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The map label for this gene is gsiB [H]

Identifier: 110634299

GI number: 110634299

Start: 2089268

End: 2090788

Strand: Direct

Name: gsiB [H]

Synonym: Meso_1949

Alternate gene names: 110634299

Gene position: 2089268-2090788 (Clockwise)

Preceding gene: 110634294

Following gene: 110634300

Centisome position: 47.35

GC content: 64.89

Gene sequence:

>1521_bases
ATGACCAAACTGGGGAAACGCCGCATCGTCTGGACGCTTCTGGGGGCGCAGGCCGCACTGGCTATGGCCATGGCGCCGGC
CTTCGCCGCCGAGGGCTCGATAACCCTGGCGCTGCCGTCCGAGCCGACGTCGCTCGACGCCTGCGACGACAGCACCAATG
CCAATGCCCGCGTGTTGCGCGGCAATATCGTAGAAGGGCTGACGCGGCTCGATCCGCAGACCGGGGTTGTACAGCCACTG
CTGGCGACCGAATGGACCCGCGCCGACGACAATAACTGGCTGTTTACCATCCGCCCCGGCGTCACCTTTCACGATGGCGC
CCCGCTCGATGCCGAGGCCGTGGTGTTCGGCATCAACCGTTCGATGAATCCCGACCTGGTCTGCCAGACCCTGTCGCTGT
TCGCCAACAAGACCACTGCCTCGGTCGAAAGCGAAATGGTGGTCCGCATCACCACCGAGACGCCCGATCCAATCCTGCCG
GCGCGCATCGCCTATATCGACCTGCCCTCGCCGGCCACCCCGGCAAACGCCAAGACCGACACCCCGATTGGCACCGGCCC
CTATCAGCTGGGCTCGCGCGAAATCGGCCAGTCGATTGTGCTCAATGCTTATGCGGGTTATTGGGGCGATGCACCGGCCA
TCGCCACCGCCACCTATCTCTGGCGGTCCGAGCCGACCATTCGCGCCAGCATGGTCAAAACCGGCGAAGCCGATATCGCC
ATCGACATTCCGTTCCATGAAGCCGAAGGCGCGCCCAACGCCCAGGAATATTCCACCAACAGCGTGTTCTTCCTGCGTCC
GATGCTGCGCAAGCCGCCGCTGGACGACGTGCGGGTTCGCCAGGCGGTTGCCGCCGCCATCGACAAGGTGACGCTGACGG
AAGTGCTGATGGAGCGTTCGGGCAAGCCGACCGACCAACTGGTGACCCCGCTGATCAACGGCAATGTGCCGGACTTCGCC
GGCGTGCCCTATGACGTGGAAAAGGCCAAGGCGCTGCTGGCCGAGGCCAAGGCCGATGGCGTGCCGGTGGAGACCCCGAT
CGCGCTGATCGCCCGCACCGACCTGTTCAGCGGCTCGACCGAAATCGCCCAGGCGTTGCAGCAGATGCTGCAGCAGGCGG
GCTTTACGGTGACGTTGGAAGCGGTCGACTCGGTGGCCTGGAGCCCCTGGGCGCGCAAGCCGGATTCGCTGACCCAGCCG
GTCAACCTGCTGATGTCGGCGCATGACAATATTTCGGGCGACGCATCGCTGTCCTTCCCGAACAATTTCGGCAGCGGCGG
GCGCCTCAGCATGGCCGACGATGCGGACCTGGACGCAAGGCTGGCCGCTGCGGCAGTGCTGTCGGGCGATGCCCGCACCG
CGGCCTATCGCGATATTGCCCGGGAAGCTTACGCCGAGCACATCGTTATTCCGGTCGCCGAGCTGCAAAGCCGGCTGCTG
CTGTCGGACAGGGTGAACTATCAGTCCAACGGCTTCACCGACATCGAACTGCATCTGTCCGAGGTGACTCTCCGTCAGTA
G

Upstream 100 bases:

>100_bases
GCGGAGCGCGACCCCCTATCGCCACATTCCCGCACCGCGCTTGAAACGCGGCGCTGATCTTGCTCCGGCCGGGCCGGATG
CGCATGGGAGGAGATAAAAA

Downstream 100 bases:

>100_bases
CGGAACCTTGCTCACTGCATCGATCCGACGCGCGTCGGGCGCCATAGGAAGAGGAATTACGCATGATCAACCTGTCTAAA
TCCGGCCTGGCCTGGACCTT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTKLGKRRIVWTLLGAQAALAMAMAPAFAAEGSITLALPSEPTSLDACDDSTNANARVLRGNIVEGLTRLDPQTGVVQPL
LATEWTRADDNNWLFTIRPGVTFHDGAPLDAEAVVFGINRSMNPDLVCQTLSLFANKTTASVESEMVVRITTETPDPILP
ARIAYIDLPSPATPANAKTDTPIGTGPYQLGSREIGQSIVLNAYAGYWGDAPAIATATYLWRSEPTIRASMVKTGEADIA
IDIPFHEAEGAPNAQEYSTNSVFFLRPMLRKPPLDDVRVRQAVAAAIDKVTLTEVLMERSGKPTDQLVTPLINGNVPDFA
GVPYDVEKAKALLAEAKADGVPVETPIALIARTDLFSGSTEIAQALQQMLQQAGFTVTLEAVDSVAWSPWARKPDSLTQP
VNLLMSAHDNISGDASLSFPNNFGSGGRLSMADDADLDARLAAAAVLSGDARTAAYRDIAREAYAEHIVIPVAELQSRLL
LSDRVNYQSNGFTDIELHLSEVTLRQ

Sequences:

>Translated_506_residues
MTKLGKRRIVWTLLGAQAALAMAMAPAFAAEGSITLALPSEPTSLDACDDSTNANARVLRGNIVEGLTRLDPQTGVVQPL
LATEWTRADDNNWLFTIRPGVTFHDGAPLDAEAVVFGINRSMNPDLVCQTLSLFANKTTASVESEMVVRITTETPDPILP
ARIAYIDLPSPATPANAKTDTPIGTGPYQLGSREIGQSIVLNAYAGYWGDAPAIATATYLWRSEPTIRASMVKTGEADIA
IDIPFHEAEGAPNAQEYSTNSVFFLRPMLRKPPLDDVRVRQAVAAAIDKVTLTEVLMERSGKPTDQLVTPLINGNVPDFA
GVPYDVEKAKALLAEAKADGVPVETPIALIARTDLFSGSTEIAQALQQMLQQAGFTVTLEAVDSVAWSPWARKPDSLTQP
VNLLMSAHDNISGDASLSFPNNFGSGGRLSMADDADLDARLAAAAVLSGDARTAAYRDIAREAYAEHIVIPVAELQSRLL
LSDRVNYQSNGFTDIELHLSEVTLRQ
>Mature_505_residues
TKLGKRRIVWTLLGAQAALAMAMAPAFAAEGSITLALPSEPTSLDACDDSTNANARVLRGNIVEGLTRLDPQTGVVQPLL
ATEWTRADDNNWLFTIRPGVTFHDGAPLDAEAVVFGINRSMNPDLVCQTLSLFANKTTASVESEMVVRITTETPDPILPA
RIAYIDLPSPATPANAKTDTPIGTGPYQLGSREIGQSIVLNAYAGYWGDAPAIATATYLWRSEPTIRASMVKTGEADIAI
DIPFHEAEGAPNAQEYSTNSVFFLRPMLRKPPLDDVRVRQAVAAAIDKVTLTEVLMERSGKPTDQLVTPLINGNVPDFAG
VPYDVEKAKALLAEAKADGVPVETPIALIARTDLFSGSTEIAQALQQMLQQAGFTVTLEAVDSVAWSPWARKPDSLTQPV
NLLMSAHDNISGDASLSFPNNFGSGGRLSMADDADLDARLAAAAVLSGDARTAAYRDIAREAYAEHIVIPVAELQSRLLL
SDRVNYQSNGFTDIELHLSEVTLRQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=381, Percent_Identity=27.2965879265092, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789966, Length=370, Percent_Identity=28.3783783783784, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789887, Length=503, Percent_Identity=24.8508946322068, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787762, Length=400, Percent_Identity=24.75, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54263; Mature: 54132

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLGKRRIVWTLLGAQAALAMAMAPAFAAEGSITLALPSEPTSLDACDDSTNANARVLR
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEE
GNIVEGLTRLDPQTGVVQPLLATEWTRADDNNWLFTIRPGVTFHDGAPLDAEAVVFGINR
CHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCCCEEEEECCC
SMNPDLVCQTLSLFANKTTASVESEMVVRITTETPDPILPARIAYIDLPSPATPANAKTD
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC
TPIGTGPYQLGSREIGQSIVLNAYAGYWGDAPAIATATYLWRSEPTIRASMVKTGEADIA
CCCCCCCHHCCCHHHCCCEEEEECCCCCCCCCHHHHHHHEECCCCCEEEEEEECCCCCEE
IDIPFHEAEGAPNAQEYSTNSVFFLRPMLRKPPLDDVRVRQAVAAAIDKVTLTEVLMERS
EEECCHHCCCCCCHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GKPTDQLVTPLINGNVPDFAGVPYDVEKAKALLAEAKADGVPVETPIALIARTDLFSGST
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCH
EIAQALQQMLQQAGFTVTLEAVDSVAWSPWARKPDSLTQPVNLLMSAHDNISGDASLSFP
HHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECC
NNFGSGGRLSMADDADLDARLAAAAVLSGDARTAAYRDIAREAYAEHIVIPVAELQSRLL
CCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHH
LSDRVNYQSNGFTDIELHLSEVTLRQ
HHHCCCCCCCCCEEEEEEEEEHEECC
>Mature Secondary Structure 
TKLGKRRIVWTLLGAQAALAMAMAPAFAAEGSITLALPSEPTSLDACDDSTNANARVLR
CCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEE
GNIVEGLTRLDPQTGVVQPLLATEWTRADDNNWLFTIRPGVTFHDGAPLDAEAVVFGINR
CHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCCCEEEEECCC
SMNPDLVCQTLSLFANKTTASVESEMVVRITTETPDPILPARIAYIDLPSPATPANAKTD
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC
TPIGTGPYQLGSREIGQSIVLNAYAGYWGDAPAIATATYLWRSEPTIRASMVKTGEADIA
CCCCCCCHHCCCHHHCCCEEEEECCCCCCCCCHHHHHHHEECCCCCEEEEEEECCCCCEE
IDIPFHEAEGAPNAQEYSTNSVFFLRPMLRKPPLDDVRVRQAVAAAIDKVTLTEVLMERS
EEECCHHCCCCCCHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GKPTDQLVTPLINGNVPDFAGVPYDVEKAKALLAEAKADGVPVETPIALIARTDLFSGST
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCH
EIAQALQQMLQQAGFTVTLEAVDSVAWSPWARKPDSLTQPVNLLMSAHDNISGDASLSFP
HHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECC
NNFGSGGRLSMADDADLDARLAAAAVLSGDARTAAYRDIAREAYAEHIVIPVAELQSRLL
CCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHH
LSDRVNYQSNGFTDIELHLSEVTLRQ
HHHCCCCCCCCCEEEEEEEEEHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA