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The map label for this gene is argF

Identifier: 110678980

GI number: 110678980

Start: 1612796

End: 1613722

Strand: Direct

Name: argF

Synonym: RD1_1677

Alternate gene names: 110678980

Gene position: 1612796-1613722 (Clockwise)

Preceding gene: 110678979

Following gene: 110678982

Centisome position: 39.02

GC content: 61.38

Gene sequence:

>927_bases
ATGAACCACTTTCTCGACATCCACCTGACGGACCCTGCGGATTTGCGCGAAATCATTGATCAGGCCGCCATCATGAAAAG
CGCGCGCAAAGGTCGCCCGCGTGGGGCCCCGGATGACACGCAACCGCTGAAAGATCGCATCGTTGCGCTGATTTTCGAAA
AACCGTCCACCCGAACCCGCGTGTCTTTTGACGTCGGCGTGCGGCAGATGGGGGGGCAGACGATGGTCCTCTCTGGCGCC
GACATGCAGTTGGGTCACGGCGAAACAATCGCCGACACGGCCCGCGTGCTGAGCCGCTATGTCGACCTGATCATGATCCG
CACCTTTGATGAGGCTGTGCTGACCGAGATGGCCGAGTTTGCGACTGTCCCGGTGATCAACGGTCTGACGGATCGCACGC
ACCCCTGCCAGATCATGGCCGATATCCTCACCTTTGAAGAGCACCGCGGCCCGATTGCGGGCAAGAAGGTGGTCTGGTCC
GGGGATGGCAACAATGTCTGCGCGTCGTTTCTGCATGCCGCCGGGCAATTCGGCTTTGATCTGACCTTTACCGGTCCGCA
AACGCTCGACCCGGATGCGGAGTTCGTCGAACTCGCCCGGTCAAAAGGGGTGAATGTCGCGGTGGAACGTGACCCGGCCA
AGGCGGTCGAGGGCGCCGATCTCGTGGTCACGGATACGTGGGTTTCCATGCATGACGCGCAATCGGCGCGTGAACGCCGC
CACAATCAGCTCAGGCCCTACCGCGTGGATGCGGCGCTGATGTCGCGGGCCAAGCCGGAAGCCTTGTTCATGCATTGCCT
GCCGGCCCATCGCGACGATGAGGCCACATCCGAGGTGATGGACGGGCCGCAGTCGGTGATCTTTGACGAAGCGGAAAACA
GGTTGCACGCGCAAAAAGCCATCATGCGGTGGTGTCTGGCTGTCTAG

Upstream 100 bases:

>100_bases
CGCGTCTTGATGCCGCCGCGCGTCGTGTCTCAACGGCCTGAACCTCGAACCTTTAGTATGCAGGGCGCGACAGTCGCCCG
CCTTGAGCAGAGAAAATATA

Downstream 100 bases:

>100_bases
AGCGTCTGACGAAATACCTGAAACATCAAGTATCACGTAACGCGTTGAAATCTTTGATTTCAGGCAGCGTTGCGTGCTTC
AGATTTTTCGCATGACGCTT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNHFLDIHLTDPADLREIIDQAAIMKSARKGRPRGAPDDTQPLKDRIVALIFEKPSTRTRVSFDVGVRQMGGQTMVLSGA
DMQLGHGETIADTARVLSRYVDLIMIRTFDEAVLTEMAEFATVPVINGLTDRTHPCQIMADILTFEEHRGPIAGKKVVWS
GDGNNVCASFLHAAGQFGFDLTFTGPQTLDPDAEFVELARSKGVNVAVERDPAKAVEGADLVVTDTWVSMHDAQSARERR
HNQLRPYRVDAALMSRAKPEALFMHCLPAHRDDEATSEVMDGPQSVIFDEAENRLHAQKAIMRWCLAV

Sequences:

>Translated_308_residues
MNHFLDIHLTDPADLREIIDQAAIMKSARKGRPRGAPDDTQPLKDRIVALIFEKPSTRTRVSFDVGVRQMGGQTMVLSGA
DMQLGHGETIADTARVLSRYVDLIMIRTFDEAVLTEMAEFATVPVINGLTDRTHPCQIMADILTFEEHRGPIAGKKVVWS
GDGNNVCASFLHAAGQFGFDLTFTGPQTLDPDAEFVELARSKGVNVAVERDPAKAVEGADLVVTDTWVSMHDAQSARERR
HNQLRPYRVDAALMSRAKPEALFMHCLPAHRDDEATSEVMDGPQSVIFDEAENRLHAQKAIMRWCLAV
>Mature_308_residues
MNHFLDIHLTDPADLREIIDQAAIMKSARKGRPRGAPDDTQPLKDRIVALIFEKPSTRTRVSFDVGVRQMGGQTMVLSGA
DMQLGHGETIADTARVLSRYVDLIMIRTFDEAVLTEMAEFATVPVINGLTDRTHPCQIMADILTFEEHRGPIAGKKVVWS
GDGNNVCASFLHAAGQFGFDLTFTGPQTLDPDAEFVELARSKGVNVAVERDPAKAVEGADLVVTDTWVSMHDAQSARERR
HNQLRPYRVDAALMSRAKPEALFMHCLPAHRDDEATSEVMDGPQSVIFDEAENRLHAQKAIMRWCLAV

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=264, Percent_Identity=37.1212121212121, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI18105007, Length=264, Percent_Identity=26.8939393939394, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=39.5136778115502, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=37.9939209726444, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI2367364, Length=316, Percent_Identity=27.5316455696203, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994908, Length=345, Percent_Identity=24.9275362318841, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=301, Percent_Identity=24.5847176079734, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=311, Percent_Identity=32.1543408360129, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=279, Percent_Identity=26.5232974910394, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=25.4838709677419, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_ROSDO (Q169P2)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681987.1
- ProteinModelPortal:   Q169P2
- SMR:   Q169P2
- STRING:   Q169P2
- GeneID:   4197051
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1677
- NMPDR:   fig|375451.6.peg.1571
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   Q169P2
- ProtClustDB:   PRK00779
- BioCyc:   RDEN375451:RD1_1677-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34101; Mature: 34101

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 107-107 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHFLDIHLTDPADLREIIDQAAIMKSARKGRPRGAPDDTQPLKDRIVALIFEKPSTRTR
CCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCCCCEE
VSFDVGVRQMGGQTMVLSGADMQLGHGETIADTARVLSRYVDLIMIRTFDEAVLTEMAEF
EEEEHHHHHCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVPVINGLTDRTHPCQIMADILTFEEHRGPIAGKKVVWSGDGNNVCASFLHAAGQFGFD
HHCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCE
LTFTGPQTLDPDAEFVELARSKGVNVAVERDPAKAVEGADLVVTDTWVSMHDAQSARERR
EEECCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHCCCCEEEEECHHHHHHHHHHHHHH
HNQLRPYRVDAALMSRAKPEALFMHCLPAHRDDEATSEVMDGPQSVIFDEAENRLHAQKA
HHCCCCEEECHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
IMRWCLAV
HHHHHHCC
>Mature Secondary Structure
MNHFLDIHLTDPADLREIIDQAAIMKSARKGRPRGAPDDTQPLKDRIVALIFEKPSTRTR
CCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCCCCEE
VSFDVGVRQMGGQTMVLSGADMQLGHGETIADTARVLSRYVDLIMIRTFDEAVLTEMAEF
EEEEHHHHHCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVPVINGLTDRTHPCQIMADILTFEEHRGPIAGKKVVWSGDGNNVCASFLHAAGQFGFD
HHCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCE
LTFTGPQTLDPDAEFVELARSKGVNVAVERDPAKAVEGADLVVTDTWVSMHDAQSARERR
EEECCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHCCCCEEEEECHHHHHHHHHHHHHH
HNQLRPYRVDAALMSRAKPEALFMHCLPAHRDDEATSEVMDGPQSVIFDEAENRLHAQKA
HHCCCCEEECHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
IMRWCLAV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA