEGERVDELLNRADQGLYRAKAGGRDQSRVA

Sequences:

>Translated_367_residues
MKHDLHQAEKLRLSEKRLMQRHSRSAMVIIAMHTLLCVVYFQLGYMDISLTEALVLALLVWGSLLAYQLLMATGVTFRLR
EPSLSLPLILYFIGVFMVSGYYVDEFRLSVVTLFFSALLLVSFRLSGKVMVAVAFMASAAYALMLWLALLDRWVHLSLSV
ELLQWLVFAMISVSFAVTGGSINRLRDALVDKNRDLANAVKKVREMAIRDDLTGLFNRRHLLEILERQRALFSSKSVPFA
VCYVDLDHFKKINDRHGHDWGDRVLRQFSELALSGMRDGDHFGRLGGEEFLLVLPQSDAQGALLVAERLRLRWREQRFDA
QGGPAIVSLSVGVAAYQEGERVDELLNRADQGLYRAKAGGRDQSRVA
>Mature_367_residues
MKHDLHQAEKLRLSEKRLMQRHSRSAMVIIAMHTLLCVVYFQLGYMDISLTEALVLALLVWGSLLAYQLLMATGVTFRLR
EPSLSLPLILYFIGVFMVSGYYVDEFRLSVVTLFFSALLLVSFRLSGKVMVAVAFMASAAYALMLWLALLDRWVHLSLSV
ELLQWLVFAMISVSFAVTGGSINRLRDALVDKNRDLANAVKKVREMAIRDDLTGLFNRRHLLEILERQRALFSSKSVPFA
VCYVDLDHFKKINDRHGHDWGDRVLRQFSELALSGMRDGDHFGRLGGEEFLLVLPQSDAQGALLVAERLRLRWREQRFDA
QGGPAIVSLSVGVAAYQEGERVDELLNRADQGLYRAKAGGRDQSRVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=34.9112426035503, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI145693134, Length=155, Percent_Identity=38.0645161290323, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=42.5, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=36.8098159509202, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=31.6091954022989, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787802, Length=264, Percent_Identity=26.1363636363636, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=32.2033898305085, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=35.0877192982456, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=30.7228915662651, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=30.3225806451613, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=183, Percent_Identity=31.6939890710383, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41534; Mature: 41534

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCC
>Mature Secondary Structure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CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA