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The map label for this gene is yoaE [H]

Identifier: 110634319

GI number: 110634319

Start: 2110480

End: 2112591

Strand: Reverse

Name: yoaE [H]

Synonym: Meso_1969

Alternate gene names: 110634319

Gene position: 2112591-2110480 (Counterclockwise)

Preceding gene: 110634323

Following gene: 110634315

Centisome position: 47.88

GC content: 64.91

Gene sequence:

>2112_bases
ATGAACACACACTCATCGCTTCATATCGGCCATTCGGCCTGTCCGCATGACTGTCCTTCCACCTGCGCGCTCGATGTCGA
GGTGATCGCCGGGCGCATCGGCCGCGTCCACGGCGCCAAGGACAACAGCTATACCGCCGGCGTCATCTGCGCCAAGGTGG
CGCGCTATGGCGAGCGCGTGCATCATCCCGACCGCCTTCTGAAGCCTCTGGTGCGCGCGGGCGCCAAAGGGGAGGGTGTG
TGGAAGGAGGCGAGCTGGGAGGCGGCGCTCGACCGCTTGGCGGAAGCTTTTCTGGAGGCTGAAGCGAATTACGGCAGCGA
GAGCGTCTGGCCCTATCATTATGCGGGCACGATGGGCCTGGTGCAGCGCGATTCCATCCATCGCCTGCGCCACGCCAAGC
GTTATTCCGGGCAGTTCGACACGATTTGCACAAACCCCGCCTGGGCGGGCTATGTGATGGGCACTGGCGCGCTTCGCGGT
GCCGATCCGCGCGAGATGGCCAAGGCCGATTGCGTGGTCATCTGGGGCACCAATGCGGTGGCGACCCAAGTGAATGTGAT
GACGCATGCCGTGCGCGCCCGCAAGGAGCGTGGGGCGAAGATCGTCGTCATAGACGTCTATGACAACGCCACCATGAAGC
AGGCCGATCTCGGCATCATCGTCCGGCCGGGCACGGATGGGGCGCTCGCCTGCGCCGTCATGCATGTGCTCTTCCGCGAT
GGCTTGGTCGACCGGCCGTATCTGGAAAGATATACGGACGATCCCAAAGGGCTGGAACAGCATCTTTCCACGCGCACGCC
GGAATGGGCGGAAGCCATCACTGGCGTTCCGGCGGCTGAGATCGAGGCTTTCGCCCGGCTGGTGGGCGAGACGAAGCGCA
CCTTTTTCCGCCTGGGTTACGGTTTCACCCGCAACCGGAACGGCGCGGTTAACATGCATGCCGCCCTTTCGATCGCCGCC
GTTACCGGCGCGTGGCAATATGAAGGCGGCGGCGCCTTCCATTCCAATTCCGGCATTTTCGGCCTCGACAAGTCGCTGAT
CGAGGGGACAACCTTTGCCGATCCGTCGGTGCGCTCGCTCGATCAGTCGCAGATCGGCCGGGTGCTGACCGGCGATGAGG
CGGCGCTTCACGGCGGGCCGCCGGTCACGGCGATGCTCATCCAGAATACCAATCCCGCCAATGTCGCGCCGGAGCAGCGG
CTCGTGAAGCGCGGCTTCCTGCGCGAGGATCTCTTCGTGTGCGTGCACGAACAGTTCATGACCGACACGGCGAAGCTCGC
CGACGTGGTGCTGCCCGCCACGATGTTCGTGGAGCATGACGACATTTATCGCGGCGGCGGCCATCAGCACATCCTGCTCG
GGCCCAAGCTGATCGACGCGCCCGGTGAAGCGCGCGAGAACATCTTCGTCATCGACGAGCTCGCGAAGCGGCTTGGCGTG
GCGCATCTGCCCGGCTTCGGCCTTTCGGCGCGCGAGCATCTGGACTGGACGCTCGGCCGAAGGGGGCTTGGCACTTTCGA
CAGTTTCCGCCACGAGCGCTGGGCCGACGTGCAGCCGGATTTCGAGACGGCGCATTTCCTTACCGGCTTCGGCCATCCCG
ACGGGAAATACCGCTTCCGCCCGAGCTGGAAGGGGACGCCCGGGCCGAACCATCCGCCCGAATCGATCGGCTTCCAAGGA
CCGCACGAGAGCCTGCCCGAATTCCCCGATCATGTGGATTTCATCGAGGCGGCCGATCCCGAGCATCCCTTCCGTCTTGC
CACATCGCCGGCGCGCAGCTTCCTGAATTCAAGCTTCACGGAAACGCCGGGCTCGAAGAAGCGGGAGGGCAGGCCTGAAC
TGATGTTGCATCCGGAGGATGCGCTGGCGCTCGGGATCGCCGAGGGTGACCGGGTGGAGATCGGCAACGGCCGCGGCGAA
GTGGTGCTCCACGCCAAGATCTTTGACGGAGTTCGCCGGGGCGTGGTTATTGCCGAAGGCATCTGGCCGAACGGCGCGCA
TGAGCGCGGCGAGGGCATCAATGTTTTGACGGGCGCCGATGCCGTGGCCCCCTACGGGGGCGCTGCCTTCCACGACACAA
AGATCTGGGTGAGGGCGGCTCACGCGGCGTGA

Upstream 100 bases:

>100_bases
CGTCCGGCTGAAATCCATCATCCTGACTGATGGATCATGTAAGCGCTTTTCACGGGTTTGGCCCTACTCCGATGAGGGCA
AAACCGCTAGTCTCCAGCGC

Downstream 100 bases:

>100_bases
TCCGCCCTTATCCGGCCATGCGGATCTAATCTCTGCTTCTGGTTATTGGCCGCTGTAAAGCTGAAATGCGTCTTGCCGCA
GGGCGTGGCGCGAATCTGCT

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MNTHSSLHIGHSACPHDCPSTCALDVEVIAGRIGRVHGAKDNSYTAGVICAKVARYGERVHHPDRLLKPLVRAGAKGEGV
WKEASWEAALDRLAEAFLEAEANYGSESVWPYHYAGTMGLVQRDSIHRLRHAKRYSGQFDTICTNPAWAGYVMGTGALRG
ADPREMAKADCVVIWGTNAVATQVNVMTHAVRARKERGAKIVVIDVYDNATMKQADLGIIVRPGTDGALACAVMHVLFRD
GLVDRPYLERYTDDPKGLEQHLSTRTPEWAEAITGVPAAEIEAFARLVGETKRTFFRLGYGFTRNRNGAVNMHAALSIAA
VTGAWQYEGGGAFHSNSGIFGLDKSLIEGTTFADPSVRSLDQSQIGRVLTGDEAALHGGPPVTAMLIQNTNPANVAPEQR
LVKRGFLREDLFVCVHEQFMTDTAKLADVVLPATMFVEHDDIYRGGGHQHILLGPKLIDAPGEARENIFVIDELAKRLGV
AHLPGFGLSAREHLDWTLGRRGLGTFDSFRHERWADVQPDFETAHFLTGFGHPDGKYRFRPSWKGTPGPNHPPESIGFQG
PHESLPEFPDHVDFIEAADPEHPFRLATSPARSFLNSSFTETPGSKKREGRPELMLHPEDALALGIAEGDRVEIGNGRGE
VVLHAKIFDGVRRGVVIAEGIWPNGAHERGEGINVLTGADAVAPYGGAAFHDTKIWVRAAHAA

Sequences:

>Translated_703_residues
MNTHSSLHIGHSACPHDCPSTCALDVEVIAGRIGRVHGAKDNSYTAGVICAKVARYGERVHHPDRLLKPLVRAGAKGEGV
WKEASWEAALDRLAEAFLEAEANYGSESVWPYHYAGTMGLVQRDSIHRLRHAKRYSGQFDTICTNPAWAGYVMGTGALRG
ADPREMAKADCVVIWGTNAVATQVNVMTHAVRARKERGAKIVVIDVYDNATMKQADLGIIVRPGTDGALACAVMHVLFRD
GLVDRPYLERYTDDPKGLEQHLSTRTPEWAEAITGVPAAEIEAFARLVGETKRTFFRLGYGFTRNRNGAVNMHAALSIAA
VTGAWQYEGGGAFHSNSGIFGLDKSLIEGTTFADPSVRSLDQSQIGRVLTGDEAALHGGPPVTAMLIQNTNPANVAPEQR
LVKRGFLREDLFVCVHEQFMTDTAKLADVVLPATMFVEHDDIYRGGGHQHILLGPKLIDAPGEARENIFVIDELAKRLGV
AHLPGFGLSAREHLDWTLGRRGLGTFDSFRHERWADVQPDFETAHFLTGFGHPDGKYRFRPSWKGTPGPNHPPESIGFQG
PHESLPEFPDHVDFIEAADPEHPFRLATSPARSFLNSSFTETPGSKKREGRPELMLHPEDALALGIAEGDRVEIGNGRGE
VVLHAKIFDGVRRGVVIAEGIWPNGAHERGEGINVLTGADAVAPYGGAAFHDTKIWVRAAHAA
>Mature_703_residues
MNTHSSLHIGHSACPHDCPSTCALDVEVIAGRIGRVHGAKDNSYTAGVICAKVARYGERVHHPDRLLKPLVRAGAKGEGV
WKEASWEAALDRLAEAFLEAEANYGSESVWPYHYAGTMGLVQRDSIHRLRHAKRYSGQFDTICTNPAWAGYVMGTGALRG
ADPREMAKADCVVIWGTNAVATQVNVMTHAVRARKERGAKIVVIDVYDNATMKQADLGIIVRPGTDGALACAVMHVLFRD
GLVDRPYLERYTDDPKGLEQHLSTRTPEWAEAITGVPAAEIEAFARLVGETKRTFFRLGYGFTRNRNGAVNMHAALSIAA
VTGAWQYEGGGAFHSNSGIFGLDKSLIEGTTFADPSVRSLDQSQIGRVLTGDEAALHGGPPVTAMLIQNTNPANVAPEQR
LVKRGFLREDLFVCVHEQFMTDTAKLADVVLPATMFVEHDDIYRGGGHQHILLGPKLIDAPGEARENIFVIDELAKRLGV
AHLPGFGLSAREHLDWTLGRRGLGTFDSFRHERWADVQPDFETAHFLTGFGHPDGKYRFRPSWKGTPGPNHPPESIGFQG
PHESLPEFPDHVDFIEAADPEHPFRLATSPARSFLNSSFTETPGSKKREGRPELMLHPEDALALGIAEGDRVEIGNGRGE
VVLHAKIFDGVRRGVVIAEGIWPNGAHERGEGINVLTGADAVAPYGGAAFHDTKIWVRAAHAA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=767, Percent_Identity=27.509778357236, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI171474008, Length=741, Percent_Identity=25.1012145748988, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI1787870, Length=525, Percent_Identity=27.2380952380952, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI3868721, Length=704, Percent_Identity=25.2840909090909, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081994, Length=749, Percent_Identity=24.9666221628838, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI145693196, Length=768, Percent_Identity=26.0416666666667, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI3868720, Length=414, Percent_Identity=24.8792270531401, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 76562; Mature: 76562

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHSSLHIGHSACPHDCPSTCALDVEVIAGRIGRVHGAKDNSYTAGVICAKVARYGERV
CCCCCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHC
HHPDRLLKPLVRAGAKGEGVWKEASWEAALDRLAEAFLEAEANYGSESVWPYHYAGTMGL
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCH
VQRDSIHRLRHAKRYSGQFDTICTNPAWAGYVMGTGALRGADPREMAKADCVVIWGTNAV
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEECCCCCCCCHHHHCCCCEEEEECCCHH
ATQVNVMTHAVRARKERGAKIVVIDVYDNATMKQADLGIIVRPGTDGALACAVMHVLFRD
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHC
GLVDRPYLERYTDDPKGLEQHLSTRTPEWAEAITGVPAAEIEAFARLVGETKRTFFRLGY
CCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
GFTRNRNGAVNMHAALSIAAVTGAWQYEGGGAFHSNSGIFGLDKSLIEGTTFADPSVRSL
CEECCCCCCEEEHHHEEEEEEECCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCCHHHC
DQSQIGRVLTGDEAALHGGPPVTAMLIQNTNPANVAPEQRLVKRGFLREDLFVCVHEQFM
CHHHCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TDTAKLADVVLPATMFVEHDDIYRGGGHQHILLGPKLIDAPGEARENIFVIDELAKRLGV
HHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCEEECCCCCCCCCEEEHHHHHHHCCC
AHLPGFGLSAREHLDWTLGRRGLGTFDSFRHERWADVQPDFETAHFLTGFGHPDGKYRFR
CCCCCCCCCHHHHCCHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHEEECCCCCCCEEEC
PSWKGTPGPNHPPESIGFQGPHESLPEFPDHVDFIEAADPEHPFRLATSPARSFLNSSFT
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCEEEECHHHHHHHHCCCC
ETPGSKKREGRPELMLHPEDALALGIAEGDRVEIGNGRGEVVLHAKIFDGVRRGVVIAEG
CCCCCCCCCCCCCEEECCCCCEEEEECCCCEEEECCCCCCEEEEEHHHHHHHCCEEEEEC
IWPNGAHERGEGINVLTGADAVAPYGGAAFHDTKIWVRAAHAA
CCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCEEEEEEECCC
>Mature Secondary Structure
MNTHSSLHIGHSACPHDCPSTCALDVEVIAGRIGRVHGAKDNSYTAGVICAKVARYGERV
CCCCCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHC
HHPDRLLKPLVRAGAKGEGVWKEASWEAALDRLAEAFLEAEANYGSESVWPYHYAGTMGL
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCH
VQRDSIHRLRHAKRYSGQFDTICTNPAWAGYVMGTGALRGADPREMAKADCVVIWGTNAV
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEECCCCCCCCHHHHCCCCEEEEECCCHH
ATQVNVMTHAVRARKERGAKIVVIDVYDNATMKQADLGIIVRPGTDGALACAVMHVLFRD
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHC
GLVDRPYLERYTDDPKGLEQHLSTRTPEWAEAITGVPAAEIEAFARLVGETKRTFFRLGY
CCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
GFTRNRNGAVNMHAALSIAAVTGAWQYEGGGAFHSNSGIFGLDKSLIEGTTFADPSVRSL
CEECCCCCCEEEHHHEEEEEEECCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCCHHHC
DQSQIGRVLTGDEAALHGGPPVTAMLIQNTNPANVAPEQRLVKRGFLREDLFVCVHEQFM
CHHHCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TDTAKLADVVLPATMFVEHDDIYRGGGHQHILLGPKLIDAPGEARENIFVIDELAKRLGV
HHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCEEECCCCCCCCCEEEHHHHHHHCCC
AHLPGFGLSAREHLDWTLGRRGLGTFDSFRHERWADVQPDFETAHFLTGFGHPDGKYRFR
CCCCCCCCCHHHHCCHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHEEECCCCCCCEEEC
PSWKGTPGPNHPPESIGFQGPHESLPEFPDHVDFIEAADPEHPFRLATSPARSFLNSSFT
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCEEEECHHHHHHHHCCCC
ETPGSKKREGRPELMLHPEDALALGIAEGDRVEIGNGRGEVVLHAKIFDGVRRGVVIAEG
CCCCCCCCCCCCCEEECCCCCEEEEECCCCEEEECCCCCCEEEEEHHHHHHHCCEEEEEC
IWPNGAHERGEGINVLTGADAVAPYGGAAFHDTKIWVRAAHAA
CCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]