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The map label for this gene is potH [H]

Identifier: 110679620

GI number: 110679620

Start: 2261034

End: 2261897

Strand: Reverse

Name: potH [H]

Synonym: RD1_2368

Alternate gene names: 110679620

Gene position: 2261897-2261034 (Counterclockwise)

Preceding gene: 110679621

Following gene: 110679619

Centisome position: 54.73

GC content: 56.02

Gene sequence:

>864_bases
ATGTCAACCACCCCTGAACCGCGACCGAATATCTATGCGCTGACCTGGCGTCTGCCGATCATCTTCTGGCAATTGGTCTT
CTTTGTCGGGCCTGTCCTGTTCATGGTCGCAATGTCGTTTTTCCTCGTGAAAAACTACCGCATGACCGAAGCGTTCGAGT
TCGTTAACTGGGGGCGCATGCTGACGCGCGGCTTCTTTTGGGACAGCTACTGGTACACGGTTTTCATTGCCACCGTGTCG
ACCGTCTGCGCGATGTGTATCGCATTCCCCGCAGCCTTTGCCTTGGCCTTTCGCGCCTCTGAAAGTACCCGCCGTTGGGC
GATCTTCCTGCTGATCATTCCCTTTTTCACCAGCTATCTGGTGCGCACCTTTTCGTGGTTCGTGATCCTGTCTGAAAGTG
GCGTGGTCAATGCCATTTTGGGCTATGTCGGTCTTGGACCCTATACAATGCTGAACACGAATTTTGGCACATTGGTCGGA
TACATGACCCTGACCCTGCCCCTTGTCGTGATCCTGCAGACCGTGACCATGGCCAATATCGACAAGAACCTGATCGAGGC
GGCCCGCAACCTCGGCTGCTCGCCGCTCAGAACGATCTGGCAGGTGATCATTCCGCTTGCCAAGACCGGCCTGATCATTG
CCGCGATCTTTTGCTTCATCCTGTCGTTTGGTGATTTCGTGGCCCCGTTTTACCTTGGCGGATCACAGGAACCGACCCTG
CCGATCCTGATCCTTGACACCACCAAATCCGGGCAGCAATGGCCCCGCGCCGCTGTGGTCGCCATCATGATGATGCTCAC
GTTGTTCGCCATTGCCTTCACCGGCATCGCCTTGGCCTACCGCAAGGGAAAGGGCGCGAAATGA

Upstream 100 bases:

>100_bases
ACTTCTGGTCCGAGTACAAAAGCGCCTGATCAACCGCTGAACGACATCAAAGAGGGCGGCCCACCGGTCGCCCCCCTTTC
CCAAAGAATGCGAAAGCCCC

Downstream 100 bases:

>100_bases
AACAAAGCCGGTCCCTGAACTTTGCCCTCGCCCTTTATGTCGCGCTGATCTTTGTCTTCGTTTTCGCGCCGATCGTGTTC
AGCATGATCTTCTCCTTCAA

Product: putrescine ABC transporter, permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSTTPEPRPNIYALTWRLPIIFWQLVFFVGPVLFMVAMSFFLVKNYRMTEAFEFVNWGRMLTRGFFWDSYWYTVFIATVS
TVCAMCIAFPAAFALAFRASESTRRWAIFLLIIPFFTSYLVRTFSWFVILSESGVVNAILGYVGLGPYTMLNTNFGTLVG
YMTLTLPLVVILQTVTMANIDKNLIEAARNLGCSPLRTIWQVIIPLAKTGLIIAAIFCFILSFGDFVAPFYLGGSQEPTL
PILILDTTKSGQQWPRAAVVAIMMMLTLFAIAFTGIALAYRKGKGAK

Sequences:

>Translated_287_residues
MSTTPEPRPNIYALTWRLPIIFWQLVFFVGPVLFMVAMSFFLVKNYRMTEAFEFVNWGRMLTRGFFWDSYWYTVFIATVS
TVCAMCIAFPAAFALAFRASESTRRWAIFLLIIPFFTSYLVRTFSWFVILSESGVVNAILGYVGLGPYTMLNTNFGTLVG
YMTLTLPLVVILQTVTMANIDKNLIEAARNLGCSPLRTIWQVIIPLAKTGLIIAAIFCFILSFGDFVAPFYLGGSQEPTL
PILILDTTKSGQQWPRAAVVAIMMMLTLFAIAFTGIALAYRKGKGAK
>Mature_286_residues
STTPEPRPNIYALTWRLPIIFWQLVFFVGPVLFMVAMSFFLVKNYRMTEAFEFVNWGRMLTRGFFWDSYWYTVFIATVST
VCAMCIAFPAAFALAFRASESTRRWAIFLLIIPFFTSYLVRTFSWFVILSESGVVNAILGYVGLGPYTMLNTNFGTLVGY
MTLTLPLVVILQTVTMANIDKNLIEAARNLGCSPLRTIWQVIIPLAKTGLIIAAIFCFILSFGDFVAPFYLGGSQEPTLP
ILILDTTKSGQQWPRAAVVAIMMMLTLFAIAFTGIALAYRKGKGAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=215, Percent_Identity=34.4186046511628, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787369, Length=246, Percent_Identity=27.2357723577236, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787713, Length=225, Percent_Identity=24, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32226; Mature: 32095

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STTPEPRPNIYALTWRLPIIFWQLVFFVGPVLFMVAMSFFLVKNYRMTEAFEFVNWGRM
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTRGFFWDSYWYTVFIATVSTVCAMCIAFPAAFALAFRASESTRRWAIFLLIIPFFTSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VRTFSWFVILSESGVVNAILGYVGLGPYTMLNTNFGTLVGYMTLTLPLVVILQTVTMANI
HHHHHHHHEECCCCHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DKNLIEAARNLGCSPLRTIWQVIIPLAKTGLIIAAIFCFILSFGDFVAPFYLGGSQEPTL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
PILILDTTKSGQQWPRAAVVAIMMMLTLFAIAFTGIALAYRKGKGAK
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]