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The map label for this gene is stcD [H]

Identifier: 110679463

GI number: 110679463

Start: 2086478

End: 2088523

Strand: Reverse

Name: stcD [H]

Synonym: RD1_2189

Alternate gene names: 110679463

Gene position: 2088523-2086478 (Counterclockwise)

Preceding gene: 110679464

Following gene: 110679455

Centisome position: 50.53

GC content: 60.07

Gene sequence:

>2046_bases
ATGTCAAAAGATCCCTTGATGCAACCCTTTCAACTCAAGCATCTGACGTTGAAGAACCGCATCATGACCACCAGCCATGA
ACCTGCCTATCCCGAAGACGGCATGCCCAAGGAACGCTACCGCGCCTATCACGAAGAGCGCGCCAAGGCCGGGCTTGCAC
TTGCGATGACCGCAGGTTCGGCGGCGGTGTCCAAAGACAGCCCGCCGGTGTTCAACAACGTGCTGGCCTATAAGGATGAG
GTGGTGCCATGGATCACCGATCTGACCGATGCCTGCCATGCGCATGGCTGTGCGGTGATGATCCAGTTGACGCATCTGGG
GCGGCGCACGACGTGGAACAAAGGCGACTGGTTGCCCTCCCTGTCTTCCTCGCGCCACCGCGAACCGGCGCATCGCGCCT
TTCCCAAACTGATCGAAGACTGGGATATTGACCGGGTGATCACCGATTTCGCCGACGCAGCCGAGCGGATGCAGGCCGGT
GGCATGGATGGGATCGAATTGCAGGTTTACGGCCATCTGCTGGACCAGTTCTGGTCACCGCTTACGAACGATCTGTCCGG
TCCTTACGGTGCCGATACTTTGGAAAACCGGATGCGGTTTCCGCTGGATGTCTTGGGGGCCATTCGCAAACGCGTGGGCG
AGGATTTCATCATCGGCTTTCGCTTTACCGCCGATGAGGTGCAAAAAGGCGGGATCACGCCCGAAGAGGGCCTGCGCATT
TCAAAAATCCTCGCCGATGATGGGCGTCTCGATTTCCTGAATGTGATCCGGGGGCGCATCCATACGGACCCTGCGATGGT
CGACGTGATCCCGGTGCAGGGCATGAGCAATGCGCCACATCTTGATTTCGCCGGGGCGGTCAAAAAGGCAACCGGCCTGC
CCACATTCCACGCCGCCAAAATCCCCGATGTGGCGACTGCGCGACATGCCGTGGCAAACGGGCTGCTCGATATGGTCGGG
ATGACGCGGGCGCATATGGCTGATCCGCATATTGTGCAGAAAATCCGCGAGGGGCGCGAGGACGACATACGCCCTTGTGT
CGGGGCCACCTTTTGTCTGGATCGGATTTATCAGGGCGGCGAGGCGCTGTGCATTCACAACGCGGCCACGGGGCGCGAAC
TGTCCATGCCGCACGTCATAGCCCCGGCCCCTGCCCCACGCCATGTTGTGATTGTCGGCGCAGGTCCGGCGGGGCTTGAG
GCCGCCCGTGTTGCCGCCGAGCGCGGGCACGTTGTCACGGTCTTTGAGGCGCAAGCCGATCCCGGTGGGCAAATCCGGCT
GACCGTCCAGAACCCGCGCCGCCGCGATATGATTTCAATCATCGACTGGCGCATGGCCCAATGTGCCGCGCGCGATGTGC
GTTTTCATTTCAACACATGGGCCGAACCTGAAACAATCACCGACCTGAATCCGGATGTGGTGATCGTGGCGACGGGCGGC
CTGCCAAACACCGAACTTTTCGAGGCTGGCCGCGACACGCCCCATGTGATCAGCAGTTGGGAGATGATCGCAGGCGATGT
CAAACCGGCAGATCACGTGTTGATCTATGATGAAAGCGGCGACCATCCGGGGCTGCAAGCCGCTGAGACGGCTGCCCTGG
CCGGTGCAAAAGTCGAGGTGATGACGCCTGATCGCGTATTTGCGCCCGACATCATGGCGATGAACCTGACACCTTACATG
CGCGCGTTGCAGGACAAGGACGTCACCTTTACCGTGACGCGCCGCCTGCTGGATGTGGCGCGCAATGGCAACAAGTTACG
TGCCACAATCGGGACGGATTACAGCGATCACAGCTATCAGACCGATTACGACCAGATCGTTGTGAACTATGGGACCTTCC
CGCTGGATGAGCTTTACCACGCGCTCAAACCGCTCAGCAGCAACCTTGGCGCTGTGGATCATGACGCGCTGATCGCGGGC
GAAGTCCAGAATGTGAACACCAACTCCGATGGCGCGTTTCAGCTTTTCCGCATCGGGGATGCGGTTTCGGCCCGCAACAC
ACATGCGGCGATTTACGACGCCCTGAGGCTGGTCAAGGACTTGTGA

Upstream 100 bases:

>100_bases
CCGGCGTTTTTGATCTGGCAAAGCCCTAGGATATGCGGCTTACTGCGCGTTGAAGCGTCGCCGAGACGGGACGCAACAGG
ACAGGTAGAACGTGCAGCCG

Downstream 100 bases:

>100_bases
GCACGCATCCGGCGACCCCGACGCATACTCAGTCGCGCTTTGAGGACGCATAGGCTTGCGACGCCGCATAGTAGTCGTCA
AACCCGATCCGGCTGCGCAA

Product: oxidoreductase, putative

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MSKDPLMQPFQLKHLTLKNRIMTTSHEPAYPEDGMPKERYRAYHEERAKAGLALAMTAGSAAVSKDSPPVFNNVLAYKDE
VVPWITDLTDACHAHGCAVMIQLTHLGRRTTWNKGDWLPSLSSSRHREPAHRAFPKLIEDWDIDRVITDFADAAERMQAG
GMDGIELQVYGHLLDQFWSPLTNDLSGPYGADTLENRMRFPLDVLGAIRKRVGEDFIIGFRFTADEVQKGGITPEEGLRI
SKILADDGRLDFLNVIRGRIHTDPAMVDVIPVQGMSNAPHLDFAGAVKKATGLPTFHAAKIPDVATARHAVANGLLDMVG
MTRAHMADPHIVQKIREGREDDIRPCVGATFCLDRIYQGGEALCIHNAATGRELSMPHVIAPAPAPRHVVIVGAGPAGLE
AARVAAERGHVVTVFEAQADPGGQIRLTVQNPRRRDMISIIDWRMAQCAARDVRFHFNTWAEPETITDLNPDVVIVATGG
LPNTELFEAGRDTPHVISSWEMIAGDVKPADHVLIYDESGDHPGLQAAETAALAGAKVEVMTPDRVFAPDIMAMNLTPYM
RALQDKDVTFTVTRRLLDVARNGNKLRATIGTDYSDHSYQTDYDQIVVNYGTFPLDELYHALKPLSSNLGAVDHDALIAG
EVQNVNTNSDGAFQLFRIGDAVSARNTHAAIYDALRLVKDL

Sequences:

>Translated_681_residues
MSKDPLMQPFQLKHLTLKNRIMTTSHEPAYPEDGMPKERYRAYHEERAKAGLALAMTAGSAAVSKDSPPVFNNVLAYKDE
VVPWITDLTDACHAHGCAVMIQLTHLGRRTTWNKGDWLPSLSSSRHREPAHRAFPKLIEDWDIDRVITDFADAAERMQAG
GMDGIELQVYGHLLDQFWSPLTNDLSGPYGADTLENRMRFPLDVLGAIRKRVGEDFIIGFRFTADEVQKGGITPEEGLRI
SKILADDGRLDFLNVIRGRIHTDPAMVDVIPVQGMSNAPHLDFAGAVKKATGLPTFHAAKIPDVATARHAVANGLLDMVG
MTRAHMADPHIVQKIREGREDDIRPCVGATFCLDRIYQGGEALCIHNAATGRELSMPHVIAPAPAPRHVVIVGAGPAGLE
AARVAAERGHVVTVFEAQADPGGQIRLTVQNPRRRDMISIIDWRMAQCAARDVRFHFNTWAEPETITDLNPDVVIVATGG
LPNTELFEAGRDTPHVISSWEMIAGDVKPADHVLIYDESGDHPGLQAAETAALAGAKVEVMTPDRVFAPDIMAMNLTPYM
RALQDKDVTFTVTRRLLDVARNGNKLRATIGTDYSDHSYQTDYDQIVVNYGTFPLDELYHALKPLSSNLGAVDHDALIAG
EVQNVNTNSDGAFQLFRIGDAVSARNTHAAIYDALRLVKDL
>Mature_680_residues
SKDPLMQPFQLKHLTLKNRIMTTSHEPAYPEDGMPKERYRAYHEERAKAGLALAMTAGSAAVSKDSPPVFNNVLAYKDEV
VPWITDLTDACHAHGCAVMIQLTHLGRRTTWNKGDWLPSLSSSRHREPAHRAFPKLIEDWDIDRVITDFADAAERMQAGG
MDGIELQVYGHLLDQFWSPLTNDLSGPYGADTLENRMRFPLDVLGAIRKRVGEDFIIGFRFTADEVQKGGITPEEGLRIS
KILADDGRLDFLNVIRGRIHTDPAMVDVIPVQGMSNAPHLDFAGAVKKATGLPTFHAAKIPDVATARHAVANGLLDMVGM
TRAHMADPHIVQKIREGREDDIRPCVGATFCLDRIYQGGEALCIHNAATGRELSMPHVIAPAPAPRHVVIVGAGPAGLEA
ARVAAERGHVVTVFEAQADPGGQIRLTVQNPRRRDMISIIDWRMAQCAARDVRFHFNTWAEPETITDLNPDVVIVATGGL
PNTELFEAGRDTPHVISSWEMIAGDVKPADHVLIYDESGDHPGLQAAETAALAGAKVEVMTPDRVFAPDIMAMNLTPYMR
ALQDKDVTFTVTRRLLDVARNGNKLRATIGTDYSDHSYQTDYDQIVVNYGTFPLDELYHALKPLSSNLGAVDHDALIAGE
VQNVNTNSDGAFQLFRIGDAVSARNTHAAIYDALRLVKDL

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=530, Percent_Identity=29.622641509434, Blast_Score=200, Evalue=3e-52,
Organism=Escherichia coli, GI1787939, Length=390, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17559802, Length=257, Percent_Identity=28.7937743190661, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=260, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=260, Percent_Identity=28.0769230769231, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17565138, Length=333, Percent_Identity=24.6246246246246, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17564188, Length=261, Percent_Identity=25.6704980842912, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=208, Percent_Identity=29.3269230769231, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74809; Mature: 74678

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDPLMQPFQLKHLTLKNRIMTTSHEPAYPEDGMPKERYRAYHEERAKAGLALAMTAGS
CCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCC
AAVSKDSPPVFNNVLAYKDEVVPWITDLTDACHAHGCAVMIQLTHLGRRTTWNKGDWLPS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
LSSSRHREPAHRAFPKLIEDWDIDRVITDFADAAERMQAGGMDGIELQVYGHLLDQFWSP
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
LTNDLSGPYGADTLENRMRFPLDVLGAIRKRVGEDFIIGFRFTADEVQKGGITPEEGLRI
HHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEHHHHHHCCCCHHHCCEE
SKILADDGRLDFLNVIRGRIHTDPAMVDVIPVQGMSNAPHLDFAGAVKKATGLPTFHAAK
EEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCC
IPDVATARHAVANGLLDMVGMTRAHMADPHIVQKIREGREDDIRPCVGATFCLDRIYQGG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
EALCIHNAATGRELSMPHVIAPAPAPRHVVIVGAGPAGLEAARVAAERGHVVTVFEAQAD
CEEEEECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCC
PGGQIRLTVQNPRRRDMISIIDWRMAQCAARDVRFHFNTWAEPETITDLNPDVVIVATGG
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCC
LPNTELFEAGRDTPHVISSWEMIAGDVKPADHVLIYDESGDHPGLQAAETAALAGAKVEV
CCCHHHHHCCCCCCHHHHCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEE
MTPDRVFAPDIMAMNLTPYMRALQDKDVTFTVTRRLLDVARNGNKLRATIGTDYSDHSYQ
ECCCCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
TDYDQIVVNYGTFPLDELYHALKPLSSNLGAVDHDALIAGEVQNVNTNSDGAFQLFRIGD
CCHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECC
AVSARNTHAAIYDALRLVKDL
CCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKDPLMQPFQLKHLTLKNRIMTTSHEPAYPEDGMPKERYRAYHEERAKAGLALAMTAGS
CCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCC
AAVSKDSPPVFNNVLAYKDEVVPWITDLTDACHAHGCAVMIQLTHLGRRTTWNKGDWLPS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
LSSSRHREPAHRAFPKLIEDWDIDRVITDFADAAERMQAGGMDGIELQVYGHLLDQFWSP
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
LTNDLSGPYGADTLENRMRFPLDVLGAIRKRVGEDFIIGFRFTADEVQKGGITPEEGLRI
HHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEHHHHHHCCCCHHHCCEE
SKILADDGRLDFLNVIRGRIHTDPAMVDVIPVQGMSNAPHLDFAGAVKKATGLPTFHAAK
EEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCC
IPDVATARHAVANGLLDMVGMTRAHMADPHIVQKIREGREDDIRPCVGATFCLDRIYQGG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
EALCIHNAATGRELSMPHVIAPAPAPRHVVIVGAGPAGLEAARVAAERGHVVTVFEAQAD
CEEEEECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCC
PGGQIRLTVQNPRRRDMISIIDWRMAQCAARDVRFHFNTWAEPETITDLNPDVVIVATGG
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCC
LPNTELFEAGRDTPHVISSWEMIAGDVKPADHVLIYDESGDHPGLQAAETAALAGAKVEV
CCCHHHHHCCCCCCHHHHCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEE
MTPDRVFAPDIMAMNLTPYMRALQDKDVTFTVTRRLLDVARNGNKLRATIGTDYSDHSYQ
ECCCCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
TDYDQIVVNYGTFPLDELYHALKPLSSNLGAVDHDALIAGEVQNVNTNSDGAFQLFRIGD
CCHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECC
AVSARNTHAAIYDALRLVKDL
CCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]