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The map label for this gene is ampD [H]

Identifier: 110833481

GI number: 110833481

Start: 682942

End: 683508

Strand: Direct

Name: ampD [H]

Synonym: ABO_0620

Alternate gene names: 110833481

Gene position: 682942-683508 (Clockwise)

Preceding gene: 110833480

Following gene: 110833482

Centisome position: 21.89

GC content: 52.2

Gene sequence:

>567_bases
GTGCTTACCCTTTCCGAACATCGAGTCGACCAGGCGCAATGGTGCCCCTCCCCTAATTTCAACGATCGTCCTGATCCTGC
TGATATCTCCCTGCTGGTGATTCACAACATCAGCTTGCCCCCGGGACAATTTGGTAGTGGCCATATCCAGGCGTTGTTTC
AGAACTGTCTTGATCCGGCGCAACACCCTTATTTTGAAACCATCCACCAGCTGACGGTGTCGGCTCACTTCTTGATTCGT
CGCAATGGCAGCCTGTTGCAGTTTGTGTCGTGCGACAAGCGTGCCTGGCATGCTGGGCTCTCGGCGTTCCAGGGCCGTGA
AAACTGTAATGATTTTTCTATCGGTATCGAGCTAGAAGGTGCCGATACAATGCCTTATGAAACCGCCCAGTACGTTACCC
TTGTTGCGCTTACGGGACTTTTGCAGCATCACTATCCTGCTATTGTCGACGCTCGTATTGCTGGCCATGAGCACATTGCG
CCGGGAAGAAAAACGGATCCGGGCCCTGCCTTCGACTGGTTGCGTTATCGTCAGGAATTAGCCAATTTTAAGGAGAAAAG
AGCATGA

Upstream 100 bases:

>100_bases
AGTTATTGGCGACCTGCGTCGTATGCAAGGCAATGTGCAGCACTAAGGGCTAAAGTGCAGCACCGGCTTCCTTAGGCGCA
GGCAAGAGGAGTTTGCGCGG

Downstream 100 bases:

>100_bases
AGTTTATCGTTCTTATTGTGGTTATGGGGTTGCGACGGATGGACCTTGGTTGGTCTACATTGCTGGCAGATGATGAACGG
CATCAGCGTTGGTTGCAGCG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MLTLSEHRVDQAQWCPSPNFNDRPDPADISLLVIHNISLPPGQFGSGHIQALFQNCLDPAQHPYFETIHQLTVSAHFLIR
RNGSLLQFVSCDKRAWHAGLSAFQGRENCNDFSIGIELEGADTMPYETAQYVTLVALTGLLQHHYPAIVDARIAGHEHIA
PGRKTDPGPAFDWLRYRQELANFKEKRA

Sequences:

>Translated_188_residues
MLTLSEHRVDQAQWCPSPNFNDRPDPADISLLVIHNISLPPGQFGSGHIQALFQNCLDPAQHPYFETIHQLTVSAHFLIR
RNGSLLQFVSCDKRAWHAGLSAFQGRENCNDFSIGIELEGADTMPYETAQYVTLVALTGLLQHHYPAIVDARIAGHEHIA
PGRKTDPGPAFDWLRYRQELANFKEKRA
>Mature_188_residues
MLTLSEHRVDQAQWCPSPNFNDRPDPADISLLVIHNISLPPGQFGSGHIQALFQNCLDPAQHPYFETIHQLTVSAHFLIR
RNGSLLQFVSCDKRAWHAGLSAFQGRENCNDFSIGIELEGADTMPYETAQYVTLVALTGLLQHHYPAIVDARIAGHEHIA
PGRKTDPGPAFDWLRYRQELANFKEKRA

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=166, Percent_Identity=59.0361445783133, Blast_Score=197, Evalue=2e-52,
Organism=Escherichia coli, GI1787092, Length=129, Percent_Identity=37.2093023255814, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21152; Mature: 21152

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLSEHRVDQAQWCPSPNFNDRPDPADISLLVIHNISLPPGQFGSGHIQALFQNCLDPA
CCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCC
QHPYFETIHQLTVSAHFLIRRNGSLLQFVSCDKRAWHAGLSAFQGRENCNDFSIGIELEG
CCHHHHHHHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECC
ADTMPYETAQYVTLVALTGLLQHHYPAIVDARIAGHEHIAPGRKTDPGPAFDWLRYRQEL
CCCCCCHHHHHHHHHHHHHHHHHCCCHHEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
ANFKEKRA
HHHHHHCC
>Mature Secondary Structure
MLTLSEHRVDQAQWCPSPNFNDRPDPADISLLVIHNISLPPGQFGSGHIQALFQNCLDPA
CCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCC
QHPYFETIHQLTVSAHFLIRRNGSLLQFVSCDKRAWHAGLSAFQGRENCNDFSIGIELEG
CCHHHHHHHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECC
ADTMPYETAQYVTLVALTGLLQHHYPAIVDARIAGHEHIAPGRKTDPGPAFDWLRYRQEL
CCCCCCHHHHHHHHHHHHHHHHHCCCHHEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
ANFKEKRA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]