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The map label for this gene is nusA [H]

Identifier: 109946702

GI number: 109946702

Start: 67196

End: 68383

Strand: Reverse

Name: nusA [H]

Synonym: Hac_0069

Alternate gene names: 109946702

Gene position: 68383-67196 (Counterclockwise)

Preceding gene: 109946703

Following gene: 109946693

Centisome position: 4.4

GC content: 37.79

Gene sequence:

>1188_bases
ATGGAAAAAATCAGCGACCTTATAGAATGCATTGCGTATGAAAAAAATTTGCCTAAAGAGATGATTTCAAAAGTGGTTCA
AGGTTGTTTGTTAAAAATGGCGCAAAATGAATTAGACCCCCTAGCACGCTACTTAGTGATTGAAGAAAACAAGCAGCTCC
AGCTTATCCAGTTGGTAGAAGTTTTAGAAGATGATGATGAAGGATTGATTAACGATCCTTCTAAATATATTAGCCTCTCT
AAAGCCAAAGAAATGGATCCGAGCGTTAAGATTAAAGATGAATTGTCTTATAGTTTGAGTTTAGAGAGCATGAAACAAGG
AGCGATCAACCGCCTTTTTAAAGATTTGCAATACCAGTTAGAAAAGGCGTTAGAAGACAGCCATTTTGAAGCGTTCCAAA
AGCGTCTCAACAGCGTTTTAATGGGGCAAGTGATTTTAGTGGATAACAATCAAAACACTTTTATTGAAATTGAGCAACAA
TTTCAAGGCGTTCTTTCCATGCGCCATCGCATCAAGGGCGAGAGTTTTAAAATAGGCGATAGCATTAAGGCGGTTTTAAC
GCAAGTCAAACGCACTAAAAAAGGCTTGTTATTAGAGCTTAGCCGCACCACCCCTAAAATGCTTGAAGCTTTGCTAGAAT
TAGAAGTCCCTGAGATTAAGGATAAAGAAATTGAAATTATCCATTGCGTGCGAATCCCAGGTAATAGAGCGAAAGTGAGC
TTTTTTTCTAACAATTCTAGGATTGATCCCATAGGTGCGGCTGTAGGGGTTAAAGGCGTGCGCATTAATGCGATAAGCAA
TGAATTGAATAAAGAAAATATTGATTGCATAGAGTATTCTAATGTGCCTGAAATTTATATCACTTTGGCGCTCGCTCCTG
CTAAAATTTTAAGCGTTGAGATTAAAAAAATTCCCATAGAAGAATTGAGCGCTCAAGAAAAAGAGTTAATCCAAGAGCGT
TTTATTGTCAATAACCATTTGCAAAAGGCTAAAGTGCGTTTGTTAGACACGGAAAAATCTAAGGCTATCGGCAAGGGTGG
GGTGAATGTGTGCCTAGCGTCCATGCTTACTAGCTATCATATAGAGTTTGAAACCATTCCTAGCGTCAAAGAAAATACAG
AAAATGAAGACGAAAAAGAAACACCAAAAGTAGGGGTAGAAGCTTTAGAGTCTTTGTTTAAGAATTAA

Upstream 100 bases:

>100_bases
CTCTAGTTTTAGAAGAAATCCTTAATAAGGCTTAAAAATTCGCTATAATAGAATCTCTTTTAAAATACTCTCTCCTACAA
AAATTTAAAGGAACTCAAAA

Downstream 100 bases:

>100_bases
AGACAAGCTCTAAAAAGAGCTTTAAGTTGAAAAACTTGCGGTGTTAAGGGTGTATGACTAATTTTTTTAAGGCTAAGTTT
GAGCCTATTTTTCACTAAAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MEKISDLIECIAYEKNLPKEMISKVVQGCLLKMAQNELDPLARYLVIEENKQLQLIQLVEVLEDDDEGLINDPSKYISLS
KAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQLEKALEDSHFEAFQKRLNSVLMGQVILVDNNQNTFIEIEQQ
FQGVLSMRHRIKGESFKIGDSIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCVRIPGNRAKVS
FFSNNSRIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVEIKKIPIEELSAQEKELIQER
FIVNNHLQKAKVRLLDTEKSKAIGKGGVNVCLASMLTSYHIEFETIPSVKENTENEDEKETPKVGVEALESLFKN

Sequences:

>Translated_395_residues
MEKISDLIECIAYEKNLPKEMISKVVQGCLLKMAQNELDPLARYLVIEENKQLQLIQLVEVLEDDDEGLINDPSKYISLS
KAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQLEKALEDSHFEAFQKRLNSVLMGQVILVDNNQNTFIEIEQQ
FQGVLSMRHRIKGESFKIGDSIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCVRIPGNRAKVS
FFSNNSRIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVEIKKIPIEELSAQEKELIQER
FIVNNHLQKAKVRLLDTEKSKAIGKGGVNVCLASMLTSYHIEFETIPSVKENTENEDEKETPKVGVEALESLFKN
>Mature_395_residues
MEKISDLIECIAYEKNLPKEMISKVVQGCLLKMAQNELDPLARYLVIEENKQLQLIQLVEVLEDDDEGLINDPSKYISLS
KAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQLEKALEDSHFEAFQKRLNSVLMGQVILVDNNQNTFIEIEQQ
FQGVLSMRHRIKGESFKIGDSIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCVRIPGNRAKVS
FFSNNSRIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVEIKKIPIEELSAQEKELIQER
FIVNNHLQKAKVRLLDTEKSKAIGKGGVNVCLASMLTSYHIEFETIPSVKENTENEDEKETPKVGVEALESLFKN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=390, Percent_Identity=24.8717948717949, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 44709; Mature: 44709

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISDLIECIAYEKNLPKEMISKVVQGCLLKMAQNELDPLARYLVIEENKQLQLIQLVE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
VLEDDDEGLINDPSKYISLSKAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQL
HHCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCHHEEHHHHHHHHHHHHHHHHHHHH
EKALEDSHFEAFQKRLNSVLMGQVILVDNNQNTFIEIEQQFQGVLSMRHRIKGESFKIGD
HHHHCHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEECCH
SIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCVRIPGNRAKVS
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEE
FFSNNSRIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVE
EECCCCCCCCCHHHHCCCCEEEEEHHHHCCCCCCCEEECCCCCEEEEEEEECCHHHHEEH
IKKIPIEELSAQEKELIQERFIVNNHLQKAKVRLLDTEKSKAIGKGGVNVCLASMLTSYH
HHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHCCCCHHHHHHHHHHHHHC
IEFETIPSVKENTENEDEKETPKVGVEALESLFKN
EEEEECCCHHCCCCCCCHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKISDLIECIAYEKNLPKEMISKVVQGCLLKMAQNELDPLARYLVIEENKQLQLIQLVE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
VLEDDDEGLINDPSKYISLSKAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQL
HHCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCHHEEHHHHHHHHHHHHHHHHHHHH
EKALEDSHFEAFQKRLNSVLMGQVILVDNNQNTFIEIEQQFQGVLSMRHRIKGESFKIGD
HHHHCHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEECCH
SIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCVRIPGNRAKVS
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEE
FFSNNSRIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVE
EECCCCCCCCCHHHHCCCCEEEEEHHHHCCCCCCCEEECCCCCEEEEEEEECCHHHHEEH
IKKIPIEELSAQEKELIQERFIVNNHLQKAKVRLLDTEKSKAIGKGGVNVCLASMLTSYH
HHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHCCCCHHHHHHHHHHHHHC
IEFETIPSVKENTENEDEKETPKVGVEALESLFKN
EEEEECCCHHCCCCCCCHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]