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The map label for this gene is appB [H]

Identifier: 110634301

GI number: 110634301

Start: 2092411

End: 2093328

Strand: Direct

Name: appB [H]

Synonym: Meso_1951

Alternate gene names: 110634301

Gene position: 2092411-2093328 (Clockwise)

Preceding gene: 110634300

Following gene: 110634302

Centisome position: 47.42

GC content: 63.94

Gene sequence:

>918_bases
ATGTTGAAGTTTCTCAGCCTCAGATTGACGACAAGCCTCATCGCGCTTGCGGGAGTGATGGTCATTGTCTTCTTCTTGGC
CCGGCTAACGGGAAGTCCGGCGACGCTCTACCTGCCGGCGGACGCCTCCGTCGAAATGATGGAGGCCTTCAACCGGCAGT
TTGGCTACGACCAGCCGATCCCGCAGCAGTTCGCGCAATTCGTGGGCAATGTGTTCCGGCTCGACTTTGGCCAGTCGTTG
TATCAGCAACGGCCGGCTGCCGTGGCGGTCCTATCGGCCATGCCGCAGACCATCCTGCTGGCTGCGGTGGCCATGGCGCT
GACCCTGCTCGCCGCCATTCCCCTCGGAACCGTCGCGGCCCTGCACCCCCATTCGGTGCAGGACCGGCTGATCACGCTAT
TCTCGCTGCTGACCGCCTCGATCCCCGATTTCTGGTTCGCGCTGGTCGGCGTCCTGGTGCTGTCGGTCAATCTGGGATTG
TTGCCGACTTCCGGGCAGGCGAGCCTGCTCTCCTGGATCCTTCCGGTGGCGACGCTGATGCTGCATCCGGTAGGCGTGCT
GACCCAGGTGGTGCGCGGCGCCATGCTGGAAACGCTCAATGCCGGATATGTGCAAAATGCCCGGGCTCGCGGCTATTCGT
GGAAGCGGCTGGCCTTCCGCCATGCGCTGCGCAACGCCGCGCTGCCGATCATCACCGTGGCCGGCGACCGGGCCGGCGGC
ATGGTCAATGGCGCGGTCATCGTTTCGGCGGTGTTTGCCTGGCCCGGCATCGGCCAGGTGTTGGTCGACGCTGTCTATAA
CCGCGACTTCGCCGTGATCCAGGCCGGGGTCTTCGTCGTCGGCATTGCCGTCATCCTGCTAAATTTCCTCGTGGACCTGA
TCTACGCGGCGGCCGACCCAAGGATCCGGATTTCCTGA

Upstream 100 bases:

>100_bases
CGAGGCGGATGGCTTTACCGACATCGAACTGCATCTGTCCGACGTCAAGCACAAGCAGTAGTCTCGGTCCGACCGGCCGG
CACAGGGTTCCAGGCACGAT

Downstream 100 bases:

>100_bases
CATGTATCTGACCCAACCCATTCCTGCATCGCCGGCCTCGACCGGCGCGCGCATCAAGGCCTATTTCGCCCAGACACGGC
TCAGCCTTCTGGTTCGTTCG

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLKFLSLRLTTSLIALAGVMVIVFFLARLTGSPATLYLPADASVEMMEAFNRQFGYDQPIPQQFAQFVGNVFRLDFGQSL
YQQRPAAVAVLSAMPQTILLAAVAMALTLLAAIPLGTVAALHPHSVQDRLITLFSLLTASIPDFWFALVGVLVLSVNLGL
LPTSGQASLLSWILPVATLMLHPVGVLTQVVRGAMLETLNAGYVQNARARGYSWKRLAFRHALRNAALPIITVAGDRAGG
MVNGAVIVSAVFAWPGIGQVLVDAVYNRDFAVIQAGVFVVGIAVILLNFLVDLIYAAADPRIRIS

Sequences:

>Translated_305_residues
MLKFLSLRLTTSLIALAGVMVIVFFLARLTGSPATLYLPADASVEMMEAFNRQFGYDQPIPQQFAQFVGNVFRLDFGQSL
YQQRPAAVAVLSAMPQTILLAAVAMALTLLAAIPLGTVAALHPHSVQDRLITLFSLLTASIPDFWFALVGVLVLSVNLGL
LPTSGQASLLSWILPVATLMLHPVGVLTQVVRGAMLETLNAGYVQNARARGYSWKRLAFRHALRNAALPIITVAGDRAGG
MVNGAVIVSAVFAWPGIGQVLVDAVYNRDFAVIQAGVFVVGIAVILLNFLVDLIYAAADPRIRIS
>Mature_305_residues
MLKFLSLRLTTSLIALAGVMVIVFFLARLTGSPATLYLPADASVEMMEAFNRQFGYDQPIPQQFAQFVGNVFRLDFGQSL
YQQRPAAVAVLSAMPQTILLAAVAMALTLLAAIPLGTVAALHPHSVQDRLITLFSLLTASIPDFWFALVGVLVLSVNLGL
LPTSGQASLLSWILPVATLMLHPVGVLTQVVRGAMLETLNAGYVQNARARGYSWKRLAFRHALRNAALPIITVAGDRAGG
MVNGAVIVSAVFAWPGIGQVLVDAVYNRDFAVIQAGVFVVGIAVILLNFLVDLIYAAADPRIRIS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=307, Percent_Identity=35.5048859934853, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787053, Length=304, Percent_Identity=35.1973684210526, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789965, Length=336, Percent_Identity=30.952380952381, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787497, Length=309, Percent_Identity=31.0679611650485, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787550, Length=257, Percent_Identity=34.6303501945525, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787761, Length=330, Percent_Identity=32.7272727272727, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788504, Length=278, Percent_Identity=25.8992805755396, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32682; Mature: 32682

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFLSLRLTTSLIALAGVMVIVFFLARLTGSPATLYLPADASVEMMEAFNRQFGYDQPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCC
PQQFAQFVGNVFRLDFGQSLYQQRPAAVAVLSAMPQTILLAAVAMALTLLAAIPLGTVAA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHPHSVQDRLITLFSLLTASIPDFWFALVGVLVLSVNLGLLPTSGQASLLSWILPVATLM
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LHPVGVLTQVVRGAMLETLNAGYVQNARARGYSWKRLAFRHALRNAALPIITVAGDRAGG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
MVNGAVIVSAVFAWPGIGQVLVDAVYNRDFAVIQAGVFVVGIAVILLNFLVDLIYAAADP
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIRIS
CEECC
>Mature Secondary Structure
MLKFLSLRLTTSLIALAGVMVIVFFLARLTGSPATLYLPADASVEMMEAFNRQFGYDQPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCC
PQQFAQFVGNVFRLDFGQSLYQQRPAAVAVLSAMPQTILLAAVAMALTLLAAIPLGTVAA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHPHSVQDRLITLFSLLTASIPDFWFALVGVLVLSVNLGLLPTSGQASLLSWILPVATLM
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LHPVGVLTQVVRGAMLETLNAGYVQNARARGYSWKRLAFRHALRNAALPIITVAGDRAGG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
MVNGAVIVSAVFAWPGIGQVLVDAVYNRDFAVIQAGVFVVGIAVILLNFLVDLIYAAADP
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIRIS
CEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]