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The map label for this gene is ISHa1942 tnpB fragment 2 [H]

Identifier: 109947323

GI number: 109947323

Start: 661717

End: 662610

Strand: Direct

Name: ISHa1942 tnpB fragment 2 [H]

Synonym: Hac_0756

Alternate gene names: 109947323

Gene position: 661717-662610 (Clockwise)

Preceding gene: 109947322

Following gene: 109947332

Centisome position: 42.58

GC content: 32.77

Gene sequence:

>894_bases
ATGTGCATGCATAGAAACTTCCCTCACAATTCTAAAGTCAAACAAATTGTGATCTCTTGCAACCATAGAAAATATTTTGT
TAGCTTTAGCGTGGAATACAAACAAGACATTACTCCCATCAAAAACCCTAAAGATTGTGTGGGACTAGATTTGAATATCC
TTGATATAGCTTGTTCTTGTGGTGTGAATAATCACAAAAAACTAACGGATTTTAAGCGATACCAAATAGACATGAAAGAA
TTACTAGGGATAGAAATAGATGAAGAGTTGGATACTAAACGACTTATCTCTACTTATTCCAAATTGTATTCTTTAAAAAA
ATACTCTAGAGAATGTAAAAGATTACAAAGAAAACAAAGCCGTAGGGCGTTAAAGTCTAAACAAAACAAAACCAAATTAG
GAGGTAATTTTTACAAAACCCAAAAGAAATTAAACCAAGCCTTTGACAAGTCTAGTCATCAAAAAACAGACAGATACCAT
AAAATCACAAGCGAACTTTCAAAGCAATTTGAATTGATAGTAGTTGAAGACTTGCAAGTAAAAAACATGACTAAAAGAGC
TAAACTCAAAAATGTTAAACAAAAGAACGCACTTAATAGATCTATCTTAAACGCTTCATTCTATCAAATCATCTCTTTTT
TAGACTACAAACAACAGCATAATGGCAAATTGTTAGTAAAAGTTCCCCCACAATATACGAGTAAGACTTGCCATTGTTGT
GGGAATATCAACTACAAGCTTAAATTGAACCATAGGCAATATTGGTGTTTAGAATGCGGGTATAGAGAACACAGAGACAT
CAACGCTGCGAATAATATTTTAAGCAAAGGGTTAAGTCTTTTTGGGGTAGGAAATATCCATGCAGACTTTAAAGAACAAA
GTCTTTCGTGTTAG

Upstream 100 bases:

>100_bases
GCTAAACAATCTTTTTCGTGGAACAATCAAGGTTTCTCTATTAAGGAGAGCGATGATGAACGCTTTAAGATATTCACTTT
AATGAAAATGCCCTTTAATG

Downstream 100 bases:

>100_bases
TGTCCATTAGGGCGCTTTAGTTAGGGGTGGTTTCACTAGTGTGGGTTCGGTTTCTTTTTCAAACTCATGTGGTAATTTTT
CTGTAATTTCTTTGAATTTT

Product: ISHa1942 transposase B fragment 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MCMHRNFPHNSKVKQIVISCNHRKYFVSFSVEYKQDITPIKNPKDCVGLDLNILDIACSCGVNNHKKLTDFKRYQIDMKE
LLGIEIDEELDTKRLISTYSKLYSLKKYSRECKRLQRKQSRRALKSKQNKTKLGGNFYKTQKKLNQAFDKSSHQKTDRYH
KITSELSKQFELIVVEDLQVKNMTKRAKLKNVKQKNALNRSILNASFYQIISFLDYKQQHNGKLLVKVPPQYTSKTCHCC
GNINYKLKLNHRQYWCLECGYREHRDINAANNILSKGLSLFGVGNIHADFKEQSLSC

Sequences:

>Translated_297_residues
MCMHRNFPHNSKVKQIVISCNHRKYFVSFSVEYKQDITPIKNPKDCVGLDLNILDIACSCGVNNHKKLTDFKRYQIDMKE
LLGIEIDEELDTKRLISTYSKLYSLKKYSRECKRLQRKQSRRALKSKQNKTKLGGNFYKTQKKLNQAFDKSSHQKTDRYH
KITSELSKQFELIVVEDLQVKNMTKRAKLKNVKQKNALNRSILNASFYQIISFLDYKQQHNGKLLVKVPPQYTSKTCHCC
GNINYKLKLNHRQYWCLECGYREHRDINAANNILSKGLSLFGVGNIHADFKEQSLSC
>Mature_297_residues
MCMHRNFPHNSKVKQIVISCNHRKYFVSFSVEYKQDITPIKNPKDCVGLDLNILDIACSCGVNNHKKLTDFKRYQIDMKE
LLGIEIDEELDTKRLISTYSKLYSLKKYSRECKRLQRKQSRRALKSKQNKTKLGGNFYKTQKKLNQAFDKSSHQKTDRYH
KITSELSKQFELIVVEDLQVKNMTKRAKLKNVKQKNALNRSILNASFYQIISFLDYKQQHNGKLLVKVPPQYTSKTCHCC
GNINYKLKLNHRQYWCLECGYREHRDINAANNILSKGLSLFGVGNIHADFKEQSLSC

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=280, Percent_Identity=28.9285714285714, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 34770; Mature: 34770

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMHRNFPHNSKVKQIVISCNHRKYFVSFSVEYKQDITPIKNPKDCVGLDLNILDIACSC
CCCCCCCCCCCHHHEEEEEECCEEEEEEEEEEHHHCCCCCCCCCHHHCCCCHHHHHHHHC
GVNNHKKLTDFKRYQIDMKELLGIEIDEELDTKRLISTYSKLYSLKKYSRECKRLQRKQS
CCCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRALKSKQNKTKLGGNFYKTQKKLNQAFDKSSHQKTDRYHKITSELSKQFELIVVEDLQV
HHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCH
KNMTKRAKLKNVKQKNALNRSILNASFYQIISFLDYKQQHNGKLLVKVPPQYTSKTCHCC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHC
GNINYKLKLNHRQYWCLECGYREHRDINAANNILSKGLSLFGVGNIHADFKEQSLSC
CCCCEEEEECCCEEEEEECCCHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHHCCCC
>Mature Secondary Structure
MCMHRNFPHNSKVKQIVISCNHRKYFVSFSVEYKQDITPIKNPKDCVGLDLNILDIACSC
CCCCCCCCCCCHHHEEEEEECCEEEEEEEEEEHHHCCCCCCCCCHHHCCCCHHHHHHHHC
GVNNHKKLTDFKRYQIDMKELLGIEIDEELDTKRLISTYSKLYSLKKYSRECKRLQRKQS
CCCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRALKSKQNKTKLGGNFYKTQKKLNQAFDKSSHQKTDRYHKITSELSKQFELIVVEDLQV
HHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCH
KNMTKRAKLKNVKQKNALNRSILNASFYQIISFLDYKQQHNGKLLVKVPPQYTSKTCHCC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHC
GNINYKLKLNHRQYWCLECGYREHRDINAANNILSKGLSLFGVGNIHADFKEQSLSC
CCCCEEEEECCCEEEEEECCCHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]