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The map label for this gene is fumA [H]

Identifier: 110632418

GI number: 110632418

Start: 76758

End: 78266

Strand: Direct

Name: fumA [H]

Synonym: Meso_0056

Alternate gene names: 110632418

Gene position: 76758-78266 (Clockwise)

Preceding gene: 110632417

Following gene: 110632419

Centisome position: 1.74

GC content: 64.21

Gene sequence:

>1509_bases
ATGACCCGTATTTCCAACGCAGCGATCGTCACTAACATCGCCGCCGCGCTGCAGTACATCTCGGTCTTCCATCCGCCCGA
GTACATCCGCCACCTTGCGGCTGCCCACGCACAAGAGGAGAGCTTGCCCGCCCGCGCCGCAATCGAGCAGATCCTGATCA
GTTCGCGCATGGCCGCGCTCGGGCAACGTGCCATTTGTCAGGACACCGGCACTGTCAACGCGCATATGAAAGTGGGCATG
GGGGTGCGGCTTGATGGTGATTCCAGCCTTCAGAACCTGCTCGACGAAGCGATCCGGCTAGCCTGGGCCGATGACCGCAA
CCCGCTGAGGGCCTCCATCGTCGCCGACCCGCTGTTCGCGCGCCGCAATACCCGCGACAACACCCCGGGCATGCTGACCG
TGGAAATCGTGCCCGGAGACGGTATCGAAATTGCCCTCATGTCGAAGGGCGGCGGCTCGGAGAACAAGAGCCGCTTCACC
ACACTCAGGCCTTCAGACAGCGTCGAGGACTGGGTAATCGATCAGCTTCCCGGCTTGGGCGCCGGCTGGTGCCCTCCGGG
CATGCTGGGCATCGGGGTCGGCGGCAGCGCCGACATGGCCATGCGGCTTGCCAAGGAGAGCCTTTACGATGCGCCCAACA
TGGCATGGTTACGACAGAAGGCGAACCACACCGAAGAGGAGGCCTTCCGCGTCCGCGTCTGTGACCGGGCCAACGCACTC
GGCATTGGTGCCCAGGGGTTGGGTGGGCTGACCGCGGTGCTCGACGTAAAGCTACGGACCGCGCCAACGCATGCCTCTGC
GCTGCCGGTCGCGCTGATCCCGCAATGCGTGGCAACGCGAATGGTCCGCTTCCGCATCTCGGCAACCGAGGGCTTCGATC
TCTCGCCGCCCGATCCTGCGCTTTGGCCGGAGATCACCGCCCCCGCCGTTGACGGAGTCCAGCGGATCAATCTCGACACC
TTGACCGAGTCCGCGAAGCAGAGTTGGAAAGCAGGCGACACCCTGCTTCTGTCGGGCCGCATCCTGACTGGCCGTGATGC
AGCCCACAAGCGCCTCGACGAAATGCTGGATCGCGGCGATCCCCTGCCCGTCGATTTGCGCGGCCGCGCGATCTATTATG
TCGGTCCGGTCGATCCCGCTCCGGGCGAGATCATCGGCCCGGCGGGACCCACCACTTCCACCCGAATGGACAAATTCACT
CCCCGTCTTCTTGCCCAAACCGGTCTCGGACTGATGATTGGGAAGGCCGAGCGTGGACCAGACGTTATTGAAGCGATCGC
TCGCCACAAGGCCCCGTACCTCATCGCCGTCGGCGGCGCAGCCGTTCTCATCTCCCGCGCGATCCGCAACGCCCGCGTCG
TCGCCTTCGAGGACCTCGGCATGGAAGCCATCCACGAATTCGAAGTCCAGGACCTGCCCGTCATCGTCGCAGTCGACACC
GCGGGTACATCGATACACGACACAGGACCCGCGCAGTGGCGTAGAACGTCAACCAGCCGGTCTTCTTAA

Upstream 100 bases:

>100_bases
TGAATAACTATCCGACACTCGCCCGCGCCCATGCGGCGATGACGGCCCTGCCGGAGGTGCAGGCTGCCCATCCGGACCGC
CAGCCTGACGCAGCCAAAGC

Downstream 100 bases:

>100_bases
CCGATAAGTGTGCGAGAAGCTGTCGCCAGCTTTGTGTGCCCAAAGCTGCATGGCAGCGGTTGCGTTGAAAGCGGGCATGC
TGATCCGTGCTACGCCGCCC

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTRISNAAIVTNIAAALQYISVFHPPEYIRHLAAAHAQEESLPARAAIEQILISSRMAALGQRAICQDTGTVNAHMKVGM
GVRLDGDSSLQNLLDEAIRLAWADDRNPLRASIVADPLFARRNTRDNTPGMLTVEIVPGDGIEIALMSKGGGSENKSRFT
TLRPSDSVEDWVIDQLPGLGAGWCPPGMLGIGVGGSADMAMRLAKESLYDAPNMAWLRQKANHTEEEAFRVRVCDRANAL
GIGAQGLGGLTAVLDVKLRTAPTHASALPVALIPQCVATRMVRFRISATEGFDLSPPDPALWPEITAPAVDGVQRINLDT
LTESAKQSWKAGDTLLLSGRILTGRDAAHKRLDEMLDRGDPLPVDLRGRAIYYVGPVDPAPGEIIGPAGPTTSTRMDKFT
PRLLAQTGLGLMIGKAERGPDVIEAIARHKAPYLIAVGGAAVLISRAIRNARVVAFEDLGMEAIHEFEVQDLPVIVAVDT
AGTSIHDTGPAQWRRTSTSRSS

Sequences:

>Translated_502_residues
MTRISNAAIVTNIAAALQYISVFHPPEYIRHLAAAHAQEESLPARAAIEQILISSRMAALGQRAICQDTGTVNAHMKVGM
GVRLDGDSSLQNLLDEAIRLAWADDRNPLRASIVADPLFARRNTRDNTPGMLTVEIVPGDGIEIALMSKGGGSENKSRFT
TLRPSDSVEDWVIDQLPGLGAGWCPPGMLGIGVGGSADMAMRLAKESLYDAPNMAWLRQKANHTEEEAFRVRVCDRANAL
GIGAQGLGGLTAVLDVKLRTAPTHASALPVALIPQCVATRMVRFRISATEGFDLSPPDPALWPEITAPAVDGVQRINLDT
LTESAKQSWKAGDTLLLSGRILTGRDAAHKRLDEMLDRGDPLPVDLRGRAIYYVGPVDPAPGEIIGPAGPTTSTRMDKFT
PRLLAQTGLGLMIGKAERGPDVIEAIARHKAPYLIAVGGAAVLISRAIRNARVVAFEDLGMEAIHEFEVQDLPVIVAVDT
AGTSIHDTGPAQWRRTSTSRSS
>Mature_501_residues
TRISNAAIVTNIAAALQYISVFHPPEYIRHLAAAHAQEESLPARAAIEQILISSRMAALGQRAICQDTGTVNAHMKVGMG
VRLDGDSSLQNLLDEAIRLAWADDRNPLRASIVADPLFARRNTRDNTPGMLTVEIVPGDGIEIALMSKGGGSENKSRFTT
LRPSDSVEDWVIDQLPGLGAGWCPPGMLGIGVGGSADMAMRLAKESLYDAPNMAWLRQKANHTEEEAFRVRVCDRANALG
IGAQGLGGLTAVLDVKLRTAPTHASALPVALIPQCVATRMVRFRISATEGFDLSPPDPALWPEITAPAVDGVQRINLDTL
TESAKQSWKAGDTLLLSGRILTGRDAAHKRLDEMLDRGDPLPVDLRGRAIYYVGPVDPAPGEIIGPAGPTTSTRMDKFTP
RLLAQTGLGLMIGKAERGPDVIEAIARHKAPYLIAVGGAAVLISRAIRNARVVAFEDLGMEAIHEFEVQDLPVIVAVDTA
GTSIHDTGPAQWRRTSTSRSS

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=482, Percent_Identity=29.4605809128631, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1787897, Length=460, Percent_Identity=31.304347826087, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1789443, Length=164, Percent_Identity=35.9756097560976, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789442, Length=292, Percent_Identity=28.0821917808219, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 53697; Mature: 53566

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRISNAAIVTNIAAALQYISVFHPPEYIRHLAAAHAQEESLPARAAIEQILISSRMAAL
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GQRAICQDTGTVNAHMKVGMGVRLDGDSSLQNLLDEAIRLAWADDRNPLRASIVADPLFA
CCHHHHCCCCCEEEEEEECEEEEECCCHHHHHHHHHHHHEEECCCCCCCEEHHHHCHHHH
RRNTRDNTPGMLTVEIVPGDGIEIALMSKGGGSENKSRFTTLRPSDSVEDWVIDQLPGLG
HCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCC
AGWCPPGMLGIGVGGSADMAMRLAKESLYDAPNMAWLRQKANHTEEEAFRVRVCDRANAL
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHEEEEECCCCCC
GIGAQGLGGLTAVLDVKLRTAPTHASALPVALIPQCVATRMVRFRISATEGFDLSPPDPA
CCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
LWPEITAPAVDGVQRINLDTLTESAKQSWKAGDTLLLSGRILTGRDAAHKRLDEMLDRGD
CCCCCCCHHHCCHHHCCHHHHHHHHHHHHCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
PLPVDLRGRAIYYVGPVDPAPGEIIGPAGPTTSTRMDKFTPRLLAQTGLGLMIGKAERGP
CCCEEECCCEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCH
DVIEAIARHKAPYLIAVGGAAVLISRAIRNARVVAFEDLGMEAIHEFEVQDLPVIVAVDT
HHHHHHHHCCCCEEEEECHHHHHHHHHHHCCEEEEEHHCCHHHHHHCCCCCCCEEEEEEC
AGTSIHDTGPAQWRRTSTSRSS
CCCCCCCCCCHHHEECCCCCCC
>Mature Secondary Structure 
TRISNAAIVTNIAAALQYISVFHPPEYIRHLAAAHAQEESLPARAAIEQILISSRMAAL
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GQRAICQDTGTVNAHMKVGMGVRLDGDSSLQNLLDEAIRLAWADDRNPLRASIVADPLFA
CCHHHHCCCCCEEEEEEECEEEEECCCHHHHHHHHHHHHEEECCCCCCCEEHHHHCHHHH
RRNTRDNTPGMLTVEIVPGDGIEIALMSKGGGSENKSRFTTLRPSDSVEDWVIDQLPGLG
HCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCC
AGWCPPGMLGIGVGGSADMAMRLAKESLYDAPNMAWLRQKANHTEEEAFRVRVCDRANAL
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHEEEEECCCCCC
GIGAQGLGGLTAVLDVKLRTAPTHASALPVALIPQCVATRMVRFRISATEGFDLSPPDPA
CCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
LWPEITAPAVDGVQRINLDTLTESAKQSWKAGDTLLLSGRILTGRDAAHKRLDEMLDRGD
CCCCCCCHHHCCHHHCCHHHHHHHHHHHHCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
PLPVDLRGRAIYYVGPVDPAPGEIIGPAGPTTSTRMDKFTPRLLAQTGLGLMIGKAERGP
CCCEEECCCEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCH
DVIEAIARHKAPYLIAVGGAAVLISRAIRNARVVAFEDLGMEAIHEFEVQDLPVIVAVDT
HHHHHHHHCCCCEEEEECHHHHHHHHHHHCCEEEEEHHCCHHHHHHCCCCCCCEEEEEEC
AGTSIHDTGPAQWRRTSTSRSS
CCCCCCCCCCHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]