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The map label for this gene is fixL [H]

Identifier: 110680274

GI number: 110680274

Start: 2957784

End: 2960129

Strand: Direct

Name: fixL [H]

Synonym: RD1_3085

Alternate gene names: 110680274

Gene position: 2957784-2960129 (Clockwise)

Preceding gene: 110680273

Following gene: 110680275

Centisome position: 71.56

GC content: 57.2

Gene sequence:

>2346_bases
ATGAAGTTCTCTCTGGGTTTGATACTGGCCCTTTGCCTCGCGGGATTGCAATTTGTTGCCGTCACCATTGTGGTGTTTTC
ATCCTTCCTGACATCGGAAAAGGTGCTGCTTGAGCACGCGCGCGATCTGCTCAGCGATGTGGGAACCAATACGATCGAGC
ACTCCAAAGGGTTCCTGAAGCCCGCCAAGGGGGCTGCCGAACTGGCCACGCGGCTTGCCGAGAACGAGGTGATCGCCAGC
GATGACTTCAACCAGTTGGAAAATCTGCTGTTTCAGCAGCTTCAGATATCGCCACAGTTCGCCGGGGTCTTTTACGGCGA
TGAGGCGGGAAATTTCGTCTATGTGATGCGCAGCGAAGGGCCCGGACCTTTCCGCACCAAGATCGTTCGGCGCGATGGCG
AGGCGCGCGAAACCGAGCTGATCTGGCGTGACACAAATTATCAGGTGCTGGACCGGGAGGAAGACGAGGCGGATACATAT
GATCCGCGCACGCGCCCGTGGTATCAGTCATCACGGGAAAATCTGACCGGGATCTGGACCGACCCCTATATTTTCTTCAC
CTCTCAACGTCCGGGCATCACCGCCGCCTCACCCGTTTTCAACACCGATGGCGAATTGCGCGGCGTGGTGGGCGTGGACA
TTGAAATTGGCGCGATTTCCGAATTCCTCAGTCGGCTCAATATCGGAGACTCGGGCGTTGCGCTGATCCTGAACCAGAAC
GGCGATGTCATTGCACACCCTGATCAGGACCTGATCCGCAAGGAAAATGCCGATGGGACGTTTCGCTTCGTGAACATCAA
CGAGATCGACGATCCGGTGGCCCATGCGGCTTTTGCCGAGGTGATCCGTGGTTCTGAGGTCAATGTGGATCGTGAGCTTG
CATCCCGGTTTGAGTTTGACGGCTCGACCTATGTCTCAACCGTGATGCCCGCCATCAGCGACGAGTTGCCCTGGACGATT
GCGGTCTATGCGCCCGAAGCGGATTTCACCGGAGAGATCACCGGCAACCGCACACAGAACGTCTGGATCGCGGCGGCAAT
TGCGCTGGCATCGGGAGTGGTCGGGTTGATGCTCGCCAGCTATATCCACAAACCTGTGCGCGCCTTTGCGGTGCGCTCCT
CGCTGATCTCGCAAGGGGAGGCCTCTGCGGAGGAGCCATTGCCCAAGACCTACAAGGAGCTTGAACGGGCAAATGAAACG
CTGGTTCAGGCCATTGCAGAGCGCAAGAAGTCAGAGGCGGAATACGACCGCACCTTTGATCTCGCCGCGCGGGGCATGGC
GCGGATACAGGCGCATACGGGCCGGATTCTGCGTATCAACCGACGGCTTGCCGATATGCTGGGCTATGGCGAAGCCGAAG
CGCTCGAGTTGACCATTCAGGACATCTCACACCCCAATGACCCGGTGGCCAAGGTGTTTACCGCATCAAGTGGCGGGGGC
CGGTCCGAATATTTCAACGAAAAACGGTATCGGCACAAAGATGGCTCCACCATTTGGGTCAGCGAAAACGTCATTGTCAT
CCGCGACGACAATGACGTGCCCGTCTATGCCGTGGTTGCGGTCGATGACATCTCAGAAAGGATCGCCGCAGATCTGAAGA
TCCAGCAGTTGAACACGGATCTGTCCCATTCTGCGCGCATCAACATGATGGGGCAGATGGCAACCGGGCTTGCCCATGAA
TTGAACCAGCCGTTGCTTGCGATATCCCAGAATATGGACACGGCTGTTTTCCTGCTCAAAGACGCCGGTCAGGAGGCGGG
TGAGTTGCAGGAGTTGCTTTTGGAAACCGACCGACACGCCCATCGCGCCGGGGATATCATCAAGGCACTGCGGGCTTTCG
TCAAAAAGGAGGGCGCCGAAAAGGCGGAGTTTGATTTCGCCGAACTGATGGAGCAATCCTTGCACCTGATGCGTGCCGAG
GCAAAGGACCACGGCATCAAGATCACCGCTGTCGGCGCGGACCTCGAACCTGTGTACGGATCCCGCGTGCAGATCGCGCA
GGTGATCGTGAACATGTTGCGCAATGCGATCGAATCCATTTCCCAGAATGACGCGGCCGAGCGCACGGTGACCCTGAAGG
CAGAGAATACTCCTTCCGGGATCCTCGTCCGCGTCACAGACACAGGACCCGGTTTGCAGGAAAATGTGGACATTTTCGAA
CACTTCAAAACAACCAAGAAAGATGGCATGGGCATGGGTCTTTCGATCTGCCGCACCATAGTGGAATCGCATCGCGGGGA
GATCTGGCATCAGCAGGACGCCGATGGAAACCCGGAATTCTGCTTTACGCTGCCTGCCTTGAAACCAAAGACCGAAGCGG
GGAAGGCGTATGAATACAGCCAGTGA

Upstream 100 bases:

>100_bases
ACATACCCTCGCGCCCGCCGTCATGGCATGGGCGATAAAAGGCTCAGCAGGTTTTGTTGGGTCTTAACAATCCCTTGGTT
TTCGGTGAAACTGCGGAGTG

Downstream 100 bases:

>100_bases
ACCTTGCGTTTTCATCGTGGACGACGACGAAGACATTCGCACCTCGCTGTCGCGCGCCTTGCGGACACGCGGGTACACAA
CCGAGGTCTTTGACTCTGCA

Product: sensor protein FixL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 781; Mature: 781

Protein sequence:

>781_residues
MKFSLGLILALCLAGLQFVAVTIVVFSSFLTSEKVLLEHARDLLSDVGTNTIEHSKGFLKPAKGAAELATRLAENEVIAS
DDFNQLENLLFQQLQISPQFAGVFYGDEAGNFVYVMRSEGPGPFRTKIVRRDGEARETELIWRDTNYQVLDREEDEADTY
DPRTRPWYQSSRENLTGIWTDPYIFFTSQRPGITAASPVFNTDGELRGVVGVDIEIGAISEFLSRLNIGDSGVALILNQN
GDVIAHPDQDLIRKENADGTFRFVNINEIDDPVAHAAFAEVIRGSEVNVDRELASRFEFDGSTYVSTVMPAISDELPWTI
AVYAPEADFTGEITGNRTQNVWIAAAIALASGVVGLMLASYIHKPVRAFAVRSSLISQGEASAEEPLPKTYKELERANET
LVQAIAERKKSEAEYDRTFDLAARGMARIQAHTGRILRINRRLADMLGYGEAEALELTIQDISHPNDPVAKVFTASSGGG
RSEYFNEKRYRHKDGSTIWVSENVIVIRDDNDVPVYAVVAVDDISERIAADLKIQQLNTDLSHSARINMMGQMATGLAHE
LNQPLLAISQNMDTAVFLLKDAGQEAGELQELLLETDRHAHRAGDIIKALRAFVKKEGAEKAEFDFAELMEQSLHLMRAE
AKDHGIKITAVGADLEPVYGSRVQIAQVIVNMLRNAIESISQNDAAERTVTLKAENTPSGILVRVTDTGPGLQENVDIFE
HFKTTKKDGMGMGLSICRTIVESHRGEIWHQQDADGNPEFCFTLPALKPKTEAGKAYEYSQ

Sequences:

>Translated_781_residues
MKFSLGLILALCLAGLQFVAVTIVVFSSFLTSEKVLLEHARDLLSDVGTNTIEHSKGFLKPAKGAAELATRLAENEVIAS
DDFNQLENLLFQQLQISPQFAGVFYGDEAGNFVYVMRSEGPGPFRTKIVRRDGEARETELIWRDTNYQVLDREEDEADTY
DPRTRPWYQSSRENLTGIWTDPYIFFTSQRPGITAASPVFNTDGELRGVVGVDIEIGAISEFLSRLNIGDSGVALILNQN
GDVIAHPDQDLIRKENADGTFRFVNINEIDDPVAHAAFAEVIRGSEVNVDRELASRFEFDGSTYVSTVMPAISDELPWTI
AVYAPEADFTGEITGNRTQNVWIAAAIALASGVVGLMLASYIHKPVRAFAVRSSLISQGEASAEEPLPKTYKELERANET
LVQAIAERKKSEAEYDRTFDLAARGMARIQAHTGRILRINRRLADMLGYGEAEALELTIQDISHPNDPVAKVFTASSGGG
RSEYFNEKRYRHKDGSTIWVSENVIVIRDDNDVPVYAVVAVDDISERIAADLKIQQLNTDLSHSARINMMGQMATGLAHE
LNQPLLAISQNMDTAVFLLKDAGQEAGELQELLLETDRHAHRAGDIIKALRAFVKKEGAEKAEFDFAELMEQSLHLMRAE
AKDHGIKITAVGADLEPVYGSRVQIAQVIVNMLRNAIESISQNDAAERTVTLKAENTPSGILVRVTDTGPGLQENVDIFE
HFKTTKKDGMGMGLSICRTIVESHRGEIWHQQDADGNPEFCFTLPALKPKTEAGKAYEYSQ
>Mature_781_residues
MKFSLGLILALCLAGLQFVAVTIVVFSSFLTSEKVLLEHARDLLSDVGTNTIEHSKGFLKPAKGAAELATRLAENEVIAS
DDFNQLENLLFQQLQISPQFAGVFYGDEAGNFVYVMRSEGPGPFRTKIVRRDGEARETELIWRDTNYQVLDREEDEADTY
DPRTRPWYQSSRENLTGIWTDPYIFFTSQRPGITAASPVFNTDGELRGVVGVDIEIGAISEFLSRLNIGDSGVALILNQN
GDVIAHPDQDLIRKENADGTFRFVNINEIDDPVAHAAFAEVIRGSEVNVDRELASRFEFDGSTYVSTVMPAISDELPWTI
AVYAPEADFTGEITGNRTQNVWIAAAIALASGVVGLMLASYIHKPVRAFAVRSSLISQGEASAEEPLPKTYKELERANET
LVQAIAERKKSEAEYDRTFDLAARGMARIQAHTGRILRINRRLADMLGYGEAEALELTIQDISHPNDPVAKVFTASSGGG
RSEYFNEKRYRHKDGSTIWVSENVIVIRDDNDVPVYAVVAVDDISERIAADLKIQQLNTDLSHSARINMMGQMATGLAHE
LNQPLLAISQNMDTAVFLLKDAGQEAGELQELLLETDRHAHRAGDIIKALRAFVKKEGAEKAEFDFAELMEQSLHLMRAE
AKDHGIKITAVGADLEPVYGSRVQIAQVIVNMLRNAIESISQNDAAERTVTLKAENTPSGILVRVTDTGPGLQENVDIFE
HFKTTKKDGMGMGLSICRTIVESHRGEIWHQQDADGNPEFCFTLPALKPKTEAGKAYEYSQ

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=27.3504273504274, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=24.7787610619469, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788381, Length=141, Percent_Identity=30.4964539007092, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 86298; Mature: 86298

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSLGLILALCLAGLQFVAVTIVVFSSFLTSEKVLLEHARDLLSDVGTNTIEHSKGFLK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
PAKGAAELATRLAENEVIASDDFNQLENLLFQQLQISPQFAGVFYGDEAGNFVYVMRSEG
CCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
PGPFRTKIVRRDGEARETELIWRDTNYQVLDREEDEADTYDPRTRPWYQSSRENLTGIWT
CCHHHHHHHHCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCHHCCCCCCCEEEC
DPYIFFTSQRPGITAASPVFNTDGELRGVVGVDIEIGAISEFLSRLNIGDSGVALILNQN
CCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHCCCCCCCEEEEECCC
GDVIAHPDQDLIRKENADGTFRFVNINEIDDPVAHAAFAEVIRGSEVNVDRELASRFEFD
CCEEECCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
GSTYVSTVMPAISDELPWTIAVYAPEADFTGEITGNRTQNVWIAAAIALASGVVGLMLAS
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YIHKPVRAFAVRSSLISQGEASAEEPLPKTYKELERANETLVQAIAERKKSEAEYDRTFD
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARGMARIQAHTGRILRINRRLADMLGYGEAEALELTIQDISHPNDPVAKVFTASSGGG
HHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCEEEEEEHHCCCCCCCHHEEEECCCCCC
RSEYFNEKRYRHKDGSTIWVSENVIVIRDDNDVPVYAVVAVDDISERIAADLKIQQLNTD
CHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCEEEEEEEHHHHHHHHHCCCCEECCCC
LSHSARINMMGQMATGLAHELNQPLLAISQNMDTAVFLLKDAGQEAGELQELLLETDRHA
CCCCHHHHHHHHHHHHHHHHHCCCCEEHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
HRAGDIIKALRAFVKKEGAEKAEFDFAELMEQSLHLMRAEAKDHGIKITAVGADLEPVYG
HHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
SRVQIAQVIVNMLRNAIESISQNDAAERTVTLKAENTPSGILVRVTDTGPGLQENVDIFE
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHCHHHHH
HFKTTKKDGMGMGLSICRTIVESHRGEIWHQQDADGNPEFCFTLPALKPKTEAGKAYEYS
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
Q
C
>Mature Secondary Structure
MKFSLGLILALCLAGLQFVAVTIVVFSSFLTSEKVLLEHARDLLSDVGTNTIEHSKGFLK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
PAKGAAELATRLAENEVIASDDFNQLENLLFQQLQISPQFAGVFYGDEAGNFVYVMRSEG
CCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
PGPFRTKIVRRDGEARETELIWRDTNYQVLDREEDEADTYDPRTRPWYQSSRENLTGIWT
CCHHHHHHHHCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCHHCCCCCCCEEEC
DPYIFFTSQRPGITAASPVFNTDGELRGVVGVDIEIGAISEFLSRLNIGDSGVALILNQN
CCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHCCCCCCCEEEEECCC
GDVIAHPDQDLIRKENADGTFRFVNINEIDDPVAHAAFAEVIRGSEVNVDRELASRFEFD
CCEEECCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
GSTYVSTVMPAISDELPWTIAVYAPEADFTGEITGNRTQNVWIAAAIALASGVVGLMLAS
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YIHKPVRAFAVRSSLISQGEASAEEPLPKTYKELERANETLVQAIAERKKSEAEYDRTFD
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARGMARIQAHTGRILRINRRLADMLGYGEAEALELTIQDISHPNDPVAKVFTASSGGG
HHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCEEEEEEHHCCCCCCCHHEEEECCCCCC
RSEYFNEKRYRHKDGSTIWVSENVIVIRDDNDVPVYAVVAVDDISERIAADLKIQQLNTD
CHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCEEEEEEEHHHHHHHHHCCCCEECCCC
LSHSARINMMGQMATGLAHELNQPLLAISQNMDTAVFLLKDAGQEAGELQELLLETDRHA
CCCCHHHHHHHHHHHHHHHHHCCCCEEHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
HRAGDIIKALRAFVKKEGAEKAEFDFAELMEQSLHLMRAEAKDHGIKITAVGADLEPVYG
HHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
SRVQIAQVIVNMLRNAIESISQNDAAERTVTLKAENTPSGILVRVTDTGPGLQENVDIFE
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHCHHHHH
HFKTTKKDGMGMGLSICRTIVESHRGEIWHQQDADGNPEFCFTLPALKPKTEAGKAYEYS
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]