| Definition | Mesorhizobium sp. BNC1, complete genome. |
|---|---|
| Accession | NC_008254 |
| Length | 4,412,446 |
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The map label for this gene is lcfB [H]
Identifier: 110635215
GI number: 110635215
Start: 3135031
End: 3136575
Strand: Reverse
Name: lcfB [H]
Synonym: Meso_2882
Alternate gene names: 110635215
Gene position: 3136575-3135031 (Counterclockwise)
Preceding gene: 110635216
Following gene: 110635214
Centisome position: 71.08
GC content: 62.98
Gene sequence:
>1545_bases ATGCGTGTCGAAGAATTCCTGCGGGGCAGCGCCTCGCGCTACGGGGAAAAGACCGCTCTCGTGAGCGGGTCCACGCGGCT CAGCTATGGCGAGTTCGACGCGCTGACGGACCGCCTTGCAGCGGCGCTCGCGGCTAACGGCGTGCGGCGCAACGACCGCG TGCTCATCTTCATGGACAATGCGTGGGAGGCGGCGGTCTCCATCTTCGCTGTCCTCAAGGCCGGCGCCACCTTCAGCCCC ATCAACGCCTCCACAAAGGCGGAAAAGCTCGCCTACATCATCGGCAACTGCGAGGCTGCCGCAATCCTCACGCAGGCCAA GCTAATGCCGGTCGCGGCCGAGGCGCGCGCGCTTCACGGCCAGCCGCTTCTTCTGATCTCCACAGAAGGCCGGGGCGGTA CCGTACCGGACGGTACGGTATCTTTTACGCAGTGCCTTGAGGCCGAGCCGGCGCCGGTCGCCCATCGCGGCATCGATGTC GATCTCGCCATGCTCATCTACACATCGGGCTCCACCGGCCGGCCCAAGGGTGTGATGATGACGCATCGCAATATCGAAGC TGCGGCCACATCCATCACCACCTATCTGCGCAACAGGCCCGATGACATCATCCTCAACGTGTTGCCGCTCGCTTTCGACT ATGGGCTTTATCAGCTCCTGATGTCGGTCAAGATGGGCGCCACGCTGGTGCTGGAAAAATCCTTCGCCTTCCCGCATGCC ATCTTCGATCTGATGCGGAAGGAAAACGTAACCGGCTTTCCGCTTGTGCCGACCATGGCGGCGATGATCCTGCAGATGCG CGATCTGGAGCCCGGCTTCCTGCCCACGCTGCGCTACATCACCAACACCGCCGCCGCGCTTCCGCCGCCACATATCGCCA GGCTGCGCGAGCTGTGCCCTGGCATCGAGCTTTATTCCATGTACGGGCTCACCGAGTGCAAGCGCTGCACCTACCTGCCG CCGGAAGAGCTTGATCGGCGGCCCGATTCGGTCGGCATCGCCATCCCCAATACGGAAGCCTTTGTGGTGGATGATGACGG TAAGCCGGTCCCGCCCGGCGAGGTGGGCGAACTCGTCATCCGGGGCCCGCATGTCATGAAGGGCTATTGGCGCAACGACA AGGCGACGGTGAAGATGCTGCGCCCCGGCCTTAATCCGTGGGAAAAGGTGCTCTACACGGGCGATCTCTTCCGCATGGAT GCCGATGGCTTTCTCTACTTCGTCGGGCGCAAGGACGACATCATCAAAACGCGCGGCGAGAAGGTCGCGCCCAAGGAAGT GGAAGCGGTGCTTCACACCCATCCTTCCATTGCAGAGGCCGTCGTGGCGGGGGTTGTCGACCCGGTGCTCGGGCAGGCCA TCGCCGCCATGGTCGTCTCCAGGGATCCCGCGCTGACCGAGCGCGAGGTTATCCGCCACTGCTCGCAGCATCTGGAGGAT TTCATGGTACCCAAGATCGTGAAATTCGTCACTGAACTGCCGAAGACCGACACGGGCAAGGTGAGCCGCAGGCTCGCCGC CGAAACCATAGAGCCAGCCGAATGA
Upstream 100 bases:
>100_bases TTCCCGCCAATCTCGATTCCCTTGACCGTATCACAGCCTTCGTCAGCACGAAGACTGGCAAGGAATCGCTTAACGCCTGA GCGGAAGGCTGGAGGCCGGG
Downstream 100 bases:
>100_bases ACTCCACTGCGCCTTTTTCCGTCGCCACTCTCGCCATAGATCCCGCCGCCGAGGTGGAACGGATCGTGGCCGCTCTCCGC GAGCAGCTCCGCTCGCTGCG
Product: AMP-dependent synthetase and ligase
Products: NA
Alternate protein names: Long-chain acyl-CoA synthetase [H]
Number of amino acids: Translated: 514; Mature: 514
Protein sequence:
>514_residues MRVEEFLRGSASRYGEKTALVSGSTRLSYGEFDALTDRLAAALAANGVRRNDRVLIFMDNAWEAAVSIFAVLKAGATFSP INASTKAEKLAYIIGNCEAAAILTQAKLMPVAAEARALHGQPLLLISTEGRGGTVPDGTVSFTQCLEAEPAPVAHRGIDV DLAMLIYTSGSTGRPKGVMMTHRNIEAAATSITTYLRNRPDDIILNVLPLAFDYGLYQLLMSVKMGATLVLEKSFAFPHA IFDLMRKENVTGFPLVPTMAAMILQMRDLEPGFLPTLRYITNTAAALPPPHIARLRELCPGIELYSMYGLTECKRCTYLP PEELDRRPDSVGIAIPNTEAFVVDDDGKPVPPGEVGELVIRGPHVMKGYWRNDKATVKMLRPGLNPWEKVLYTGDLFRMD ADGFLYFVGRKDDIIKTRGEKVAPKEVEAVLHTHPSIAEAVVAGVVDPVLGQAIAAMVVSRDPALTEREVIRHCSQHLED FMVPKIVKFVTELPKTDTGKVSRRLAAETIEPAE
Sequences:
>Translated_514_residues MRVEEFLRGSASRYGEKTALVSGSTRLSYGEFDALTDRLAAALAANGVRRNDRVLIFMDNAWEAAVSIFAVLKAGATFSP INASTKAEKLAYIIGNCEAAAILTQAKLMPVAAEARALHGQPLLLISTEGRGGTVPDGTVSFTQCLEAEPAPVAHRGIDV DLAMLIYTSGSTGRPKGVMMTHRNIEAAATSITTYLRNRPDDIILNVLPLAFDYGLYQLLMSVKMGATLVLEKSFAFPHA IFDLMRKENVTGFPLVPTMAAMILQMRDLEPGFLPTLRYITNTAAALPPPHIARLRELCPGIELYSMYGLTECKRCTYLP PEELDRRPDSVGIAIPNTEAFVVDDDGKPVPPGEVGELVIRGPHVMKGYWRNDKATVKMLRPGLNPWEKVLYTGDLFRMD ADGFLYFVGRKDDIIKTRGEKVAPKEVEAVLHTHPSIAEAVVAGVVDPVLGQAIAAMVVSRDPALTEREVIRHCSQHLED FMVPKIVKFVTELPKTDTGKVSRRLAAETIEPAE >Mature_514_residues MRVEEFLRGSASRYGEKTALVSGSTRLSYGEFDALTDRLAAALAANGVRRNDRVLIFMDNAWEAAVSIFAVLKAGATFSP INASTKAEKLAYIIGNCEAAAILTQAKLMPVAAEARALHGQPLLLISTEGRGGTVPDGTVSFTQCLEAEPAPVAHRGIDV DLAMLIYTSGSTGRPKGVMMTHRNIEAAATSITTYLRNRPDDIILNVLPLAFDYGLYQLLMSVKMGATLVLEKSFAFPHA IFDLMRKENVTGFPLVPTMAAMILQMRDLEPGFLPTLRYITNTAAALPPPHIARLRELCPGIELYSMYGLTECKRCTYLP PEELDRRPDSVGIAIPNTEAFVVDDDGKPVPPGEVGELVIRGPHVMKGYWRNDKATVKMLRPGLNPWEKVLYTGDLFRMD ADGFLYFVGRKDDIIKTRGEKVAPKEVEAVLHTHPSIAEAVVAGVVDPVLGQAIAAMVVSRDPALTEREVIRHCSQHLED FMVPKIVKFVTELPKTDTGKVSRRLAAETIEPAE
Specific function: Involved in the degradation of long-chain fatty acids [H]
COG id: COG0318
COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II
Gene ontology:
Cell location: Partially Membrane-Associated [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]
Homologues:
Organism=Homo sapiens, GI187761345, Length=544, Percent_Identity=30.1470588235294, Blast_Score=186, Evalue=4e-47, Organism=Homo sapiens, GI187761343, Length=544, Percent_Identity=30.1470588235294, Blast_Score=186, Evalue=4e-47, Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=27.1897810218978, Blast_Score=160, Evalue=3e-39, Organism=Homo sapiens, GI42544132, Length=499, Percent_Identity=25.8517034068136, Blast_Score=158, Evalue=1e-38, Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=26.8924302788845, Blast_Score=155, Evalue=6e-38, Organism=Homo sapiens, GI157311624, Length=487, Percent_Identity=26.8993839835729, Blast_Score=146, Evalue=6e-35, Organism=Homo sapiens, GI157311622, Length=487, Percent_Identity=26.8993839835729, Blast_Score=146, Evalue=6e-35, Organism=Homo sapiens, GI58082049, Length=487, Percent_Identity=26.4887063655031, Blast_Score=143, Evalue=5e-34, Organism=Homo sapiens, GI122937307, Length=502, Percent_Identity=26.8924302788845, Blast_Score=142, Evalue=9e-34, Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=25.78125, Blast_Score=139, Evalue=4e-33, Organism=Homo sapiens, GI40807357, Length=547, Percent_Identity=25.0457038391225, Blast_Score=112, Evalue=7e-25, Organism=Homo sapiens, GI38524616, Length=536, Percent_Identity=25.3731343283582, Blast_Score=112, Evalue=1e-24, Organism=Homo sapiens, GI28416953, Length=523, Percent_Identity=25.0478011472275, Blast_Score=101, Evalue=1e-21, Organism=Homo sapiens, GI13375727, Length=551, Percent_Identity=22.8675136116152, Blast_Score=95, Evalue=1e-19, Organism=Homo sapiens, GI8923896, Length=545, Percent_Identity=22.9357798165138, Blast_Score=90, Evalue=6e-18, Organism=Homo sapiens, GI195947378, Length=414, Percent_Identity=23.6714975845411, Blast_Score=87, Evalue=4e-17, Organism=Homo sapiens, GI253970489, Length=558, Percent_Identity=22.5806451612903, Blast_Score=84, Evalue=4e-16, Organism=Homo sapiens, GI13236579, Length=479, Percent_Identity=25.4697286012526, Blast_Score=81, Evalue=2e-15, Organism=Homo sapiens, GI13325057, Length=387, Percent_Identity=26.0981912144703, Blast_Score=78, Evalue=2e-14, Organism=Escherichia coli, GI1788107, Length=530, Percent_Identity=29.4339622641509, Blast_Score=222, Evalue=5e-59, Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=30.7240704500978, Blast_Score=213, Evalue=2e-56, Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=27.4131274131274, Blast_Score=165, Evalue=6e-42, Organism=Escherichia coli, GI1786810, Length=508, Percent_Identity=26.1811023622047, Blast_Score=139, Evalue=6e-34, Organism=Escherichia coli, GI1786801, Length=454, Percent_Identity=25.9911894273128, Blast_Score=125, Evalue=7e-30, Organism=Escherichia coli, GI1790505, Length=536, Percent_Identity=24.4402985074627, Blast_Score=112, Evalue=5e-26, Organism=Escherichia coli, GI1786529, Length=528, Percent_Identity=24.8106060606061, Blast_Score=100, Evalue=3e-22, Organism=Escherichia coli, GI1789201, Length=345, Percent_Identity=27.536231884058, Blast_Score=93, Evalue=4e-20, Organism=Escherichia coli, GI1788595, Length=499, Percent_Identity=24.248496993988, Blast_Score=89, Evalue=6e-19, Organism=Caenorhabditis elegans, GI17531443, Length=483, Percent_Identity=27.7432712215321, Blast_Score=178, Evalue=5e-45, Organism=Caenorhabditis elegans, GI17557194, Length=499, Percent_Identity=27.0541082164329, Blast_Score=157, Evalue=1e-38, Organism=Caenorhabditis elegans, GI17559526, Length=520, Percent_Identity=27.1153846153846, Blast_Score=154, Evalue=1e-37, Organism=Caenorhabditis elegans, GI17538037, Length=527, Percent_Identity=26.3757115749526, Blast_Score=150, Evalue=1e-36, Organism=Caenorhabditis elegans, GI71994694, Length=526, Percent_Identity=26.9961977186312, Blast_Score=147, Evalue=2e-35, Organism=Caenorhabditis elegans, GI71994703, Length=526, Percent_Identity=26.9961977186312, Blast_Score=146, Evalue=2e-35, Organism=Caenorhabditis elegans, GI71994690, Length=526, Percent_Identity=26.9961977186312, Blast_Score=146, Evalue=3e-35, Organism=Caenorhabditis elegans, GI71996755, Length=508, Percent_Identity=26.1811023622047, Blast_Score=145, Evalue=3e-35, Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=27.56052141527, Blast_Score=142, Evalue=3e-34, Organism=Caenorhabditis elegans, GI32563687, Length=499, Percent_Identity=25.4509018036072, Blast_Score=139, Evalue=4e-33, Organism=Caenorhabditis elegans, GI17558820, Length=500, Percent_Identity=24.2, Blast_Score=127, Evalue=1e-29, Organism=Caenorhabditis elegans, GI115534440, Length=528, Percent_Identity=24.2424242424242, Blast_Score=121, Evalue=8e-28, Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=23.2824427480916, Blast_Score=119, Evalue=4e-27, Organism=Caenorhabditis elegans, GI17551278, Length=538, Percent_Identity=24.7211895910781, Blast_Score=119, Evalue=5e-27, Organism=Caenorhabditis elegans, GI71983001, Length=552, Percent_Identity=25.5434782608696, Blast_Score=111, Evalue=1e-24, Organism=Caenorhabditis elegans, GI71982997, Length=552, Percent_Identity=25.5434782608696, Blast_Score=110, Evalue=1e-24, Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=27.2988505747126, Blast_Score=106, Evalue=3e-23, Organism=Caenorhabditis elegans, GI25147511, Length=510, Percent_Identity=24.9019607843137, Blast_Score=105, Evalue=5e-23, Organism=Saccharomyces cerevisiae, GI6319699, Length=486, Percent_Identity=29.0123456790123, Blast_Score=192, Evalue=7e-50, Organism=Saccharomyces cerevisiae, GI6323182, Length=537, Percent_Identity=23.2774674115456, Blast_Score=114, Evalue=5e-26, Organism=Saccharomyces cerevisiae, GI6319264, Length=525, Percent_Identity=24.7619047619048, Blast_Score=108, Evalue=1e-24, Organism=Drosophila melanogaster, GI18859661, Length=531, Percent_Identity=28.8135593220339, Blast_Score=164, Evalue=1e-40, Organism=Drosophila melanogaster, GI24581924, Length=536, Percent_Identity=26.865671641791, Blast_Score=152, Evalue=6e-37, Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=27.7882797731569, Blast_Score=148, Evalue=7e-36, Organism=Drosophila melanogaster, GI24648260, Length=489, Percent_Identity=25.3578732106339, Blast_Score=139, Evalue=4e-33, Organism=Drosophila melanogaster, GI21356947, Length=498, Percent_Identity=25.3012048192771, Blast_Score=135, Evalue=8e-32, Organism=Drosophila melanogaster, GI21356441, Length=490, Percent_Identity=25.9183673469388, Blast_Score=130, Evalue=2e-30, Organism=Drosophila melanogaster, GI24653035, Length=474, Percent_Identity=23.6286919831224, Blast_Score=119, Evalue=5e-27, Organism=Drosophila melanogaster, GI24656500, Length=487, Percent_Identity=26.8993839835729, Blast_Score=116, Evalue=3e-26, Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=24.040404040404, Blast_Score=116, Evalue=4e-26, Organism=Drosophila melanogaster, GI161076582, Length=481, Percent_Identity=23.7006237006237, Blast_Score=112, Evalue=4e-25, Organism=Drosophila melanogaster, GI24648253, Length=468, Percent_Identity=25.4273504273504, Blast_Score=112, Evalue=9e-25, Organism=Drosophila melanogaster, GI24648255, Length=468, Percent_Identity=25.4273504273504, Blast_Score=109, Evalue=3e-24, Organism=Drosophila melanogaster, GI20130357, Length=506, Percent_Identity=25.098814229249, Blast_Score=109, Evalue=5e-24, Organism=Drosophila melanogaster, GI24648676, Length=471, Percent_Identity=27.3885350318471, Blast_Score=108, Evalue=1e-23, Organism=Drosophila melanogaster, GI62472339, Length=537, Percent_Identity=23.6499068901304, Blast_Score=106, Evalue=3e-23, Organism=Drosophila melanogaster, GI24648257, Length=502, Percent_Identity=25.0996015936255, Blast_Score=105, Evalue=7e-23, Organism=Drosophila melanogaster, GI24667955, Length=537, Percent_Identity=23.6499068901304, Blast_Score=105, Evalue=7e-23, Organism=Drosophila melanogaster, GI281365686, Length=305, Percent_Identity=26.5573770491803, Blast_Score=101, Evalue=1e-21, Organism=Drosophila melanogaster, GI21358303, Length=331, Percent_Identity=25.6797583081571, Blast_Score=100, Evalue=4e-21, Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=24.6031746031746, Blast_Score=93, Evalue=4e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR020459 - InterPro: IPR020845 - InterPro: IPR000873 [H]
Pfam domain/function: PF00501 AMP-binding [H]
EC number: =6.2.1.3 [H]
Molecular weight: Translated: 56186; Mature: 56186
Theoretical pI: Translated: 6.52; Mature: 6.52
Prosite motif: PS00455 AMP_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 3.5 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRVEEFLRGSASRYGEKTALVSGSTRLSYGEFDALTDRLAAALAANGVRRNDRVLIFMDN CCHHHHHCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC AWEAAVSIFAVLKAGATFSPINASTKAEKLAYIIGNCEAAAILTQAKLMPVAAEARALHG CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEECCCHHHHHHHHHHHCCHHHHHHHCCC QPLLLISTEGRGGTVPDGTVSFTQCLEAEPAPVAHRGIDVDLAMLIYTSGSTGRPKGVMM CEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCCCCCCCCEEE THRNIEAAATSITTYLRNRPDDIILNVLPLAFDYGLYQLLMSVKMGATLVLEKSFAFPHA ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH IFDLMRKENVTGFPLVPTMAAMILQMRDLEPGFLPTLRYITNTAAALPPPHIARLRELCP HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCC GIELYSMYGLTECKRCTYLPPEELDRRPDSVGIAIPNTEAFVVDDDGKPVPPGEVGELVI CCEEHHHHCCHHHCCCCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCEEE RGPHVMKGYWRNDKATVKMLRPGLNPWEKVLYTGDLFRMDADGFLYFVGRKDDIIKTRGE ECCHHHCCEECCCHHHHHHHCCCCCHHHHHHCCCCEEEECCCCEEEEECCCCCHHHCCCC KVAPKEVEAVLHTHPSIAEAVVAGVVDPVLGQAIAAMVVSRDPALTEREVIRHCSQHLED CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH FMVPKIVKFVTELPKTDTGKVSRRLAAETIEPAE HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC >Mature Secondary Structure MRVEEFLRGSASRYGEKTALVSGSTRLSYGEFDALTDRLAAALAANGVRRNDRVLIFMDN CCHHHHHCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC AWEAAVSIFAVLKAGATFSPINASTKAEKLAYIIGNCEAAAILTQAKLMPVAAEARALHG CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEECCCHHHHHHHHHHHCCHHHHHHHCCC QPLLLISTEGRGGTVPDGTVSFTQCLEAEPAPVAHRGIDVDLAMLIYTSGSTGRPKGVMM CEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCCCCCCCCEEE THRNIEAAATSITTYLRNRPDDIILNVLPLAFDYGLYQLLMSVKMGATLVLEKSFAFPHA ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH IFDLMRKENVTGFPLVPTMAAMILQMRDLEPGFLPTLRYITNTAAALPPPHIARLRELCP HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCC GIELYSMYGLTECKRCTYLPPEELDRRPDSVGIAIPNTEAFVVDDDGKPVPPGEVGELVI CCEEHHHHCCHHHCCCCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCEEE RGPHVMKGYWRNDKATVKMLRPGLNPWEKVLYTGDLFRMDADGFLYFVGRKDDIIKTRGE ECCHHHCCEECCCHHHHHHHCCCCCHHHHHHCCCCEEEECCCCEEEEECCCCCHHHCCCC KVAPKEVEAVLHTHPSIAEAVVAGVVDPVLGQAIAAMVVSRDPALTEREVIRHCSQHLED CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH FMVPKIVKFVTELPKTDTGKVSRRLAAETIEPAE HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9579061; 9384377 [H]