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The map label for this gene is fixJ [H]

Identifier: 110680275

GI number: 110680275

Start: 2960113

End: 2960754

Strand: Direct

Name: fixJ [H]

Synonym: RD1_3086

Alternate gene names: 110680275

Gene position: 2960113-2960754 (Clockwise)

Preceding gene: 110680274

Following gene: 110680278

Centisome position: 71.62

GC content: 56.85

Gene sequence:

>642_bases
ATGAATACAGCCAGTGAACCTTGCGTTTTCATCGTGGACGACGACGAAGACATTCGCACCTCGCTGTCGCGCGCCTTGCG
GACACGCGGGTACACAACCGAGGTCTTTGACTCTGCACAGGCTTTTCTGGCGGGCTATGATGCTGCGCGATCGGGGTGCC
TTATCCTTGATTACGGGCTACCGCAGATGAACGGTCTGGACCTGCAGCAGGTGCTTGTGAAGCGGGGCCGACCCTTGCCC
ATCATTTTCATCACCGGGCACGGGGGCGTATCCGAAGCCGTGCGGGCAATGAAACATGGCGCGGTGGATTTTCTGGAAAA
ACCGTTTCGTCAGGAGACATTGCTGGCCCGAATTGAAACGGCATTGGAAATGGATGCAAAGGCACGCGATGTCGATACCG
CGCTAAAGCTGACCCGCGACAAGCTGGCAAGCCTGACGCAGCGCGAAAGGGAAATCGCAGAGTTTCTGGTGATGAATCCG
TCCAACAGTTCCAGCAAGGATGTGGCGCGTCATCTGGACATCAGCCCCCGCACGGTCGATCACCACCGCGCGCGCATTCT
GGAAAAGATGAATGTCCAATCCGTCGCCGAGCTTGTTGATCTCGCCCTGACTTATCAGCTTTTTACAAAGCCCGACCCCT
GA

Upstream 100 bases:

>100_bases
CATCGCGGGGAGATCTGGCATCAGCAGGACGCCGATGGAAACCCGGAATTCTGCTTTACGCTGCCTGCCTTGAAACCAAA
GACCGAAGCGGGGAAGGCGT

Downstream 100 bases:

>100_bases
AGCGTCATGCGACAAACCTGACTTACGGAACGCTGCCTGACATCAAAGATTTCAACGCGTTACGTGATACCCGATGTGTC
AGGCATTGCGTCAGACGGCC

Product: transcriptional regulatory protein FixJ, putative

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNTASEPCVFIVDDDEDIRTSLSRALRTRGYTTEVFDSAQAFLAGYDAARSGCLILDYGLPQMNGLDLQQVLVKRGRPLP
IIFITGHGGVSEAVRAMKHGAVDFLEKPFRQETLLARIETALEMDAKARDVDTALKLTRDKLASLTQREREIAEFLVMNP
SNSSSKDVARHLDISPRTVDHHRARILEKMNVQSVAELVDLALTYQLFTKPDP

Sequences:

>Translated_213_residues
MNTASEPCVFIVDDDEDIRTSLSRALRTRGYTTEVFDSAQAFLAGYDAARSGCLILDYGLPQMNGLDLQQVLVKRGRPLP
IIFITGHGGVSEAVRAMKHGAVDFLEKPFRQETLLARIETALEMDAKARDVDTALKLTRDKLASLTQREREIAEFLVMNP
SNSSSKDVARHLDISPRTVDHHRARILEKMNVQSVAELVDLALTYQLFTKPDP
>Mature_213_residues
MNTASEPCVFIVDDDEDIRTSLSRALRTRGYTTEVFDSAQAFLAGYDAARSGCLILDYGLPQMNGLDLQQVLVKRGRPLP
IIFITGHGGVSEAVRAMKHGAVDFLEKPFRQETLLARIETALEMDAKARDVDTALKLTRDKLASLTQREREIAEFLVMNP
SNSSSKDVARHLDISPRTVDHHRARILEKMNVQSVAELVDLALTYQLFTKPDP

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=35.6521739130435, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=164, Percent_Identity=28.6585365853659, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788550, Length=123, Percent_Identity=25.2032520325203, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790860, Length=114, Percent_Identity=29.8245614035088, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788222, Length=214, Percent_Identity=24.2990654205607, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=30.2521008403361, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23770; Mature: 23770

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTASEPCVFIVDDDEDIRTSLSRALRTRGYTTEVFDSAQAFLAGYDAARSGCLILDYGL
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
PQMNGLDLQQVLVKRGRPLPIIFITGHGGVSEAVRAMKHGAVDFLEKPFRQETLLARIET
CCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
ALEMDAKARDVDTALKLTRDKLASLTQREREIAEFLVMNPSNSSSKDVARHLDISPRTVD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCCHH
HHRARILEKMNVQSVAELVDLALTYQLFTKPDP
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTASEPCVFIVDDDEDIRTSLSRALRTRGYTTEVFDSAQAFLAGYDAARSGCLILDYGL
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
PQMNGLDLQQVLVKRGRPLPIIFITGHGGVSEAVRAMKHGAVDFLEKPFRQETLLARIET
CCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
ALEMDAKARDVDTALKLTRDKLASLTQREREIAEFLVMNPSNSSSKDVARHLDISPRTVD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCCHH
HHRARILEKMNVQSVAELVDLALTYQLFTKPDP
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]