Click here to switch to the map view.

The map label for this gene is pgtE [H]

Identifier: 110632419

GI number: 110632419

Start: 79083

End: 80045

Strand: Direct

Name: pgtE [H]

Synonym: Meso_0057

Alternate gene names: 110632419

Gene position: 79083-80045 (Clockwise)

Preceding gene: 110632418

Following gene: 110632420

Centisome position: 1.79

GC content: 57.11

Gene sequence:

>963_bases
ATGAGAAAGCATTTAATGCTTCACACGGGCATCTTGGGCTTGTGCATCCTCCCGTTCATCGGAACTGCGCATGCTGCGGA
CCCTGTGACTTTTTCGGGCTTGTTTCTCGCGCAGAACGAACAGTTCCGTTTCTCTGTTGAGGCTGGCTACCTCACAGCCA
GGGCGAACGAATACGTCTATGAGGAAGGCAGAACAGTAAGCAGGCTGATATGGGACATCAATGCCGCGGCAACCATAACT
GCCCGCGCGGAAATGAACATGACACCGCGATGGCGGCTGTCGGGCTCCGCAACGGTCGGGATCGACCACAGCAATTTCAT
GGCCGATTATGACTGGTTGACGCCAAGTCCTGACTGGTCCCACCGTTCCGTCCATTGGGACACGCAGCTTGACCATTATG
TGGCGCTGGACCTGGGGGCCAGCTATGCCGCCTGGCAGATCAATAGGACGACCCTGAACATTCTCGGCGGGCTGCGATAT
ACTGATTTTCAGTTAAGCTCCTACGGCGGCTCCTTCGTTTATACGACCGAAGACGGTGAGTTTCGCGGGGATAGTTTGAG
TTTTCCTGACGGCCAGGCCGTAATCACCTATCGCCAGCGGCTGCCGGCGCTCTATGGAGGGTTAGGCGCATCCCGCCAGA
TCGGCAGCGTGCATTTCGATGCAGGCATACTGGGCGGCGTGACCCTGTGGCCGCGCGATAAGGATCACCACTGGCTGCGG
TCGCTCAAATTCAAGGACGAGTTCAAGCGAAGTCCTTATCTGGCCCTGCAGCTGCGGGCGAGCGTGCCTGTTTCCGACAA
GGGCGAGGCATTCCTGTCGGCACGCTTCGAAAAATATTTCCGCATGGAAGGCAACACGCACATTTTGGACACGCGCACCG
GCCAAAGCTCTCTCTTTCAAGATAGCGCAGGCGCTTCGCTGCAGGCGTTGAATATAGGCGTCGGCCTGCGCTTCAGGTTT
TAG

Upstream 100 bases:

>100_bases
ATTTGCGCATAAAACAGGCGGATCACTAGAACCGGCATATCGCGCTGACGGGTTCCTGAAGTTCCGGATCTGCCGCACAG
CATGGATCGAAGGAGGATTC

Downstream 100 bases:

>100_bases
CGCGTTCCCTGCCGCGTGCTCTCTCCGACTTAGAGGTTATGGACTCCTGCAACTTGTGAGGTAAGCTCGGCGCTATTACG
CGCCTTTGAGTGCCGGTCGC

Product: outer membrane protease

Products: Hydrolyzed protein [C]

Alternate protein names: E protein [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRKHLMLHTGILGLCILPFIGTAHAADPVTFSGLFLAQNEQFRFSVEAGYLTARANEYVYEEGRTVSRLIWDINAAATIT
ARAEMNMTPRWRLSGSATVGIDHSNFMADYDWLTPSPDWSHRSVHWDTQLDHYVALDLGASYAAWQINRTTLNILGGLRY
TDFQLSSYGGSFVYTTEDGEFRGDSLSFPDGQAVITYRQRLPALYGGLGASRQIGSVHFDAGILGGVTLWPRDKDHHWLR
SLKFKDEFKRSPYLALQLRASVPVSDKGEAFLSARFEKYFRMEGNTHILDTRTGQSSLFQDSAGASLQALNIGVGLRFRF

Sequences:

>Translated_320_residues
MRKHLMLHTGILGLCILPFIGTAHAADPVTFSGLFLAQNEQFRFSVEAGYLTARANEYVYEEGRTVSRLIWDINAAATIT
ARAEMNMTPRWRLSGSATVGIDHSNFMADYDWLTPSPDWSHRSVHWDTQLDHYVALDLGASYAAWQINRTTLNILGGLRY
TDFQLSSYGGSFVYTTEDGEFRGDSLSFPDGQAVITYRQRLPALYGGLGASRQIGSVHFDAGILGGVTLWPRDKDHHWLR
SLKFKDEFKRSPYLALQLRASVPVSDKGEAFLSARFEKYFRMEGNTHILDTRTGQSSLFQDSAGASLQALNIGVGLRFRF
>Mature_320_residues
MRKHLMLHTGILGLCILPFIGTAHAADPVTFSGLFLAQNEQFRFSVEAGYLTARANEYVYEEGRTVSRLIWDINAAATIT
ARAEMNMTPRWRLSGSATVGIDHSNFMADYDWLTPSPDWSHRSVHWDTQLDHYVALDLGASYAAWQINRTTLNILGGLRY
TDFQLSSYGGSFVYTTEDGEFRGDSLSFPDGQAVITYRQRLPALYGGLGASRQIGSVHFDAGILGGVTLWPRDKDHHWLR
SLKFKDEFKRSPYLALQLRASVPVSDKGEAFLSARFEKYFRMEGNTHILDTRTGQSSLFQDSAGASLQALNIGVGLRFRF

Specific function: Protease that can cleave T7 RNA polymerase. Specific for paired basic residues [H]

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family [H]

Homologues:

Organism=Escherichia coli, GI1786777, Length=330, Percent_Identity=26.969696969697, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079 [H]

Pfam domain/function: PF01278 Omptin [H]

EC number: 3.4.21.87 [C]

Molecular weight: Translated: 35893; Mature: 35893

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKHLMLHTGILGLCILPFIGTAHAADPVTFSGLFLAQNEQFRFSVEAGYLTARANEYVY
CCCEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCEEEEEECCEEEECCCCEEE
EEGRTVSRLIWDINAAATITARAEMNMTPRWRLSGSATVGIDHSNFMADYDWLTPSPDWS
CCCCEEEEEEEECCCEEEEEEEEECCCCCCEEECCCEEEEECCCCCEEECCCCCCCCCCC
HRSVHWDTQLDHYVALDLGASYAAWQINRTTLNILGGLRYTDFQLSSYGGSFVYTTEDGE
CCEEEECCCCCEEEEEECCCCEEEEEEECEEEEECCCCEEEEEEEECCCCEEEEECCCCC
FRGDSLSFPDGQAVITYRQRLPALYGGLGASRQIGSVHFDAGILGGVTLWPRDKDHHWLR
CCCCCCCCCCCCEEEEEHHHCHHHHCCCCCCCCCCEEEECCEEECCEEECCCCCCCHHHH
SLKFKDEFKRSPYLALQLRASVPVSDKGEAFLSARFEKYFRMEGNTHILDTRTGQSSLFQ
HCCCHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCEEEEECCCCCHHHHC
DSAGASLQALNIGVGLRFRF
CCCCCEEEEEEEEEEEEEEC
>Mature Secondary Structure
MRKHLMLHTGILGLCILPFIGTAHAADPVTFSGLFLAQNEQFRFSVEAGYLTARANEYVY
CCCEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCEEEEEECCEEEECCCCEEE
EEGRTVSRLIWDINAAATITARAEMNMTPRWRLSGSATVGIDHSNFMADYDWLTPSPDWS
CCCCEEEEEEEECCCEEEEEEEEECCCCCCEEECCCEEEEECCCCCEEECCCCCCCCCCC
HRSVHWDTQLDHYVALDLGASYAAWQINRTTLNILGGLRYTDFQLSSYGGSFVYTTEDGE
CCEEEECCCCCEEEEEECCCCEEEEEEECEEEEECCCCEEEEEEEECCCCEEEEECCCCC
FRGDSLSFPDGQAVITYRQRLPALYGGLGASRQIGSVHFDAGILGGVTLWPRDKDHHWLR
CCCCCCCCCCCCEEEEEHHHCHHHHCCCCCCCCCCEEEECCEEECCEEECCCCCCCHHHH
SLKFKDEFKRSPYLALQLRASVPVSDKGEAFLSARFEKYFRMEGNTHILDTRTGQSSLFQ
HCCCHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCEEEEECCCCCHHHHC
DSAGASLQALNIGVGLRFRF
CCCCCEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: EDTA; Tosyl-L-Lys chloromethyl ketone [C]

Metal ions: NA

Kcat value (1/min): 40 [C]

Specific activity: 58.7

Km value (mM): 0.0036 {plasminogen}} 0.000058 {plasminogen}} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Arginine; Benzamidine; Co2+; Cu2+; Diisopropyl fluorophosphate; Fe2+; NaCl; Tosyl -L-phenyl alaninechloromethyl ketone; Zn2+ [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3023201; 10869088; 11677609; 2651422 [H]