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The map label for this gene is yfcB [C]

Identifier: 110635204

GI number: 110635204

Start: 3122704

End: 3123408

Strand: Reverse

Name: yfcB [C]

Synonym: Meso_2871

Alternate gene names: 110635204

Gene position: 3123408-3122704 (Counterclockwise)

Preceding gene: 110635214

Following gene: 110635203

Centisome position: 70.79

GC content: 63.83

Gene sequence:

>705_bases
ATGGCAAAACCAACCAGAACCGATGCGGATATCCAGGCCGGCGCGCCGCACCCGAAAACCGCCCGTTTCATGGGCATCGA
GCTGGAACTGGCGCCGGATGTATTGGTGCCGAGGGAGGAAACGGAGCTTCTGGGCCGGAGCGCCGTCGATTTGTTGAAGG
GCATGGCCGGTTCGCCGATCGTGATCGACATGTGCTGCGGCTCCGGCAATCTTGCCCTCGGCATTGCGGCTGCTGTTCCT
GCTGCACGCCTATGGGCTTCCGACCTCACCGAAAGCACCGTTTCCCTGGCGCGGCGCAATGCAGAACGGCTCGGCCTCCT
CGATCGCGTAAAGGTCGTGCAGGGCGACCTCTTTTCCGGCTTGGCAGACGAGGGGCTGGAAGGGCGGGTGGACTTCGTCG
TGAGCAATCCACCCTATATCTCGACCTCGAGGCTGGAGACCGACCGCGCCCACCTGCTTGAGAACGAGCCGAGGGAAGCG
TTCGACGGCGGCCCCTACGGTCTCTCGATCCATCAGCGTCTGGTGCGTGAGGCACCTACGTTCCTCAAGCGCGGAGGATG
GCTCGCCTTCGAATTCGGCGAAGGGCAGGAGCGCCAGGTCGCGATCCTCTTGAAGCGCGCCGGGATGTATGAGGAACCGC
GCTTTGCTTCCGACCAGGCGGGTAAGCCGCGCGTTGCGATTGCGCGCAGGAAGCAGGAGCCATGA

Upstream 100 bases:

>100_bases
TCTCTGAAACCGCTTCCGGGCCGACACGCTAGGAGTTTCAAGCTCCATTTACCTCTGTGATCTATATCCACTTTCACCAT
TCCGGGGAGGCGAGCGCCGC

Downstream 100 bases:

>100_bases
CGGCTATCCGCCCTCTGGAGAAGGCGGATATCCCGGCTGTAGCCGGTCTTTTCCAGCGCATTTTCCGTAACGCCGGGCGG
CCCCCTCCGGCGTCGCTTAC

Product: HemK family modification methylase

Products: S-adenosyl-L-homocysteine; DNA methylaminopurine [C]

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MAKPTRTDADIQAGAPHPKTARFMGIELELAPDVLVPREETELLGRSAVDLLKGMAGSPIVIDMCCGSGNLALGIAAAVP
AARLWASDLTESTVSLARRNAERLGLLDRVKVVQGDLFSGLADEGLEGRVDFVVSNPPYISTSRLETDRAHLLENEPREA
FDGGPYGLSIHQRLVREAPTFLKRGGWLAFEFGEGQERQVAILLKRAGMYEEPRFASDQAGKPRVAIARRKQEP

Sequences:

>Translated_234_residues
MAKPTRTDADIQAGAPHPKTARFMGIELELAPDVLVPREETELLGRSAVDLLKGMAGSPIVIDMCCGSGNLALGIAAAVP
AARLWASDLTESTVSLARRNAERLGLLDRVKVVQGDLFSGLADEGLEGRVDFVVSNPPYISTSRLETDRAHLLENEPREA
FDGGPYGLSIHQRLVREAPTFLKRGGWLAFEFGEGQERQVAILLKRAGMYEEPRFASDQAGKPRVAIARRKQEP
>Mature_233_residues
AKPTRTDADIQAGAPHPKTARFMGIELELAPDVLVPREETELLGRSAVDLLKGMAGSPIVIDMCCGSGNLALGIAAAVPA
ARLWASDLTESTVSLARRNAERLGLLDRVKVVQGDLFSGLADEGLEGRVDFVVSNPPYISTSRLETDRAHLLENEPREAF
DGGPYGLSIHQRLVREAPTFLKRGGWLAFEFGEGQERQVAILLKRAGMYEEPRFASDQAGKPRVAIARRKQEP

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=218, Percent_Identity=31.651376146789, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87082085, Length=178, Percent_Identity=30.3370786516854, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787463, Length=215, Percent_Identity=30.6976744186047, Blast_Score=77, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24582226, Length=206, Percent_Identity=30.0970873786408, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.72 [C]

Molecular weight: Translated: 25442; Mature: 25311

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPTRTDADIQAGAPHPKTARFMGIELELAPDVLVPREETELLGRSAVDLLKGMAGSPI
CCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
VIDMCCGSGNLALGIAAAVPAARLWASDLTESTVSLARRNAERLGLLDRVKVVQGDLFSG
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHH
LADEGLEGRVDFVVSNPPYISTSRLETDRAHLLENEPREAFDGGPYGLSIHQRLVREAPT
HHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHH
FLKRGGWLAFEFGEGQERQVAILLKRAGMYEEPRFASDQAGKPRVAIARRKQEP
HHHCCCEEEEECCCCCCHHHHHEEHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
AKPTRTDADIQAGAPHPKTARFMGIELELAPDVLVPREETELLGRSAVDLLKGMAGSPI
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
VIDMCCGSGNLALGIAAAVPAARLWASDLTESTVSLARRNAERLGLLDRVKVVQGDLFSG
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHH
LADEGLEGRVDFVVSNPPYISTSRLETDRAHLLENEPREAFDGGPYGLSIHQRLVREAPT
HHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHH
FLKRGGWLAFEFGEGQERQVAILLKRAGMYEEPRFASDQAGKPRVAIARRKQEP
HHHCCCEEEEECCCCCCHHHHHEEHHCCCCCCCCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; DNA adenine; DNA [C]

Specific reaction: S-Adenosyl-L-methionine + DNA adenine =S-adenosyl-L-homocysteine + DNA methylaminopurine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]