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The map label for this gene is mod fragment 1 [H]

Identifier: 109946809

GI number: 109946809

Start: 156268

End: 156540

Strand: Direct

Name: mod fragment 1 [H]

Synonym: Hac_0186

Alternate gene names: 109946809

Gene position: 156268-156540 (Clockwise)

Preceding gene: 109946808

Following gene: 109946810

Centisome position: 10.06

GC content: 28.21

Gene sequence:

>273_bases
TTGAATAAGATTTATATAGAAGATGTTTTTGCTTTCTTAGACAAGTTAGAAGATAAAAGCGTTGATTTAGCTATTATTGA
CCCTCCTTACAATCTTAAAATTGCTTCATGGGATAGTTTTAAAAATGATGAAGAATTTTTAACATTTTCTTACGCTTGGA
TTGATAAAATGCTACCCAAAATCAAAGACACAGGGAGTTTTTATATCTTTAATACCCCCTTTAACTGCGCTTTATTTTTA
GCATATTTGTGTCAAAAAAAGTGCATTTTTTAA

Upstream 100 bases:

>100_bases
TTAAGAGAAGCAAAACTTTTAAAAAGGAATTTTATAGGCTATGAACTCTATGAAAATTATAAGCCCTTGATTGAGCAAAA
ATTAGGAAACTTGTTTGATT

Downstream 100 bases:

>100_bases
ATTTTATCACTTGGGTCAAAAAAGATGGGTTTGCCAACGCTAAAAAGCGTTATAACCATGCACAAGAAAGCATTTTATTT
TATAGCATGCATAAGAAAAA

Product: type IIS restriction enzyme M1 protein fragment 1

Products: NA

Alternate protein names: M.MboII; Adenine-specific methyltransferase MboII; DNA MTase MboIIA [H]

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKIYIEDVFAFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPKIKDTGSFYIFNTPFNCALFL
AYLCQKKCIF

Sequences:

>Translated_90_residues
MNKIYIEDVFAFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPKIKDTGSFYIFNTPFNCALFL
AYLCQKKCIF
>Mature_90_residues
MNKIYIEDVFAFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPKIKDTGSFYIFNTPFNCALFL
AYLCQKKCIF

Specific function: This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 10628; Mature: 10628

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIYIEDVFAFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPK
CCEEHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHH
IKDTGSFYIFNTPFNCALFLAYLCQKKCIF
CCCCCCEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKIYIEDVFAFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPK
CCEEHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHH
IKDTGSFYIFNTPFNCALFLAYLCQKKCIF
CCCCCCEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2020540 [H]