| Definition | Roseobacter denitrificans OCh 114, complete genome. |
|---|---|
| Accession | NC_008209 |
| Length | 4,133,097 |
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The map label for this gene is mrcA [H]
Identifier: 110680027
GI number: 110680027
Start: 2688606
End: 2691161
Strand: Reverse
Name: mrcA [H]
Synonym: RD1_2820
Alternate gene names: 110680027
Gene position: 2691161-2688606 (Counterclockwise)
Preceding gene: 110680028
Following gene: 110680026
Centisome position: 65.11
GC content: 59.74
Gene sequence:
>2556_bases GTGTTTCGGTTCATTCTGTCTTTTCTGGGTGGTATTTTTACGACCATAACCATGGGCTTGGGCATGGTTGTGCTGAGCAT TGGTGCGGTTTTCTGGGTCTATGGCAAGGATTTGCCCAGCCACGAATCACTCGCGCAATACGCCCCGCCCACCATCAGCC GGATTTATTCAGGCCAGGGGCAGATCATTGATGAATTCGCCAAGGAGAGGCGGCTGTTTACCCCGGCCGAGGACATCCCC GATCTGGTCAAACAGGCTTTCATCTCGGCAGAGGACAAGAATTTTTACCAGCACGACGGTTATGATCTGCGCGGTATCGG GGCGGCAGCGATTGATGCGGTGAAATCGCGCGGACAGGATGTGCGCGGCGCCTCGACGATCACGCAGCAGGTGATGAAGA ACTTCCTGCTCTCAGGTGACAGGCGTGCAGAACGCAAGATCAAGGAGATTATTCTGGCGTCCCGGCTGGAGGAAACGCTT GAGAAAGATCAGATCCTTGAGCTTTACCTGAACGAGATTTTTCTCGGCCAGAATTCTTACGGTGTCACCGCTGCGGCGCA GACATATTTCAACAAACCGCTGAGCGATCTGACGGCGCATGAGGCGGCGTTCCTCGCCTCCTTGCCCAAGGCGCCCAGCG ATTATCACCCCGTGCGCCAGAGGGAGCGGCTGCAGGCGCGTCGTGATTTCGTGCTCAAGGAGATGATGGAAAACGGCTAT ATCTCGCAGGCCGAATATGAAACCGAAGTCGCCGCGCCCCTGCGGTCGGTTCAAAACGGAGACTTCGAAAGCTTCCGCGC CGAGTTGCCACCGCGTGATTACTTCACGGATGAGATTCGCAGACAGTTGAGCGAAGATTTTGGTGAGGGCGAGTTCTTTA CCGGTGGGCTGAGCGTCCGCGCGACGATTGATCAGGATTTGCAACCGATCGCCGCCGAATCCCTGCGCCGCGCGTTGGAA GAATACGACCGCCGTCAGGGCATCTGGCGCGCACCGAAAACCCGGATAGACCCGGAACAGCTGGGCGATGAAACAGCATG GCGCACGGCCCTCGCGGCCGTTGAGATTGCGCGTGACATTGATCTGGACGGCCCGTGGTATCCGGCGGTGGTGTTATCCG TCGGCGAGACTTCGGCGCGCATTGGCATCGAAGGCGTTGTGGAGGACGAAGACGGGCATTTCATTCCCGCGCAAGACGTC ACCTGGGCGCGCAAGAAGCGCCGCGATGGCTCCCTGAGTGGCAAGGCGCAGCGTGCCGGTGATCTGCTTGAGGTGGGCGA TGTGGTCCATGTTCGCGCCATGACCTCGGATGAGGATGACAGTTTCATCCGCTGGACGCTGCGGCAGGTGCCCGAAGTGC AGGGCGGTTTTGTCGCCATGGACGTCAATTCAGGGCGCGTGATCTCGATGCAGGGCGGGTTTTCCTATCAGGACAGTGTG TTCAACCGCGCCACGCAGGCGCGCCGTCAACCGGGATCCAGTTTCAAACCCTTTGTCTATGCTTCCGCGCTCGACAGCGG CTATTCCCCGGCCACCATCGTCATTGACGCCCCGATCGAGATCAACACACCGCAAGGCCTGTGGCGTCCGCGCAATGCGT CGCGCGAATTTTATGGGCCAACTCCTTTGCGCACCGGCATCGAACGTTCCAGAAACCTGATGACCGTGCGACTGGCACAA GAGGTCGGCATGGATGTTGTGGCCGATTATGCCGAACGGTTTGGCGTTTATGACAACATGGGCACCTTTCTGGCCAATGC GCTGGGGTCCGAGGAAACCACGCTTTACCGGATGGTTGCCGCCTATGCGATGTTTGCCAACGGCGGCGAACGGGTGCAGC CGACGCTGGTGGACCGGGTTCAGGACCGCTACGGGCGCACGATTTACCGCCATGACGAACGCTATTGTTTCGATTGTGAA CTGGCCAGCCTTGATCCCGGCCTTGGCCCACGGATCATCTCGAACCGGGAACGCGTGATGGACCCGATCACCGCCTATCA GCTGACCTCGATGATGAAAGGGGTTGTGGACCGCGGGACCGCTGCGCGCACGGTCAACCTTCCTGTGCCCACCGCCGGAA AAACCGGCACAACCAATGACGCCAAAGACGTCTGGTTCGTCGGGTTCACCAGCAACATCGTTGCGGGCTGCTACATCGGG TATGATCAACCACGCTCGCTGGGGCGCGGCGCGTCGGGGGGCGGCATGTGCGGCCCGGTGTTCCAGCGGTTCATGGAAGA AGCCACGCAAAAATATGGCGGCGGCCCGTTTGAGGTGCCAAGCGGCGGGCGGTTTATCAAGATCGACCGTTTTACCGGTG CGCGCCTGAGCGATGACGCCACCGGCGATGCGGTTGTGGCAGAGTATTTCCGCAGTGGCGAAGAGCCGTTGTTCGGCGTC ATGTTCGACGGTGGCTTTGCCATGGGTCAGGATTTGCCGCTGGTTCAGGAAGCGCAGCCCGCACAACGCCAGCAGGTGAC GACCTCGACCGGGGAAACGGTTGAGGTTGGCCCCAATGCCAATTTCGGCACGCTCAGTTCCGGCGGTCTATACTGA
Upstream 100 bases:
>100_bases GGCAGTAGCGATCACGTGCTTATGACGTCGCGCTCTTTTGACCACGGCCAAAGAAACACATATACCATCCCTGTAGCGTC AACAAAGGTGCGTGCTTTTC
Downstream 100 bases:
>100_bases ACACGGGTGTGTAATCGCCCCGGGTGCCTTGCCGCTGCTGTGGTGCTGGTCTATCAGGATGTAACACTTTTTTGCCGAGG CTGACATGCGCGCAGACGTC
Product: penicillin-binding protein 1A
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 851; Mature: 851
Protein sequence:
>851_residues MFRFILSFLGGIFTTITMGLGMVVLSIGAVFWVYGKDLPSHESLAQYAPPTISRIYSGQGQIIDEFAKERRLFTPAEDIP DLVKQAFISAEDKNFYQHDGYDLRGIGAAAIDAVKSRGQDVRGASTITQQVMKNFLLSGDRRAERKIKEIILASRLEETL EKDQILELYLNEIFLGQNSYGVTAAAQTYFNKPLSDLTAHEAAFLASLPKAPSDYHPVRQRERLQARRDFVLKEMMENGY ISQAEYETEVAAPLRSVQNGDFESFRAELPPRDYFTDEIRRQLSEDFGEGEFFTGGLSVRATIDQDLQPIAAESLRRALE EYDRRQGIWRAPKTRIDPEQLGDETAWRTALAAVEIARDIDLDGPWYPAVVLSVGETSARIGIEGVVEDEDGHFIPAQDV TWARKKRRDGSLSGKAQRAGDLLEVGDVVHVRAMTSDEDDSFIRWTLRQVPEVQGGFVAMDVNSGRVISMQGGFSYQDSV FNRATQARRQPGSSFKPFVYASALDSGYSPATIVIDAPIEINTPQGLWRPRNASREFYGPTPLRTGIERSRNLMTVRLAQ EVGMDVVADYAERFGVYDNMGTFLANALGSEETTLYRMVAAYAMFANGGERVQPTLVDRVQDRYGRTIYRHDERYCFDCE LASLDPGLGPRIISNRERVMDPITAYQLTSMMKGVVDRGTAARTVNLPVPTAGKTGTTNDAKDVWFVGFTSNIVAGCYIG YDQPRSLGRGASGGGMCGPVFQRFMEEATQKYGGGPFEVPSGGRFIKIDRFTGARLSDDATGDAVVAEYFRSGEEPLFGV MFDGGFAMGQDLPLVQEAQPAQRQQVTTSTGETVEVGPNANFGTLSSGGLY
Sequences:
>Translated_851_residues MFRFILSFLGGIFTTITMGLGMVVLSIGAVFWVYGKDLPSHESLAQYAPPTISRIYSGQGQIIDEFAKERRLFTPAEDIP DLVKQAFISAEDKNFYQHDGYDLRGIGAAAIDAVKSRGQDVRGASTITQQVMKNFLLSGDRRAERKIKEIILASRLEETL EKDQILELYLNEIFLGQNSYGVTAAAQTYFNKPLSDLTAHEAAFLASLPKAPSDYHPVRQRERLQARRDFVLKEMMENGY ISQAEYETEVAAPLRSVQNGDFESFRAELPPRDYFTDEIRRQLSEDFGEGEFFTGGLSVRATIDQDLQPIAAESLRRALE EYDRRQGIWRAPKTRIDPEQLGDETAWRTALAAVEIARDIDLDGPWYPAVVLSVGETSARIGIEGVVEDEDGHFIPAQDV TWARKKRRDGSLSGKAQRAGDLLEVGDVVHVRAMTSDEDDSFIRWTLRQVPEVQGGFVAMDVNSGRVISMQGGFSYQDSV FNRATQARRQPGSSFKPFVYASALDSGYSPATIVIDAPIEINTPQGLWRPRNASREFYGPTPLRTGIERSRNLMTVRLAQ EVGMDVVADYAERFGVYDNMGTFLANALGSEETTLYRMVAAYAMFANGGERVQPTLVDRVQDRYGRTIYRHDERYCFDCE LASLDPGLGPRIISNRERVMDPITAYQLTSMMKGVVDRGTAARTVNLPVPTAGKTGTTNDAKDVWFVGFTSNIVAGCYIG YDQPRSLGRGASGGGMCGPVFQRFMEEATQKYGGGPFEVPSGGRFIKIDRFTGARLSDDATGDAVVAEYFRSGEEPLFGV MFDGGFAMGQDLPLVQEAQPAQRQQVTTSTGETVEVGPNANFGTLSSGGLY >Mature_851_residues MFRFILSFLGGIFTTITMGLGMVVLSIGAVFWVYGKDLPSHESLAQYAPPTISRIYSGQGQIIDEFAKERRLFTPAEDIP DLVKQAFISAEDKNFYQHDGYDLRGIGAAAIDAVKSRGQDVRGASTITQQVMKNFLLSGDRRAERKIKEIILASRLEETL EKDQILELYLNEIFLGQNSYGVTAAAQTYFNKPLSDLTAHEAAFLASLPKAPSDYHPVRQRERLQARRDFVLKEMMENGY ISQAEYETEVAAPLRSVQNGDFESFRAELPPRDYFTDEIRRQLSEDFGEGEFFTGGLSVRATIDQDLQPIAAESLRRALE EYDRRQGIWRAPKTRIDPEQLGDETAWRTALAAVEIARDIDLDGPWYPAVVLSVGETSARIGIEGVVEDEDGHFIPAQDV TWARKKRRDGSLSGKAQRAGDLLEVGDVVHVRAMTSDEDDSFIRWTLRQVPEVQGGFVAMDVNSGRVISMQGGFSYQDSV FNRATQARRQPGSSFKPFVYASALDSGYSPATIVIDAPIEINTPQGLWRPRNASREFYGPTPLRTGIERSRNLMTVRLAQ EVGMDVVADYAERFGVYDNMGTFLANALGSEETTLYRMVAAYAMFANGGERVQPTLVDRVQDRYGRTIYRHDERYCFDCE LASLDPGLGPRIISNRERVMDPITAYQLTSMMKGVVDRGTAARTVNLPVPTAGKTGTTNDAKDVWFVGFTSNIVAGCYIG YDQPRSLGRGASGGGMCGPVFQRFMEEATQKYGGGPFEVPSGGRFIKIDRFTGARLSDDATGDAVVAEYFRSGEEPLFGV MFDGGFAMGQDLPLVQEAQPAQRQQVTTSTGETVEVGPNANFGTLSSGGLY
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=859, Percent_Identity=30.9662398137369, Blast_Score=375, Evalue=1e-105, Organism=Escherichia coli, GI1786343, Length=250, Percent_Identity=34, Blast_Score=126, Evalue=6e-30, Organism=Escherichia coli, GI1788867, Length=178, Percent_Identity=34.8314606741573, Blast_Score=94, Evalue=3e-20, Organism=Escherichia coli, GI1789601, Length=168, Percent_Identity=36.3095238095238, Blast_Score=94, Evalue=3e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.-
Molecular weight: Translated: 94183; Mature: 94183
Theoretical pI: Translated: 4.77; Mature: 4.77
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFRFILSFLGGIFTTITMGLGMVVLSIGAVFWVYGKDLPSHESLAQYAPPTISRIYSGQG CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHCCCC QIIDEFAKERRLFTPAEDIPDLVKQAFISAEDKNFYQHDGYDLRGIGAAAIDAVKSRGQD HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCCC VRGASTITQQVMKNFLLSGDRRAERKIKEIILASRLEETLEKDQILELYLNEIFLGQNSY CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC GVTAAAQTYFNKPLSDLTAHEAAFLASLPKAPSDYHPVRQRERLQARRDFVLKEMMENGY CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC ISQAEYETEVAAPLRSVQNGDFESFRAELPPRDYFTDEIRRQLSEDFGEGEFFTGGLSVR CCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEE ATIDQDLQPIAAESLRRALEEYDRRQGIWRAPKTRIDPEQLGDETAWRTALAAVEIARDI EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHC DLDGPWYPAVVLSVGETSARIGIEGVVEDEDGHFIPAQDVTWARKKRRDGSLSGKAQRAG CCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEECCCCCHHHHHHCCCCCCCCCHHHCC DLLEVGDVVHVRAMTSDEDDSFIRWTLRQVPEVQGGFVAMDVNSGRVISMQGGFSYQDSV CHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCHHHHH FNRATQARRQPGSSFKPFVYASALDSGYSPATIVIDAPIEINTPQGLWRPRNASREFYGP HHHHHHHHHCCCCCCCCEEEEHHHCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCC TPLRTGIERSRNLMTVRLAQEVGMDVVADYAERFGVYDNMGTFLANALGSEETTLYRMVA CHHHHHHHHHCCEEEEEEHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH AYAMFANGGERVQPTLVDRVQDRYGRTIYRHDERYCFDCELASLDPGLGPRIISNRERVM HHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCCHHHCCHHHHH DPITAYQLTSMMKGVVDRGTAARTVNLPVPTAGKTGTTNDAKDVWFVGFTSNIVAGCYIG HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHEEEEEC YDQPRSLGRGASGGGMCGPVFQRFMEEATQKYGGGPFEVPSGGRFIKIDRFTGARLSDDA CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCC TGDAVVAEYFRSGEEPLFGVMFDGGFAMGQDLPLVQEAQPAQRQQVTTSTGETVEVGPNA CCHHHHHHHHHCCCCCEEEEEECCCEECCCCCCCHHCCCCHHHHHHCCCCCCEEEECCCC NFGTLSSGGLY CCCCCCCCCCC >Mature Secondary Structure MFRFILSFLGGIFTTITMGLGMVVLSIGAVFWVYGKDLPSHESLAQYAPPTISRIYSGQG CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHCCCC QIIDEFAKERRLFTPAEDIPDLVKQAFISAEDKNFYQHDGYDLRGIGAAAIDAVKSRGQD HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCCC VRGASTITQQVMKNFLLSGDRRAERKIKEIILASRLEETLEKDQILELYLNEIFLGQNSY CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC GVTAAAQTYFNKPLSDLTAHEAAFLASLPKAPSDYHPVRQRERLQARRDFVLKEMMENGY CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC ISQAEYETEVAAPLRSVQNGDFESFRAELPPRDYFTDEIRRQLSEDFGEGEFFTGGLSVR CCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEE ATIDQDLQPIAAESLRRALEEYDRRQGIWRAPKTRIDPEQLGDETAWRTALAAVEIARDI EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHC DLDGPWYPAVVLSVGETSARIGIEGVVEDEDGHFIPAQDVTWARKKRRDGSLSGKAQRAG CCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEECCCCCHHHHHHCCCCCCCCCHHHCC DLLEVGDVVHVRAMTSDEDDSFIRWTLRQVPEVQGGFVAMDVNSGRVISMQGGFSYQDSV CHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCHHHHH FNRATQARRQPGSSFKPFVYASALDSGYSPATIVIDAPIEINTPQGLWRPRNASREFYGP HHHHHHHHHCCCCCCCCEEEEHHHCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCC TPLRTGIERSRNLMTVRLAQEVGMDVVADYAERFGVYDNMGTFLANALGSEETTLYRMVA CHHHHHHHHHCCEEEEEEHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH AYAMFANGGERVQPTLVDRVQDRYGRTIYRHDERYCFDCELASLDPGLGPRIISNRERVM HHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCCHHHCCHHHHH DPITAYQLTSMMKGVVDRGTAARTVNLPVPTAGKTGTTNDAKDVWFVGFTSNIVAGCYIG HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHEEEEEC YDQPRSLGRGASGGGMCGPVFQRFMEEATQKYGGGPFEVPSGGRFIKIDRFTGARLSDDA CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCC TGDAVVAEYFRSGEEPLFGVMFDGGFAMGQDLPLVQEAQPAQRQQVTTSTGETVEVGPNA CCHHHHHHHHHCCCCCEEEEEECCCEECCCCCCCHHCCCCHHHHHHCCCCCCEEEECCCC NFGTLSSGGLY CCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA