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The map label for this gene is tgt [H]

Identifier: 108801911

GI number: 108801911

Start: 5239251

End: 5240483

Strand: Reverse

Name: tgt [H]

Synonym: Mmcs_4948

Alternate gene names: 108801911

Gene position: 5240483-5239251 (Counterclockwise)

Preceding gene: 108801912

Following gene: 108801910

Centisome position: 91.85

GC content: 68.53

Gene sequence:

>1233_bases
GTGGATCAGCAGTTCTTCACCGTCGAGGCCGAGCTGCCCGGGCGACGCGGCCGGGCCGGGGTGATCCGCACCCCGCACGG
GGAGATCCGCACCCCCGCGTTCATCGCCGTCGGCACCCAGGCCACCGTCAAGGCGGTGCTACCGGAGACCATGCGCGAGC
TCGGCGCCCAGGCGGTACTCGCCAACGCCTACCACCTCTATCTGCAACCCGGCCCGGACGTGGTCGCTGCCGCGGGCGGG
CTCGGCGCCTTCATGAACTGGCCCGGCCCCACGTTCACCGACAGCGGCGGGTTCCAGGTCCTGTCGCTGGGTGCCGGGTT
CCGCAAGGTGCTGGCGATGGACGCCAACAGAGTGCAGGCCGACGACGTCATCGCCAAGGGCAAGGAACGCCTGGCCGTGG
TCGACGACGACGGCGTCACCTTCCGGTCGCACCTCAACGGCACCACCCACAGATTCACCCCGGAGGTGTCGATCGGCATC
CAGCACCAACTGGGCGCCGACATCATCTTCGCCTTCGACGAGTTGACGACCCTGGTCAACACGCGCGGCTACCAGGAGGA
TTCGGTCCGCCGCACCCACCGCTGGGCGGAGCGCTGTCTGGCCGAGCACCGCAAGCTGTCGCTGGAGAACCACGAGAAGC
CGCCGCAGGCGCTGTTCGGTGTGGTCCAGGGCGCGCAGTACGAAGACCTCCGGCGGCAGGCCGCCCGCGAGCTGACCGAG
ATCCTCGACGAGGACGGCCGGGGTTTCGACGGATACGGCATCGGCGGCGCGCTGGAGAAGCAGAACCTCGCCACCATCGT
CGGCTGGGTCACCAGCGAACTGCCCGACGACAAACCGCGCCACCTGCTGGGGATCAGCGAACCCGACGACCTGTTCGCCG
CGGTCGAGGCCGGCGCCGACACCTTCGACTGTGTGTCGCCGTCACGGGTCGCCCGCAACGCCGCTGTGTACTCGGCGACC
GGGCGGTACAACATCACCGGTTCGCGCTACCGCCGCGACTTCACGCCGATCGACGCCGAATGCGACTGCTACACCTGCGC
TCACTACACCCGCGGGTACATCCACCACCTGTTCAAGGCCAAGGAGATGCTGGCCTCGACGCTGTGCACAATCCACAACG
AACGCTTCGTCGTCCGCCTCGTCGACCAGATCCGGGCCTCGATCGTCGCGGGCAACTTCGACGAACTCCGCGAGCACGTG
CTCGGGCGGTACTACGCCGCGCGTGGACAATGA

Upstream 100 bases:

>100_bases
GATCCGCGAAGCGGGACACTTCCTGCAGGAGGACGCGGGCGAGGACCTCGCCCGGGCGGTCGTCGAGTTCTGCCGCAGGC
GGTCCTGTTGAATACCTGAC

Downstream 100 bases:

>100_bases
GTGGATGACCGCGACCGCGGACCCCCGGGCGCTGCTCGGCCGGCTGCTCGACCCGGCCAACCGGGCCGACCCCTACCCGC
TGTACGCGGACTTCCGGGCG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDQQFFTVEAELPGRRGRAGVIRTPHGEIRTPAFIAVGTQATVKAVLPETMRELGAQAVLANAYHLYLQPGPDVVAAAGG
LGAFMNWPGPTFTDSGGFQVLSLGAGFRKVLAMDANRVQADDVIAKGKERLAVVDDDGVTFRSHLNGTTHRFTPEVSIGI
QHQLGADIIFAFDELTTLVNTRGYQEDSVRRTHRWAERCLAEHRKLSLENHEKPPQALFGVVQGAQYEDLRRQAARELTE
ILDEDGRGFDGYGIGGALEKQNLATIVGWVTSELPDDKPRHLLGISEPDDLFAAVEAGADTFDCVSPSRVARNAAVYSAT
GRYNITGSRYRRDFTPIDAECDCYTCAHYTRGYIHHLFKAKEMLASTLCTIHNERFVVRLVDQIRASIVAGNFDELREHV
LGRYYAARGQ

Sequences:

>Translated_410_residues
MDQQFFTVEAELPGRRGRAGVIRTPHGEIRTPAFIAVGTQATVKAVLPETMRELGAQAVLANAYHLYLQPGPDVVAAAGG
LGAFMNWPGPTFTDSGGFQVLSLGAGFRKVLAMDANRVQADDVIAKGKERLAVVDDDGVTFRSHLNGTTHRFTPEVSIGI
QHQLGADIIFAFDELTTLVNTRGYQEDSVRRTHRWAERCLAEHRKLSLENHEKPPQALFGVVQGAQYEDLRRQAARELTE
ILDEDGRGFDGYGIGGALEKQNLATIVGWVTSELPDDKPRHLLGISEPDDLFAAVEAGADTFDCVSPSRVARNAAVYSAT
GRYNITGSRYRRDFTPIDAECDCYTCAHYTRGYIHHLFKAKEMLASTLCTIHNERFVVRLVDQIRASIVAGNFDELREHV
LGRYYAARGQ
>Mature_410_residues
MDQQFFTVEAELPGRRGRAGVIRTPHGEIRTPAFIAVGTQATVKAVLPETMRELGAQAVLANAYHLYLQPGPDVVAAAGG
LGAFMNWPGPTFTDSGGFQVLSLGAGFRKVLAMDANRVQADDVIAKGKERLAVVDDDGVTFRSHLNGTTHRFTPEVSIGI
QHQLGADIIFAFDELTTLVNTRGYQEDSVRRTHRWAERCLAEHRKLSLENHEKPPQALFGVVQGAQYEDLRRQAARELTE
ILDEDGRGFDGYGIGGALEKQNLATIVGWVTSELPDDKPRHLLGISEPDDLFAAVEAGADTFDCVSPSRVARNAAVYSAT
GRYNITGSRYRRDFTPIDAECDCYTCAHYTRGYIHHLFKAKEMLASTLCTIHNERFVVRLVDQIRASIVAGNFDELREHV
LGRYYAARGQ

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=396, Percent_Identity=36.1111111111111, Blast_Score=262, Evalue=4e-70,
Organism=Escherichia coli, GI1786607, Length=384, Percent_Identity=39.3229166666667, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17544680, Length=389, Percent_Identity=33.6760925449871, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI25146502, Length=149, Percent_Identity=28.1879194630872, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=402, Percent_Identity=35.5721393034826, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI21355311, Length=243, Percent_Identity=27.1604938271605, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45233; Mature: 45233

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQFFTVEAELPGRRGRAGVIRTPHGEIRTPAFIAVGTQATVKAVLPETMRELGAQAVL
CCCCEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCHHHEE
ANAYHLYLQPGPDVVAAAGGLGAFMNWPGPTFTDSGGFQVLSLGAGFRKVLAMDANRVQA
ECEEEEEECCCCCEEEECCCCCHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCH
DDVIAKGKERLAVVDDDGVTFRSHLNGTTHRFTPEVSIGIQHQLGADIIFAFDELTTLVN
HHHHHCCCCEEEEECCCCCEEHHHCCCCCEEECCCEEECCCHHCCCCEEEHHHHHHHHHH
TRGYQEDSVRRTHRWAERCLAEHRKLSLENHEKPPQALFGVVQGAQYEDLRRQAARELTE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
ILDEDGRGFDGYGIGGALEKQNLATIVGWVTSELPDDKPRHLLGISEPDDLFAAVEAGAD
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHCCCC
TFDCVSPSRVARNAAVYSATGRYNITGSRYRRDFTPIDAECDCYTCAHYTRGYIHHLFKA
CCCCCCHHHHHHCCCEEECCCEEECCHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
KEMLASTLCTIHNERFVVRLVDQIRASIVAGNFDELREHVLGRYYAARGQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQQFFTVEAELPGRRGRAGVIRTPHGEIRTPAFIAVGTQATVKAVLPETMRELGAQAVL
CCCCEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCHHHEE
ANAYHLYLQPGPDVVAAAGGLGAFMNWPGPTFTDSGGFQVLSLGAGFRKVLAMDANRVQA
ECEEEEEECCCCCEEEECCCCCHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCH
DDVIAKGKERLAVVDDDGVTFRSHLNGTTHRFTPEVSIGIQHQLGADIIFAFDELTTLVN
HHHHHCCCCEEEEECCCCCEEHHHCCCCCEEECCCEEECCCHHCCCCEEEHHHHHHHHHH
TRGYQEDSVRRTHRWAERCLAEHRKLSLENHEKPPQALFGVVQGAQYEDLRRQAARELTE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
ILDEDGRGFDGYGIGGALEKQNLATIVGWVTSELPDDKPRHLLGISEPDDLFAAVEAGAD
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHCCCC
TFDCVSPSRVARNAAVYSATGRYNITGSRYRRDFTPIDAECDCYTCAHYTRGYIHHLFKA
CCCCCCHHHHHHCCCEEECCCEEECCHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
KEMLASTLCTIHNERFVVRLVDQIRASIVAGNFDELREHVLGRYYAARGQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA