BacMap

Definition	Mycobacterium sp. MCS chromosome, complete genome.
Accession	NC_008146
Length	5,705,448

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The map label for this gene is yxjF [H]

Identifier: 108801664

GI number: 108801664

Start: 4982152

End: 4982898

Strand: Reverse

Name: yxjF [H]

Synonym: Mmcs_4701

Alternate gene names: 108801664

Gene position: 4982898-4982152 (Counterclockwise)

Preceding gene: 108801665

Following gene: 108801663

Centisome position: 87.34

GC content: 66.67

Gene sequence:

>747_bases
ATGAGGGATCTCGCCGGGCGCACCGCGCTGGTGACAGGCGGTGCCAGCGGGATCGGGGAGGCGTGTGCACGCGAACTCGC
CGCCCGCGGTGCCACCGTCACCGTCGCCGACCGCGACGAAACGGCCGCTACCGCACTGGCCGACGAGATCGGCGGCAGCG
CCTGGGTGGTGGACCTGCTCGACGTGTCGGCGCTCGAAGGTCTCGCACTGGACACCGACATCCTGGTGAACAACGCCGGT
GTGCAGCGCATCGACCCGATCGTCGACTTCGCCCCGGAGAACTTCCGGATGCTGATGACGCTGATGGTCGAGGCGCCGTT
CCTGCTCATCCGCGCGGCGCTGCCGTACATGTACGCGCAGAAATTCGGCCGGGTCATCAACATCTCGTCGGTGCACGGGT
TGCGCGCCTCGGAGTACAAGGTCGGCTACGTCACCGCCAAGCACGCGCTCGAGGGGCTGTCGAAGGTGACCGCGCTGGAA
GGCGGACCGCATCAGGTGACCAGTAACTGCGTCAACCCCGGCTACGTGCGAACCCCGCTGGTCACCAAACAGATAGCCGA
TCAGGCCAAGGTGCACGGCATCGGTGAAGATGACGTGGTGACCCAGATCCTGCTCAAGGAGAGCGCCATCAAGCGACTCG
TCGAACCGTCCGAAGTCGGATCGCTGGTGGGCTGGTTGGCCTCGCCACAGTCCGGCATGGTGACCGGTGCGGCCTACACC
ATGGACGGCGGATGGAGCGCCCGGTGA

Upstream 100 bases:

>100_bases
TCGCGGTCTTCTTCACCCGTGAGACCAAGGGCATCGACCTGCACGACGTGGACCGGGCCGACCGCGAGGACCTCGCCCGC
GAGGCGGCCAGGAGCGGCGC

Downstream 100 bases:

>100_bases
GCGAACCGCAGTGGACGCCGACCGAGGACGACATCGCCGGCGCTCGCGTCACCGACTTCGCCCGCTTCGTCCAGCAGCGC
ACCGGCGCCGACGTATCCGA

Product: short-chain dehydrogenase/reductase SDR

Products: acetoacetate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRDLAGRTALVTGGASGIGEACARELAARGATVTVADRDETAATALADEIGGSAWVVDLLDVSALEGLALDTDILVNNAG
VQRIDPIVDFAPENFRMLMTLMVEAPFLLIRAALPYMYAQKFGRVINISSVHGLRASEYKVGYVTAKHALEGLSKVTALE
GGPHQVTSNCVNPGYVRTPLVTKQIADQAKVHGIGEDDVVTQILLKESAIKRLVEPSEVGSLVGWLASPQSGMVTGAAYT
MDGGWSAR

Sequences:

>Translated_248_residues
MRDLAGRTALVTGGASGIGEACARELAARGATVTVADRDETAATALADEIGGSAWVVDLLDVSALEGLALDTDILVNNAG
VQRIDPIVDFAPENFRMLMTLMVEAPFLLIRAALPYMYAQKFGRVINISSVHGLRASEYKVGYVTAKHALEGLSKVTALE
GGPHQVTSNCVNPGYVRTPLVTKQIADQAKVHGIGEDDVVTQILLKESAIKRLVEPSEVGSLVGWLASPQSGMVTGAAYT
MDGGWSAR
>Mature_248_residues
MRDLAGRTALVTGGASGIGEACARELAARGATVTVADRDETAATALADEIGGSAWVVDLLDVSALEGLALDTDILVNNAG
VQRIDPIVDFAPENFRMLMTLMVEAPFLLIRAALPYMYAQKFGRVINISSVHGLRASEYKVGYVTAKHALEGLSKVTALE
GGPHQVTSNCVNPGYVRTPLVTKQIADQAKVHGIGEDDVVTQILLKESAIKRLVEPSEVGSLVGWLASPQSGMVTGAAYT
MDGGWSAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=32, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=32.1285140562249, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI32483357, Length=261, Percent_Identity=31.0344827586207, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI59889578, Length=233, Percent_Identity=30.4721030042918, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=28.0155642023346, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI5031737, Length=263, Percent_Identity=30.0380228136882, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4504505, Length=259, Percent_Identity=29.3436293436293, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=26.5151515151515, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI20149619, Length=203, Percent_Identity=27.0935960591133, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40254992, Length=249, Percent_Identity=27.710843373494, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI33667109, Length=192, Percent_Identity=32.8125, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI10190704, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=31.3513513513514, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=35, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=30.2788844621514, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788459, Length=256, Percent_Identity=35.15625, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=32.8063241106719, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=30.5882352941176, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082160, Length=259, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789208, Length=256, Percent_Identity=27.34375, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=28.8537549407115, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=29.2307692307692, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI2367175, Length=255, Percent_Identity=29.0196078431373, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789057, Length=254, Percent_Identity=26.7716535433071, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787526, Length=193, Percent_Identity=30.0518134715026, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=258, Percent_Identity=30.2325581395349, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=30.859375, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17555706, Length=252, Percent_Identity=29.3650793650794, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=29.5719844357977, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17568967, Length=228, Percent_Identity=29.3859649122807, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=254, Percent_Identity=28.3464566929134, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=255, Percent_Identity=29.8039215686275, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=273, Percent_Identity=30.03663003663, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=195, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=194, Percent_Identity=28.3505154639175, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=192, Percent_Identity=28.6458333333333, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=198, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=25.4752851711027, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555612, Length=215, Percent_Identity=31.1627906976744, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=279, Percent_Identity=27.5985663082437, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=198, Percent_Identity=32.3232323232323, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=281, Percent_Identity=27.0462633451957, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=280, Percent_Identity=26.4285714285714, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=272, Percent_Identity=27.2058823529412, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=272, Percent_Identity=26.8382352941176, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=186, Percent_Identity=31.1827956989247, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=30.5882352941176, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=31.2, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=33.7349397590361, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571526, Length=260, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357041, Length=260, Percent_Identity=29.6153846153846, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355319, Length=261, Percent_Identity=27.2030651340996, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI18859837, Length=187, Percent_Identity=34.2245989304813, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=28.6821705426357, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644337, Length=255, Percent_Identity=32.156862745098, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24584744, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574402, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358495, Length=226, Percent_Identity=24.7787610619469, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584748, Length=188, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI161076933, Length=188, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011294
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.30

Molecular weight: Translated: 26121; Mature: 26121

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00061 ADH_SHORT ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLAGRTALVTGGASGIGEACARELAARGATVTVADRDETAATALADEIGGSAWVVDLL
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCEEEHHHH
DVSALEGLALDTDILVNNAGVQRIDPIVDFAPENFRMLMTLMVEAPFLLIRAALPYMYAQ
HHHHHCCCCCCHHEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFGRVINISSVHGLRASEYKVGYVTAKHALEGLSKVTALEGGPHQVTSNCVNPGYVRTPL
HHCCEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEECCH
VTKQIADQAKVHGIGEDDVVTQILLKESAIKRLVEPSEVGSLVGWLASPQSGMVTGAAYT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEE
MDGGWSAR
ECCCCCCC
>Mature Secondary Structure
MRDLAGRTALVTGGASGIGEACARELAARGATVTVADRDETAATALADEIGGSAWVVDLL
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCEEEHHHH
DVSALEGLALDTDILVNNAGVQRIDPIVDFAPENFRMLMTLMVEAPFLLIRAALPYMYAQ
HHHHHCCCCCCHHEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFGRVINISSVHGLRASEYKVGYVTAKHALEGLSKVTALEGGPHQVTSNCVNPGYVRTPL
HHCCEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEECCH
VTKQIADQAKVHGIGEDDVVTQILLKESAIKRLVEPSEVGSLVGWLASPQSGMVTGAAYT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEE
MDGGWSAR
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-3-hydroxybutanoate; NAD+

Specific reaction: (R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]