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The map label for this gene is mjaIM [H]

Identifier: 109946808

GI number: 109946808

Start: 155403

End: 156275

Strand: Direct

Name: mjaIM [H]

Synonym: Hac_0185

Alternate gene names: 109946808

Gene position: 155403-156275 (Clockwise)

Preceding gene: 109946801

Following gene: 109946809

Centisome position: 10.0

GC content: 34.71

Gene sequence:

>873_bases
TTGAACATCAATAAAGTGTTTTATCATAGTAGCACCAACATGAATGAAGTGCTAGATAATAGCGTGGATTTAATCATCAC
AAGCCCACCTTATTTCAACATTAAAGATTACACGAAAAATGGCACACAAGATTTACAGCATTCAACCCAACATGTTGAAG
ATTTAGGGGCGTTAGAAAAATATGAAGATTACCTTTTAGGTCTTTTAAAAGTTTGGCATGAATGCTATAGGGTGCTAAAA
CCAAATGGTAAGCTATGCATTAATGTGCCTTTAATACCCATGCTTAAAAAGGTTTTAAACACGCACTATAACCGCCATAT
TTTTGATCTGCATGCTGATATTCAACACTCCATTTTGCGTGATTTAAACAACATGTTGGAAAACAAGCCTAAAATATTTT
TATTAGATGTCTATATTTGGAAACGCACAAATCCTACTAAAAAATTGATGTTTGGGAGTTACCCTTATCCTAGAAATTTT
TATGCACAAAATACTATAGAATTTATTGGCGTGTTTGTCAAAGACGGCAAGCCCAAACAACCCACAAAAGAACAAAGAGA
ACAAAAAGAACAAAGCCAATTAACTCAAGAGGAGTGGGTAGAATTTACCAAACAAATTTGGGAAATCCCAATCCCTAACA
AAAACGATATTGCTTTTGGTAAGCATGCGGCTTTAATGCCGGCTGAATTAGCAAGGCGTTTGATTAGATTGTATAGTTGC
GTGGGCGATGTGGTGCTAGATCCATTTAGCGGGAGCGGAACAACTTTAAGAGAAGCAAAACTTTTAAAAAGGAATTTTAT
AGGCTATGAACTCTATGAAAATTATAAGCCCTTGATTGAGCAAAAATTAGGAAACTTGTTTGATTTTGAATAA

Upstream 100 bases:

>100_bases
CTCTAATTTTTTATAGATCCATGCTTTAAAAGGCTCTCATTTCATTGTAAAATTCTTTCATGTAAATCTATTAAGTTCTT
AATAAAAGGTAAAAATAACA

Downstream 100 bases:

>100_bases
GATTTATATAGAAGATGTTTTTGCTTTCTTAGACAAGTTAGAAGATAAAAGCGTTGATTTAGCTATTATTGACCCTCCTT
ACAATCTTAAAATTGCTTCA

Product: modification methylase mjai

Products: NA

Alternate protein names: M.MjaI; N-4 cytosine-specific methyltransferase MjaI [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNINKVFYHSSTNMNEVLDNSVDLIITSPPYFNIKDYTKNGTQDLQHSTQHVEDLGALEKYEDYLLGLLKVWHECYRVLK
PNGKLCINVPLIPMLKKVLNTHYNRHIFDLHADIQHSILRDLNNMLENKPKIFLLDVYIWKRTNPTKKLMFGSYPYPRNF
YAQNTIEFIGVFVKDGKPKQPTKEQREQKEQSQLTQEEWVEFTKQIWEIPIPNKNDIAFGKHAALMPAELARRLIRLYSC
VGDVVLDPFSGSGTTLREAKLLKRNFIGYELYENYKPLIEQKLGNLFDFE

Sequences:

>Translated_290_residues
MNINKVFYHSSTNMNEVLDNSVDLIITSPPYFNIKDYTKNGTQDLQHSTQHVEDLGALEKYEDYLLGLLKVWHECYRVLK
PNGKLCINVPLIPMLKKVLNTHYNRHIFDLHADIQHSILRDLNNMLENKPKIFLLDVYIWKRTNPTKKLMFGSYPYPRNF
YAQNTIEFIGVFVKDGKPKQPTKEQREQKEQSQLTQEEWVEFTKQIWEIPIPNKNDIAFGKHAALMPAELARRLIRLYSC
VGDVVLDPFSGSGTTLREAKLLKRNFIGYELYENYKPLIEQKLGNLFDFE
>Mature_290_residues
MNINKVFYHSSTNMNEVLDNSVDLIITSPPYFNIKDYTKNGTQDLQHSTQHVEDLGALEKYEDYLLGLLKVWHECYRVLK
PNGKLCINVPLIPMLKKVLNTHYNRHIFDLHADIQHSILRDLNNMLENKPKIFLLDVYIWKRTNPTKKLMFGSYPYPRNF
YAQNTIEFIGVFVKDGKPKQPTKEQREQKEQSQLTQEEWVEFTKQIWEIPIPNKNDIAFGKHAALMPAELARRLIRLYSC
VGDVVLDPFSGSGTTLREAKLLKRNFIGYELYENYKPLIEQKLGNLFDFE

Specific function: This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 34044; Mature: 34044

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINKVFYHSSTNMNEVLDNSVDLIITSPPYFNIKDYTKNGTQDLQHSTQHVEDLGALEK
CCCCEEEEECCCCHHHHHCCCCCEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHH
YEDYLLGLLKVWHECYRVLKPNGKLCINVPLIPMLKKVLNTHYNRHIFDLHADIQHSILR
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
DLNNMLENKPKIFLLDVYIWKRTNPTKKLMFGSYPYPRNFYAQNTIEFIGVFVKDGKPKQ
HHHHHHHCCCCEEEEEEEEEECCCCHHHHEECCCCCCCCCCHHHHHHHHEEEEECCCCCC
PTKEQREQKEQSQLTQEEWVEFTKQIWEIPIPNKNDIAFGKHAALMPAELARRLIRLYSC
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
VGDVVLDPFSGSGTTLREAKLLKRNFIGYELYENYKPLIEQKLGNLFDFE
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNINKVFYHSSTNMNEVLDNSVDLIITSPPYFNIKDYTKNGTQDLQHSTQHVEDLGALEK
CCCCEEEEECCCCHHHHHCCCCCEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHH
YEDYLLGLLKVWHECYRVLKPNGKLCINVPLIPMLKKVLNTHYNRHIFDLHADIQHSILR
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
DLNNMLENKPKIFLLDVYIWKRTNPTKKLMFGSYPYPRNFYAQNTIEFIGVFVKDGKPKQ
HHHHHHHCCCCEEEEEEEEEECCCCHHHHEECCCCCCCCCCHHHHHHHHEEEEECCCCCC
PTKEQREQKEQSQLTQEEWVEFTKQIWEIPIPNKNDIAFGKHAALMPAELARRLIRLYSC
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
VGDVVLDPFSGSGTTLREAKLLKRNFIGYELYENYKPLIEQKLGNLFDFE
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]