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The map label for this gene is dacB [C]

Identifier: 110678570

GI number: 110678570

Start: 1206605

End: 1208104

Strand: Direct

Name: dacB [C]

Synonym: RD1_1244

Alternate gene names: 110678570

Gene position: 1206605-1208104 (Clockwise)

Preceding gene: 110678569

Following gene: 110678572

Centisome position: 29.19

GC content: 65.87

Gene sequence:

>1500_bases
ATGACCAAGACACCTTGTTCGAGTTTTTCACGCCGCGCTGTGGTGCAGGCGTTGATGGCGACGCTTGCGACACCTCTGGC
CGCCGGCGCGCCGCTTGTGTCCGAGCGTCCCCCAAGAGGGCGTGGCCCCGTGCGCCCGCCCGGTCCGGAGGCGCTGGTGA
AAAAGGCGCGGCTGTCGGGTGATGTAGCCTTTGCGGTTGCGGATGCCAAAACCGGTGAGATCCTTGAGGCGCGCGCCCCC
GAACTGGGTATCGCGCCCGCCAGCGTGACCAAGGCGATCACGGCGCTTTATGCGCTGGACACATTGGGGGCCGCGCACCG
GTTCAAGACCCGCGTGATTGCGACGGGCGGTGTGACGGATGGTGTGGTCCGGGGCGATCTGGTTCTGGCCGGTGGGTGTG
ATCCGACAACGGATACGCGCGCCTTTGCAGCGCTGGCCGCACAGTTGAAAGAGGCGGGCATTCGCGAGGTCAAAGGGGAT
TTCCGTGTCTACGAAGGGCCCGTTGCCACGGTGCGTTCCATCGATCCTGAGCAGCCGGATCATGTGGGCTACAGCCCGGC
GGTGGCCGGGATCGCGCTCAACTTCAACCGTGTCCATTTCGAATGGAAACGGGCGGGGGGCAAATACACCGTGACCATGG
ATGCGCGCGCAGGGCGCTACCGCCCGGAGGTGGCGGTTGCGCGTATGAAGGTCGTGCAACGGCGCGCGCCGATTTACACC
TATGAGGAAGCGAACGGCCGCGATAACTGGACCGTCGCCAGTGGCGCCTTGGGGTCGGGCGGGTCGCGCTGGCTGCCGGT
GCGCCGACCGGGGCTTTATGCGGGGGAGGTGTTTGCAACGCTCGCCCGGTCGCACGGGATCGTTCTGCCTGCGGCCAAGC
TGCAAAAGGCAGCGCCACAAGGGGCCGTTCTGGCCGAGATCGAGAGCGCGCCACTCAGTGACGTGTTGCGCGCGATGCTG
AAGTATTCAAACAACCTGACCGCTGAAATGATCGGAATGGCGGCGACGCAGGCGCGGACCGGGCAGGTGCGCAGCCTTGC
CGCGTCAGCGCGCGAGATGAATGCATGGGCCAAGGATGTGCTGAAAATGCAAAGCCCGGCCTTTGTCGATCACTCAGGAC
TTGGGGGCGACAGCCGGGTCAGCGCGCAGGATATGGTGCGCGGCCTCGGGGCCATTGGTCACGCGGCCTTGCTGCGCCCG
CTGCTCAAGTCGGTGAAGCTATTGGACTCGCAGGGGCGTCTGGTCAATGAGCACCCGGTGCAGGCGGATGCCAAAACCGG
CACGCTGAATTTCGTTTCGGGACTGGGCGGCTATGTCACAACGCCCGAGGGGCGCGAGTTGGTATTCGCCATTTTTGCCG
CCGATGAAGCCACAAGGGCGGGCATTACCCGTGCGCAGCGCGAACGCCCACCCGGCGGGCGCAGCTATGCCACACGGGCG
CGCCGCCTGCAGCGGCAGTTGATCATGCGGTGGGGGGCGGTCTACGACCCGGCGGCGTAA

Upstream 100 bases:

>100_bases
AAGGGTGGCTGGGTCTGAGGCTGCCTGCGTCCGCAATCGGTTCAAAATGATGGCCTTTGCACTGTTGTTTCTGCGCCCCG
GCTGGGGGTAGGGTTTGACC

Downstream 100 bases:

>100_bases
TCCGATGTTGGTCGCAGGGCGGGCCTGCGTCCTCGGTCGGGCCGCGGTCAGGTCAGGTGGCGCGCCCGGGCCCCGCGTTC
GATGGCGGCAGCATGCAGGC

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: D-Alanyl-D-Alanine Carboxypeptidase/Endopeptidase; D-Alanyl-D-Alanine Carboxypeptidase

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTKTPCSSFSRRAVVQALMATLATPLAAGAPLVSERPPRGRGPVRPPGPEALVKKARLSGDVAFAVADAKTGEILEARAP
ELGIAPASVTKAITALYALDTLGAAHRFKTRVIATGGVTDGVVRGDLVLAGGCDPTTDTRAFAALAAQLKEAGIREVKGD
FRVYEGPVATVRSIDPEQPDHVGYSPAVAGIALNFNRVHFEWKRAGGKYTVTMDARAGRYRPEVAVARMKVVQRRAPIYT
YEEANGRDNWTVASGALGSGGSRWLPVRRPGLYAGEVFATLARSHGIVLPAAKLQKAAPQGAVLAEIESAPLSDVLRAML
KYSNNLTAEMIGMAATQARTGQVRSLAASAREMNAWAKDVLKMQSPAFVDHSGLGGDSRVSAQDMVRGLGAIGHAALLRP
LLKSVKLLDSQGRLVNEHPVQADAKTGTLNFVSGLGGYVTTPEGRELVFAIFAADEATRAGITRAQRERPPGGRSYATRA
RRLQRQLIMRWGAVYDPAA

Sequences:

>Translated_499_residues
MTKTPCSSFSRRAVVQALMATLATPLAAGAPLVSERPPRGRGPVRPPGPEALVKKARLSGDVAFAVADAKTGEILEARAP
ELGIAPASVTKAITALYALDTLGAAHRFKTRVIATGGVTDGVVRGDLVLAGGCDPTTDTRAFAALAAQLKEAGIREVKGD
FRVYEGPVATVRSIDPEQPDHVGYSPAVAGIALNFNRVHFEWKRAGGKYTVTMDARAGRYRPEVAVARMKVVQRRAPIYT
YEEANGRDNWTVASGALGSGGSRWLPVRRPGLYAGEVFATLARSHGIVLPAAKLQKAAPQGAVLAEIESAPLSDVLRAML
KYSNNLTAEMIGMAATQARTGQVRSLAASAREMNAWAKDVLKMQSPAFVDHSGLGGDSRVSAQDMVRGLGAIGHAALLRP
LLKSVKLLDSQGRLVNEHPVQADAKTGTLNFVSGLGGYVTTPEGRELVFAIFAADEATRAGITRAQRERPPGGRSYATRA
RRLQRQLIMRWGAVYDPAA
>Mature_498_residues
TKTPCSSFSRRAVVQALMATLATPLAAGAPLVSERPPRGRGPVRPPGPEALVKKARLSGDVAFAVADAKTGEILEARAPE
LGIAPASVTKAITALYALDTLGAAHRFKTRVIATGGVTDGVVRGDLVLAGGCDPTTDTRAFAALAAQLKEAGIREVKGDF
RVYEGPVATVRSIDPEQPDHVGYSPAVAGIALNFNRVHFEWKRAGGKYTVTMDARAGRYRPEVAVARMKVVQRRAPIYTY
EEANGRDNWTVASGALGSGGSRWLPVRRPGLYAGEVFATLARSHGIVLPAAKLQKAAPQGAVLAEIESAPLSDVLRAMLK
YSNNLTAEMIGMAATQARTGQVRSLAASAREMNAWAKDVLKMQSPAFVDHSGLGGDSRVSAQDMVRGLGAIGHAALLRPL
LKSVKLLDSQGRLVNEHPVQADAKTGTLNFVSGLGGYVTTPEGRELVFAIFAADEATRAGITRAQRERPPGGRSYATRAR
RLQRQLIMRWGAVYDPAA

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 52897; Mature: 52766

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTPCSSFSRRAVVQALMATLATPLAAGAPLVSERPPRGRGPVRPPGPEALVKKARLSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
DVAFAVADAKTGEILEARAPELGIAPASVTKAITALYALDTLGAAHRFKTRVIATGGVTD
CEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
GVVRGDLVLAGGCDPTTDTRAFAALAAQLKEAGIREVKGDFRVYEGPVATVRSIDPEQPD
CEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCC
HVGYSPAVAGIALNFNRVHFEWKRAGGKYTVTMDARAGRYRPEVAVARMKVVQRRAPIYT
CCCCCHHHEEEEEECCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEE
YEEANGRDNWTVASGALGSGGSRWLPVRRPGLYAGEVFATLARSHGIVLPAAKLQKAAPQ
EECCCCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHCCCC
GAVLAEIESAPLSDVLRAMLKYSNNLTAEMIGMAATQARTGQVRSLAASAREMNAWAKDV
CCEEEHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKMQSPAFVDHSGLGGDSRVSAQDMVRGLGAIGHAALLRPLLKSVKLLDSQGRLVNEHPV
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QADAKTGTLNFVSGLGGYVTTPEGRELVFAIFAADEATRAGITRAQRERPPGGRSYATRA
CCCCCCCCCHHHHCCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHH
RRLQRQLIMRWGAVYDPAA
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKTPCSSFSRRAVVQALMATLATPLAAGAPLVSERPPRGRGPVRPPGPEALVKKARLSG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
DVAFAVADAKTGEILEARAPELGIAPASVTKAITALYALDTLGAAHRFKTRVIATGGVTD
CEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
GVVRGDLVLAGGCDPTTDTRAFAALAAQLKEAGIREVKGDFRVYEGPVATVRSIDPEQPD
CEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCC
HVGYSPAVAGIALNFNRVHFEWKRAGGKYTVTMDARAGRYRPEVAVARMKVVQRRAPIYT
CCCCCHHHEEEEEECCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEE
YEEANGRDNWTVASGALGSGGSRWLPVRRPGLYAGEVFATLARSHGIVLPAAKLQKAAPQ
EECCCCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHCCCC
GAVLAEIESAPLSDVLRAMLKYSNNLTAEMIGMAATQARTGQVRSLAASAREMNAWAKDV
CCEEEHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKMQSPAFVDHSGLGGDSRVSAQDMVRGLGAIGHAALLRPLLKSVKLLDSQGRLVNEHPV
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QADAKTGTLNFVSGLGGYVTTPEGRELVFAIFAADEATRAGITRAQRERPPGGRSYATRA
CCCCCCCCCHHHHCCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHH
RRLQRQLIMRWGAVYDPAA
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA