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The map label for this gene is prfB

Identifier: 110680026

GI number: 110680026

Start: 2687396

End: 2688520

Strand: Reverse

Name: prfB

Synonym: RD1_2819

Alternate gene names: 110680026

Gene position: 2688520-2687396 (Counterclockwise)

Preceding gene: 110680027

Following gene: 110680025

Centisome position: 65.05

GC content: 58.49

Gene sequence:

>1125_bases
ATGCGCGCAGACGTCCAGAACACCATTGAAAAAATACAGAAATCGCTGGACCTTCTGGCCCAGCGGCTGGATGTGGAAAC
CGCCCCCTACAGGCTTGAGGAATTCAATGCGCGGGTGGAAGACCCGACCCTTTGGGATGATCCGGATGCGGCGCAGAAAC
TGATGCGGGAGCGTCAGATGCTGGTGGATGCCATGGCGACCCACGACAGCATTAAGCAGGAGATGGCCGACAACATCGAA
CTGATCGAACTTGGTGAGATGGAAGACGACGCAGATGTGGTCTCTGACGCTGAAAACGCATTGCGCGCTTTGGAAGAGAC
CGCGGCGAAGAAGGAACTCGAAGCGCTGCTGGACGGTGAAGCGGACAGCAACGACACCTTTCTGGAGGTCAACGCCGGAG
CAGGGGGCACCGAAAGCTGTGACTGGGCGTCGATGCTGGCGCGCATGTATGTCCGCTGGGCGGAGAAGAAAGGCTACAAG
GTCGAATTGCAGTCCATGAGCGACGGCGACGAGGCGGGGATCAAATCCGCGACCTACAAGATCACCGGGTTGAATGCCTA
TGGGTGGCTCAAATCGGAAAGCGGCGTGCACCGCCTCGTGCGTATCTCGCCTTTTGACAGTGCGGCCAAGCGGCATACGT
CCTTCAGCTCGGTCTGGGTGTATCCGGTGGTGGATGACAATATCGACATCGAGGTGAATCCGGCAGACATCCGCATTGAC
ACCTACCGCTCCTCAGGCGCGGGGGGGCAACACGTCAACACGACGGATTCCGCCGTTCGGATCACGCACCACCCGACGGG
CATCGTGGTGACCAGTTCGGAAAAGTCCCAGCACCAGAACCGCGACATCGCGATGAAGGCGCTGAAGTCGCGCCTGTATC
AACTGGAACTGGACCGGCGCAACGCGGCCATCAACGAAGCGCATGAAAACAAGGGTGATGCCGGATGGGGCAATCAGATC
CGGTCCTACGTTTTGCAGCCCTATCAGATGGTCAAAGACCTGCGCACCAATTTTGAAACCTCCGACACCAAGGGCGTTCT
GGATGGTGATCTGGACGGGTTCATGGCGGCAACCCTTGCATTGGATGCATCCGGCAAAACACGCGCCGAAGCGCAAAACG
GCTGA

Upstream 100 bases:

>100_bases
GGCGGTCTATACTGAACACGGGTGTGTAATCGCCCCGGGTGCCTTGCCGCTGCTGTGGTGCTGGTCTATCAGGATGTAAC
ACTTTTTTGCCGAGGCTGAC

Downstream 100 bases:

>100_bases
TCAATTCCATTTTGACATGCGACCGCAACTCGGGCACGTTTTAGTCGGTTGAGTACGAAGAGGAGACTTCCGTGGCGGCG
ATTGCAGAGGGAGAATTGAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQMLVDAMATHDSIKQEMADNIE
LIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGEADSNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYK
VELQSMSDGDEAGIKSATYKITGLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVVDDNIDIEVNPADIRID
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRNAAINEAHENKGDAGWGNQI
RSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMAATLALDASGKTRAEAQNG

Sequences:

>Translated_374_residues
MRADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQMLVDAMATHDSIKQEMADNIE
LIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGEADSNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYK
VELQSMSDGDEAGIKSATYKITGLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVVDDNIDIEVNPADIRID
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRNAAINEAHENKGDAGWGNQI
RSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMAATLALDASGKTRAEAQNG
>Mature_374_residues
MRADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQMLVDAMATHDSIKQEMADNIE
LIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGEADSNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYK
VELQSMSDGDEAGIKSATYKITGLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVVDDNIDIEVNPADIRID
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRNAAINEAHENKGDAGWGNQI
RSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMAATLALDASGKTRAEAQNG

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=266, Percent_Identity=37.593984962406, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI34577120, Length=236, Percent_Identity=38.9830508474576, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=38.860103626943, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=356, Percent_Identity=53.6516853932584, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787462, Length=330, Percent_Identity=38.7878787878788, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=239, Percent_Identity=31.7991631799163, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=305, Percent_Identity=33.1147540983607, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI19921226, Length=227, Percent_Identity=38.7665198237885, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_ROSDO (Q165J6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683033.1
- ProteinModelPortal:   Q165J6
- SMR:   Q165J6
- STRING:   Q165J6
- GeneID:   4195589
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2819
- NMPDR:   fig|375451.6.peg.2615
- eggNOG:   COG1186
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- PhylomeDB:   Q165J6
- ProtClustDB:   PRK00578
- BioCyc:   RDEN375451:RD1_2819-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41476; Mature: 41476

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LVDAMATHDSIKQEMADNIELIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGE
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADSNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYKVELQSMSDGDEAGIKSATYK
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEE
ITGLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVVDDNIDIEVNPADIRID
EEECCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEEEEEECCCEEEEECCCEEEEE
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRR
ECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECHH
NAAINEAHENKGDAGWGNQIRSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMAATLA
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHE
LDASGKTRAEAQNG
ECCCCCCCCCCCCC
>Mature Secondary Structure
MRADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LVDAMATHDSIKQEMADNIELIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGE
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADSNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYKVELQSMSDGDEAGIKSATYK
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEE
ITGLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVVDDNIDIEVNPADIRID
EEECCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEEEEEECCCEEEEECCCEEEEE
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRR
ECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECHH
NAAINEAHENKGDAGWGNQIRSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMAATLA
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHE
LDASGKTRAEAQNG
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA