Click here to switch to the map view.

The map label for this gene is plsB [H]

Identifier: 110678942

GI number: 110678942

Start: 1575269

End: 1576639

Strand: Direct

Name: plsB [H]

Synonym: RD1_1636

Alternate gene names: 110678942

Gene position: 1575269-1576639 (Clockwise)

Preceding gene: 110678939

Following gene: 110678943

Centisome position: 38.11

GC content: 60.47

Gene sequence:

>1371_bases
ATGACTTCGACAGTGGAATTGCCGGTTTGGTTCTTTGTTCTGATCCTGCTGTTTGCAGCAGTGACCTTTGCGTCCCACTT
TCTATTTCCTTCCGTACGGTGGTTTTTCCGGCGGCGTCTTGAACGGGCCGTTTCACAACTGAACGAACGCCTGACGCGTC
CGATTGAACCTTTCAAACTGGCGCGGCGCTACGACATGATCCAGCGCCTGATTTACGACCCCGAAGTCACGCGCGAAATC
GTCAACTACGCAAAAAGCCATGGTGTGCCAGAGAACGTAGGCTTTGAAAAAGCCCGCAGCTATGCCCGTGAAATCGTGCC
CAGTTTCAGTGCCGTGACCTATTTCGGGCTTGGGACGCGTCTGGCGCGCATGCTGGTCAACTCGATCTACGAGGTATGGA
CCGGCCCGCAGAACGACGAAATCATCCGGTCCATTTCCAAGGACGCCACGATCGTTTTCATCATGAACCACCGTTCCAAC
ATGGACTACGTGCTGGTGACCTATCTGGCCGCGCAGACATCGGCCCTGTCCTACGCGGTGGGGGAATGGGCGCGTGTCTG
GCCGCTCAGCCGGTTGATCCGGATGATGGGAGCCTATTTCATCCGGCGTCGCTCGCGCGGGGCGCTCTATCGCAAGGTGT
TGGAGCGGTATGTGCAGATGGCGACTGACGGCGGCGTGGCACAGGGAATCTTCCCCGAAGGCGGGCTCAGTCTGACGGGA
CGCTTGATGCCGCCCAAGATGGGGCTGTTGTCCTATCTGGCGCAGGGGCCGAACGGGCCGGCGCGCGATGTGGTCTTTGT
GCCGGTTGCGATTAACTATGACCGCGTTCTAGAGGACCGGGTGCTGATCGCGGCCGCTCAACGAGGGGATCGTCGTTTTG
GCGCGCGGATATCGGTCGCCGTGCGGTTCATATTGCGCAAGTTCTGGCAGGCACTGCGCGGCCGCTACGTGCGGTTCGGC
ACCGCAGCGGTGGTTTTCGGCGAGCCGTTGTCCATTCGGGGCTTTTGCGCTGAGCGGTCGGTGGAGGATCTGGCCGCCGA
ACTGATGACACGGATCGAAGACGCCATGCCGGTCCTGATCGTTCCCTTGCTGGCGCGTCAATTCCAGAAACGGCCCGGAC
CGGTGTCACAGGACGAACTGGTTAATGAGGTGGCGCGCGATGCGGCCAATCTGGGCGATCACATGCCGCGGATGTCGCCG
CCGGATCTGGCCAAGGCCGTGGGCAAGGGGCTGGTGCGCATGCGGCGCAACAAAGTGCTGGAACAGCGCGGCGCGCAGTG
GCAGACGATTGGCTCCGAGCGTGCGGTCATTGATTTCTACGCAAATTCGATTGCCCACAGATTTCCGGAGGATCAGGTCG
CCGCATCATGA

Upstream 100 bases:

>100_bases
GCCGTCGGGGTGATGCAGGAAAACGATGCTCATGCGCAGAACACCCGCTTGCGCGCACGAAAAAGGTTTACCCGTCGGCC
AATTCTTGAAACAAGCCAGC

Downstream 100 bases:

>100_bases
TTTCTGCAGGTGCTCGGTCATAAAGTTACAAAAATGAGGTCTCATATGTTGCAATGTTGCGTATGAGAACTTATTCAGAG
CGCAGAGCGACGCGATTCTG

Product: acyltransferase, putative

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTSTVELPVWFFVLILLFAAVTFASHFLFPSVRWFFRRRLERAVSQLNERLTRPIEPFKLARRYDMIQRLIYDPEVTREI
VNYAKSHGVPENVGFEKARSYAREIVPSFSAVTYFGLGTRLARMLVNSIYEVWTGPQNDEIIRSISKDATIVFIMNHRSN
MDYVLVTYLAAQTSALSYAVGEWARVWPLSRLIRMMGAYFIRRRSRGALYRKVLERYVQMATDGGVAQGIFPEGGLSLTG
RLMPPKMGLLSYLAQGPNGPARDVVFVPVAINYDRVLEDRVLIAAAQRGDRRFGARISVAVRFILRKFWQALRGRYVRFG
TAAVVFGEPLSIRGFCAERSVEDLAAELMTRIEDAMPVLIVPLLARQFQKRPGPVSQDELVNEVARDAANLGDHMPRMSP
PDLAKAVGKGLVRMRRNKVLEQRGAQWQTIGSERAVIDFYANSIAHRFPEDQVAAS

Sequences:

>Translated_456_residues
MTSTVELPVWFFVLILLFAAVTFASHFLFPSVRWFFRRRLERAVSQLNERLTRPIEPFKLARRYDMIQRLIYDPEVTREI
VNYAKSHGVPENVGFEKARSYAREIVPSFSAVTYFGLGTRLARMLVNSIYEVWTGPQNDEIIRSISKDATIVFIMNHRSN
MDYVLVTYLAAQTSALSYAVGEWARVWPLSRLIRMMGAYFIRRRSRGALYRKVLERYVQMATDGGVAQGIFPEGGLSLTG
RLMPPKMGLLSYLAQGPNGPARDVVFVPVAINYDRVLEDRVLIAAAQRGDRRFGARISVAVRFILRKFWQALRGRYVRFG
TAAVVFGEPLSIRGFCAERSVEDLAAELMTRIEDAMPVLIVPLLARQFQKRPGPVSQDELVNEVARDAANLGDHMPRMSP
PDLAKAVGKGLVRMRRNKVLEQRGAQWQTIGSERAVIDFYANSIAHRFPEDQVAAS
>Mature_455_residues
TSTVELPVWFFVLILLFAAVTFASHFLFPSVRWFFRRRLERAVSQLNERLTRPIEPFKLARRYDMIQRLIYDPEVTREIV
NYAKSHGVPENVGFEKARSYAREIVPSFSAVTYFGLGTRLARMLVNSIYEVWTGPQNDEIIRSISKDATIVFIMNHRSNM
DYVLVTYLAAQTSALSYAVGEWARVWPLSRLIRMMGAYFIRRRSRGALYRKVLERYVQMATDGGVAQGIFPEGGLSLTGR
LMPPKMGLLSYLAQGPNGPARDVVFVPVAINYDRVLEDRVLIAAAQRGDRRFGARISVAVRFILRKFWQALRGRYVRFGT
AAVVFGEPLSIRGFCAERSVEDLAAELMTRIEDAMPVLIVPLLARQFQKRPGPVSQDELVNEVARDAANLGDHMPRMSPP
DLAKAVGKGLVRMRRNKVLEQRGAQWQTIGSERAVIDFYANSIAHRFPEDQVAAS

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI190358539, Length=268, Percent_Identity=26.4925373134328, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87082362, Length=322, Percent_Identity=27.0186335403727, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988723, Length=138, Percent_Identity=31.8840579710145, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988728, Length=138, Percent_Identity=31.8840579710145, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25147672, Length=201, Percent_Identity=26.3681592039801, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650754, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650752, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357731, Length=192, Percent_Identity=30.7291666666667, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17864692, Length=278, Percent_Identity=25.8992805755396, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 51772; Mature: 51641

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTVELPVWFFVLILLFAAVTFASHFLFPSVRWFFRRRLERAVSQLNERLTRPIEPFKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ARRYDMIQRLIYDPEVTREIVNYAKSHGVPENVGFEKARSYAREIVPSFSAVTYFGLGTR
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LARMLVNSIYEVWTGPQNDEIIRSISKDATIVFIMNHRSNMDYVLVTYLAAQTSALSYAV
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GEWARVWPLSRLIRMMGAYFIRRRSRGALYRKVLERYVQMATDGGVAQGIFPEGGLSLTG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RLMPPKMGLLSYLAQGPNGPARDVVFVPVAINYDRVLEDRVLIAAAQRGDRRFGARISVA
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VRFILRKFWQALRGRYVRFGTAAVVFGEPLSIRGFCAERSVEDLAAELMTRIEDAMPVLI
HHHHHHHHHHHHCCCEEEECCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
VPLLARQFQKRPGPVSQDELVNEVARDAANLGDHMPRMSPPDLAKAVGKGLVRMRRNKVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EQRGAQWQTIGSERAVIDFYANSIAHRFPEDQVAAS
HHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TSTVELPVWFFVLILLFAAVTFASHFLFPSVRWFFRRRLERAVSQLNERLTRPIEPFKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ARRYDMIQRLIYDPEVTREIVNYAKSHGVPENVGFEKARSYAREIVPSFSAVTYFGLGTR
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LARMLVNSIYEVWTGPQNDEIIRSISKDATIVFIMNHRSNMDYVLVTYLAAQTSALSYAV
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GEWARVWPLSRLIRMMGAYFIRRRSRGALYRKVLERYVQMATDGGVAQGIFPEGGLSLTG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RLMPPKMGLLSYLAQGPNGPARDVVFVPVAINYDRVLEDRVLIAAAQRGDRRFGARISVA
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VRFILRKFWQALRGRYVRFGTAAVVFGEPLSIRGFCAERSVEDLAAELMTRIEDAMPVLI
HHHHHHHHHHHHCCCEEEECCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
VPLLARQFQKRPGPVSQDELVNEVARDAANLGDHMPRMSPPDLAKAVGKGLVRMRRNKVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EQRGAQWQTIGSERAVIDFYANSIAHRFPEDQVAAS
HHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA