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The map label for this gene is pyrA

Identifier: 109948116

GI number: 109948116

Start: 1425609

End: 1426748

Strand: Reverse

Name: pyrA

Synonym: Hac_1638

Alternate gene names: 109948116

Gene position: 1426748-1425609 (Counterclockwise)

Preceding gene: 109948117

Following gene: 109948080

Centisome position: 91.82

GC content: 41.14

Gene sequence:

>1140_bases
ATGGTCTCTCTCTATTTAGAAAATGGGCTTTTTTTACAAGTGCAAAGTTTTGGGGCTAGCGGTACGCAAGTGGGGGAGCT
TGTTTTTAACACTTCCATGAGTGGCTATCAAGAAGTCATTAGCGATCCTAGCTATAAAGGGCAATTTGTGATTTTTAGCA
TGCCAGAAATTGGGGTCGTGGGTGCTAATTCTAAAGATGATGAATCCTTTTTTTCAAGTGCAGGGATTTTAGTGCGCCAT
TACAACGAATTTTTTTCTAACTCAAGGGCGGATTTTAGCTTGAGCGCTTATTTGAAAGAGCGTGGCGTTTTAGGGATTTG
TGGGGTTGATACTAGGAGTTTGATTAAAACTTTACGCCATCATGGGTGCTTGATGATGGTCGCTTCCACGATAGAGCATG
ATAAAAACAAGCTTGAAGAGATTCTAAAAAACGCCCCTAGAATTTCTCACACCCCTTTAGTGGCTAGCGTTTCTACACCA
AAAATAATAACCCATCAGCGCGCTACTTTTGATTTCAAAACCTTAGATTACAAGCCTTTTGATGAAAAAAACTCTCATAA
AACTATCGCTGTGCTAGACTTTGGGGCTAAGGGCAATATTTTAAATGAGCTTCAAAATGTGGGGTTAAAAGCCCTTATTT
ATCCGCACCACACCAAAGCTAACGAGCTTATCAAAGCTTATGAAAAAAAAGAAATCCATGGGATTTTCCTCTCTAATGGG
CCAGGCGATCCTTTGAGTTTGCAACAAGAAATTGGAGAAATCAAACAACTCATTGGCGCTAAAATCCCTATGTTTGGCAT
TTGTTTAGGGCATCAACTGCTCTCTATCGCGCAAGGCTACCCTACCTACAAGCTCAAATTTGGCCACCATGGGAGCAACC
ACCCTGTCAAAAACCTAGAAACAAACGCCGTTGAAATCACTGCACAAAACCACAACTATTGTGTCCCTGAAGCAATTGAA
AAAATCGCTACTATCACACACCGCAACCTTTTTGACAACACCATTGAAGGCGTGCACTATAAAAACGCCCCCATCATCTC
TGTCCAGCACCACCCAGAAAGTAGCCCCGGTCCTAAAGAGAGCCATTATATTTTTAAAGAATTTGTGAAATTGTTAGAGA
ATTTCCCCACTAGAGAATAA

Upstream 100 bases:

>100_bases
TCAAGCATTACAAACGCAAATTACCCGTAGGGAGCGATGAATACGAACTCGTGTTTGAAAGGCTTTATGAAGAAGAATTA
AGACGCAAAGGCTTTTTGTA

Downstream 100 bases:

>100_bases
GAGATATCTTTTCACAGAAATTTTAGTGGTTGTTTATATCTCTGAAACCCCTAATACCCGCATTTTTGAAAATGAAACCT
TTTTATTCACCGTCTCTTCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MVSLYLENGLFLQVQSFGASGTQVGELVFNTSMSGYQEVISDPSYKGQFVIFSMPEIGVVGANSKDDESFFSSAGILVRH
YNEFFSNSRADFSLSAYLKERGVLGICGVDTRSLIKTLRHHGCLMMVASTIEHDKNKLEEILKNAPRISHTPLVASVSTP
KIITHQRATFDFKTLDYKPFDEKNSHKTIAVLDFGAKGNILNELQNVGLKALIYPHHTKANELIKAYEKKEIHGIFLSNG
PGDPLSLQQEIGEIKQLIGAKIPMFGICLGHQLLSIAQGYPTYKLKFGHHGSNHPVKNLETNAVEITAQNHNYCVPEAIE
KIATITHRNLFDNTIEGVHYKNAPIISVQHHPESSPGPKESHYIFKEFVKLLENFPTRE

Sequences:

>Translated_379_residues
MVSLYLENGLFLQVQSFGASGTQVGELVFNTSMSGYQEVISDPSYKGQFVIFSMPEIGVVGANSKDDESFFSSAGILVRH
YNEFFSNSRADFSLSAYLKERGVLGICGVDTRSLIKTLRHHGCLMMVASTIEHDKNKLEEILKNAPRISHTPLVASVSTP
KIITHQRATFDFKTLDYKPFDEKNSHKTIAVLDFGAKGNILNELQNVGLKALIYPHHTKANELIKAYEKKEIHGIFLSNG
PGDPLSLQQEIGEIKQLIGAKIPMFGICLGHQLLSIAQGYPTYKLKFGHHGSNHPVKNLETNAVEITAQNHNYCVPEAIE
KIATITHRNLFDNTIEGVHYKNAPIISVQHHPESSPGPKESHYIFKEFVKLLENFPTRE
>Mature_379_residues
MVSLYLENGLFLQVQSFGASGTQVGELVFNTSMSGYQEVISDPSYKGQFVIFSMPEIGVVGANSKDDESFFSSAGILVRH
YNEFFSNSRADFSLSAYLKERGVLGICGVDTRSLIKTLRHHGCLMMVASTIEHDKNKLEEILKNAPRISHTPLVASVSTP
KIITHQRATFDFKTLDYKPFDEKNSHKTIAVLDFGAKGNILNELQNVGLKALIYPHHTKANELIKAYEKKEIHGIFLSNG
PGDPLSLQQEIGEIKQLIGAKIPMFGICLGHQLLSIAQGYPTYKLKFGHHGSNHPVKNLETNAVEITAQNHNYCVPEAIE
KIATITHRNLFDNTIEGVHYKNAPIISVQHHPESSPGPKESHYIFKEFVKLLENFPTRE

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=388, Percent_Identity=32.2164948453608, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=31.5926892950392, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=31.5926892950392, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI1786215, Length=380, Percent_Identity=40.2631578947368, Blast_Score=275, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI193204318, Length=381, Percent_Identity=31.2335958005249, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=35.1282051282051, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322331, Length=406, Percent_Identity=31.5270935960591, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24642586, Length=396, Percent_Identity=30.5555555555556, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45555749, Length=396, Percent_Identity=30.5555555555556, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_HELAH (Q17VI1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665344.1
- ProteinModelPortal:   Q17VI1
- SMR:   Q17VI1
- STRING:   Q17VI1
- GeneID:   4177748
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1638
- NMPDR:   fig|382638.8.peg.1585
- eggNOG:   COG0505
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   HACI382638:HAC_1638-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42247; Mature: 42247

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 268-268 ACT_SITE 351-351 ACT_SITE 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLYLENGLFLQVQSFGASGTQVGELVFNTSMSGYQEVISDPSYKGQFVIFSMPEIGVV
CEEEEEECCEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCCEEE
GANSKDDESFFSSAGILVRHYNEFFSNSRADFSLSAYLKERGVLGICGVDTRSLIKTLRH
CCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEHHHHHHHCCCEEEECCCHHHHHHHHHH
HGCLMMVASTIEHDKNKLEEILKNAPRISHTPLVASVSTPKIITHQRATFDFKTLDYKPF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCEEEEEECCCCCC
DEKNSHKTIAVLDFGAKGNILNELQNVGLKALIYPHHTKANELIKAYEKKEIHGIFLSNG
CCCCCCCEEEEEEECCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEECC
PGDPLSLQQEIGEIKQLIGAKIPMFGICLGHQLLSIAQGYPTYKLKFGHHGSNHPVKNLE
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
TNAVEITAQNHNYCVPEAIEKIATITHRNLFDNTIEGVHYKNAPIISVQHHPESSPGPKE
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCH
SHYIFKEFVKLLENFPTRE
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVSLYLENGLFLQVQSFGASGTQVGELVFNTSMSGYQEVISDPSYKGQFVIFSMPEIGVV
CEEEEEECCEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCCEEE
GANSKDDESFFSSAGILVRHYNEFFSNSRADFSLSAYLKERGVLGICGVDTRSLIKTLRH
CCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEHHHHHHHCCCEEEECCCHHHHHHHHHH
HGCLMMVASTIEHDKNKLEEILKNAPRISHTPLVASVSTPKIITHQRATFDFKTLDYKPF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCEEEEEECCCCCC
DEKNSHKTIAVLDFGAKGNILNELQNVGLKALIYPHHTKANELIKAYEKKEIHGIFLSNG
CCCCCCCEEEEEEECCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEECC
PGDPLSLQQEIGEIKQLIGAKIPMFGICLGHQLLSIAQGYPTYKLKFGHHGSNHPVKNLE
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
TNAVEITAQNHNYCVPEAIEKIATITHRNLFDNTIEGVHYKNAPIISVQHHPESSPGPKE
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCH
SHYIFKEFVKLLENFPTRE
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA