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The map label for this gene is papS [H]

Identifier: 109947332

GI number: 109947332

Start: 667507

End: 668664

Strand: Direct

Name: papS [H]

Synonym: Hac_0768

Alternate gene names: 109947332

Gene position: 667507-668664 (Clockwise)

Preceding gene: 109947323

Following gene: 109947333

Centisome position: 42.96

GC content: 34.46

Gene sequence:

>1158_bases
GTGTTTGAGTTAGAAGAAGAATTAAAAGAATTGTTTGATCTTTATGAAAAATCCCATTATGAGCTTTACTTGGTGGGAGG
GTGCGTGCGCGATTGTTTAATGGGCTTTGCCCCTAAAGATTATGACTTGACTTCAAACGCTTTAGTGAGTGAAAGTAAAG
AACTTCTTTTAAAACACCATTTTAGGGTGCTAGAAACCGGTATCAAACATGGCACGATCACAGCCCTTAAAAACAATAAA
AGCTATGAAATCACAACTTTTAGGGTTGAAAAGGGGCATATTAAGCACAGAAGACCTAAAGAATTGGTTTTTAGCGCTCA
TTTAACAGACGATTTAAAACGGCGCGATTTTAGCATGAACGCCATCGCTTATAGCCCTACAAAAGGCTTAATTGATCCTT
TTAAAGGGCAAAATGCGATTGAAAATCGCATGATTGAATGCGTGGGCAAAGTGCGATTAAGGTTTTTTGAAGACGCTTTA
AGGATTTTAAGGGCGTTACGATTTGGTGCGACTTTAGGGTTTAAGATAGAAACAAACACCAAAAGAGCGGTTTTTGCATG
CAAGGATTTGTTAAAACACCTTTCCAAAGAACGCTTGCAAAGCGAATTGAATAAGCTTCTTATGGGGAAAAACGCCTATG
GAGTCATTAAAGAGTATCAAGAAATTTTAGAGTTGGTTATTCAAGAGCGTTTAGAAAATGTAGAGTTTTTAAAAAACGCC
CCCTTTAATTTAGAATTAAGATTATTAGGGTTTTTTACGCATCAAAAAAGTTTAGAAAATTTACGCTACCCTAAAAAAAT
GATCATTTTATTCGCTAAAGCTAAAGAATGCCACCAAGCTTTTTTGAATATCCACAATAAAACAGAATTAAAATTTTTAT
TAAAAAACTATGATTTAGAGCCTTTTAATTTGGCTTTAGATTTTTATGCGTTAGAAAACCCCAAACACGCCTTAAAAATC
AAAGGCTTGTTAAAAGAAATCTTTAACGCTAATGAGCCTTTTAAAAAAGAACACCTAGTCCTTAAAGGCGGTGCACTTAA
AAACTACCAGCACCAAAAAATAGGCGAAATTTTAAACGCATGCTTAGATCTAGTCATTACAAACCCTAAAAACAATTCTT
TAGAGTTTTTGATAGAGTGGGTTAAAAGTAAGTATTGA

Upstream 100 bases:

>100_bases
GTCCGCCACTAAAACTAATCACACAAATTTCTTGTCCCATCAAAACCCCTTAAAATTCTAAAAAGATATAATAACACAAA
GAAATTGAAGAGCAAAAAGC

Downstream 100 bases:

>100_bases
TTGGGTTAGTTTTTAAAGCGCTTTTTAACCTTGATTGTGTCAAAATACAGCAAAAAATTTTAATTGAGAGCGTATGGACT
TTAAGAATAAAAAATGGCTT

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MFELEEELKELFDLYEKSHYELYLVGGCVRDCLMGFAPKDYDLTSNALVSESKELLLKHHFRVLETGIKHGTITALKNNK
SYEITTFRVEKGHIKHRRPKELVFSAHLTDDLKRRDFSMNAIAYSPTKGLIDPFKGQNAIENRMIECVGKVRLRFFEDAL
RILRALRFGATLGFKIETNTKRAVFACKDLLKHLSKERLQSELNKLLMGKNAYGVIKEYQEILELVIQERLENVEFLKNA
PFNLELRLLGFFTHQKSLENLRYPKKMIILFAKAKECHQAFLNIHNKTELKFLLKNYDLEPFNLALDFYALENPKHALKI
KGLLKEIFNANEPFKKEHLVLKGGALKNYQHQKIGEILNACLDLVITNPKNNSLEFLIEWVKSKY

Sequences:

>Translated_385_residues
MFELEEELKELFDLYEKSHYELYLVGGCVRDCLMGFAPKDYDLTSNALVSESKELLLKHHFRVLETGIKHGTITALKNNK
SYEITTFRVEKGHIKHRRPKELVFSAHLTDDLKRRDFSMNAIAYSPTKGLIDPFKGQNAIENRMIECVGKVRLRFFEDAL
RILRALRFGATLGFKIETNTKRAVFACKDLLKHLSKERLQSELNKLLMGKNAYGVIKEYQEILELVIQERLENVEFLKNA
PFNLELRLLGFFTHQKSLENLRYPKKMIILFAKAKECHQAFLNIHNKTELKFLLKNYDLEPFNLALDFYALENPKHALKI
KGLLKEIFNANEPFKKEHLVLKGGALKNYQHQKIGEILNACLDLVITNPKNNSLEFLIEWVKSKY
>Mature_385_residues
MFELEEELKELFDLYEKSHYELYLVGGCVRDCLMGFAPKDYDLTSNALVSESKELLLKHHFRVLETGIKHGTITALKNNK
SYEITTFRVEKGHIKHRRPKELVFSAHLTDDLKRRDFSMNAIAYSPTKGLIDPFKGQNAIENRMIECVGKVRLRFFEDAL
RILRALRFGATLGFKIETNTKRAVFACKDLLKHLSKERLQSELNKLLMGKNAYGVIKEYQEILELVIQERLENVEFLKNA
PFNLELRLLGFFTHQKSLENLRYPKKMIILFAKAKECHQAFLNIHNKTELKFLLKNYDLEPFNLALDFYALENPKHALKI
KGLLKEIFNANEPFKKEHLVLKGGALKNYQHQKIGEILNACLDLVITNPKNNSLEFLIEWVKSKY

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=215, Percent_Identity=33.953488372093, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=31.304347826087, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789436, Length=197, Percent_Identity=30.4568527918782, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=223, Percent_Identity=35.8744394618834, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321016, Length=265, Percent_Identity=26.4150943396226, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI281360112, Length=209, Percent_Identity=34.9282296650718, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21357337, Length=209, Percent_Identity=34.9282296650718, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44880; Mature: 44880

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELEEELKELFDLYEKSHYELYLVGGCVRDCLMGFAPKDYDLTSNALVSESKELLLKHH
CCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
FRVLETGIKHGTITALKNNKSYEITTFRVEKGHIKHRRPKELVFSAHLTDDLKRRDFSMN
HHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCHHHEEEHHHHHHHHHCCCCCC
AIAYSPTKGLIDPFKGQNAIENRMIECVGKVRLRFFEDALRILRALRFGATLGFKIETNT
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
KRAVFACKDLLKHLSKERLQSELNKLLMGKNAYGVIKEYQEILELVIQERLENVEFLKNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PFNLELRLLGFFTHQKSLENLRYPKKMIILFAKAKECHQAFLNIHNKTELKFLLKNYDLE
CCCEEEEEEEHHHHHHHHHHCCCCHHHEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
PFNLALDFYALENPKHALKIKGLLKEIFNANEPFKKEHLVLKGGALKNYQHQKIGEILNA
CHHEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
CLDLVITNPKNNSLEFLIEWVKSKY
HHHHEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFELEEELKELFDLYEKSHYELYLVGGCVRDCLMGFAPKDYDLTSNALVSESKELLLKHH
CCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
FRVLETGIKHGTITALKNNKSYEITTFRVEKGHIKHRRPKELVFSAHLTDDLKRRDFSMN
HHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCHHHEEEHHHHHHHHHCCCCCC
AIAYSPTKGLIDPFKGQNAIENRMIECVGKVRLRFFEDALRILRALRFGATLGFKIETNT
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
KRAVFACKDLLKHLSKERLQSELNKLLMGKNAYGVIKEYQEILELVIQERLENVEFLKNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PFNLELRLLGFFTHQKSLENLRYPKKMIILFAKAKECHQAFLNIHNKTELKFLLKNYDLE
CCCEEEEEEEHHHHHHHHHHCCCCHHHEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
PFNLALDFYALENPKHALKIKGLLKEIFNANEPFKKEHLVLKGGALKNYQHQKIGEILNA
CHHEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
CLDLVITNPKNNSLEFLIEWVKSKY
HHHHEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA