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The map label for this gene is potD-B [H]

Identifier: 110679621

GI number: 110679621

Start: 2261969

End: 2263108

Strand: Reverse

Name: potD-B [H]

Synonym: RD1_2369

Alternate gene names: 110679621

Gene position: 2263108-2261969 (Counterclockwise)

Preceding gene: 110679622

Following gene: 110679620

Centisome position: 54.76

GC content: 57.11

Gene sequence:

>1140_bases
ATGACTAAGACAACACCAGGATCATTCATGAGCGCACCAAGCCGGCGCCAATTGCTCAAGGCGGGCGGTGCCGCTGTGCT
GAGCACGCCCTTTGTGAGCCGTGCATGGGCCTCAACCACCGAGATCAACATGCTGGCGTGGTACGGACACGGCGAGCCGG
ATATCGTTGCGGAATACGAAGCTGCCAACAACGTGAAATTCGTCCCCAAATACTATGCAGGGGGTGACAACATGCTGGCG
CTAATCGCGCAGTCCCCGCCGGGCACTTACGATCTGATCCTGTCGGACGCGGAGTTTGTGCAGCAGCTCAACCTCGCGGA
ATATATCGAAGAGATGAACCCCGGCGACTATCCGCTGGACGATATGCTGCATGAGGATTTCACACAATTTCCCGGCCATT
GGGCCGATGGCAAGATGTATTCCATGATGCTGCGTGGCGGCCATCTGGGTGTGTCTTACAACACAAATTCTGTGTCTGCC
GAAGAAGCGGAAAGCTACGCCTGCTTCTGGAAACCGGAGCTTGAAGGCAAGGTCGGTCATTTTGACTGGCACCTGCCGAC
GCTGGGCATGATGAGCCTTTATGACGGGAATGGCGCAAACCTCTGGAACCTTGACGACGACCAGTGGGACAAGGTGCAGG
AAACCACCATGAGCCTGCGCAAACAGGTCGGCGGTTTCTTTGATTATGGCGGCACGTTCAACGGCCTCAAGAACGGTGAG
ATGCAGGCGATGGTCGGCATCGGTGACTGGATCACGGGCGTTCTGGAAAAAGACGGCGCGCCGGTCGCATCTGTGATCCC
GAAAGAGGGCGGCATCCAGTTCACCGAGAGCTATTCCATCGGCAAGGGCAGCGAAAAAGTGGAAGAGGCCAGGAAATTCA
TCCAGTATATGATGTCCCCGGCAGGTCAGGTGAAATCGGCGCAAATGGCGGCCTATCCGGGGTTTTGCGTAACCAAGGCA
GGTCGCGCCGCCCTGATTGAAGCGGACCCGGCCGAGGCACAACGCTCGCATCAGATTGACGGTGATCCACAAGATCCGAT
CACCCTGATCAACGAAGGGCGCATCCACTACCGCGATATCCCGCAGCAACAGTCGCTGGAGGATTGGAACGACTTCTGGT
CCGAGTACAAAAGCGCCTGA

Upstream 100 bases:

>100_bases
TTTTACAATAGCATTCTTTCAAACACCGACCGAACCTGAAGTTCGGCAAGACAATAATGGCTCTTTTGTTGAAAAAAGAG
CATCCAACCGGGAGACGACG

Downstream 100 bases:

>100_bases
TCAACCGCTGAACGACATCAAAGAGGGCGGCCCACCGGTCGCCCCCCTTTCCCAAAGAATGCGAAAGCCCCATGTCAACC
ACCCCTGAACCGCGACCGAA

Product: spermidine/putrescine ABC transporter, periplasmic binding protein, putative

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTKTTPGSFMSAPSRRQLLKAGGAAVLSTPFVSRAWASTTEINMLAWYGHGEPDIVAEYEAANNVKFVPKYYAGGDNMLA
LIAQSPPGTYDLILSDAEFVQQLNLAEYIEEMNPGDYPLDDMLHEDFTQFPGHWADGKMYSMMLRGGHLGVSYNTNSVSA
EEAESYACFWKPELEGKVGHFDWHLPTLGMMSLYDGNGANLWNLDDDQWDKVQETTMSLRKQVGGFFDYGGTFNGLKNGE
MQAMVGIGDWITGVLEKDGAPVASVIPKEGGIQFTESYSIGKGSEKVEEARKFIQYMMSPAGQVKSAQMAAYPGFCVTKA
GRAALIEADPAEAQRSHQIDGDPQDPITLINEGRIHYRDIPQQQSLEDWNDFWSEYKSA

Sequences:

>Translated_379_residues
MTKTTPGSFMSAPSRRQLLKAGGAAVLSTPFVSRAWASTTEINMLAWYGHGEPDIVAEYEAANNVKFVPKYYAGGDNMLA
LIAQSPPGTYDLILSDAEFVQQLNLAEYIEEMNPGDYPLDDMLHEDFTQFPGHWADGKMYSMMLRGGHLGVSYNTNSVSA
EEAESYACFWKPELEGKVGHFDWHLPTLGMMSLYDGNGANLWNLDDDQWDKVQETTMSLRKQVGGFFDYGGTFNGLKNGE
MQAMVGIGDWITGVLEKDGAPVASVIPKEGGIQFTESYSIGKGSEKVEEARKFIQYMMSPAGQVKSAQMAAYPGFCVTKA
GRAALIEADPAEAQRSHQIDGDPQDPITLINEGRIHYRDIPQQQSLEDWNDFWSEYKSA
>Mature_378_residues
TKTTPGSFMSAPSRRQLLKAGGAAVLSTPFVSRAWASTTEINMLAWYGHGEPDIVAEYEAANNVKFVPKYYAGGDNMLAL
IAQSPPGTYDLILSDAEFVQQLNLAEYIEEMNPGDYPLDDMLHEDFTQFPGHWADGKMYSMMLRGGHLGVSYNTNSVSAE
EAESYACFWKPELEGKVGHFDWHLPTLGMMSLYDGNGANLWNLDDDQWDKVQETTMSLRKQVGGFFDYGGTFNGLKNGEM
QAMVGIGDWITGVLEKDGAPVASVIPKEGGIQFTESYSIGKGSEKVEEARKFIQYMMSPAGQVKSAQMAAYPGFCVTKAG
RAALIEADPAEAQRSHQIDGDPQDPITLINEGRIHYRDIPQQQSLEDWNDFWSEYKSA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=277, Percent_Identity=22.7436823104693, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41918; Mature: 41787

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTTPGSFMSAPSRRQLLKAGGAAVLSTPFVSRAWASTTEINMLAWYGHGEPDIVAEYE
CCCCCCCHHHCCCHHHHHHHHCCCCEEECCHHHHHHCCCCEEEEEEEECCCCCCEEEEEC
AANNVKFVPKYYAGGDNMLALIAQSPPGTYDLILSDAEFVQQLNLAEYIEEMNPGDYPLD
CCCCCEECEEEECCCCCEEEEEECCCCCCEEEEECCHHHHHHCCHHHHHHHCCCCCCCHH
DMLHEDFTQFPGHWADGKMYSMMLRGGHLGVSYNTNSVSAEEAESYACFWKPELEGKVGH
HHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHCCEEEEECCCCCCCCCC
FDWHLPTLGMMSLYDGNGANLWNLDDDQWDKVQETTMSLRKQVGGFFDYGGTFNGLKNGE
EEECCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCC
MQAMVGIGDWITGVLEKDGAPVASVIPKEGGIQFTESYSIGKGSEKVEEARKFIQYMMSP
EEEEEECHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCC
AGQVKSAQMAAYPGFCVTKAGRAALIEADPAEAQRSHQIDGDPQDPITLINEGRIHYRDI
CCCCCCCHHHCCCCEEEECCCCEEEEECCCHHHHHCCCCCCCCCCCEEEEECCEEEEECC
PQQQSLEDWNDFWSEYKSA
CCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTTPGSFMSAPSRRQLLKAGGAAVLSTPFVSRAWASTTEINMLAWYGHGEPDIVAEYE
CCCCCCHHHCCCHHHHHHHHCCCCEEECCHHHHHHCCCCEEEEEEEECCCCCCEEEEEC
AANNVKFVPKYYAGGDNMLALIAQSPPGTYDLILSDAEFVQQLNLAEYIEEMNPGDYPLD
CCCCCEECEEEECCCCCEEEEEECCCCCCEEEEECCHHHHHHCCHHHHHHHCCCCCCCHH
DMLHEDFTQFPGHWADGKMYSMMLRGGHLGVSYNTNSVSAEEAESYACFWKPELEGKVGH
HHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHCCEEEEECCCCCCCCCC
FDWHLPTLGMMSLYDGNGANLWNLDDDQWDKVQETTMSLRKQVGGFFDYGGTFNGLKNGE
EEECCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCC
MQAMVGIGDWITGVLEKDGAPVASVIPKEGGIQFTESYSIGKGSEKVEEARKFIQYMMSP
EEEEEECHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCC
AGQVKSAQMAAYPGFCVTKAGRAALIEADPAEAQRSHQIDGDPQDPITLINEGRIHYRDI
CCCCCCCHHHCCCCEEEECCCCEEEEECCCHHHHHCCCCCCCCCCCEEEEECCEEEEECC
PQQQSLEDWNDFWSEYKSA
CCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]