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The map label for this gene is nirE [H]

Identifier: 110678873

GI number: 110678873

Start: 1489854

End: 1490645

Strand: Direct

Name: nirE [H]

Synonym: RD1_1566

Alternate gene names: 110678873

Gene position: 1489854-1490645 (Clockwise)

Preceding gene: 110678872

Following gene: 110678874

Centisome position: 36.05

GC content: 62.88

Gene sequence:

>792_bases
ATGAACCAAGGTCAGGTCTTTTTGGTCGGTGCAGGGCCGGGCGATCCCGAATTGCTGACGCTGCGCGCGCTGCGGATGAT
CCAATCAGCGGATGTGGTGGTCCATGATCGGCTGGTATCACGCGATATCATGGCGCTCGTGCCTGCGGGTGTGCGTCGGA
TCAATGTGGGCAAGAAACCCAACCACCATCCGGTGCCGCAGGATGAAATCAATGCCCTGTTGGTGCAATTGGTTCAGGAG
GGGGCGACCGTCGCCCGCCTCAAAGGCGGCGACCCGATGATTTTCGGACGCGGTTCCGAAGAGGCCGCCGTTCTGCACGC
CGCCGGTATCAAGGTGAGTTACGCTCCCGGTATCACAGCCGCGCAGGGCGTGTCTGCATCAACGGGCGTGCCGCTGACGC
ACCGCGGATTGGCCACGGGCGTGCGCTATGTCACCGGTCATTGTCAGGCCAACCGTCCGCTTGACCTCGACTGGGACGGG
TTGGCGGATGCGGATACCACGCTGGTCGTCTATATGGGCCACGCCAATATGCAACAGATCGCGGCTGGCCTGATCGAACG
CGGGCTGCCTGCGGAACATCCGGTTCTGGTGGTCGCAAATGGCACGCGGCCCAATGAAACCCGTCTCGTGACGCAATTGG
GAGCCTTGCAGATCAAACGTGCCAATATTCCCGTGCCCGGTCCGGTTCTGTTCATCGTGGGCCGCGTCGTCACCCTGATC
GACAGCGAGGACAGCACGGCGGCGGACACGCTTCTCCGGGCCCCCGAACTGCAGCGGGTGCTGCATGGCTAG

Upstream 100 bases:

>100_bases
CAAACCGGGCGCGGCAGGACTCTCCCCCCTGCCGCGCTTTTTCCAACACTGTGGCCCCAAAATGCAACTGAACTGGTAAG
GACTTGAAATGCTGAAGAAA

Downstream 100 bases:

>100_bases
GGCGGTCGCATTGCTGGCGATATCTGTGCTTGCCGCGCCCTCTCTCGCTGCCGAAGCACTGCCGCAAGCCGAACAGGACC
GCCTTGCGCATTTCGTGCAA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNQGQVFLVGAGPGDPELLTLRALRMIQSADVVVHDRLVSRDIMALVPAGVRRINVGKKPNHHPVPQDEINALLVQLVQE
GATVARLKGGDPMIFGRGSEEAAVLHAAGIKVSYAPGITAAQGVSASTGVPLTHRGLATGVRYVTGHCQANRPLDLDWDG
LADADTTLVVYMGHANMQQIAAGLIERGLPAEHPVLVVANGTRPNETRLVTQLGALQIKRANIPVPGPVLFIVGRVVTLI
DSEDSTAADTLLRAPELQRVLHG

Sequences:

>Translated_263_residues
MNQGQVFLVGAGPGDPELLTLRALRMIQSADVVVHDRLVSRDIMALVPAGVRRINVGKKPNHHPVPQDEINALLVQLVQE
GATVARLKGGDPMIFGRGSEEAAVLHAAGIKVSYAPGITAAQGVSASTGVPLTHRGLATGVRYVTGHCQANRPLDLDWDG
LADADTTLVVYMGHANMQQIAAGLIERGLPAEHPVLVVANGTRPNETRLVTQLGALQIKRANIPVPGPVLFIVGRVVTLI
DSEDSTAADTLLRAPELQRVLHG
>Mature_263_residues
MNQGQVFLVGAGPGDPELLTLRALRMIQSADVVVHDRLVSRDIMALVPAGVRRINVGKKPNHHPVPQDEINALLVQLVQE
GATVARLKGGDPMIFGRGSEEAAVLHAAGIKVSYAPGITAAQGVSASTGVPLTHRGLATGVRYVTGHCQANRPLDLDWDG
LADADTTLVVYMGHANMQQIAAGLIERGLPAEHPVLVVANGTRPNETRLVTQLGALQIKRANIPVPGPVLFIVGRVVTLI
DSEDSTAADTLLRAPELQRVLHG

Specific function: Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin. Inactivation of uroporphyrinogen-III methyltran

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=51.2295081967213, Blast_Score=219, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322922, Length=246, Percent_Identity=29.2682926829268, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27784; Mature: 27784

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQGQVFLVGAGPGDPELLTLRALRMIQSADVVVHDRLVSRDIMALVPAGVRRINVGKKP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCCEEEECCCCC
NHHPVPQDEINALLVQLVQEGATVARLKGGDPMIFGRGSEEAAVLHAAGIKVSYAPGITA
CCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCEEEEEECCEEEEECCCCCH
AQGVSASTGVPLTHRGLATGVRYVTGHCQANRPLDLDWDGLADADTTLVVYMGHANMQQI
HCCCCCCCCCCEEHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
AAGLIERGLPAEHPVLVVANGTRPNETRLVTQLGALQIKRANIPVPGPVLFIVGRVVTLI
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHEEE
DSEDSTAADTLLRAPELQRVLHG
CCCCCHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MNQGQVFLVGAGPGDPELLTLRALRMIQSADVVVHDRLVSRDIMALVPAGVRRINVGKKP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCCEEEECCCCC
NHHPVPQDEINALLVQLVQEGATVARLKGGDPMIFGRGSEEAAVLHAAGIKVSYAPGITA
CCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCEEEEEECCEEEEECCCCCH
AQGVSASTGVPLTHRGLATGVRYVTGHCQANRPLDLDWDGLADADTTLVVYMGHANMQQI
HCCCCCCCCCCEEHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
AAGLIERGLPAEHPVLVVANGTRPNETRLVTQLGALQIKRANIPVPGPVLFIVGRVVTLI
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHEEE
DSEDSTAADTLLRAPELQRVLHG
CCCCCHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7747927 [H]