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The map label for this gene is gsiB [H]

Identifier: 110634300

GI number: 110634300

Start: 2090851

End: 2092371

Strand: Direct

Name: gsiB [H]

Synonym: Meso_1950

Alternate gene names: 110634300

Gene position: 2090851-2092371 (Clockwise)

Preceding gene: 110634299

Following gene: 110634301

Centisome position: 47.39

GC content: 64.63

Gene sequence:

>1521_bases
ATGATCAACCTGTCTAAATCCGGCCTGGCCTGGACCTTGCTGGCTGCGCAGGCCGGACTTGCCTTCGCGGCAGCACCGGC
CCTCACCGCCGAAGGGTCCATCGTCCTGGCGATGCCGGCCGAGCCGACCTCGGTCGATGCCTGCGATGACAGCACTAGGG
CCAATGCCCGGGTGCTGCGCGGCAATGTCGTCGAGGCGCTGACGCGGCTCGACCCACAGTCGGGCGCGGTCGGCCCGCTG
CTCGCCACCGAATGGTCTAGTCCGGATAACAAGAGCTGGCTCTTCACCATCCGGCCGGGCGTCACCTTCCACGACGGCAC
GCCGCTGGATGCGGCCGCGGTGGCGTTCGGCATCAACCGCTCGATGAACCCGGACCTGACGTGCCAGACCCTGTCGCTGT
TCCCCACCAAGACCACCGCGACCGTCGAAAGCGACATGGTGGTACGGATCACCACCGAAGAGCCCGACCCGATCCTGCCG
GCACGCATCGCCTATATCGATCTGCCCTCCCCCAAGACTCCGGAAGCCGCCAAAAGTGACACCCCGATCGGCACCGGTCC
CTACCGGTTTGCCGGCCGCGAGATCGGCCAGTCGATAACGCTATCGGCGTTTGACGGCTATTGGGGCGAAGCCCCCGAGA
TCGCCGAAGCCAACTATGTCTGGCGGGCCGAGGCCACCATCCGCGCCAGCATGATCAAGACCGGCGAAGCCGATATCGCC
TATGATATCCCCACCCATGAGGCCGAAGGCCAGGCCAATGCCCAGCAATATTTGACCAACGGCGTGTTCTACCTGCGCCC
GATGCTGCAGAAGCCGCCGCTGGACGATCTGCGCGTGCGCCAGGCCATCGCCTCCTCGATAGACAAGGCCACGCTGGCCG
AAGTGCTGATGGACAATTCGGGCACGCCGACCGGGCAATTGGTCACACAGCTGATCAATGGCTACGTGCCCGATTATACC
GGCATGCCCTATGATCTCGAAAAGGCCAAGGCACTGTTCGCGGAAGCCAAGGCGGCCGGCGTCGCCGTCGATACGCCGAT
CACGCTGGTGGCCCGCACCGACCTGTTCAGCGGCGCCGAGGAAGTCTCGCAGGCGATCCAGCAGATGATCCAGCAGGCCG
GCTTCACCGTCACGCTGAAATCGGTGGATACCGTCGGCTGGAGCCCCTGGGCTCGCAAGCCGGACTCGCTTACCCAGCCG
GTGAACCTGCTCACCTCGAGCCACAACAACATTTCGGGCGACGGTTCGCTGACCTTCCCGAACTTCCTGGGCAGCGGCGG
CCGGCTGAGCGTGGTCGACAATGCCGAACTCGATGCCAAGCTGGCGGCCGCGGCCAAGGCCAGCGGCGAAGACCGGGCCG
CGGCATATCGCGAGATCGCGCAATATGCCTATGATCAGGAACTGGTCATTCCGGTTGCGGCCCTGCAGGGGCTGCTGCTG
ACCTCGGATCGCATTGCCTACGAGGCGGATGGCTTTACCGACATCGAACTGCATCTGTCCGACGTCAAGCACAAGCAGTA
G

Upstream 100 bases:

>100_bases
TCGAACTGCATCTGTCCGAGGTGACTCTCCGTCAGTAGCGGAACCTTGCTCACTGCATCGATCCGACGCGCGTCGGGCGC
CATAGGAAGAGGAATTACGC

Downstream 100 bases:

>100_bases
TCTCGGTCCGACCGGCCGGCACAGGGTTCCAGGCACGATATGTTGAAGTTTCTCAGCCTCAGATTGACGACAAGCCTCAT
CGCGCTTGCGGGAGTGATGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MINLSKSGLAWTLLAAQAGLAFAAAPALTAEGSIVLAMPAEPTSVDACDDSTRANARVLRGNVVEALTRLDPQSGAVGPL
LATEWSSPDNKSWLFTIRPGVTFHDGTPLDAAAVAFGINRSMNPDLTCQTLSLFPTKTTATVESDMVVRITTEEPDPILP
ARIAYIDLPSPKTPEAAKSDTPIGTGPYRFAGREIGQSITLSAFDGYWGEAPEIAEANYVWRAEATIRASMIKTGEADIA
YDIPTHEAEGQANAQQYLTNGVFYLRPMLQKPPLDDLRVRQAIASSIDKATLAEVLMDNSGTPTGQLVTQLINGYVPDYT
GMPYDLEKAKALFAEAKAAGVAVDTPITLVARTDLFSGAEEVSQAIQQMIQQAGFTVTLKSVDTVGWSPWARKPDSLTQP
VNLLTSSHNNISGDGSLTFPNFLGSGGRLSVVDNAELDAKLAAAAKASGEDRAAAYREIAQYAYDQELVIPVAALQGLLL
TSDRIAYEADGFTDIELHLSDVKHKQ

Sequences:

>Translated_506_residues
MINLSKSGLAWTLLAAQAGLAFAAAPALTAEGSIVLAMPAEPTSVDACDDSTRANARVLRGNVVEALTRLDPQSGAVGPL
LATEWSSPDNKSWLFTIRPGVTFHDGTPLDAAAVAFGINRSMNPDLTCQTLSLFPTKTTATVESDMVVRITTEEPDPILP
ARIAYIDLPSPKTPEAAKSDTPIGTGPYRFAGREIGQSITLSAFDGYWGEAPEIAEANYVWRAEATIRASMIKTGEADIA
YDIPTHEAEGQANAQQYLTNGVFYLRPMLQKPPLDDLRVRQAIASSIDKATLAEVLMDNSGTPTGQLVTQLINGYVPDYT
GMPYDLEKAKALFAEAKAAGVAVDTPITLVARTDLFSGAEEVSQAIQQMIQQAGFTVTLKSVDTVGWSPWARKPDSLTQP
VNLLTSSHNNISGDGSLTFPNFLGSGGRLSVVDNAELDAKLAAAAKASGEDRAAAYREIAQYAYDQELVIPVAALQGLLL
TSDRIAYEADGFTDIELHLSDVKHKQ
>Mature_506_residues
MINLSKSGLAWTLLAAQAGLAFAAAPALTAEGSIVLAMPAEPTSVDACDDSTRANARVLRGNVVEALTRLDPQSGAVGPL
LATEWSSPDNKSWLFTIRPGVTFHDGTPLDAAAVAFGINRSMNPDLTCQTLSLFPTKTTATVESDMVVRITTEEPDPILP
ARIAYIDLPSPKTPEAAKSDTPIGTGPYRFAGREIGQSITLSAFDGYWGEAPEIAEANYVWRAEATIRASMIKTGEADIA
YDIPTHEAEGQANAQQYLTNGVFYLRPMLQKPPLDDLRVRQAIASSIDKATLAEVLMDNSGTPTGQLVTQLINGYVPDYT
GMPYDLEKAKALFAEAKAAGVAVDTPITLVARTDLFSGAEEVSQAIQQMIQQAGFTVTLKSVDTVGWSPWARKPDSLTQP
VNLLTSSHNNISGDGSLTFPNFLGSGGRLSVVDNAELDAKLAAAAKASGEDRAAAYREIAQYAYDQELVIPVAALQGLLL
TSDRIAYEADGFTDIELHLSDVKHKQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=382, Percent_Identity=27.2251308900524, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789966, Length=303, Percent_Identity=28.3828382838284, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081878, Length=508, Percent_Identity=22.6377952755905, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787762, Length=390, Percent_Identity=24.6153846153846, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787495, Length=408, Percent_Identity=23.7745098039216, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 53959; Mature: 53959

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLSKSGLAWTLLAAQAGLAFAAAPALTAEGSIVLAMPAEPTSVDACDDSTRANARVLR
CCCCCCCCCEEEEHHHHCCCHHEECCCEECCCCEEEEECCCCCCCCCCCCCCCCCCEEEH
GNVVEALTRLDPQSGAVGPLLATEWSSPDNKSWLFTIRPGVTFHDGTPLDAAAVAFGINR
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHCCC
SMNPDLTCQTLSLFPTKTTATVESDMVVRITTEEPDPILPARIAYIDLPSPKTPEAAKSD
CCCCCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCC
TPIGTGPYRFAGREIGQSITLSAFDGYWGEAPEIAEANYVWRAEATIRASMIKTGEADIA
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEECCEEEEEHEECCCCCEE
YDIPTHEAEGQANAQQYLTNGVFYLRPMLQKPPLDDLRVRQAIASSIDKATLAEVLMDNS
EECCCCCCCCCCHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GTPTGQLVTQLINGYVPDYTGMPYDLEKAKALFAEAKAAGVAVDTPITLVARTDLFSGAE
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCEEEEEECHHHCCHH
EVSQAIQQMIQQAGFTVTLKSVDTVGWSPWARKPDSLTQPVNLLTSSHNNISGDGSLTFP
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEECH
NFLGSGGRLSVVDNAELDAKLAAAAKASGEDRAAAYREIAQYAYDQELVIPVAALQGLLL
HHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCEE
TSDRIAYEADGFTDIELHLSDVKHKQ
ECCCEEEECCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MINLSKSGLAWTLLAAQAGLAFAAAPALTAEGSIVLAMPAEPTSVDACDDSTRANARVLR
CCCCCCCCCEEEEHHHHCCCHHEECCCEECCCCEEEEECCCCCCCCCCCCCCCCCCEEEH
GNVVEALTRLDPQSGAVGPLLATEWSSPDNKSWLFTIRPGVTFHDGTPLDAAAVAFGINR
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHCCC
SMNPDLTCQTLSLFPTKTTATVESDMVVRITTEEPDPILPARIAYIDLPSPKTPEAAKSD
CCCCCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCC
TPIGTGPYRFAGREIGQSITLSAFDGYWGEAPEIAEANYVWRAEATIRASMIKTGEADIA
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEECCEEEEEHEECCCCCEE
YDIPTHEAEGQANAQQYLTNGVFYLRPMLQKPPLDDLRVRQAIASSIDKATLAEVLMDNS
EECCCCCCCCCCHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GTPTGQLVTQLINGYVPDYTGMPYDLEKAKALFAEAKAAGVAVDTPITLVARTDLFSGAE
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCEEEEEECHHHCCHH
EVSQAIQQMIQQAGFTVTLKSVDTVGWSPWARKPDSLTQPVNLLTSSHNNISGDGSLTFP
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEECH
NFLGSGGRLSVVDNAELDAKLAAAAKASGEDRAAAYREIAQYAYDQELVIPVAALQGLLL
HHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCEE
TSDRIAYEADGFTDIELHLSDVKHKQ
ECCCEEEECCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA