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The map label for this gene is shiA [H]

Identifier: 108801665

GI number: 108801665

Start: 4982895

End: 4984280

Strand: Reverse

Name: shiA [H]

Synonym: Mmcs_4702

Alternate gene names: 108801665

Gene position: 4984280-4982895 (Counterclockwise)

Preceding gene: 108801667

Following gene: 108801664

Centisome position: 87.36

GC content: 66.81

Gene sequence:

>1386_bases
ATGAGCACGCAGAATCCCTCACCACCGGACACCAGCCCCCCGGACGCGATCACCACCGGCCTCAAACGCGTGGTCGTGGC
CTCGATGGCTGGCACCGTCGTCGAGTGGTACGAGTTCTTCCTCTACGGCACCGCCGCCACGCTGGTGTTCAACAAGATCT
TCTTCTCGGAGACCACCAGCGAGCTCAACGCGATCTTCCTCGCGTTCGCGACGTACGCGGTGGGCTTCGTCGCCCGCCCG
CTCGGAGGCGTGGTCTTCGGGCATTTCGGCGACAAGTACGGCCGTAAGAAGCTCCTCCAGTTCAGCCTCCTGCTCGTCGG
TGCCGCCACCTTCGCGATGGGCTGCCTGCCGACGTACGGCCAGATCGGCTACTGGGCACCGGCCCTGCTGGTCGCGTTGC
GCTTCATCCAGGGCTTCGCCGTCGGCGGAGAGTGGGGCGGCGCGGTCCTGCTGGTCGCCGAACACAGCCCCAACCGGGAA
CGCGGCTTCTGGGCCAGCTGGCCGCAGGCCGGTGTGCCCGTCGGCAACCTGCTCGCCACCATCGTGCTGCTCGTCCTGAC
GTCGACGCTGCCCGAGACGTCGTTCCTGTCGTGGGGCTGGCGTGTGGCGTTCTGGCTGTCCGCGGTGGTGGTGCTGATCG
GCTACTACATCCGCACCAAGGTCACCGACGCGCCGATCTTCGTCGCCGCCCAGCAGGAGGTGGAGCGGGCCAAGGCCACT
TCGTATGGGGTGGTCGAGGTGCTCAAGCGCTACCCGCGCGGCGTCTTCACCGCGATGGGCCTGCGGTTCGGCGAGAACAT
CATGTACTACCTCGTGGTCACCTTCTCGATCACGTATCTGAAGGTCCAGGTCGGCGCCGACACCAGCGACATCCTCTGGT
ACCTGCTGGTGGCGCACCTGATCCACTTCGCGGTGGTGCCGCTCGTGGGGTGGCTGTCGGACCGGTTCGGCCGCCGCCCG
GTGTACGTGGTGGGCGCGGTCCTCGGCGCGACGTGGGGCTTCTTCGCGTTCCCGATGATGAACAGCGGTAGCTATGTCGT
CGTGACGGCAGCGGTCAGCATCGGCCTGATGATCCACGCGCTGATGTACGCGCCGCAGCCGGCGATCATGGCGGAGATGT
TCCCGACCCGGATGCGGTATTCCGGTGTCTCCCTCGGCTATCAGGTCACCTCGATCGTGGCCGGATCACTGGCGCCGCTC
ATCGCGGTGAAACTGCTCGACATCTACGGCTCGTCGGTGCCGATCGCGATCTATCTGGCCGTGGCGTGTGCGATCACGTT
GGTCGCGGTCTTCTTCACCCGTGAGACCAAGGGCATCGACCTGCACGACGTGGACCGGGCCGACCGCGAGGACCTCGCCC
GCGAGGCGGCCAGGAGCGGCGCATGA

Upstream 100 bases:

>100_bases
GCCATTGCGGGCGGGTCCGGCGTGCATGAATACTCACAGCCATGTGGGGACAGTCACACACCACCCGTCTGTATGCCCCC
GGTCATGAACGGAGCCCTCG

Downstream 100 bases:

>100_bases
GGGATCTCGCCGGGCGCACCGCGCTGGTGACAGGCGGTGCCAGCGGGATCGGGGAGGCGTGTGCACGCGAACTCGCCGCC
CGCGGTGCCACCGTCACCGT

Product: general substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSTQNPSPPDTSPPDAITTGLKRVVVASMAGTVVEWYEFFLYGTAATLVFNKIFFSETTSELNAIFLAFATYAVGFVARP
LGGVVFGHFGDKYGRKKLLQFSLLLVGAATFAMGCLPTYGQIGYWAPALLVALRFIQGFAVGGEWGGAVLLVAEHSPNRE
RGFWASWPQAGVPVGNLLATIVLLVLTSTLPETSFLSWGWRVAFWLSAVVVLIGYYIRTKVTDAPIFVAAQQEVERAKAT
SYGVVEVLKRYPRGVFTAMGLRFGENIMYYLVVTFSITYLKVQVGADTSDILWYLLVAHLIHFAVVPLVGWLSDRFGRRP
VYVVGAVLGATWGFFAFPMMNSGSYVVVTAAVSIGLMIHALMYAPQPAIMAEMFPTRMRYSGVSLGYQVTSIVAGSLAPL
IAVKLLDIYGSSVPIAIYLAVACAITLVAVFFTRETKGIDLHDVDRADREDLAREAARSGA

Sequences:

>Translated_461_residues
MSTQNPSPPDTSPPDAITTGLKRVVVASMAGTVVEWYEFFLYGTAATLVFNKIFFSETTSELNAIFLAFATYAVGFVARP
LGGVVFGHFGDKYGRKKLLQFSLLLVGAATFAMGCLPTYGQIGYWAPALLVALRFIQGFAVGGEWGGAVLLVAEHSPNRE
RGFWASWPQAGVPVGNLLATIVLLVLTSTLPETSFLSWGWRVAFWLSAVVVLIGYYIRTKVTDAPIFVAAQQEVERAKAT
SYGVVEVLKRYPRGVFTAMGLRFGENIMYYLVVTFSITYLKVQVGADTSDILWYLLVAHLIHFAVVPLVGWLSDRFGRRP
VYVVGAVLGATWGFFAFPMMNSGSYVVVTAAVSIGLMIHALMYAPQPAIMAEMFPTRMRYSGVSLGYQVTSIVAGSLAPL
IAVKLLDIYGSSVPIAIYLAVACAITLVAVFFTRETKGIDLHDVDRADREDLAREAARSGA
>Mature_460_residues
STQNPSPPDTSPPDAITTGLKRVVVASMAGTVVEWYEFFLYGTAATLVFNKIFFSETTSELNAIFLAFATYAVGFVARPL
GGVVFGHFGDKYGRKKLLQFSLLLVGAATFAMGCLPTYGQIGYWAPALLVALRFIQGFAVGGEWGGAVLLVAEHSPNRER
GFWASWPQAGVPVGNLLATIVLLVLTSTLPETSFLSWGWRVAFWLSAVVVLIGYYIRTKVTDAPIFVAAQQEVERAKATS
YGVVEVLKRYPRGVFTAMGLRFGENIMYYLVVTFSITYLKVQVGADTSDILWYLLVAHLIHFAVVPLVGWLSDRFGRRPV
YVVGAVLGATWGFFAFPMMNSGSYVVVTAAVSIGLMIHALMYAPQPAIMAEMFPTRMRYSGVSLGYQVTSIVAGSLAPLI
AVKLLDIYGSSVPIAIYLAVACAITLVAVFFTRETKGIDLHDVDRADREDLAREAARSGA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=432, Percent_Identity=37.962962962963, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=38.7173396674584, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=33.1002331002331, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=31.2941176470588, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=24.331550802139, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082231, Length=291, Percent_Identity=23.3676975945017, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50109; Mature: 49978

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
STQNPSPPDTSPPDAITTGLKRVVVASMAGTVVEWYEFFLYGTAATLVFNKIFFSETTS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELNAIFLAFATYAVGFVARPLGGVVFGHFGDKYGRKKLLQFSLLLVGAATFAMGCLPTYG
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QIGYWAPALLVALRFIQGFAVGGEWGGAVLLVAEHSPNRERGFWASWPQAGVPVGNLLAT
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
IVLLVLTSTLPETSFLSWGWRVAFWLSAVVVLIGYYIRTKVTDAPIFVAAQQEVERAKAT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHC
SYGVVEVLKRYPRGVFTAMGLRFGENIMYYLVVTFSITYLKVQVGADTSDILWYLLVAHL
CHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHH
IHFAVVPLVGWLSDRFGRRPVYVVGAVLGATWGFFAFPMMNSGSYVVVTAAVSIGLMIHA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHH
LMYAPQPAIMAEMFPTRMRYSGVSLGYQVTSIVAGSLAPLIAVKLLDIYGSSVPIAIYLA
HHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VACAITLVAVFFTRETKGIDLHDVDRADREDLAREAARSGA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]