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The map label for this gene is lipB

Identifier: 110633399

GI number: 110633399

Start: 1147890

End: 1148630

Strand: Reverse

Name: lipB

Synonym: Meso_1045

Alternate gene names: 110633399

Gene position: 1148630-1147890 (Counterclockwise)

Preceding gene: 110633400

Following gene: 110633398

Centisome position: 26.03

GC content: 64.1

Gene sequence:

>741_bases
ATGAGCCAGACAAGCCAAACCCGCTTCATCCCGCATTCCCCCTCGCCACCGGTTGAATGGCGAATCGAACCGGGCCTTAC
TCCCTACGAAAAGGCGCTTGCCTTCATGGAACAGCGCGCAGACGCCATCCGCGCCGGCACGGCGGGTGAGATGGTGTGGC
TGGTCGAGCACCCTCCCCTTTACACAGCCGGCACGAGCGCCAGCCCGGGCGACCTGCTCGATCCGAATCGCCTGCCCGTC
TACCAGACCGGCCGCGGCGGCGAATACACTTATCATGGTCCGGGTCAGCGCGTGGCCTATGTCATGCTCGACCTGAAACG
CCGGCGCGAGGACGTGCGCGCCTTCGTCACCGCGCTGGAGGATTGGATCATCGCCAGCCTCGCCCGTTTCAACATCCGTG
GCGAGCGCCGGCAGGGACGCGTCGGGGTGTGGGTGGCGCGCCCGGAAAAATCCCCGCTGCCGGACGGAACGATCATGGAA
GACAAGATCGCAGCCATCGGCATCCGTCTACGGCGCTGGGTGAGCTTTCACGGCATTGCCGTCAATGTGGAGCCGGAGCT
TGCGCATTTTTCGGGCATCGTGCCCTGCGGAATCTCGGGCTTCGGCGTCACCAGCCTCGTCGATCTCGGCCTGCCCGTCA
CCATGGCCGATTTCGACGTTGCGCTAAAGGAAGCGTTCGAGGAGGTCTTCGGGGCAGCAGAGCCGCTTTTCGAAACGGAT
TCCCTGAAAAGGAGTGCCTGA

Upstream 100 bases:

>100_bases
GAATGCAAATATGCAAAAAGAAAACAAAGCCGCAGCGCGCGCCCCCGCCCTCTCGACTTCGCGCCGTCGCAGGGCCATAG
AAAGTGCGGAGCATCAGCCC

Downstream 100 bases:

>100_bases
CATGGGTGTAAGAGAAACACTCGCCAAGGGCGCAATGGCTCTCGGCCTTCTCCTTGCCACACCCGCCCTGGCCCAGCAGG
CGGCCGACACGCTTGCTCCC

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSQTSQTRFIPHSPSPPVEWRIEPGLTPYEKALAFMEQRADAIRAGTAGEMVWLVEHPPLYTAGTSASPGDLLDPNRLPV
YQTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVTALEDWIIASLARFNIRGERRQGRVGVWVARPEKSPLPDGTIME
DKIAAIGIRLRRWVSFHGIAVNVEPELAHFSGIVPCGISGFGVTSLVDLGLPVTMADFDVALKEAFEEVFGAAEPLFETD
SLKRSA

Sequences:

>Translated_246_residues
MSQTSQTRFIPHSPSPPVEWRIEPGLTPYEKALAFMEQRADAIRAGTAGEMVWLVEHPPLYTAGTSASPGDLLDPNRLPV
YQTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVTALEDWIIASLARFNIRGERRQGRVGVWVARPEKSPLPDGTIME
DKIAAIGIRLRRWVSFHGIAVNVEPELAHFSGIVPCGISGFGVTSLVDLGLPVTMADFDVALKEAFEEVFGAAEPLFETD
SLKRSA
>Mature_245_residues
SQTSQTRFIPHSPSPPVEWRIEPGLTPYEKALAFMEQRADAIRAGTAGEMVWLVEHPPLYTAGTSASPGDLLDPNRLPVY
QTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVTALEDWIIASLARFNIRGERRQGRVGVWVARPEKSPLPDGTIMED
KIAAIGIRLRRWVSFHGIAVNVEPELAHFSGIVPCGISGFGVTSLVDLGLPVTMADFDVALKEAFEEVFGAAEPLFETDS
LKRSA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=232, Percent_Identity=32.7586206896552, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI87081767, Length=182, Percent_Identity=40.6593406593407, Blast_Score=138, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323268, Length=195, Percent_Identity=25.6410256410256, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355483, Length=185, Percent_Identity=36.7567567567568, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_MESSB (Q11JI3)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673607.1
- ProteinModelPortal:   Q11JI3
- SMR:   Q11JI3
- STRING:   Q11JI3
- GeneID:   4180364
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1045
- NMPDR:   fig|266779.1.peg.2536
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q11JI3
- BioCyc:   MSP266779:MESO_1045-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 27174; Mature: 27043

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSQTRFIPHSPSPPVEWRIEPGLTPYEKALAFMEQRADAIRAGTAGEMVWLVEHPPL
CCCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCE
YTAGTSASPGDLLDPNRLPVYQTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVTALE
EECCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHH
DWIIASLARFNIRGERRQGRVGVWVARPEKSPLPDGTIMEDKIAAIGIRLRRWVSFHGIA
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCEE
VNVEPELAHFSGIVPCGISGFGVTSLVDLGLPVTMADFDVALKEAFEEVFGAAEPLFETD
EEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCC
SLKRSA
CCCCCC
>Mature Secondary Structure 
SQTSQTRFIPHSPSPPVEWRIEPGLTPYEKALAFMEQRADAIRAGTAGEMVWLVEHPPL
CCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCE
YTAGTSASPGDLLDPNRLPVYQTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVTALE
EECCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHH
DWIIASLARFNIRGERRQGRVGVWVARPEKSPLPDGTIMEDKIAAIGIRLRRWVSFHGIA
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCEE
VNVEPELAHFSGIVPCGISGFGVTSLVDLGLPVTMADFDVALKEAFEEVFGAAEPLFETD
EEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCC
SLKRSA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA