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The map label for this gene is ispG [H]

Identifier: 109947305

GI number: 109947305

Start: 644616

End: 645692

Strand: Reverse

Name: ispG [H]

Synonym: Hac_0735

Alternate gene names: 109947305

Gene position: 645692-644616 (Counterclockwise)

Preceding gene: 109947310

Following gene: 109947304

Centisome position: 41.55

GC content: 40.67

Gene sequence:

>1077_bases
ATGCTAGAAAATAGAGTTAAGACCAAGCAAATTTTTATCGGTGGCGTGGCTATAGGGGGTGGTGCCCCCATAAGCACTCA
AAGCATGACCTTTAGTAAAACCGCTGATATTCAAAGCACTAAAAATCAAATTGACAGATTGAAACTCGCCGGGGCTGATT
TAGTGAGGGTGGCGGTGAGTAATGAAAAGGACGCTCTAGCCTTAAAAGAATTGAAAAAGGTGTCTCCTTTGCCTTTAATC
GCTGATATTCATTTCCATTATAAATTCGCTCTAATCGCTGCTCAGAGCGTTGATGCAATCAGGATCAATCCTGGAAACAT
CGGCTCTAAAGACAAAATTAAAGCGGTGGTTGATGCTTGTAAGGAAAAAAACATTCCTATAAGAATTGGCGTGAATGCTG
GGAGTTTAGAAAAGCAATTTGATCAAAAATACGGACCAACCGTTAAAGGCATGGTAGAAAGCGCTTTGTATAACGCTAAA
CTTTTAGAAGATTTGGATTTTACCAATTTTAAGATTTCTTTAAAAGCGAGCGATGTGATCCGCACCATAGAAGCTTATAG
AATGTTACGCCCCCTTGTAATCTATCCTTTCCATTTGGGGGTTACTGAAGCGGGTAATCTTTTTAGCTCCAGTATCAAAT
CCGCCATGGCTTTAGGGGGGCTTTTAATGGAGGGTATTGGAGACACGATGCGCATCTCTATCACAGGGGAATTAGAAAAT
GAAATCAAAGTAGCTAGAGCGATTTTACGCTATAGCGGGCGTTTGAAAGAGGGGATTAATTTGATTTCTTGCCCCACTTG
TGGGCGCATTGAAGCGAATTTAGTGGATATGGCGGGCAAGGTAGAAAAACGCTTAAGCCACATTAAAACCCCTTTAGACA
TTAGCGTGATGGGCTGTGTGGTGAATGCTTTAGGTGAAGCCAAACATGCCGACATGGCGATCGCTTTTGGGAATCGCAGT
GGTTTGATTATCAAAGAAGGTAAAGTCATTCACAAATTGGCCGAAAAGGATTTGTTTGAAACTTTTGTAACAGAAGTGGA
AAATTTAGCTAAAGAAAGAGAAAAAACTTTAAATTAA

Upstream 100 bases:

>100_bases
AAATTTAAGCGTTTCGTTTATTGCTGAAAGAACTAAAAAGAGTATAAGAGAGTATAAAAGAGTATAATAGCGAAAGAATT
TAACTTATTAAAGAGGTTTG

Downstream 100 bases:

>100_bases
AGGACAGGATGATGAATAAATTTAAAAATTTTGTGAGCAACTACCAGCAATCTGATTGCTATAAAGAGCCTTTAGGATTT
GGTATTGCTAGAGTGGATAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLENRVKTKQIFIGGVAIGGGAPISTQSMTFSKTADIQSTKNQIDRLKLAGADLVRVAVSNEKDALALKELKKVSPLPLI
ADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDACKEKNIPIRIGVNAGSLEKQFDQKYGPTVKGMVESALYNAK
LLEDLDFTNFKISLKASDVIRTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRISITGELEN
EIKVARAILRYSGRLKEGINLISCPTCGRIEANLVDMAGKVEKRLSHIKTPLDISVMGCVVNALGEAKHADMAIAFGNRS
GLIIKEGKVIHKLAEKDLFETFVTEVENLAKEREKTLN

Sequences:

>Translated_358_residues
MLENRVKTKQIFIGGVAIGGGAPISTQSMTFSKTADIQSTKNQIDRLKLAGADLVRVAVSNEKDALALKELKKVSPLPLI
ADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDACKEKNIPIRIGVNAGSLEKQFDQKYGPTVKGMVESALYNAK
LLEDLDFTNFKISLKASDVIRTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRISITGELEN
EIKVARAILRYSGRLKEGINLISCPTCGRIEANLVDMAGKVEKRLSHIKTPLDISVMGCVVNALGEAKHADMAIAFGNRS
GLIIKEGKVIHKLAEKDLFETFVTEVENLAKEREKTLN
>Mature_358_residues
MLENRVKTKQIFIGGVAIGGGAPISTQSMTFSKTADIQSTKNQIDRLKLAGADLVRVAVSNEKDALALKELKKVSPLPLI
ADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDACKEKNIPIRIGVNAGSLEKQFDQKYGPTVKGMVESALYNAK
LLEDLDFTNFKISLKASDVIRTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRISITGELEN
EIKVARAILRYSGRLKEGINLISCPTCGRIEANLVDMAGKVEKRLSHIKTPLDISVMGCVVNALGEAKHADMAIAFGNRS
GLIIKEGKVIHKLAEKDLFETFVTEVENLAKEREKTLN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=341, Percent_Identity=46.3343108504399, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 38992; Mature: 38992

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENRVKTKQIFIGGVAIGGGAPISTQSMTFSKTADIQSTKNQIDRLKLAGADLVRVAVS
CCCCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEC
NEKDALALKELKKVSPLPLIADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDAC
CCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
KEKNIPIRIGVNAGSLEKQFDQKYGPTVKGMVESALYNAKLLEDLDFTNFKISLKASDVI
HCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
RTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRISITGELEN
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
EIKVARAILRYSGRLKEGINLISCPTCGRIEANLVDMAGKVEKRLSHIKTPLDISVMGCV
HHHHHHHHHHHCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VNALGEAKHADMAIAFGNRSGLIIKEGKVIHKLAEKDLFETFVTEVENLAKEREKTLN
HHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLENRVKTKQIFIGGVAIGGGAPISTQSMTFSKTADIQSTKNQIDRLKLAGADLVRVAVS
CCCCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEC
NEKDALALKELKKVSPLPLIADIHFHYKFALIAAQSVDAIRINPGNIGSKDKIKAVVDAC
CCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
KEKNIPIRIGVNAGSLEKQFDQKYGPTVKGMVESALYNAKLLEDLDFTNFKISLKASDVI
HCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
RTIEAYRMLRPLVIYPFHLGVTEAGNLFSSSIKSAMALGGLLMEGIGDTMRISITGELEN
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
EIKVARAILRYSGRLKEGINLISCPTCGRIEANLVDMAGKVEKRLSHIKTPLDISVMGCV
HHHHHHHHHHHCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VNALGEAKHADMAIAFGNRSGLIIKEGKVIHKLAEKDLFETFVTEVENLAKEREKTLN
HHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA