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The map label for this gene is hyaB [H]

Identifier: 109947313

GI number: 109947313

Start: 653775

End: 655511

Strand: Direct

Name: hyaB [H]

Synonym: Hac_0745

Alternate gene names: 109947313

Gene position: 653775-655511 (Clockwise)

Preceding gene: 109947312

Following gene: 109947314

Centisome position: 42.07

GC content: 41.16

Gene sequence:

>1737_bases
ATGTCAAAAAAAATAGTAGTTGATCCTATCACTAGGATTGAGGGGCATTTAAGGATTGAGGTTATCGTAGATGATAATAA
TGTGATTACTGATGCGTTTTCTTCCTCTACGCTTTTTAGGGGGCTAGAGACGATCATTAAGGGCAGAGACCCACGAGATG
CGGGCTTTATCGCTCAAAGGATTTGTGGAGTGTGCACTTATTCGCATTATAAAGCCGGTGTTACTGCAGTAGAAAACGCT
CTGGGGATTACCCCTCCATTGAACGCGCAGTTGGTACGATCTTTGATGAACATGGCGTTGCTTTTTCACGATCATGTGGT
GCATTTTTATACTTTGCATGGGCTTGATTGGTGCGATATTTTGAGCGCTTTAAAAGCAGACCCTATTGAAGCTTCAAAAC
TTGCTTTTAAATACAGCCCTTATCCTATTAATACCGGTGCTGGCGAATTAAAAGCGGTTCAAAAACGCTTGAATGATTTT
GCTAAAGGCGGATCTTTAGGGCCTTTTAGTAATGGCTATTATGGGCATAAGACTTATCGTTTAAGTCCAGAGCAAAATTT
AATCGTCTTAAGCCACTATCTCAAGCTTTTAGAAATCCAAAGAGAAGCGGCGAAAATGACCGCTATTTTTGGGGCTAAAC
AACCCCACCCACAAAGCTTAACGGTAGGGGGTGTTACGAGCGTGATGGATATACTAGATCCTACGAGATTGGCGGAGTGG
AAGAGCAAGTTTGAAGTGGTGGCCCATTTCATTAACCATGCTTACTACCCTGATTTGGTGATGGCAGGCGAAATGTTTGC
TAATGAACAATCGGTCATTAAAGGCTGTGGCTTAAAGAATTTTATCGCTTATGAAGAAGTGTTGCTTGGGAGGGATAAAT
ACCTTTTGAGTAGTGGGGTGGTGCTTGATGGGGATATTTCTAAATTACACCCTATTAATGAAAGCTTGATTAAAGAAGAG
GTTACGCATTCTTGGTATCAATATGAAGACACTAAAGAAGTGCAACTCCACCCTTATGATGGGCAAACTAATCCGCATTA
TACCGGTTTTAAAGATGGCGAGAGCGTGGGGATTGAAAATAAAATGATCCCTACTAAAGTGCTTGACACTAAAAATAAAT
ATTCTTGGATCAAATCGCCCAGATACGATAATATGCCTATGGAAGTAGGCCCTTTAAGCGCGGTAGTGGTGGGTTTAGCG
GCTAAAAACCCCTATATTACTGAAGTGGCTACTAAGTTTTTAAAAGACACTAAATTGCCTTTAGAGGCGTTGTTTTCAAC
GCTTGGGCGAACGGCAGCAAGGTGTATTGAAGCTAAAACGATTGCTGATAATGGTCTTTTAGCGTTTGATGCGTTAGTGG
AAAATCTAAAAAGCGATCAAAGCACTTGCACTCCTTATGAAATTGATAAAAATCAAGAATATAAAGGGCGCTATATTGGT
CAAGTGCCAAGGGGCATGCTAAGCCATTGGGTACGTATTAAAAACGGCGTAGTAGAAAATTATCAAGCGGTCGTGCCTTC
TACTTGGAATGCGGGGCCTAGGGACTCTCAAGGTCAAAGGGGGGCTTATGAAATGAGTTTGATTGGCACTAGAATCGCTG
ATTTAACCCAACCTTTAGAAATCATTAGAACCATCCATTCTTTTGACCCATGCATCGCATGCTCAGTGCATGTAATGGAT
TTTAAAGGGCAGTCTTTAAACGAGTTTAAAGTAGAGCCTAATTTTGCTAAATTCTAA

Upstream 100 bases:

>100_bases
TAAAGTAGGCACGACTCTACTCAGTGCAGCCGCTATTGGCATTGCTGCACATGCACTTCTTTCTAAAGCGATCAAAAATA
AAGAGCAATAAGGGATTAAG

Downstream 100 bases:

>100_bases
AAAGGGATACGCATGGATAAAAAGGTCGTTTTACACAAAGAGTATTCAGGTTTTGTGCGCTTTTTCCATTGGGTTAGGGC
TTTGAGTATTTTTATTTTAA

Product: hydrogenase large chain

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSKKIVVDPITRIEGHLRIEVIVDDNNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGVTAVENA
LGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDILSALKADPIEASKLAFKYSPYPINTGAGELKAVQKRLNDF
AKGGSLGPFSNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
KSKFEVVAHFINHAYYPDLVMAGEMFANEQSVIKGCGLKNFIAYEEVLLGRDKYLLSSGVVLDGDISKLHPINESLIKEE
VTHSWYQYEDTKEVQLHPYDGQTNPHYTGFKDGESVGIENKMIPTKVLDTKNKYSWIKSPRYDNMPMEVGPLSAVVVGLA
AKNPYITEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCTPYEIDKNQEYKGRYIG
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQGQRGAYEMSLIGTRIADLTQPLEIIRTIHSFDPCIACSVHVMD
FKGQSLNEFKVEPNFAKF

Sequences:

>Translated_578_residues
MSKKIVVDPITRIEGHLRIEVIVDDNNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGVTAVENA
LGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDILSALKADPIEASKLAFKYSPYPINTGAGELKAVQKRLNDF
AKGGSLGPFSNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
KSKFEVVAHFINHAYYPDLVMAGEMFANEQSVIKGCGLKNFIAYEEVLLGRDKYLLSSGVVLDGDISKLHPINESLIKEE
VTHSWYQYEDTKEVQLHPYDGQTNPHYTGFKDGESVGIENKMIPTKVLDTKNKYSWIKSPRYDNMPMEVGPLSAVVVGLA
AKNPYITEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCTPYEIDKNQEYKGRYIG
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQGQRGAYEMSLIGTRIADLTQPLEIIRTIHSFDPCIACSVHVMD
FKGQSLNEFKVEPNFAKF
>Mature_577_residues
SKKIVVDPITRIEGHLRIEVIVDDNNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGVTAVENAL
GITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDILSALKADPIEASKLAFKYSPYPINTGAGELKAVQKRLNDFA
KGGSLGPFSNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEWK
SKFEVVAHFINHAYYPDLVMAGEMFANEQSVIKGCGLKNFIAYEEVLLGRDKYLLSSGVVLDGDISKLHPINESLIKEEV
THSWYQYEDTKEVQLHPYDGQTNPHYTGFKDGESVGIENKMIPTKVLDTKNKYSWIKSPRYDNMPMEVGPLSAVVVGLAA
KNPYITEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCTPYEIDKNQEYKGRYIGQ
VPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQGQRGAYEMSLIGTRIADLTQPLEIIRTIHSFDPCIACSVHVMDF
KGQSLNEFKVEPNFAKF

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=593, Percent_Identity=41.8212478920742, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1789368, Length=595, Percent_Identity=41.1764705882353, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789076, Length=96, Percent_Identity=32.2916666666667, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 64460; Mature: 64328

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIVVDPITRIEGHLRIEVIVDDNNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQR
CCCCEEECCHHHCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGVTAVENALGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
LSALKADPIEASKLAFKYSPYPINTGAGELKAVQKRLNDFAKGGSLGPFSNGYYGHKTYR
HHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCHHHHHHH
KSKFEVVAHFINHAYYPDLVMAGEMFANEQSVIKGCGLKNFIAYEEVLLGRDKYLLSSGV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCC
VLDGDISKLHPINESLIKEEVTHSWYQYEDTKEVQLHPYDGQTNPHYTGFKDGESVGIEN
EEECCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
KMIPTKVLDTKNKYSWIKSPRYDNMPMEVGPLSAVVVGLAAKNPYITEVATKFLKDTKLP
CCCCHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCTPYEIDKNQEYKGRYIG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHH
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQGQRGAYEMSLIGTRIADLTQPLE
HCHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
IIRTIHSFDPCIACSVHVMDFKGQSLNEFKVEPNFAKF
HHHHHHHCCCHHEEEEEEEECCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SKKIVVDPITRIEGHLRIEVIVDDNNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQR
CCCEEECCHHHCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGVTAVENALGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
LSALKADPIEASKLAFKYSPYPINTGAGELKAVQKRLNDFAKGGSLGPFSNGYYGHKTYR
HHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCHHHHHHH
KSKFEVVAHFINHAYYPDLVMAGEMFANEQSVIKGCGLKNFIAYEEVLLGRDKYLLSSGV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCC
VLDGDISKLHPINESLIKEEVTHSWYQYEDTKEVQLHPYDGQTNPHYTGFKDGESVGIEN
EEECCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
KMIPTKVLDTKNKYSWIKSPRYDNMPMEVGPLSAVVVGLAAKNPYITEVATKFLKDTKLP
CCCCHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCTPYEIDKNQEYKGRYIG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHH
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQGQRGAYEMSLIGTRIADLTQPLE
HCHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
IIRTIHSFDPCIACSVHVMDFKGQSLNEFKVEPNFAKF
HHHHHHHCCCHHEEEEEEEECCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]