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The map label for this gene is dus [H]

Identifier: 109947333

GI number: 109947333

Start: 668738

End: 669724

Strand: Direct

Name: dus [H]

Synonym: Hac_0770

Alternate gene names: 109947333

Gene position: 668738-669724 (Clockwise)

Preceding gene: 109947332

Following gene: 109947334

Centisome position: 43.04

GC content: 39.61

Gene sequence:

>987_bases
ATGGACTTTAAGAATAAAAAATGGCTTTTTCTAGCCCCTCTAGCAGGCTATACGGATTTGCCTTTTAGAAGCGTGGTGAA
AAAATTTGGCGTGGATGTGACCACAAGCGAAATGGTGAGCTCGCATTCGCTAGTGTATGCGTTTGATAAAACTTCTAAAA
TGTTAGAAAAATCCACTTTAGAAGATCATTTCATGGTGCAAATTTCAGGCTCTAAAGAAAGCGTAGTCAAAGAAGCGGTT
GAAAAAATCAACGCTTTAGAGCATGTGAGTGGGATTGATTTTAATTGTGGTTGTCCTGCCCCCAAAGTGGCTAATCATGG
TAATGGTAGCGGATTGTTAAAGGATTTAAACCACTTGGTGAAACTCTTAAAAGTGATTAGAGAAAACACCAACAAACCCA
TCACAAGCGTGAAAGTGCGTTTGGGTTTTGAAAAGAAAATCCCCAAAGAAATCGCTCATGCACTCAATGACGCTCCAATT
GATTATGTGGTGGTGCATGGAAGGACACGAAGCGATAAATACCAAAAAGATAAAATAGATTATGAAAGCATCGCCTTAAT
GAAAGAGATTTTAAAAAAGCCTGTGATAGCCAATGGCGAAATTGACAGCGTGAAAAAAGCCTTTGAAGTCTTGCAAATCA
CGCAAGCTGATGGGCTAATGATAGGGCGAGCAGCCTTAAGAGCCCCATGGATATTTTGGCAAATTAGGAATAACACCACA
GAATTACCGGCGGTTGTAAAAAAAGACTTGGTTTTAGAACATTTTGATAAAATGGTGGAGTTTTATGGGGATAGGGGGGT
TGTCATGTTTAGGAAAAACTTGCATGCTTACGCTAAGGGCGAAATGCAAGCGAGTGCGTTTCGTAATTGCGTCAATACCC
TTACAGAAATAAAGAGCATGCGAGAGAGCATAGAGGAATTTTTTAATCAAGAAATGTTGCAAGGTGAAGTGCCATTATGG
GTAGAATTGAATCAAAAAAGCGTTTGA

Upstream 100 bases:

>100_bases
ATAGAGTGGGTTAAAAGTAAGTATTGATTGGGTTAGTTTTTAAAGCGCTTTTTAACCTTGATTGTGTCAAAATACAGCAA
AAAATTTTAATTGAGAGCGT

Downstream 100 bases:

>100_bases
GGGCGGTTATTTTTTTAGCCAGTTTGGGGGTTTTGTGGGGTAGTGCTAGTGAAAAAACGCCTTTTTTTAAAACTAAAAAT
CATATCTATTTGGGTTTTAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSTLEDHFMVQISGSKESVVKEAV
EKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKVIRENTNKPITSVKVRLGFEKKIPKEIAHALNDAPI
DYVVVHGRTRSDKYQKDKIDYESIALMKEILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
ELPAVVKKDLVLEHFDKMVEFYGDRGVVMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQGEVPLW
VELNQKSV

Sequences:

>Translated_328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSTLEDHFMVQISGSKESVVKEAV
EKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKVIRENTNKPITSVKVRLGFEKKIPKEIAHALNDAPI
DYVVVHGRTRSDKYQKDKIDYESIALMKEILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
ELPAVVKKDLVLEHFDKMVEFYGDRGVVMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQGEVPLW
VELNQKSV
>Mature_328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSTLEDHFMVQISGSKESVVKEAV
EKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKVIRENTNKPITSVKVRLGFEKKIPKEIAHALNDAPI
DYVVVHGRTRSDKYQKDKIDYESIALMKEILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
ELPAVVKKDLVLEHFDKMVEFYGDRGVVMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQGEVPLW
VELNQKSV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=243, Percent_Identity=30.8641975308642, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI239788462, Length=243, Percent_Identity=30.8641975308642, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=28.125, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=26.8, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=315, Percent_Identity=34.2857142857143, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1788462, Length=245, Percent_Identity=32.6530612244898, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17543114, Length=276, Percent_Identity=27.8985507246377, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=27.9816513761468, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=28.9473684210526, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17510279, Length=248, Percent_Identity=27.0161290322581, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=265, Percent_Identity=26.7924528301887, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=24.7826086956522, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=257, Percent_Identity=24.124513618677, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585320, Length=342, Percent_Identity=27.4853801169591, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=27.5555555555556, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24580595, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920448, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37147; Mature: 37147

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00435 PEROXIDASE_1 ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECHHHHHHHHHCC
EDHFMVQISGSKESVVKEAVEKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLV
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLLKVIRENTNKPITSVKVRLGFEKKIPKEIAHALNDAPIDYVVVHGRTRSDKYQKDKID
HHHHHHHHCCCCCCEEEEEEECCHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCC
YESIALMKEILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCEEEEEECCCCC
ELPAVVKKDLVLEHFDKMVEFYGDRGVVMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSM
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RESIEEFFNQEMLQGEVPLWVELNQKSV
HHHHHHHHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECHHHHHHHHHCC
EDHFMVQISGSKESVVKEAVEKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLV
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLLKVIRENTNKPITSVKVRLGFEKKIPKEIAHALNDAPIDYVVVHGRTRSDKYQKDKID
HHHHHHHHCCCCCCEEEEEEECCHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCC
YESIALMKEILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCEEEEEECCCCC
ELPAVVKKDLVLEHFDKMVEFYGDRGVVMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSM
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RESIEEFFNQEMLQGEVPLWVELNQKSV
HHHHHHHHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]