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The map label for this gene is pheT [H]

Identifier: 109947062

GI number: 109947062

Start: 408124

End: 410415

Strand: Reverse

Name: pheT [H]

Synonym: Hac_0460

Alternate gene names: 109947062

Gene position: 410415-408124 (Counterclockwise)

Preceding gene: 109947063

Following gene: 109947061

Centisome position: 26.41

GC content: 36.26

Gene sequence:

>2292_bases
ATGAAACTAAGCGTTAATGATTTGAATGTTTTTGTCAATACGCCTAAAAATATAACCAAACTCTGTGAAAATTTGAGTTG
CTTAGGTTTAGAAGTGGAGGGCTGTGTTTCTTGTGTCGCTCCTAAAAATGTGGTTGTGGGTAAGGTTTTAGAAAAAGCCT
CTCATAAAAACGCCGAAAAACTCAGCGTGTGTCAAGTGGATATTGGCAAGGAAGTGTTGCAAATCGTGTGTGGGGCTAAA
AATGTTGCACAAGATCAATTCGTGCCAGTCGCTTTGAATGGGGCGATAATAAGCTCAACAACAATCGCTCCAACTGAGAT
TAGGGGGGTTGAAAGCCATGGAATGATTTGTTCTAGCGCTGAATTAGGCTTTCCTAAAATCAATGATGGTATTTTAGAAT
TAGATGAGAGTGTTGGGGAGTTGGTCGTAGGGAAAGAATTGAGTGAATACGCCCCTTTCAACACGCATGTTTTAGAAATT
TCATTAACCCCCAATCGTGGGGATTGCTTGAGTGTTTTAGGCGTTGCTAGAGAAATTAGCGCGTTTTATCGCGCGCCCCT
AAAACCTATTAAGGCTTTAAATTTTACGCAAAAAAGCGATTCAATCGTGCTTGATATTGATGAAAATATCCAGTCGCATT
TGGCTTATTATTTGATCCACAACCATTCTTTAAAAACCCCTTTAAACATCAAACTCTCACTCGCTCATAATAACGCCTTG
AGTGAGAATGATCTAAAAAATTTCATAGAATTTAGCATGCATTTTAGTGGGGTAATAATGAATATTTATTATTTGAACGC
TTATAGCCTAAATACCCCTATTGATTTAAGCGTCAAAAACGATGAAAACAACCTTGAAAGCGTTTATATTAATCATCAAA
AACTCTCCACTATCGCTATCAAGCATCAAAATCAAGACAATTTAAGCGAGTATTTGCTTTTAGAAGCGAGTTATACCGAT
CCTATTAGCCTGTCTTTAAGGTTACACGCCCTAAAAAACAAAACGCTTCAAAAAGACAACGCCCTTATTTATAGAAGCAC
TAGGGGGAGCAACCCTAATTTATTAGATGGCTTGAATTTTTTAAGCACCCATTTAAAAACCGCTATTTTGGAAAGCAAAC
AAACCAAGTATTCTTTAAAAAACCACGCCCTTACATTCAAAGTTGAAGACATTGCTGAAATTTTAGGGCTTGTTATAGAG
GAAGAAACAATTCAAAGCGTTTTAAAAAATTTAGGCTTTGAAGTCAAAATAAAAGGGTCAAATATAGAAGTTATAACGCC
CAACTTTAGGCATGATATTAAAACGATCCAAGATATTGCTGAAGAGATCTTACGCTTTTTAGGGATTGATAATTTGATTT
CTAAACCCCTTCATTGTGTTAGCAGTAAAAATTCAAACCCCCATTATGAAACGCACCGCTTTTTTGAAAACCTTAAACAC
AAGGCTCTTGCTTGCGGTTTTAAAGAAGTGATCCATTATGTGTTTTATTCTAAAGAAAAACAACAAAAATTGGGCTTTGA
GATTTTAGAAGATCCCCTAGAATTACAAAACCCTATCACAACGGAGTTAAACACTCTTAGAACGAGTCTTGTTTGCGGGC
TTTTAGACGCAAGCTTAAGGAATAAAAATTTAGGGTTTAAAAGCATAGCTTTGTATGAAAAGGGAAGCGTGTATAATTCT
AAAAGAGAAGAAATCCAAAAACTAGGCTTTTTAGCGAGCGGCTTACAAACTAAAGAAAGTTACCCTTATGCCAAAGGCAA
GGCGTGGGATTTTTACTCTTTTGCCGAATGCGTTTCAAAAATTGTAGGGGATTTTAGCTTGGAAAAACTAGACACTAAAA
CCCCCATTAACCACCCCTACCAGAGCGCTAAAATCATTCAAAATAATGAAGTAATAGGCGTAATCGCCAAAATCCACCCT
AAAGTTATCCAAGAATTGGATTTATTGGAAAGCTATTATGCTGAAATAGACGCTTCTAAACTCAAGCACCCTACCATGTT
ATTAAAACCCTTTAGCATTTACCCTAGCAGTGTGAGGGATTTGACTCTCATCATTGATGAGGATACCGCTTTTAATAAAA
TTAAAAAAGCTCTAAAAGACGCTCAAATCCCTAATTTAAGCGAAATTCTACCCCTTGATATTTTTAAAGAAAACAATAAC
GCAATAGCTTTAAGCGTGCGTTGCATGATCCATTCTTTAGAAAAAACCCTAAATGATGAAGAGGTCAATTTAGCCGTGCA
AAGAGTACTAGAAATTTTAGAAAAAAATTTTAACGTCCGCCTCAAAGGATAA

Upstream 100 bases:

>100_bases
CCTTTGGCATGGGGATTGAAAGATTAGCCATGCTAACTTGTCAAATCAATGATTTACGCAGTTTCTTTGAAACTGATTTA
AGGGTGTTGGAGGGTTTTTA

Downstream 100 bases:

>100_bases
GATAAAGGATAGTATTTGATAGAGCTTGATATTAACGCTAGCGACAAATCGCTCTCACACAGAGCCGTCATTTTTAGTCT
GCTTGCCAAAAAGCCTTGTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MKLSVNDLNVFVNTPKNITKLCENLSCLGLEVEGCVSCVAPKNVVVGKVLEKASHKNAEKLSVCQVDIGKEVLQIVCGAK
NVAQDQFVPVALNGAIISSTTIAPTEIRGVESHGMICSSAELGFPKINDGILELDESVGELVVGKELSEYAPFNTHVLEI
SLTPNRGDCLSVLGVAREISAFYRAPLKPIKALNFTQKSDSIVLDIDENIQSHLAYYLIHNHSLKTPLNIKLSLAHNNAL
SENDLKNFIEFSMHFSGVIMNIYYLNAYSLNTPIDLSVKNDENNLESVYINHQKLSTIAIKHQNQDNLSEYLLLEASYTD
PISLSLRLHALKNKTLQKDNALIYRSTRGSNPNLLDGLNFLSTHLKTAILESKQTKYSLKNHALTFKVEDIAEILGLVIE
EETIQSVLKNLGFEVKIKGSNIEVITPNFRHDIKTIQDIAEEILRFLGIDNLISKPLHCVSSKNSNPHYETHRFFENLKH
KALACGFKEVIHYVFYSKEKQQKLGFEILEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYNS
KREEIQKLGFLASGLQTKESYPYAKGKAWDFYSFAECVSKIVGDFSLEKLDTKTPINHPYQSAKIIQNNEVIGVIAKIHP
KVIQELDLLESYYAEIDASKLKHPTMLLKPFSIYPSSVRDLTLIIDEDTAFNKIKKALKDAQIPNLSEILPLDIFKENNN
AIALSVRCMIHSLEKTLNDEEVNLAVQRVLEILEKNFNVRLKG

Sequences:

>Translated_763_residues
MKLSVNDLNVFVNTPKNITKLCENLSCLGLEVEGCVSCVAPKNVVVGKVLEKASHKNAEKLSVCQVDIGKEVLQIVCGAK
NVAQDQFVPVALNGAIISSTTIAPTEIRGVESHGMICSSAELGFPKINDGILELDESVGELVVGKELSEYAPFNTHVLEI
SLTPNRGDCLSVLGVAREISAFYRAPLKPIKALNFTQKSDSIVLDIDENIQSHLAYYLIHNHSLKTPLNIKLSLAHNNAL
SENDLKNFIEFSMHFSGVIMNIYYLNAYSLNTPIDLSVKNDENNLESVYINHQKLSTIAIKHQNQDNLSEYLLLEASYTD
PISLSLRLHALKNKTLQKDNALIYRSTRGSNPNLLDGLNFLSTHLKTAILESKQTKYSLKNHALTFKVEDIAEILGLVIE
EETIQSVLKNLGFEVKIKGSNIEVITPNFRHDIKTIQDIAEEILRFLGIDNLISKPLHCVSSKNSNPHYETHRFFENLKH
KALACGFKEVIHYVFYSKEKQQKLGFEILEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYNS
KREEIQKLGFLASGLQTKESYPYAKGKAWDFYSFAECVSKIVGDFSLEKLDTKTPINHPYQSAKIIQNNEVIGVIAKIHP
KVIQELDLLESYYAEIDASKLKHPTMLLKPFSIYPSSVRDLTLIIDEDTAFNKIKKALKDAQIPNLSEILPLDIFKENNN
AIALSVRCMIHSLEKTLNDEEVNLAVQRVLEILEKNFNVRLKG
>Mature_763_residues
MKLSVNDLNVFVNTPKNITKLCENLSCLGLEVEGCVSCVAPKNVVVGKVLEKASHKNAEKLSVCQVDIGKEVLQIVCGAK
NVAQDQFVPVALNGAIISSTTIAPTEIRGVESHGMICSSAELGFPKINDGILELDESVGELVVGKELSEYAPFNTHVLEI
SLTPNRGDCLSVLGVAREISAFYRAPLKPIKALNFTQKSDSIVLDIDENIQSHLAYYLIHNHSLKTPLNIKLSLAHNNAL
SENDLKNFIEFSMHFSGVIMNIYYLNAYSLNTPIDLSVKNDENNLESVYINHQKLSTIAIKHQNQDNLSEYLLLEASYTD
PISLSLRLHALKNKTLQKDNALIYRSTRGSNPNLLDGLNFLSTHLKTAILESKQTKYSLKNHALTFKVEDIAEILGLVIE
EETIQSVLKNLGFEVKIKGSNIEVITPNFRHDIKTIQDIAEEILRFLGIDNLISKPLHCVSSKNSNPHYETHRFFENLKH
KALACGFKEVIHYVFYSKEKQQKLGFEILEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYNS
KREEIQKLGFLASGLQTKESYPYAKGKAWDFYSFAECVSKIVGDFSLEKLDTKTPINHPYQSAKIIQNNEVIGVIAKIHP
KVIQELDLLESYYAEIDASKLKHPTMLLKPFSIYPSSVRDLTLIIDEDTAFNKIKKALKDAQIPNLSEILPLDIFKENNN
AIALSVRCMIHSLEKTLNDEEVNLAVQRVLEILEKNFNVRLKG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=814, Percent_Identity=25.7985257985258, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85460; Mature: 85460

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSVNDLNVFVNTPKNITKLCENLSCLGLEVEGCVSCVAPKNVVVGKVLEKASHKNAEK
CEEEEEEEEEEEECCHHHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
LSVCQVDIGKEVLQIVCGAKNVAQDQFVPVALNGAIISSTTIAPTEIRGVESHGMICSSA
EEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCHHCCCCCCCCEEEECC
ELGFPKINDGILELDESVGELVVGKELSEYAPFNTHVLEISLTPNRGDCLSVLGVAREIS
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AFYRAPLKPIKALNFTQKSDSIVLDIDENIQSHLAYYLIHNHSLKTPLNIKLSLAHNNAL
HHHHCCCCHHHHCCCCCCCCCEEEEECCCHHHHHEEEEEECCCCCCCCEEEEEEECCCCC
SENDLKNFIEFSMHFSGVIMNIYYLNAYSLNTPIDLSVKNDENNLESVYINHQKLSTIAI
CHHHHHHHHHHHHHHCEEEEEEEEEEEEECCCCEEEEEECCCCCCEEEEEECCEEEEEEE
KHQNQDNLSEYLLLEASYTDPISLSLRLHALKNKTLQKDNALIYRSTRGSNPNLLDGLNF
ECCCCCCCCCEEEEEECCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHH
LSTHLKTAILESKQTKYSLKNHALTFKVEDIAEILGLVIEEETIQSVLKNLGFEVKIKGS
HHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHCCEEEEEECC
NIEVITPNFRHDIKTIQDIAEEILRFLGIDNLISKPLHCVSSKNSNPHYETHRFFENLKH
EEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KALACGFKEVIHYVFYSKEKQQKLGFEILEDPLELQNPITTELNTLRTSLVCGLLDASLR
HHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHC
NKNLGFKSIALYEKGSVYNSKREEIQKLGFLASGLQTKESYPYAKGKAWDFYSFAECVSK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
IVGDFSLEKLDTKTPINHPYQSAKIIQNNEVIGVIAKIHPKVIQELDLLESYYAEIDASK
HHCCCCHHHCCCCCCCCCCCCCCEEEECCCEEEEHHHHCHHHHHHHHHHHHHHHHCCHHH
LKHPTMLLKPFSIYPSSVRDLTLIIDEDTAFNKIKKALKDAQIPNLSEILPLDIFKENNN
CCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHCCEEEEECCCC
AIALSVRCMIHSLEKTLNDEEVNLAVQRVLEILEKNFNVRLKG
EEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MKLSVNDLNVFVNTPKNITKLCENLSCLGLEVEGCVSCVAPKNVVVGKVLEKASHKNAEK
CEEEEEEEEEEEECCHHHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
LSVCQVDIGKEVLQIVCGAKNVAQDQFVPVALNGAIISSTTIAPTEIRGVESHGMICSSA
EEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCHHCCCCCCCCEEEECC
ELGFPKINDGILELDESVGELVVGKELSEYAPFNTHVLEISLTPNRGDCLSVLGVAREIS
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AFYRAPLKPIKALNFTQKSDSIVLDIDENIQSHLAYYLIHNHSLKTPLNIKLSLAHNNAL
HHHHCCCCHHHHCCCCCCCCCEEEEECCCHHHHHEEEEEECCCCCCCCEEEEEEECCCCC
SENDLKNFIEFSMHFSGVIMNIYYLNAYSLNTPIDLSVKNDENNLESVYINHQKLSTIAI
CHHHHHHHHHHHHHHCEEEEEEEEEEEEECCCCEEEEEECCCCCCEEEEEECCEEEEEEE
KHQNQDNLSEYLLLEASYTDPISLSLRLHALKNKTLQKDNALIYRSTRGSNPNLLDGLNF
ECCCCCCCCCEEEEEECCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHH
LSTHLKTAILESKQTKYSLKNHALTFKVEDIAEILGLVIEEETIQSVLKNLGFEVKIKGS
HHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHCCEEEEEECC
NIEVITPNFRHDIKTIQDIAEEILRFLGIDNLISKPLHCVSSKNSNPHYETHRFFENLKH
EEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KALACGFKEVIHYVFYSKEKQQKLGFEILEDPLELQNPITTELNTLRTSLVCGLLDASLR
HHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHC
NKNLGFKSIALYEKGSVYNSKREEIQKLGFLASGLQTKESYPYAKGKAWDFYSFAECVSK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
IVGDFSLEKLDTKTPINHPYQSAKIIQNNEVIGVIAKIHPKVIQELDLLESYYAEIDASK
HHCCCCHHHCCCCCCCCCCCCCCEEEECCCEEEEHHHHCHHHHHHHHHHHHHHHHCCHHH
LKHPTMLLKPFSIYPSSVRDLTLIIDEDTAFNKIKKALKDAQIPNLSEILPLDIFKENNN
CCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHCCEEEEECCCC
AIALSVRCMIHSLEKTLNDEEVNLAVQRVLEILEKNFNVRLKG
EEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]