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Identifier: 109947463

GI number: 109947463

Start: 801555

End: 802082

Strand: Reverse

Name: def

Synonym: Hac_0915

Alternate gene names: 109947463

Gene position: 802082-801555 (Counterclockwise)

Preceding gene: 109947464

Following gene: 109947462

Centisome position: 51.62

GC content: 39.96

Gene sequence:

>528_bases
ATGGCGTTATTAGAGATTATCCATTACCCTTCTAAAATCTTAAGAACGATTTCTAAAGAGGTCGTTTCTTTTGATGCAAA
ATTCCATCAGCAATTAGATGACATGCATGAAACTATGATCGCTAGTGAGGGGATAGGGTTAGCGGCGATTCAAGTGGGCT
TGCCTTTGAGAATGTTGCTTATCAATCTCCCACGAGAAGATGGCGTGCAGCATAAAGAGGATTGCCTAGAAATCATTAAC
CCCAAGTTTATAGAAACTAAAGGATCAATCATGTTTAAAGAGGGGTGCTTGTCTGTGCCGGGGTTTTATGAAGAAGTGGA
GCGCTTTGAAAAGGTTAAGATAGAGTATCAAAACCGCTTCGCTAAAGTGAAAGTTTTAGAGGCGAGCGAGTTGTTAGCGG
TAGCCATCCAGCATGAGATAGACCATCTCAATGGCGTGCTGTTTGTGGATAAATTGTCCATCTTGAAGCGTAAGAAATTT
GAAAAAGAACTCAAAGAATTAAATAAAAATCCCAAAAACAAGTCCTAG

Upstream 100 bases:

>100_bases
ATCGCTAAAGACACGGATAGGGATTTTTACATGAGCGCTTTAGAAGCTAAAGAGTATGGCTTGATTGATAAGGTGTTGGA
GAAAAATGTAAAGTGATTGC

Downstream 100 bases:

>100_bases
CCATGATTAACACGATATTTTGCACAACCATGCAAAGGGGAGTAGCAGAAATCGTGGCTGTGGAAGCGACTTTCACGAGA
GCTTTACCGGCATTTGTGAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MALLEIIHYPSKILRTISKEVVSFDAKFHQQLDDMHETMIASEGIGLAAIQVGLPLRMLLINLPREDGVQHKEDCLEIIN
PKFIETKGSIMFKEGCLSVPGFYEEVERFEKVKIEYQNRFAKVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKF
EKELKELNKNPKNKS

Sequences:

>Translated_175_residues
MALLEIIHYPSKILRTISKEVVSFDAKFHQQLDDMHETMIASEGIGLAAIQVGLPLRMLLINLPREDGVQHKEDCLEIIN
PKFIETKGSIMFKEGCLSVPGFYEEVERFEKVKIEYQNRFAKVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKF
EKELKELNKNPKNKS
>Mature_174_residues
ALLEIIHYPSKILRTISKEVVSFDAKFHQQLDDMHETMIASEGIGLAAIQVGLPLRMLLINLPREDGVQHKEDCLEIINP
KFIETKGSIMFKEGCLSVPGFYEEVERFEKVKIEYQNRFAKVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFE
KELKELNKNPKNKS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=41.4201183431953, Blast_Score=128, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.6455696202532, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_HELAH (Q17XD4)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664691.1
- ProteinModelPortal:   Q17XD4
- SMR:   Q17XD4
- STRING:   Q17XD4
- GeneID:   4177124
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0915
- NMPDR:   fig|382638.8.peg.889
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   ETMIASE
- PhylomeDB:   Q17XD4
- ProtClustDB:   PRK00150
- BioCyc:   HACI382638:HAC_0915-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20116; Mature: 19985

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEIIHYPSKILRTISKEVVSFDAKFHQQLDDMHETMIASEGIGLAAIQVGLPLRMLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHH
INLPREDGVQHKEDCLEIINPKFIETKGSIMFKEGCLSVPGFYEEVERFEKVKIEYQNRF
HCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AKVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFEKELKELNKNPKNKS
HHEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALLEIIHYPSKILRTISKEVVSFDAKFHQQLDDMHETMIASEGIGLAAIQVGLPLRMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHH
INLPREDGVQHKEDCLEIINPKFIETKGSIMFKEGCLSVPGFYEEVERFEKVKIEYQNRF
HCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AKVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFEKELKELNKNPKNKS
HHEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA