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The map label for this gene is yieH [H]

Identifier: 110678093

GI number: 110678093

Start: 700100

End: 700744

Strand: Direct

Name: yieH [H]

Synonym: RD1_0723

Alternate gene names: 110678093

Gene position: 700100-700744 (Clockwise)

Preceding gene: 110678091

Following gene: 110678094

Centisome position: 16.94

GC content: 61.4

Gene sequence:

>645_bases
ATGCCCCCCGAAATGGTTCTGTTTGATTGCGACGGCGTATTGGTCGATAGCGAAGCGCTGACGGCGGATGTGTTATGTGC
GAATCTGCGGCGGCATGGGCTGGCCCTGACCCATGCAGAGCTTGGCGCGATGTTTCTGGGCGGCACGATCATGGGGGTGG
AAGCAAAAGCGCTGGAACTGGGCGCCGACCTGCCCAGGGACTGGGTAGATCAGATTTACGCCGAGATTTTTGAAACGCTC
GGGACCTCCGTCCCGATCATTCCCGGTGTGCATGCGGTGCTTGATGCACTGGATGCTGCGGGCATTCTTTATGCGGTCGG
CTCGAATGGCCCGCATCGAAAGATGCAGATTACCCTGACAAAAACGGGCCTGATGGACCGGCTGGCCGGGCGCATCTATT
CACGCGAAGATGTTGCGCAGCCGAAACCCGCGCCCGATGTCTATCTGAAGGCGGCGGCAGATGCGGGGGTCGCGCCGGAG
CGCTGTGTCGTGATTGAAGACAGCCCCAATGGCGCGCGCGCCGGGCAGGCGGCGGGCATGTGGTGCCTTGGGTTTGCGGC
GGAAACCGATGCGGCCCTGTTGGCACCGGTGTGCGACGATGTCTTTTATGCAATGGGTGATCTGCCGGGGCTTTTGGGTC
TCTGA

Upstream 100 bases:

>100_bases
TCCAATAACGAATTTGTCAGAAGCTGTGAGTGATCTGCCGCTATTTCCCGCCTGAGCATTGACGACGGGCGGGCTGAGGT
GCACTACGAACCCCATGAAG

Downstream 100 bases:

>100_bases
CGGCGGTTTGTCCACTATTACAGCGTCAAACATTCTGGTGATCTGCGTGAAATCTCTCCGCAATTCATTTGGTCGCGCGT
GCTCGCGCGCCTACAACTGC

Product: hydrolase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPPEMVLFDCDGVLVDSEALTADVLCANLRRHGLALTHAELGAMFLGGTIMGVEAKALELGADLPRDWVDQIYAEIFETL
GTSVPIIPGVHAVLDALDAAGILYAVGSNGPHRKMQITLTKTGLMDRLAGRIYSREDVAQPKPAPDVYLKAAADAGVAPE
RCVVIEDSPNGARAGQAAGMWCLGFAAETDAALLAPVCDDVFYAMGDLPGLLGL

Sequences:

>Translated_214_residues
MPPEMVLFDCDGVLVDSEALTADVLCANLRRHGLALTHAELGAMFLGGTIMGVEAKALELGADLPRDWVDQIYAEIFETL
GTSVPIIPGVHAVLDALDAAGILYAVGSNGPHRKMQITLTKTGLMDRLAGRIYSREDVAQPKPAPDVYLKAAADAGVAPE
RCVVIEDSPNGARAGQAAGMWCLGFAAETDAALLAPVCDDVFYAMGDLPGLLGL
>Mature_213_residues
PPEMVLFDCDGVLVDSEALTADVLCANLRRHGLALTHAELGAMFLGGTIMGVEAKALELGADLPRDWVDQIYAEIFETLG
TSVPIIPGVHAVLDALDAAGILYAVGSNGPHRKMQITLTKTGLMDRLAGRIYSREDVAQPKPAPDVYLKAAADAGVAPER
CVVIEDSPNGARAGQAAGMWCLGFAAETDAALLAPVCDDVFYAMGDLPGLLGL

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=210, Percent_Identity=30, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87082080, Length=206, Percent_Identity=32.0388349514563, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788021, Length=177, Percent_Identity=30.5084745762712, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 22417; Mature: 22286

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPEMVLFDCDGVLVDSEALTADVLCANLRRHGLALTHAELGAMFLGGTIMGVEAKALEL
CCCCEEEEECCCEEECCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCEEECCCHHHHHH
GADLPRDWVDQIYAEIFETLGTSVPIIPGVHAVLDALDAAGILYAVGSNGPHRKMQITLT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEE
KTGLMDRLAGRIYSREDVAQPKPAPDVYLKAAADAGVAPERCVVIEDSPNGARAGQAAGM
CHHHHHHHHHHHCCCHHCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCE
WCLGFAAETDAALLAPVCDDVFYAMGDLPGLLGL
EEEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
PPEMVLFDCDGVLVDSEALTADVLCANLRRHGLALTHAELGAMFLGGTIMGVEAKALEL
CCCEEEEECCCEEECCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCEEECCCHHHHHH
GADLPRDWVDQIYAEIFETLGTSVPIIPGVHAVLDALDAAGILYAVGSNGPHRKMQITLT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEE
KTGLMDRLAGRIYSREDVAQPKPAPDVYLKAAADAGVAPERCVVIEDSPNGARAGQAAGM
CHHHHHHHHHHHCCCHHCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCE
WCLGFAAETDAALLAPVCDDVFYAMGDLPGLLGL
EEEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]