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The map label for this gene is pheS

Identifier: 109947063

GI number: 109947063

Start: 410415

End: 411401

Strand: Reverse

Name: pheS

Synonym: Hac_0461

Alternate gene names: 109947063

Gene position: 411401-410415 (Counterclockwise)

Preceding gene: 109947071

Following gene: 109947062

Centisome position: 26.47

GC content: 39.01

Gene sequence:

>987_bases
TTGCACACCTTAATAGAGCGATTAGAAAAGGTTACTAATAACAAAGAATTAGAAGAAGTGCGCTTGAATGCTTTGGGTAA
AAAAGGGGTTTTTGCGGATAAATTCAACCAACTTAAAAATCTAAACGGCGGAGAAAAAAACGCCTTTGCTAAAGAAATCC
ACCATTATAAACAAGCGTTTGAAAAAGCCTTTGAATTGAAAAAAAAGGCTATCATAGAGCTTGAGTTAGAAGAACGCTTG
AAAAAAGAAAAAATTGATGTGAGTTTGTTTAACACTACCAAAACAAGCTCTTCTCACCCTTTAAATTACACTAAAAATAA
AATCATTGAATTTTTTACCCCTTTAGGATACAAGCTTGAAATCGGCTCTTTAGTGGAAGATGATTTTCATAATTTTAGCG
CTTTAAACTTGCCCCCTTACCATCCGGCAAGAGATATGCAAGATACTTTCTACTTTAAAGATCACAAACTTTTAAGGACC
CATACTTCGCCTGTGCAAATCCACACCATGCAAGAACAAACCCCCCCCATTAAAATGATTTGTTTAGGCGAAACCTTTAG
ACGCGATTATGATTTGACCCACACGCCCATGTTCCATCAAATTGAAGGGCTTGTCGTGGATCAAAAAGGGAATATCCATT
TCACGCATTTAAAGGGCGTGATAGAAGACTTTTTGCATTATTTCTTTGGGGGCGTGAAATTAAGGTGGCGCTCTAGCTTT
TTCCCTTTCACAGAGCCAAGTGCTGAAGTGGATATTAGCTGCGTGTTTTGCAAGCAAGAAGGCTGTAGGGTTTGCTCGCA
TATGGGCTGGCTAGAAGTGTTAGGCTGTGGCATGGTCAATAATGCGGTGTTTGAAGCCATAGGGTATAAGGGCATGAGTG
GGTTTGCCTTTGGCATGGGGATTGAAAGATTAGCCATGCTAACTTGTCAAATCAATGATTTACGCAGTTTCTTTGAAACT
GATTTAAGGGTGTTGGAGGGTTTTTAA

Upstream 100 bases:

>100_bases
ATCTTTTCAAACACATTCATATGCTTTTTGCTCCCTTTTAAGGTTTTTCTTTTATTAAAGTTGTATTATAGCTTTTTAAA
ATAAAATATAAGGATAGGGA

Downstream 100 bases:

>100_bases
TGAAACTAAGCGTTAATGATTTGAATGTTTTTGTCAATACGCCTAAAAATATAACCAAACTCTGTGAAAATTTGAGTTGC
TTAGGTTTAGAAGTGGAGGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MHTLIERLEKVTNNKELEEVRLNALGKKGVFADKFNQLKNLNGGEKNAFAKEIHHYKQAFEKAFELKKKAIIELELEERL
KKEKIDVSLFNTTKTSSSHPLNYTKNKIIEFFTPLGYKLEIGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRT
HTSPVQIHTMQEQTPPIKMICLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIHFTHLKGVIEDFLHYFFGGVKLRWRSSF
FPFTEPSAEVDISCVFCKQEGCRVCSHMGWLEVLGCGMVNNAVFEAIGYKGMSGFAFGMGIERLAMLTCQINDLRSFFET
DLRVLEGF

Sequences:

>Translated_328_residues
MHTLIERLEKVTNNKELEEVRLNALGKKGVFADKFNQLKNLNGGEKNAFAKEIHHYKQAFEKAFELKKKAIIELELEERL
KKEKIDVSLFNTTKTSSSHPLNYTKNKIIEFFTPLGYKLEIGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRT
HTSPVQIHTMQEQTPPIKMICLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIHFTHLKGVIEDFLHYFFGGVKLRWRSSF
FPFTEPSAEVDISCVFCKQEGCRVCSHMGWLEVLGCGMVNNAVFEAIGYKGMSGFAFGMGIERLAMLTCQINDLRSFFET
DLRVLEGF
>Mature_328_residues
MHTLIERLEKVTNNKELEEVRLNALGKKGVFADKFNQLKNLNGGEKNAFAKEIHHYKQAFEKAFELKKKAIIELELEERL
KKEKIDVSLFNTTKTSSSHPLNYTKNKIIEFFTPLGYKLEIGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRT
HTSPVQIHTMQEQTPPIKMICLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIHFTHLKGVIEDFLHYFFGGVKLRWRSSF
FPFTEPSAEVDISCVFCKQEGCRVCSHMGWLEVLGCGMVNNAVFEAIGYKGMSGFAFGMGIERLAMLTCQINDLRSFFET
DLRVLEGF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=29.5620437956204, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4758340, Length=266, Percent_Identity=27.8195488721804, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=46.1538461538462, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32566635, Length=283, Percent_Identity=30.3886925795053, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321087, Length=266, Percent_Identity=27.8195488721804, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325304, Length=313, Percent_Identity=26.517571884984, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=27.3356401384083, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_HELAH (Q17YI4)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664291.1
- ProteinModelPortal:   Q17YI4
- SMR:   Q17YI4
- STRING:   Q17YI4
- GeneID:   4176778
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0461
- NMPDR:   fig|382638.8.peg.450
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   HACI382638:HAC_0461-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37905; Mature: 37905

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTLIERLEKVTNNKELEEVRLNALGKKGVFADKFNQLKNLNGGEKNAFAKEIHHYKQAF
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EKAFELKKKAIIELELEERLKKEKIDVSLFNTTKTSSSHPLNYTKNKIIEFFTPLGYKLE
HHHHHHHHHHEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEE
IGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRTHTSPVQIHTMQEQTPPIKMI
ECCHHHHHHCCCEECCCCCCCCCCCCCHHHEECCCHHEEECCCCEEEEEECCCCCCEEEE
CLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIHFTHLKGVIEDFLHYFFGGVKLRWRSSF
ECCHHHHCCCCCCCCCHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEEEECCC
FPFTEPSAEVDISCVFCKQEGCRVCSHMGWLEVLGCGMVNNAVFEAIGYKGMSGFAFGMG
CCCCCCCCCEEEEEEEECCHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
IERLAMLTCQINDLRSFFETDLRVLEGF
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHTLIERLEKVTNNKELEEVRLNALGKKGVFADKFNQLKNLNGGEKNAFAKEIHHYKQAF
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EKAFELKKKAIIELELEERLKKEKIDVSLFNTTKTSSSHPLNYTKNKIIEFFTPLGYKLE
HHHHHHHHHHEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEE
IGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRTHTSPVQIHTMQEQTPPIKMI
ECCHHHHHHCCCEECCCCCCCCCCCCCHHHEECCCHHEEECCCCEEEEEECCCCCCEEEE
CLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIHFTHLKGVIEDFLHYFFGGVKLRWRSSF
ECCHHHHCCCCCCCCCHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEEEECCC
FPFTEPSAEVDISCVFCKQEGCRVCSHMGWLEVLGCGMVNNAVFEAIGYKGMSGFAFGMG
CCCCCCCCCEEEEEEEECCHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
IERLAMLTCQINDLRSFFETDLRVLEGF
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA