Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 110677636

GI number: 110677636

Start: 227780

End: 228886

Strand: Direct

Name: leuB [H]

Synonym: RD1_0228

Alternate gene names: 110677636

Gene position: 227780-228886 (Clockwise)

Preceding gene: 110677635

Following gene: 110677637

Centisome position: 5.51

GC content: 59.08

Gene sequence:

>1107_bases
ATGGCCAACCCTTCCCTTTTAATTCTTCCGGGTGACGGAATCGGCCCCGAAGTGATGGAGCAGGTCAAGCGCGTGATCGC
ATGGTTCGGCGACAAGCGCGACCTTGCGTTTGACGTCAGTGAAGACCTCGTAGGCGGTGCTGCCTATGACGCACATGGCA
CACCGCTGCATGACGAAACGATGGCGCGCGCGCTGCAGGTTGATGCGGTTTTGCTGGGTGCGGTTGGCGGACCTAAATAC
GACGATCTCGACTTCAGCGTGAAACCCGAACGCGGCTTGCTGCGCCTGCGCAAGGAAATGGACCTGTTTTCCAATCTGCG
GCCTGCACAGTGTTTTGATGCGCTGGCGGATTTTTCATCCCTCAAGAAAGACGTCGTTGCGGGACTCGACATCATGATCG
TGCGCGAATTGACGTCGGGTGTGTATTTCGGTGAGCCGCGCGGCATTTTTGAAGAGGGGAATGAGCGCGTCGGCGTCAAC
ACACAGCGCTATACGGAATCCGAGATTGACCGCGTCGCACGCTCCGCATTCGAACTGGCGCGCCGTCGGAACAACAAAGT
GTGCTCGATGGAAAAAGCCAATGTCATGGAAAGCGGCATCCTCTGGCGTGAGGTTGTGCAGAAAGTCCATGACGAGGATT
ACCCCGATGTTGAACTGAGCCATATGTACGCCGACAACGGCGCCATGCAGCTGGTGCGCGCGCCCAAGCAGTTCGATGTG
ATCCTCACGGACAACCTGTTTGGCGATATTCTGTCCGATTGCGCCGCGATGCTGACCGGCTCATTGGGCATGTTGCCATC
GGCGAGCCTTGGCGCGCCGGCGGCGAATGGTCGGCCCAAGGCGCTTTATGAGCCGGTGCATGGGTCAGCACCCGATATCA
CCGGGCAGGGAAAGGCCAACCCGATTGCCTGCATCCTGAGCTTTGCGATGGCGCTGCGCTATTCCTTTGATCAGGGCGAT
GAGGCCGCACGGCTTGAAGCTGCCATCGAGACGGTGCTTGCCAATGGCGCGCGCACCGCAGACCTGATGGGCGAAGAAGG
CGGCACACCAATCAGCACCACCGAAATGGGTGATGCGATCCTGAAGGCGCTGGACGCGAGCCTCTAA

Upstream 100 bases:

>100_bases
CCTTGTCTGGGTTGATCTGGCGCGTTGAAGCCTTTGGCCTTCTTGACCCTGCATCGCCCACACGGTAGGGCGCTACAGAC
CACTTTTCAGGAGAACCGCC

Downstream 100 bases:

>100_bases
GCACAGCAGCTATGCATCCAGCGCTCTTGCTGGATCAGGGTCATCGGCCTAAAGCGTCTGGGCAGCCCGCATTTTGCGGG
CCGCTTCCACGCGTATGAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MANPSLLILPGDGIGPEVMEQVKRVIAWFGDKRDLAFDVSEDLVGGAAYDAHGTPLHDETMARALQVDAVLLGAVGGPKY
DDLDFSVKPERGLLRLRKEMDLFSNLRPAQCFDALADFSSLKKDVVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGVN
TQRYTESEIDRVARSAFELARRRNNKVCSMEKANVMESGILWREVVQKVHDEDYPDVELSHMYADNGAMQLVRAPKQFDV
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPAANGRPKALYEPVHGSAPDITGQGKANPIACILSFAMALRYSFDQGD
EAARLEAAIETVLANGARTADLMGEEGGTPISTTEMGDAILKALDASL

Sequences:

>Translated_368_residues
MANPSLLILPGDGIGPEVMEQVKRVIAWFGDKRDLAFDVSEDLVGGAAYDAHGTPLHDETMARALQVDAVLLGAVGGPKY
DDLDFSVKPERGLLRLRKEMDLFSNLRPAQCFDALADFSSLKKDVVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGVN
TQRYTESEIDRVARSAFELARRRNNKVCSMEKANVMESGILWREVVQKVHDEDYPDVELSHMYADNGAMQLVRAPKQFDV
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPAANGRPKALYEPVHGSAPDITGQGKANPIACILSFAMALRYSFDQGD
EAARLEAAIETVLANGARTADLMGEEGGTPISTTEMGDAILKALDASL
>Mature_367_residues
ANPSLLILPGDGIGPEVMEQVKRVIAWFGDKRDLAFDVSEDLVGGAAYDAHGTPLHDETMARALQVDAVLLGAVGGPKYD
DLDFSVKPERGLLRLRKEMDLFSNLRPAQCFDALADFSSLKKDVVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGVNT
QRYTESEIDRVARSAFELARRRNNKVCSMEKANVMESGILWREVVQKVHDEDYPDVELSHMYADNGAMQLVRAPKQFDVI
LTDNLFGDILSDCAAMLTGSLGMLPSASLGAPAANGRPKALYEPVHGSAPDITGQGKANPIACILSFAMALRYSFDQGDE
AARLEAAIETVLANGARTADLMGEEGGTPISTTEMGDAILKALDASL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=351, Percent_Identity=33.9031339031339, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI28178816, Length=343, Percent_Identity=27.4052478134111, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI28178821, Length=356, Percent_Identity=26.9662921348315, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI4758582, Length=321, Percent_Identity=29.595015576324, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI28178838, Length=319, Percent_Identity=30.0940438871473, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI28178819, Length=195, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=52.4861878453039, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788101, Length=365, Percent_Identity=34.7945205479452, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787381, Length=419, Percent_Identity=25.0596658711217, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=371, Percent_Identity=33.1536388140162, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=374, Percent_Identity=27.0053475935829, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=314, Percent_Identity=29.2993630573248, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=27.9778393351801, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=42.5, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=370, Percent_Identity=34.0540540540541, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=32.1428571428571, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=278, Percent_Identity=32.7338129496403, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24643270, Length=349, Percent_Identity=33.810888252149, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643268, Length=349, Percent_Identity=33.810888252149, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078635, Length=370, Percent_Identity=29.1891891891892, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161078637, Length=370, Percent_Identity=29.1891891891892, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161078633, Length=370, Percent_Identity=29.1891891891892, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24650122, Length=370, Percent_Identity=29.1891891891892, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161078639, Length=367, Percent_Identity=29.4277929155313, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24661184, Length=369, Percent_Identity=29.5392953929539, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=27.0114942528736, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=27.0114942528736, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39757; Mature: 39625

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPSLLILPGDGIGPEVMEQVKRVIAWFGDKRDLAFDVSEDLVGGAAYDAHGTPLHDET
CCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCEECCCCCCCCHHH
MARALQVDAVLLGAVGGPKYDDLDFSVKPERGLLRLRKEMDLFSNLRPAQCFDALADFSS
HHHHHHHHHHEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKDVVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGVNTQRYTESEIDRVARSAFELA
HHHHHHHCCHHHHHHHHHCCEEECCCCCHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
RRRNNKVCSMEKANVMESGILWREVVQKVHDEDYPDVELSHMYADNGAMQLVRAPKQFDV
HHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCEEE
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPAANGRPKALYEPVHGSAPDITGQGKAN
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
PIACILSFAMALRYSFDQGDEAARLEAAIETVLANGARTADLMGEEGGTPISTTEMGDAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHH
LKALDASL
HHHHHCCC
>Mature Secondary Structure 
ANPSLLILPGDGIGPEVMEQVKRVIAWFGDKRDLAFDVSEDLVGGAAYDAHGTPLHDET
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCEECCCCCCCCHHH
MARALQVDAVLLGAVGGPKYDDLDFSVKPERGLLRLRKEMDLFSNLRPAQCFDALADFSS
HHHHHHHHHHEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKDVVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGVNTQRYTESEIDRVARSAFELA
HHHHHHHCCHHHHHHHHHCCEEECCCCCHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
RRRNNKVCSMEKANVMESGILWREVVQKVHDEDYPDVELSHMYADNGAMQLVRAPKQFDV
HHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCEEE
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPAANGRPKALYEPVHGSAPDITGQGKAN
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
PIACILSFAMALRYSFDQGDEAARLEAAIETVLANGARTADLMGEEGGTPISTTEMGDAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHH
LKALDASL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA