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The map label for this gene is hyaA [H]

Identifier: 109947312

GI number: 109947312

Start: 652608

End: 653765

Strand: Direct

Name: hyaA [H]

Synonym: Hac_0744

Alternate gene names: 109947312

Gene position: 652608-653765 (Clockwise)

Preceding gene: 109947309

Following gene: 109947313

Centisome position: 42.0

GC content: 44.56

Gene sequence:

>1158_bases
ATGTTCTACGATGAAAAAAAGACCTATCAAAAGATTGAAGAACGCCTTGAGATAGTCAGATCGTTTAACGCTCATAACGA
GCATAAAAACTTGCAAGATGAGTTTAAGGGTGTGGGCATTTCTAGGCGTGATTTATTGAAGTGGGCGGGCATGATGAGCG
TGACTCTAGCTTTGCCGGTCAGTTTCGCTCCCTTGACTTTAAAGGCGGTGGAAGTGGCGAACAGATTGCCTGTGATTTGG
TTGCACATGGCAGAATGCACCGGCTGTAGCGAGAGCTTGTTAAGGAGTGCAGATCCCACTATTGATAGCATCATTTTTGA
TTATATCAACCTAGAATACCATGAGACTATCATGGTAGGGAGCGGTTTTCAAGCTGAAAAAAGCTTGCATGACGCCATAG
AAAAGCATAAAAATAATTACATTTTAATGGTAGAAGGCGGTATCCCACAAGGCACTGAATACTTTCTCACTCAAGGTCCA
AACGCTGAAACAGGGGCTGAAGAATGCAGAAGGGCTGCCAAATACGCAGCCGCTATTTTTGCCATAGGCACTTGCTCAAG
TTTTGGGGGCGTGCAAGCGGCTTACCCTAACCCTTCTAACGCACAACCCTTACACAAAATCATTGATAAACCTGTGATCA
ATGTGCCCGGTTGTCCGCCCAGTGAAAAAAATATTGTAGGCAATGTGCTTTATTACTTGATGTTTGGAGCTCTCCCTAAA
CTAGATGCGTATAACCGCCCCTCTTGGGCTTATGGGAATAGGATTCATGATTTGTGTGAAAGGAGAGGGCATTTTGATGC
GGGCGAATTTGTAGAACATTTTGGCGATGAAAACGCTAAAAAAGGGTTTTGCTTGTATAAAATGGGCTGTAAAGGGCCTT
ACACTTTCAATAATTGCTCCAAACTCCGCTTCAATTCACACACCTCTTGGCCCATAGGCGCAGGGCATGGGTGTATAGGG
TGTTCTGAGCCTAATTTTTGGGATACGATGAGCCCTTTTGAAGAGCCTTTAGCGAACCGCTCCATTAAAACCGCTTTTGA
TGGCTTGGGGGCTGATAAAGTGGCGGATAAAGTAGGCACGACTCTACTCAGTGCAGCCGCTATTGGCATTGCTGCACATG
CACTTCTTTCTAAAGCGATCAAAAATAAAGAGCAATAA

Upstream 100 bases:

>100_bases
TTTTTAATAATAGGGGGTATTCTATCCAAAATAAACCAATACTAAAGCCATTCTATTTTTATTGTGTTAAGATAATGCAA
ATTTTTATGAAGGAATGGCG

Downstream 100 bases:

>100_bases
GGGATTAAGATGTCAAAAAAAATAGTAGTTGATCCTATCACTAGGATTGAGGGGCATTTAAGGATTGAGGTTATCGTAGA
TGATAATAATGTGATTACTG

Product: hydrogenase small chain

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MFYDEKKTYQKIEERLEIVRSFNAHNEHKNLQDEFKGVGISRRDLLKWAGMMSVTLALPVSFAPLTLKAVEVANRLPVIW
LHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVGSGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGP
NAETGAEECRRAAKYAAAIFAIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKKGFCLYKMGCKGPYTFNNCSKLRFNSHTSWPIGAGHGCIG
CSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGTTLLSAAAIGIAAHALLSKAIKNKEQ

Sequences:

>Translated_385_residues
MFYDEKKTYQKIEERLEIVRSFNAHNEHKNLQDEFKGVGISRRDLLKWAGMMSVTLALPVSFAPLTLKAVEVANRLPVIW
LHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVGSGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGP
NAETGAEECRRAAKYAAAIFAIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKKGFCLYKMGCKGPYTFNNCSKLRFNSHTSWPIGAGHGCIG
CSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGTTLLSAAAIGIAAHALLSKAIKNKEQ
>Mature_385_residues
MFYDEKKTYQKIEERLEIVRSFNAHNEHKNLQDEFKGVGISRRDLLKWAGMMSVTLALPVSFAPLTLKAVEVANRLPVIW
LHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVGSGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGP
NAETGAEECRRAAKYAAAIFAIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKKGFCLYKMGCKGPYTFNNCSKLRFNSHTSWPIGAGHGCIG
CSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGTTLLSAAAIGIAAHALLSKAIKNKEQ

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=339, Percent_Identity=43.3628318584071, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1789371, Length=289, Percent_Identity=45.6747404844291, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 42509; Mature: 42509

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYDEKKTYQKIEERLEIVRSFNAHNEHKNLQDEFKGVGISRRDLLKWAGMMSVTLALPV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
SFAPLTLKAVEVANRLPVIWLHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVG
CCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEEC
SGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGPNAETGAEECRRAAKYAAAIF
CCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
HHHCCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKKGFCLYKMGCKGPYTFNNCS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCC
KLRFNSHTSWPIGAGHGCIGCSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGT
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
TLLSAAAIGIAAHALLSKAIKNKEQ
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFYDEKKTYQKIEERLEIVRSFNAHNEHKNLQDEFKGVGISRRDLLKWAGMMSVTLALPV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
SFAPLTLKAVEVANRLPVIWLHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVG
CCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEEC
SGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGPNAETGAEECRRAAKYAAAIF
CCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
HHHCCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKKGFCLYKMGCKGPYTFNNCS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCC
KLRFNSHTSWPIGAGHGCIGCSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGT
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
TLLSAAAIGIAAHALLSKAIKNKEQ
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]