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The map label for this gene is nadE [H]

Identifier: 110635214

GI number: 110635214

Start: 3134042

End: 3135034

Strand: Reverse

Name: nadE [H]

Synonym: Meso_2881

Alternate gene names: 110635214

Gene position: 3135034-3134042 (Counterclockwise)

Preceding gene: 110635215

Following gene: 110635204

Centisome position: 71.05

GC content: 62.24

Gene sequence:

>993_bases
ATGAACTCCACTGCGCCTTTTTCCGTCGCCACTCTCGCCATAGATCCCGCCGCCGAGGTGGAACGGATCGTGGCCGCTCT
CCGCGAGCAGCTCCGCTCGCTGCGCAAGCGTGGGCTGGTGCTGGGCGTCTCCGGCGGCATCGATTCCAGCGTTTCCACCG
CGCTCGCAGTCAGAGCAGTCGGCGCGAAGAATGTCTGCTGCCTGTTCATGCCCGAGAATGATTCCGATCCCGAGAGCCTT
GAGCTCGGCCGGCTGCTCACGAACACCTTCGGGATTGAAGGCATCGTGGAAGATATTGGTCCTGCGCTCAGAGCGATTGG
TTGCTATGAGCGGCGCGACGGCTTCATCCGCAAGCTCGTGCCGGACTACGGTCCCGGCTGGGCGTCCAAGATCGTCATCT
CCAACGCGCTTGAAGGTGCGGCCTACAACATCTCCTGGCTTGTGGTGCAGGACCCAGAGGGGAACCAGAGCAGGCATCGG
ATGACGCCGGAGGTCTATCTCGGCATTGTCGCCGCCACCAATATGAAGCAGCGCACGCGCAAGCAGATCGAGTATTACCA
CGCCGACCGGCTGAACTTCGCCGTGCTCGGCACGCCGAACCGGCTGGAATACGATCAGGGCTTCTTCGTTAAGAACGGCG
ATGGTGCTGCCGATGTGAAGCCCATCGCACATCTTTATAAGTCACAGGTCTACCAGCTTGCCGAATATCTCGGCGTGCCG
GAGGTGATCTGCCGCCGCCCGCCGACGACCGACACCTATTCGCTCGCCCAGACGCAGGAGGAATTCTACTTCTCGCTGCC
CTATGACCGGATGGACCTCTGCCTCTACGGCCTCAACCATGGCCTTCCGGCGAGCGCCGTTGCCGCGGCGGCCTCACTCA
CCGAAGAGCAGTTGCAGAGTGTATGGGCTGATGTCGCCGCGAAGCGCAAGGCGACGCGCTATCTTCACCTCCCGCCGCTG
CTCGTCGATAAGGTGTCAGAGGTCGGCGCCTGA

Upstream 100 bases:

>100_bases
TCATGGTACCCAAGATCGTGAAATTCGTCACTGAACTGCCGAAGACCGACACGGGCAAGGTGAGCCGCAGGCTCGCCGCC
GAAACCATAGAGCCAGCCGA

Downstream 100 bases:

>100_bases
AAAGCTACGCTAGGGGAGGCCGCTCAACCTTCGTTCAGCTTTCGGCTTTCAACCCATGCCTTCGGATCGACACGTGAGCG
GAGAAACCCGACGGGTAGCG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNSTAPFSVATLAIDPAAEVERIVAALREQLRSLRKRGLVLGVSGGIDSSVSTALAVRAVGAKNVCCLFMPENDSDPESL
ELGRLLTNTFGIEGIVEDIGPALRAIGCYERRDGFIRKLVPDYGPGWASKIVISNALEGAAYNISWLVVQDPEGNQSRHR
MTPEVYLGIVAATNMKQRTRKQIEYYHADRLNFAVLGTPNRLEYDQGFFVKNGDGAADVKPIAHLYKSQVYQLAEYLGVP
EVICRRPPTTDTYSLAQTQEEFYFSLPYDRMDLCLYGLNHGLPASAVAAAASLTEEQLQSVWADVAAKRKATRYLHLPPL
LVDKVSEVGA

Sequences:

>Translated_330_residues
MNSTAPFSVATLAIDPAAEVERIVAALREQLRSLRKRGLVLGVSGGIDSSVSTALAVRAVGAKNVCCLFMPENDSDPESL
ELGRLLTNTFGIEGIVEDIGPALRAIGCYERRDGFIRKLVPDYGPGWASKIVISNALEGAAYNISWLVVQDPEGNQSRHR
MTPEVYLGIVAATNMKQRTRKQIEYYHADRLNFAVLGTPNRLEYDQGFFVKNGDGAADVKPIAHLYKSQVYQLAEYLGVP
EVICRRPPTTDTYSLAQTQEEFYFSLPYDRMDLCLYGLNHGLPASAVAAAASLTEEQLQSVWADVAAKRKATRYLHLPPL
LVDKVSEVGA
>Mature_330_residues
MNSTAPFSVATLAIDPAAEVERIVAALREQLRSLRKRGLVLGVSGGIDSSVSTALAVRAVGAKNVCCLFMPENDSDPESL
ELGRLLTNTFGIEGIVEDIGPALRAIGCYERRDGFIRKLVPDYGPGWASKIVISNALEGAAYNISWLVVQDPEGNQSRHR
MTPEVYLGIVAATNMKQRTRKQIEYYHADRLNFAVLGTPNRLEYDQGFFVKNGDGAADVKPIAHLYKSQVYQLAEYLGVP
EVICRRPPTTDTYSLAQTQEEFYFSLPYDRMDLCLYGLNHGLPASAVAAAASLTEEQLQSVWADVAAKRKATRYLHLPPL
LVDKVSEVGA

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 36195; Mature: 36195

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTAPFSVATLAIDPAAEVERIVAALREQLRSLRKRGLVLGVSGGIDSSVSTALAVRAV
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCC
GAKNVCCLFMPENDSDPESLELGRLLTNTFGIEGIVEDIGPALRAIGCYERRDGFIRKLV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
PDYGPGWASKIVISNALEGAAYNISWLVVQDPEGNQSRHRMTPEVYLGIVAATNMKQRTR
CCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHCCCHHHHEEEHHHCCHHHHHH
KQIEYYHADRLNFAVLGTPNRLEYDQGFFVKNGDGAADVKPIAHLYKSQVYQLAEYLGVP
HHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
EVICRRPPTTDTYSLAQTQEEFYFSLPYDRMDLCLYGLNHGLPASAVAAAASLTEEQLQS
HHHHCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VWADVAAKRKATRYLHLPPLLVDKVSEVGA
HHHHHHHHHHHCCEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MNSTAPFSVATLAIDPAAEVERIVAALREQLRSLRKRGLVLGVSGGIDSSVSTALAVRAV
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCC
GAKNVCCLFMPENDSDPESLELGRLLTNTFGIEGIVEDIGPALRAIGCYERRDGFIRKLV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
PDYGPGWASKIVISNALEGAAYNISWLVVQDPEGNQSRHRMTPEVYLGIVAATNMKQRTR
CCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHCCCHHHHEEEHHHCCHHHHHH
KQIEYYHADRLNFAVLGTPNRLEYDQGFFVKNGDGAADVKPIAHLYKSQVYQLAEYLGVP
HHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
EVICRRPPTTDTYSLAQTQEEFYFSLPYDRMDLCLYGLNHGLPASAVAAAASLTEEQLQS
HHHHCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VWADVAAKRKATRYLHLPPLLVDKVSEVGA
HHHHHHHHHHHCCEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]